Effects of Astragalus injection on neuronal apoptosis and expression of JNK3 in rat hippocampus after cerebral ischemia reperfusion     

LIU Sha-sha  GAO Wei-juan  QIAN Tao  ZHANG Xia 《园艺学报》2012,28(9):1582-1588

AIM:To investigate the effects of Astragalus injection on neuronal apoptosis and expression of c-Jun N-terminal kinase 3(JNK3) in the rat hippocampus after cerebral ischemia reperfusion. METHODS:The rat model of cerebral ischemia reperfusion was set up by a four-vessel occlusion method. The SD rats were randomly divided into 4 groups:sham operation group, cerebral ischemia reperfusion group(model group), cerebral ischemia reperfusion+Astragalus injection group(Astragalus injection group) and cerebral ischemia reperfusion+vehicle group(vehicle group). The rats in model group, Astragalus injection group and vehicle group after transient global cerebral ischemia(30 min) were then divided into 7 subgroups according to the reperfusion time of 0 h, 0.5 h, 2 h, 6 h, 24 h, 72 h and 120 h. The apoptosis of the neuron in the hippocampus was measured by the method of TUNEL staining. The expression of JNK3 at mRNA and protein levels was determined by real-time PCR and Western blotting,respectively. RESULTS:Compared with sham operation group, the number of apoptotic neurons increased in model group(P<0.05). Compared with model group, the number of apoptotic neurons decreased obviously in Astragalus injection group(P<0.05). Compared with sham operation group, the expression of JNK3 at mRNA and protein levels in the hippocampus increased obviously in model group at all time points except 120 h(P<0.05). Compared with model group, the expression of JNK3 at mRNA and protein levels in the hippocampus decreased obviously in Astragalus injection group at all time points except 120 h(P<0.05). CONCLUSION:Astragalus injection decreases neuronal apoptosis in rat hippocampus after cerebral ischemia reperfusion by inhibiting the expression of JNK3 at mRNA and protein levels.  相似文献   

A control system for vehicle applications of high-blend methanol gasoline     

YANG Fan  ZHANG Jun  HU Pei feng 《保鲜与加工》2012,(4):133-138

The ordinary gasoline engines could not be fueled by high-blend methanol directly. A MCU-based high-blend methanol gasoline control system is designed to extend the injection pulse width. PROTEUS simulation and test bench are validated. Flyer M-TCE engines burning M85 go smoothly. The results indicate that the high-blend methanol gasoline control system caneffectively control the engine injection mass without changing the original Electronic Control Unit , and make it feasible to apply high-blend methanol gasoline to the ordinary gasoline engines.  相似文献   

Effect of cattle slurry application techniques on N2O and NH3 emissions from a loamy soil     

Christina Herr  Thomas Mannheim  Torsten Müller  Reiner Ruser 《植物养料与土壤学杂志》2019,182(6):964-979

We determined N₂O fluxes from an unfertilized control (CON), from a treatment with mineral N‐fertilizer (MIN), from cattle slurry with banded surface application and subsequent incorporation (INC), and from slurry injection (INJ) to silage maize (Zea mays, L.) on a Haplic Luvisol in southwest Germany. In both years, amount of available N (total N fertilized + N_min content before N application) was 210 kg N ha^?1. In the slurry treatment of the 1^st year, 140 kg N ha^?1 were either injected or incorporated, whereas 30 kg N ha^?1 were surface applied to avoid destruction of the maize plants. In the 2^nd year, all fertilizers were applied with one single application. We calculated greenhouse gas emissions (GHG) on field level including direct N₂O emissions (calculated from the measured flux rates), indirect N₂O emissions (NH₃ and ${\mathrm{NO}}_3^{-}$ induced N₂O emission), net CH₄ fluxes, fuel consumption and pre‐chain emissions from mineral fertilizer. NH₃ losses were measured in the 2^nd year using the Dräger‐Tube Method and estimated for both years. NH₃ emission was highest in the treatment without incorporation. It generally contributed less than 5% of the greenhouse gas (GHG) emission from silage maize cultivation. The mean area‐related N₂O emission, determined with the closed chamber method was 2.8, 4.7, 4.4 and 13.8 kg N₂O‐N ha^?1 y^?1 for CON, MIN, INC, and INJ, respectively. Yield‐related N₂O emission showed the same trend. Across all treatments, direct N₂O emission was the major contributor to GHG with an average of 79%. Trail hose application with immediate incorporation was found to be the optimum management practice for livestock farmers in our study region.  相似文献   

