Transformation of lily by Agrobacterium     

Simon A. Langeveld  Merel M. Gerrits  Anton F. L. M. Derks  Piet M. Boonekamp  John F. Bol 《Euphytica》1955,85(1-3):97-100

Summary Lily cv. Harmony was inoculated with several Agrobacterium strains to study its susceptibility to Agrobacterium infection and transformation. Tumorous tissue formation on inoculated stem internodes of sterile-grown plantlets, as well as expression of a -glucuronidase marker gene interrupted by an intron in cells of inoculated stem nodes, indicate that the monocotyledon Lilium is a host for Agrobacterium.  相似文献   

Floral biology and artificial pollination in Dioscorea alata L.     

K. Abraham  P. Gopinathan Nair 《Euphytica》1990,48(1):45-51

Summary Floral biology of Dioscorea alata L. was studied. Anthesis occurred in male flowers by 12.30 h and in female flowers between 13.00 h and 14.30 h. Male flowers remained open for 4–5 hours and female flowers for 9–11 days.Natural pollination was practically absent but parthenocarpic fruit development was observed in rare cases. For hand pollination a simple and efficient pencil method was standardised. Pollen remained viable for 4–5 hours whereas stigma receptivity lasted for 9–10 days. The optimum time of pollination was found to be between 12.00 h and 15.00 h. Several factors like high relative humidity, atmospheric temperatures below 30°C and non-bagging the female flowers were found to promote fruit set.  相似文献   

13-Acetoxysarcocrassolide Induces Apoptosis on Human Gastric Carcinoma Cells Through Mitochondria-Related Apoptotic Pathways: p38/JNK Activation and PI3K/AKT Suppression     

Ching-Chyuan Su  Jeff Yi-Fu Chen  Zhong-Hao Din  Jui-Hsin Su  Zih-Yan Yang  Yi-Jen Chen  Robert Y.L. Wang  Yu-Jen Wu 《Marine drugs》2014,12(10):5295-5315

13-acetoxysarcocrassolide (13-AC), an active compound isolated from cultured Formosa soft coral Sarcophyton crassocaule, was found to possess anti-proliferative and apoptosis-inducing activities against AGS (human gastric adenocarcinoma cells) gastric carcinoma cells. The anti-tumor effects of 13-AC were determined by MTT assay, colony formation assessment, cell wound-healing assay, TUNEL/4,6-Diamidino-2-phenylindole (DAPI) staining, Annexin V-fluorescein isothiocyanate/propidium iodide (PI) staining and flow cytometry. 13-AC inhibited the growth and migration of gastric carcinoma cells in a dose-dependent manner and induced both early and late apoptosis as assessed by flow cytometer analysis. 13-AC-induced apoptosis was confirmed through observation of a change in ΔΨm, up-regulated expression levels of Bax and Bad proteins, down-regulated expression levels of Bcl-2, Bcl-xl and Mcl-1 proteins, and the activation of caspase-3, caspase-9, p38 and JNK. Furthermore, inhibition of p38 and JNK activity by pretreatment with SB03580 (a p38-specific inhibitor) and SP600125 (a JNK-specific inhibitor) led to rescue of the cell cytotoxicity of 13-AC-treated AGS cells, indicating that the p38 and the JNK pathways are also involved in the 13-AC-induced cell apoptosis. Together, these results suggest that 13-AC induces cell apoptosis against gastric cancer cells through triggering of the mitochondrial-dependent apoptotic pathway as well as activation of the p38 and JNK pathways.  相似文献   

共转化法获得蒙农杂种冰草转基因植株(征文)   总被引：4，自引：0，他引：4  

霍秀文  云锦凤  米福贵  魏建华  张辉 《中国农业科学》2006,39(10):1977-1983

本研究以冰草属（Agropyron Gaertn.）中的一个优质种间杂种——“蒙农杂种”冰草（A. cristatum хA.desertorum cv.‘Hycrest-Mengnong’ ）为材料，在以幼穗为外植体建立的冰草组织培养再生体系基础上，以调控脯氨酸生物合成最后一步的关键酶的突变体基因p5CS为目标基因，bar基因为筛选标记基因，采用共转化法利用基因枪轰击冰草幼穗诱导的愈伤组织获得转基因植株,为冰草的遗传改良提供新种质。PCR和Southern检测表明外源基因p5CS已整合到冰草植物基因组DNA中；RT-PCR检测表明目的基因已在冰草转基因植株的转录水平表达；p5CS基因的遗传转化率为0.11%。  相似文献   

