转植酸酶玉米生育期及秸秆还田期根际微生物数量和细菌菌群多样性   总被引：2，自引：0，他引：2  

张林森  杨正友  孙红炜  李凡  杨淑珂  段丽君  路兴波 《山东农业科学》2013,45(3)

在大田栽培条件下,以转植酸酶玉米10TPY005、非转植酸酶亲本玉米为试材,于2011年在玉米的不同生育期以及秸秆还田期对玉米根际土壤细菌、放线菌和真菌数量的变化情况进行了测定。结果表明:在同一时期同一种微生物数量在转植酸酶玉米、非转基因玉米及空白对照中基本相同;不同细菌菌群在不同时期内数量变化情况不同:氨化细菌的数量在生育期内明显高于秸秆还田期,好气性纤维素分解菌及好气性固氮菌的数量在生育期内明显低于秸秆还田期;整个生育期和秸秆还田期转植酸酶玉米3种群落特征参数(优势集中性、群落多样性、均匀度指数)均高于非转植酸酶玉米,同时生育期内3种生物群落特征参数也明显高于秸秆还田期。  相似文献   

不同磷酸二氢钙含量饲料中添加植酸酶对建鲤生长、磷利用、体组成和消化酶活性的影响     

秦巍仑  杨毅  冷向军  吴江  李小勤 《上海海洋大学学报》2015,24(3):383-390

以初始体重为(12.8±0.2)g的建鲤(Cyprinus carpio var.Jian)为研究对象,探讨了在不同磷酸二氢钙含量饲料中添加包被酸性植酸酶对建鲤生长性能、磷利用率、体组成和肠消化酶活性的影响。共设5组饲料,即磷酸二氢钙含量分别为2.5%、2.0%、1.6%的3组饲料,及在2.0%、1.6%磷酸二氢钙饲料中添加2 000U/kg植酸酶的两组饲料。90 d的养殖实验结果表明:2.5%磷酸二氢钙组具有最高鱼体增重率,最低饲料系数和最低全鱼脂肪含量;1.6%磷酸二氢钙组的生长性能最低,全鱼脂肪含量最高;在2.0%磷酸二氢钙饲料中添加植酸酶,对生长、磷储积率和全鱼组成均没有显著影响,但提高了磷表观消化率和肠脂肪酶活性;在1.6%磷酸二氢钙饲料中添加植酸酶显著提高了鱼体增重率、磷表观消化率、磷储积率和全鱼粗蛋白、灰分含量(P0.05),降低了饲料系数和全鱼粗脂肪含量(P0.05),但对肠消化酶活性没有显著影响。上述结果表明:在含1.6%磷酸二氢钙饲料中添加包被酸性植酸酶可促进建鲤生长,降低鱼体粗脂肪含量,提高磷表观消化率和磷储积率。  相似文献   

Effects of a new recombinant phytase on performance and mineral utilization of laying ducks fed phosphorus-deficient diets     

《The Journal of Applied Poultry Research》2009,18(2):284-291

An experiment was conducted to evaluate the effects of a new phytase supplementation in Jinding laying ducks fed different concentrations of non-phytate P (NPP) on production performance, mineral retention, and bone and plasma minerals. A 14-wk experiment was conducted using 200-d-old female laying ducks. A total of 1,000 laying ducks were randomly allocated to 5 treatments and fed 5 diets: a control diet that contained an adequate concentration of NPP (0.45%) and 4 diets that were deficient in NPP (0.38, 0.32, 0.25, and 0.18%, respectively) but supplemented with phytase at 500 U/kg. Decreasing the NPP content from 0.45 to 0.18% in the diets with phytase supplementation had no detrimental effects on performance. However, Cu and Zn retention was significantly lower (P < 0.05) for laying ducks consuming the 0.18% NPP diet with phytase supplementation. Likewise, laying ducks fed the 0.18% NPP diet had a significant reduction in bone ash, Ca, P, and Cu contents, and in serum P and Cu (P < 0.05). Furthermore, the decrease in NPP content in the diet with phytase supplementation significantly increased (P < 0.05) P retention. Therefore, with the supplementation of this novel phytase at 500 U, it is possible to reduce dietary concentrations of NPP to 0.25% and maintain the normal performance of laying ducks.  相似文献   

植酸酶的应用及研究进展     

李光霞  李宗伟 《畜牧与饲料科学》2009,30(3)

植酸及其盐在动物体内起着抗营养作用,能够降低饲料利用率,提高饲料成本.在饲料中添加植酸酶,可以使动物有效利用饲料中的磷,减少磷的排出,减轻环境污染.综述了植酸酶的来源、作用机理以及应用和研究现状.  相似文献   

Dietary protein level, microbial phytase, citric acid and their interactions on bone mineralization of Labeo rohita (Hamilton) juveniles     

Kartik Baruah  Asim K Pal  Narottam P Sahu  Kamal K Jain  Subhas C Mukherjee  & Dipesh Debnath 《Aquaculture Research》2005,36(8):803-812

A feeding trial was conducted for 60 days to study the effect of dietary protein, microbial phytase and citric acid on intestinal digesta pH, bone ash and bone mineral contents in Labeo rohita juveniles. Eight experimental diets were prepared in 2 × 2 × 2 factorial arrangement with crude protein levels (25% and 35%), microbial phytase (0 and 500 U kg^?1), and citric acid (0 and 3%). The 25% crude protein level feed was supplemented with phytase (U kg^?1) and citric acid (%) at the level of 0,0 (C₂₅); 500,0 (T₁); 0,3 (T₂); 500,3 (T₃), and 35% crude protein level feed at 0.0 (C₃₅); 500,0 (T₄); 0,3 (T₅) and 500,3(T₆) respectively. One hundred and twenty juveniles of L. rohita (av. wt. 12.61–13.72 g) were distributed randomly in eight treatments, each of with three replicates. Addition of citric acid in the 25% crude protein feed significantly decreased (P<0.001) feed pH with concurrent decrease in intestinal digesta pH (P<0.001) and increased the bone ash content (P<0.05) by 4.6%. An interaction between citric acid and phytase (P<0.05) was also observed for bone ash content. Increasing the dietary protein content from 25% to 35% significantly decreased (P<0.01) bone Zn content by 14.9%, which was more prominent with the addition of citric acid, resulting in significant interaction between protein and citric acid (P<0.05), but the bone Cu content was significantly increased (P<0.01) with increasing dietary protein content. Dietary supplementation of microbial phytase (500 U kg^?1) significantly increased (P<0.05) bone Na, Ca, K, P and Fe contents by 15%, 12.1%, 17.4%, 9.2% and 40.7%, respectively, whereas bone P and Mn content was significantly increased (P<0.05) by addition of citric acid (3%). Addition of phytase to plant‐based diets increased the bioavailability of minerals, thereby increasing bone mineralization. The effect of phytase was increased because of addition of citric acid (3%).  相似文献   

植酸酶在点带石斑鱼配合饲料中的应用   总被引：8，自引：0，他引：8  

阮成旭  袁重桂  石祥柱 《福建水产》2005,(1):13-16

在植物性饲料含量分别为12．7％和32．7％的点带石斑鱼的配合饲料中按比例添加植酸酶，通过32d的养殖，与对照组相比，结果显示添加植酸酶可以促进石斑鱼的生长，提高石斑鱼的相对增重率，降低饲料系数。  相似文献