鹅细小病毒VP3基因疫苗两种不同免疫方式对细胞免疫的影响     

车茜程安春  汪铭书黎敏  韩新锋卢菲刘晓东  陈孝跃 《中国兽医科技》2007,37(5):401-405

将构建的鹅细小病毒（GPV）VP3基因疫苗（pcDNA-GPV-VP3）转染鹅胚成纤维细胞（GEF），每隔12h检测VP3蛋白的表达情况，同时以不同剂量分别通过基因枪轰击和肌肉注射免疫28日龄健康雏鹅，于免疫后第3、7、14、21、28、35、49、63、77和105d采血，进行淋巴细胞增殖试验（MTT法）。结果，转染后第24h即可在GEF中检测到GPVVP3蛋白，第60h表达量达到高峰。试验组鹅外周血T淋巴细胞D490nm值在免疫后第35d达到最大。肌肉注射组和基因枪组免疫后第14～63d、第7～63d的D490nm值分别与PBS对照组差异板显著；肌肉注射组及基因枪组免疫后第21～49d的D490nm值显著高于弱毒疫苗对照组；肌肉注射组及基因枪组免疫后第14～63d的D490nm值板显著高于空质粒对照组。表明，基因枪轰击和肌肉注射pcDNA—GPV—VP3均能诱导雏鹅产生良好的细胞免疫应答。  相似文献   

囊胚注射转基因ES细胞制作嵌合体的研究   总被引：2，自引：0，他引：2  

付志新  刘宗平  梁建兰  李善刚  陈学进 《畜牧兽医学报》2007,38(2):149-154

在小鼠胚胎成纤维细胞制作的饲养层上培养并成功的维持了携带LacZ基因的胚胎干细胞系（S8），在此基础上，以S8为供体细胞，以远交系昆明白小鼠3．5d胚胎为受体，通过显微注射法将供体细胞转移到受体的囊胚腔内，经过恢复培养，移植到代孕鼠昆明白雌鼠的子宫中；后代在嵌合体出生一周后进行判定。本试验用8～13代的S8细胞共注射胚胎597枚，经1～3h恢复培养，有585枚胚胎重新具有膨大的囊胚腔，细胞轮廓分明，滋养层细胞间连接也清晰可见，胚胎成活率为97％；胚胎移植后，代孕母鼠共移植胚胎228枚，经17～19d的妊娠期后，产仔37只（2只死胎），产仔率为16％；有35只仔鼠（雄鼠18只，雌鼠17只）存活到可以判断毛色，共获得8只S8细胞毛色嵌合体小鼠，嵌合体的产生率为21．6％。结果表明用S8细胞经囊胚注射后能够获得嵌合体，并且嵌合体明显发生了性偏离现象。本试验为国内利用囊胚注射携带LacZ基因的胚胎干细胞获得嵌合体小鼠的首例报道。  相似文献   

