奶牛早孕因子重组蛋白的原核表达、纯化及多克隆抗体的制备   总被引：1，自引：1，他引：0  

刘云  贾斌  石峰  李鑫  李洪涛  张永生  宁梦影  纪俊明  秦炳燕 《中国畜牧兽医》2015,42(4):877-882

本研究旨在表达、纯化奶牛早孕因子重组蛋白及制备多克隆抗体.本试验构建奶牛早孕因子重组蛋白原核表达载体pET32a-EPF,转化至大肠杆菌BL21(DE3)内进行诱导表达,SDS-PAGE切胶纯化的早孕因子重组蛋白免疫BALB/c小鼠制备多克隆抗体,并用Western blotting和ELISA对重组蛋白及抗体进行检测.结果显示,成功表达并纯化了奶牛早孕因子重组蛋白,重组蛋白分子质量约37 ku,表达量约占菌体总蛋白的48.6%,纯化后目的蛋白纯度可达93%,重组蛋白可与所制备的多克隆抗体结合,ELISA测定的抗体效价为1:12 800.结果表明,本研究成功表达并纯化了奶牛早孕因子重组蛋白,为研究奶牛早孕诊断奠定基础.  相似文献   

B型超声诊断技术在波尔山羊妊娠早期的应用研究   总被引：4，自引：0，他引：4  

严成禄  林宝智  韩旭东  陈有旺  李铁拴 《畜牧与饲料科学》2009,30(5):73-74

为了提高波尔山羊的繁殖效率,试验采用B型超声诊断技术在波尔山羊妊娠早期进行了声像学监测.对早期妊娠诊断以及怀胎数目诊断进行了研究.结果显示:在波尔山羊妊娠早期,即妊娠40 d之前,主要以直肠检查为主,腹壁检查为辅;妊娠40d以后腹壁检查时胎体清晰可见,主要以腹壁检查为主,妊娠诊断的准确率为100%,双胎诊断的准确率为100%,单胎诊断的准确率为81.8%.  相似文献   

Early pregnancy alters the metabolic responses to restricted nutrition in sheep     

Sosa C  Abecia JA  Carriquiry M  Forcada F  Martin GB  Palacín I  Meikle A 《Domestic animal endocrinology》2009,36(1):13-23

This study investigated whether a 27-day period of nutrition at half-maintenance during early pregnancy (up to Day 14) could alter maternal endocrine responses. Forty-six ewes were fed all or half of their maintenance requirements and slaughtered on Day 14 of the oestrous cycle or pregnancy. We used real time RT-PCR to study gene expression of growth hormone receptor (GHR) and leptin in adipose tissue and GHR, GHR1A and of the insulin-like growth factor I (IGF-I) in the liver. Blood profiles of metabolites and metabolic hormones were also determined. Throughout the experiment, underfed animals presented lower body weight and body condition, greater plasma concentrations of non-esterified fatty acids (NEFA), and lower plasma concentrations of leptin, compared to adequately fed animals. Undernutrition affected the patterns of gene expression in adipose and hepatic tissues, and the responses differed between pregnant and non-pregnant ewes. In adequately fed ewes, pregnancy up-regulated leptin mRNA expression in adipose tissue, a response that was impaired in underfed ewes. The hepatic expression of IGF-I mRNA was increased by pregnancy in underfed animals while no effect was observed in adequately fed ewes. It remains to be determined whether the changes in the endocrine milieu are paralleled by modifications in uterine gene expression that could alter the environment of the embryo during early pregnancy.  相似文献   

交感神经对妊娠早期小鼠子宫内膜发育的影响     

赵娇慧  陈耀星  王子旭  董玉兰  白永平  谢电 《畜牧兽医学报》2009,40(11)

为了研究交感神经对小鼠妊娠早期(妊娠第1～9天)子宫内膜发育的影响,本试验采用交感神经阻断剂6-羟多巴胺(6-OHDA,100 mg·kg~(-1)体质量)腹腔注射成年雌性昆明小白鼠,损毁外周交感神经.利用放射免疫分析法、组织学和免疫组织化学技术,检测了小鼠妊娠早期血清17β-雌二醇水平、子宫腺发育和子宫内膜细胞增殖的变化.结果显示:(1)在妊娠第1天(E1)、E5和E7 6-OHDA处理组血清中17β-雌二醇的含量分别降低了63.4%(P<0.01)、35.7%(P<0.05)和25.5%(P<0.05),而在E3和E9分别升高了79.3%(P<0.05)和156.7%(P<0.01);(2)6-OHDA处理降低了妊娠早期子宫腺的比例,与同期对照组相比分别下降了37.5%、38.6%、41.8%、61.4%和58.1%(P<0.01);(3)6-OHDA处理降低了妊娠早期子宫内膜细胞的增殖,使E1、E3、E5、E7和E9子宫内膜增殖细胞分别减少了27.2%、30.2%、40.7%、27.2%和27.7%(P<0.01).上述结果表明,交感神经对雌激素的分泌以及妊娠早期子宫组织结构的重建具有重要的调控作用,进而调节动物早期胚胎的着床和发育.  相似文献   