新基因p7TP3剪切体1的克隆与亚细胞定位          下载免费PDF全文

陶明亮  袁菊  成军  靳亚平 《西北农业学报》2007,16(6):19-22

利用RT-PCR技术扩增丙型肝炎病毒p7蛋白反式调节基因3剪切体1（p7TP3剪切体1）开放阅读框序列。将目的基因片段插入pGEM-T载体中,经双酶切和测序鉴定后,定向克隆至荧光表达载体pEGFP-C1,EcoRI及BamHI双酶切鉴定。脂质体法转染HepG2细胞,荧光显微镜下观察确定其亚细胞定位。结果表明,p7TP3剪切体1片断长度为381 bp,与预期结果一致;亚细胞定位结果表明,该蛋白定位于细胞浆中。  相似文献   

Effect of heat treatments on alpha-amylase inhibitor activity in sorghum (Sorghum bicolour L.)     

V. H. Mulimani  D. Supriya 《Plant foods for human nutrition (Dordrecht, Netherlands)》1993,44(2):181-186

The application of dry heat to sorghum seeds and meal was not effective in inactivating the amylase inhibitory activity. Overnight soaking followed by heat treatment was more effective in destroying amylase inhibitory activity. Cooking the meal, raw seeds and soaked seeds drastically reduced the levels of -amylase inhibitory activity.  相似文献   

捻转血矛线虫Hc38保守结构域所在基因片段的真核表达载体构建     

郑金海  段正秀  荣光  薄新文  李娜  何立雄 《动物医学进展》2009,30(2)

根据捻转血矛线虫Hc38基因产物(登录号AY749124)保守结构域所在核酸序列设计1对引物,该引物包含Kozak序列、HindⅢ酶切位点、Xba Ⅰ酶切位点.以含有捻转血矛线虫Hc38基因保守结构域片段的pMD19-T为模板,经PCR扩增获得Hc38基因保守结构域片段,用HindⅢ、Xba Ⅰ双酶切该片段,回收后将此基因片段克隆至相同酶切回收后的真核表达载体pcDNA3.1(+)中,获得重组质粒pcD-NA3.1-Hc38.经酶切分析、序列测定和PCR鉴定,证实了重组质粒的正确性.  相似文献   

重度践踏对粤选匍匐翦股颖新品系38号坪用性状的影响     

吴立山  刘荣堂  周胜  贺怀刚 《草原与草坪》2009,(4):16-19

研究重度践踏下草坪草匍匐翦股颖新品系38号坪用性状的变化过程,包括自然高度、叶片长度、密度和地上植物量,结果表明,践踏末期,新品系38号自然高度低于对照品种28号（P〈0．05）,叶片长度差异不显著（P〉0．05）,密度高于对照品种28号（P-〈0．05）。灰色关联分析法综合评分结果表明,新品系38号的坪用性状好于对照品种28号。  相似文献   

EM38探测复垦土壤厚度分布的可行性研究     

蔡彩霞  林剑辉  孟繁佳  孙宇瑞  李道亮 《农业工程学报》2010,26(12):319-323

该文采用EM38电磁感应电导率仪测量辽宁省阜新市海州露天矿场复垦区一块面积为5 000 m2的覆土层的表观电导率,试探性研究表观电导率（ECa）与矿区排土场覆土厚度（TSLT）的相关性。测定结果证实ECa与相同点位剖面挖掘所得的深度数据有很好的负相关性,而两层土壤的电导率差值与上层土壤深度之间存在一定的正相关性。结合地统计克里格插值,得到了该复垦区覆土深度分布的预测趋势图。研究结论指出,基于EM38测量的表观电导率预测矿区复垦区覆土层厚度具有良好的可行性,为废弃矿区复垦质量的客观评估,提供了一种实用、简捷的工程检测 手段。  相似文献   

粟酒裂殖酵母tRNA前体3′末端加工酶Trz1p功能的研究     

苏文陈  赵振  冯振华  黄鹰 《安徽农业科学》2009,37(26):12430-12432

[目的]研究粟酒裂殖酵母tRNA前体3’末端加工酶Trzlp在体内的功能,为以后进一步探索Trzlp的功能奠定基础。[方法]构建Trz1’的表达载体pREP82x．trzl-5flag,通过醋酸锂转化法将pREP82x-trzl-5flag转入yYH1菌株和7k1‘的缺失菌株ySS0319中,并进行随机孢子分析。[结果]Trz1pC-端添加5FLAG仍能促进tRNA前体3’末端的加工成熟,但Trz1-5flag不能救活Trz1’的缺失菌株。[结论]粟酒裂殖酵母Trzlp具有tRNA前体3’末端加工功能。  相似文献