Protective effects of Shengfu injections on limbs in ischemia/reperfusion injury     

HUANG Wei  ZHANG Ying-peng  ZHONG Bo  WANG Su-wei 《园艺学报》2007,23(3):540-542

AIM:To study the protective effects of Shengfu injections on the limbs in ischemia/reperfusion injury. METHODS:30 healthy rats at the same age were divided into 5 groups randomly. Group A was the simple reperfusion of ischemic limbs. Group B and C were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg. Group D and E were injected with the Shengfu injections through femoral arteries at doses of 10 mL/kg and 20 mL/kg right after reperfusions. Blood samples were collected from femoral veins 1 h before femoral vessels were blocked, 1 h and 2 h after reperfusions in all animals. The concentrations of the CPK, GOT, SOD and MDA in the blood samples were determined. RESULTS:MDA increased, SOD, CPK, GOT decreased markedly in the therapeutic groups.CONCLUSION:The Shengfu injections surely protected the limbs from ischemia/reperfusion injury, the better effects was observed when the drug was administered before operation at the dose of 20 mL/kg.  相似文献   

胞质内精子注射及其影响因素的研究     

王玉琴  王占彬  祁艳霞  吴秋珏 《中国畜牧兽医》2007,34(7):35-37

作者综述了胞质内精子注射(ICSI)技术的原理及其在人及其他动物上的研究应用，阐述卵子、精子操作技术等对ICSI结局的影响，指出目前存在的问题和应用前景。  相似文献   

影响猪ICSI转基因技术效率的主要因素研究   总被引：2，自引：0，他引：2  

刘成军  曾申明  万鹏程  郝泽东 《中国畜牧杂志》2006,42(17):7-9

以猪体外成熟卵子和冷冻解冻的死精子为材料,以pEGFP-N1为模式基因,探讨注射台温度、激活后6-DMAP的处理和精子与PEGFP-N1孵育液添加BSA(牛血清白蛋白)对精子胞质内注射(ICSI)转基因效率的影响。结果表明:注射台温度为30℃时的阳性率为40.07%,而38.5℃时为20.97%,差异极显著(P<0.01)。添加BSA的囊胚转基因率为55.56%,对照组为33.33%,差异极显著(P<0.01)。6-DMAP处理组与对照组的转基因率分别为52.53%和26.25%,差异极显著(P<0.01);而且6-DMAP处理组的囊胚率(9.96%)显著高于(P<0.05)对照组(2.30%)。研究表明注射台温度对转基因效率有明显影响,温度高转基因率低;精子与PEGFP-N1孵育液添加BSA对转基因胚胎发育有一定促进和保护作用,有利于提高囊胚转基因率;激活后用6-DMAP处理能提高转基因率和囊胚率。  相似文献   

双黄连注射液治疗母畜子宫内膜炎的临床试验   总被引：4，自引：0，他引：4  

汪勇  顺克巧  张国红 《中国兽药杂志》2006,40(3):53-55

临床选取自然发生子宫内膜炎的猪只236头，羊241只，牛180头。将发病母畜随机分为高、中、低剂量组，药物对照组和阳性对照组，分别采用肌肉注射和子宫灌注两种给药途径交替给药，一日一次，连续3次给药后，停药观察7d。结果双黄连注射液高剂量组与中剂量组对子宫内膜炎的疗效优于化学药物对照组，差异均显著（P〈0．05）；低剂量组治疗效果与化学药物组差异不显著（P〉0．05）。双黄连注射液对家畜无毒副作用，而且还能增强家畜的抵抗力，因此可作为治疗母畜子宫内膜炎的首选药物。  相似文献   

氯胺磷树干注药防治松褐天牛试验   总被引：1，自引：0，他引：1  

刘际建  李超英  冯思平  吴仕军 《农药科学与管理》2006,27(12):35-36,25

利用30%氯胺磷乳油、2%阿维菌素乳油等对危害马尾松的松褐天牛进行了树干注射防治试验并与40%氧乐果乳油进行了对比。结果表明30%氯胺磷乳油与40%氯乐果乳油相比对松褐天牛有更好的防治效果。按马尾松树干胸径计,30%氯胺磷注乳油注药量0.7mL/cm,30d防效达95.10%。2%阿维菌素乳油防效差,30d防效仅为32.84%。  相似文献