Surgical management of complete diaphyseal third metacarpal and metatarsal bone fractures: Clinical outcome in 10 mature horses and 11 foals     

A. S. BISCHOFBERGER  A. FÜRST  J. AUER  C. LISCHER 《Equine veterinary journal》2009,41(5):465-473

Reasons for performing study: Osteosynthesis of third metacarpal (McIII) and third metatarsal (MtIII) bone fractures in horses is a surgical challenge and complications surrounding the repair are common. Retrospective studies evaluating surgical repair, complications and outcome are necessary to increase knowledge and improve success of long bone fracture repair in the horse. Objectives: To evaluate clinical findings, surgical repair, post operative complications and outcome of 10 mature horses and 11 foals with McIII or MtIII fractures that were treated with open reduction and internal fixation (ORIF). Methods: Medical records were reviewed and follow‐up information obtained by means of radiographs and/or telephone questionnaire. Results: Survival was achieved in 62% of the horses (3 mature/10 foals). On long‐term evaluation (>6 months) 11 horses (2 mature/9 foals) were fit for their intended activity, one mature horse had a chronic low grade lameness, and one foal was lost to follow‐up because it was sold. The main fracture types were simple transverse (33.3%) or simple oblique (28.6%) and 71.4% of the fractures were open, 3 Type I (one mature/2 foals) and 12 type II (7 mature/5 foals). The preoperative assessment revealed inadequate emergency treatment in 10 horses (5 mature/5 foals; 47.6%). Survival rate of horses with open fractures was 12.5% (1/8) in mature and 85.7% (6/7) in foals. Post operative incisional infection (4 mature, 3 foals) was only managed successfully in 2 foals. Fracture instability related to inadequate fracture fixation technique occurred in 4 horses (all mature) and was always associated with unsuccessful outcome. Conclusions: Age, bodyweight and infection are strongly associated with outcome in treatment of complete McIII/MtIII fractures. Clinical relevance: Rigid fixation using plates and screws can be successful in treatment of closed or open, complete diaphyseal McIII/MtIII fractures in mature horses and foals. Instable fixation, infection and a bodyweight >320 kg are major risk factors for unsuccessful outcome.  相似文献   

分离Y精子在茸鹿性别控制中的应用研究     

赵列平  韩欢胜  赵广华  刘世明  王子富  常书权 《经济动物学报》2009,13(4):187-189,194

为研究一种新的茸鹿繁殖方法,2007～2008年对469头马鹿,79头梅花鹿进行性别控制技术研究。结果表明：性别控制冻精情期受胎率马鹿为87．21％,梅花鹿为60．76％,两者之间差异极显著（P〈0．01）,性比率（指产公仔率）马鹿为90．95％,梅花鹿为93．75％,两者之间无显著差异（P〉0．05）;性别控制冻精与普通冻精输精比较,马鹿情期受胎率之间、性比率之间和梅花鹿性比率之间都存在极显著差异（P〈0．01）,梅花鹿情期受胎率之间则无显著差异（P〉0．05）。普通冻精受胎率马鹿为94．89％、梅花鹿为61．63％,性比率马鹿为50．22％、梅花鹿为49．06％,性别控制冻精受胎率整体接近普通冻精,性比率则提高40％以上,比较分析认为对茸鹿X、Y精子分离进行性别控制试验是成功的。  相似文献   

MAGNETIC RESONANCE IMAGING FINDINGS OF DESMOPATHY OF THE COLLATERAL LIGAMENTS OF THE EQUINE DISTAL INTERPHALANGEAL JOINT     

Santiago D. Gutierrez-Nibeyro  Nathaniel A. White II    Natasha M. Werpy  Laurie Tyrrell  Kent A. Allen  Kenneth E. Sullins  Richard D. Mitchell 《Veterinary radiology & ultrasound》2009,50(1):21-31

We report the use of a low-field magnetic resonance (MR) imaging system for the detection of desmopathy of the collateral ligament of the distal interphalangeal joint and the long-term outcome. Twenty horses were studied and their medical records and MR images were reviewed retrospectively. Long-term follow-up information was obtained by telephonic questionnaires of owners, trainers, or referring veterinarians. Desmopathy of the medial collateral ligament (80%) and enthesopathy of the affected collateral ligament (80%) were common MR imaging features. Treatment consisted of stall rest followed by a rehabilitation period. Additional treatments included shoeing, extracorporeal shock wave therapy, application of a half limb or foot cast, and medication of the distal interphalangeal joint. Twelve (60%) horses returned to their previous level of exercise and maintained their previous level, whereas eight horses had a poor outcome. Low-field MR imaging in the standing patient can be used to detect collateral ligament desmopathy of the distal interphalangeal joint without a need for general anesthesia.  相似文献   

羊超声波早期妊娠诊断技术的研究     

刁显辉  孟详人  何海娟  刘玉峰 《黑龙江农业科学》2011,(5):55-56

结合生产实践,采用超声波仪对羊进行了早期妊娠诊断技术的应用研究试验。结果表明：发情配种后35 d以上的母羊可以采用超声波仪确定其是否怀孕,准确率接近于100%。这种方法不仅可以及时将妊娠母羊隔离饲养,而且能够对未妊娠母羊及时补配或淘汰,降低饲养成本。  相似文献   

妊娠后期胎儿宫内生长受限对出生后羔羊内脏器官的影响     

高峰  刘迎春 《中国农业科学》2012,45(15):3130-3136

【目的】研究妊娠后期胎儿宫内生长受限对出生后羔羊内脏器官的影响。【方法】选择健康的蒙古绵    羊45只（经同期发情且同期受孕）,妊娠（90±1） d分为自由采食组（CG）、0.33 MJ ME•kgw-0.75•d-1（RG1）以及0.175 MJ ME•kgw-0.75•d-1（RG2）3个营养水平处理组,进行不同营养水平的限饲饲养。各组分别于羔羊初生及28周龄时选择4只屠宰,取出内脏。【结果】妊娠后期不同营养水平限饲母羊,严重限制了RG1（P＜0.05）、RG2（P＜0.01）组羔羊的平均初生体重：①初生时,RG1组羔羊仅肺脏重和肺脏组织总DNA含量显著低于CG组（P＜0.05）,脾脏重及脾脏组织总DNA含量极显著低于CG组（P＜0.01）;而 RG2组羔羊肺脏和脾脏均显著低于CG组（P＜0.01）,心脏、肝脏、肾脏、总胃及小肠重均显著低于CG组（P＜0.05）;其中,心脏和肾脏总DNA含量、肺脏总DNA含量和蛋白与DNA含量比、肝脏组织蛋白和DNA含量比均显著低于CG组（P＜0.05）。②28周龄时,RG1组胎儿期生长受限的肺脏、脾脏重及其总DNA含量与对照组差异不显著（P＞0.05）;除心脏外,RG2组胎儿期生长受限的肝脏、肺脏及脾脏重出生后经过28周的生长与CG组差异不显著（P＞0.05）;RG2组胎儿期降低的肾脏、脾脏细胞总DNA含量,肝脏细胞蛋白和DNA比,在试验结束羔羊28周龄时与CG组差异不显著（P＞0.05）,并且肺脏细胞蛋白和DNA比显著高于CG组（P＜0.05）,而心脏和肺脏组织总DNA含量仍显著低于CG组（P＜0.05）。【结论】妊娠后期胎儿宫内生长受限通过对其胎儿内脏器官细胞增殖与增肥不同程度的影响而改变了出生后羔羊内脏器官的生长发育轨迹。  相似文献   

饲粮添加脯氨酸对妊娠环江香猪体成分的影响     

张婷  查伟  唐子龙  孔祥峰 《动物营养学报》2017,29(2)

本试验旨在研究饲粮添加脯氨酸对妊娠环江香猪体成分的影响。选取32头妊娠15 d的环江香猪,随机分为2组,每组8个重复,每个重复2头猪。2组试验猪分别饲喂在基础饲粮中添加0.77%L-丙氨酸(等氮对照组)和1.00%L-脯氨酸(脯氨酸组)的饲粮。分别于妊娠第45和70天时,每个重复选择1头母猪屠宰,测定其体成分。结果表明:与等氮对照组相比,妊娠第45天时脯氨酸组环江香猪的脂肪率显著降低(P0.05),皮肤率呈增加趋势(0.05≤P0.10);妊娠第70天时脯氨酸组的脂肪率显著增加(P0.05),皮肤率呈降低趋势(0.05≤P0.10),背最长肌中脯氨酸的含量显著增加(P0.05),精氨酸和丙氨酸的含量呈增加趋势(0.05≤P0.10)。与妊娠第45天时相比,妊娠第70天时脯氨酸组的脂肪率显著增加(P0.05),瘦肉率与皮肤率显著降低(P0.05)。由此可见,饲粮添加脯氨酸可增加妊娠环江香猪机体脂肪和精氨酸的沉积量,妊娠中期的机体脂肪沉积量比妊娠前期有所增加,从而有利于胎儿的生长发育。  相似文献