一株仔猪肠道益生菌抗TGEV作用初探     

修晶辉  李伟娜  尹鑫  刘宁  魏萍 《黑龙江畜牧兽医》2012,(3):17-20,163

为了分离并筛选出1株抗传染性胃肠炎病毒(TGEV)的益生菌菌株,研究采用形态观察、生化反应和16S rRNA鉴定等试验,并运用TCID50和MTT比色方法,在PK-15细胞水平上分别检测益生菌活菌、灭活菌体、菌体碎片及培养上清液在不同作用时间上对TGEV感染力和抑制率的影响。结果表明:从健康仔猪肠道内容物分离、鉴定出1株益生菌,属于德氏乳杆菌德氏亚种(暂命名为L1菌株),此菌株的活菌、灭活菌体、菌体碎片及培养上清液均有降低TGEV感染力和活性的作用,其中活菌降低TGEV感染力及对TGEV的抑制效果最明显,灭活菌体和培养上清液次之,菌体碎片最差。  相似文献   

饲粮添加益生菌与酶制剂对黄羽肉鸡生长性能的影响及相关机理   总被引：3，自引：0，他引：3  

林谦  戴求仲  宾石玉  蒋桂韬  张建华  张旭  王照群  王向荣 《动物营养学报》2012,(10):1955-1965

为研究益生菌与酶制剂对黄羽肉鸡生长性能、养分代谢率和肠黏膜形态的影响,试验选择5日龄体重相近的健康雌性黄羽肉鸡675羽,随机分成5个处理,每个处理5个重复,每个重复27羽,空白Ⅰ组饲喂基础饲粮,Ⅱ、Ⅲ、Ⅳ、Ⅴ组分别饲喂在基础饲粮中添加25 g/t益生菌制剂、150 g/t复合酶制剂、25 g/t益生菌制剂+150 g/t复合酶制剂、300 g/t抗敌素的试验饲粮。饲养试验至53日龄结束。结果表明:饲粮中组合添加益生菌与酶制剂获得了最高的全期平均日增重,且与空白Ⅰ组及抗生素Ⅴ组相比分别提高了4.22%(P<0.05)和2.12%(P>0.05);真代谢能以组合添加Ⅳ组最高,且与空白Ⅰ组及抗生素Ⅴ组相比分别提高了11.29%(P<0.01)和6.89%(P<0.05),酶制剂Ⅲ组粗纤维真代谢率极显著高于Ⅰ、Ⅱ、Ⅴ组(P<0.01),显著高于组合添加Ⅳ组(P<0.05);抗生素、益生菌与酶制剂及两者组合使用都不同程度地促进了十二指肠、空肠和回肠绒毛形态结构的发育。总体上说,益生菌与酶制剂联合使用表现出了一定优势,且具有替代某些抗生素饲料添加剂使用的潜能。  相似文献   

复合酶制剂对凡纳滨对虾生长性能、血浆生化指标和免疫性能的影响     

申亮  于志文  韩佳乐  何立彬  郭冉 《河北渔业》2022,(2):16-20,46

为研究复合酶制剂对凡纳滨对虾(Litopenaeus vannamei)生长性能、血淋巴生化指标和免疫性能的影响,选用初始体重为(0.40±0.02)g的虾苗240尾,随机分为两组,对照组投喂基础饲料,试验组饲料中添加0.04％复合酶制剂,试验为期10周.结果 显示:与对照组相比,试验组凡纳滨对虾生长性能无显著变化(P...  相似文献   

Effects of using a chicken-origin competitive exclusion culture and probiotic cultures on reducing Salmonella in broilers     

《The Journal of Applied Poultry Research》2009,18(1):23-29

Three commercial products, a partially defined chicken-origin competitive exclusion culture (Aviguard, 0.50 mL/chick) and 2 single-organism probiotic cultures (Saccharomyces cerevisiae, Levucell SC, 1 g/kg of feed, and Pediococcus acidilactici, Bactocell, 100 mg/kg of feed) were evaluated for their ability to reduce Salmonella in broilers and their effects on production performance. It was found that all the treatments significantly (P < 0.05) reduced Salmonella concentrations, as compared with the control, on the chicken, in the ceca, and on the chicken carcass. In addition, it was found that these treatments had no adverse effect on any of the production parameters that were measured. Finally, this study showed the importance of using these preharvest treatments as part of an integrated program to control Salmonella at the broiler farm.  相似文献   

Feeding beneficial bacteria: A natural solution for increasing efficiency and decreasing pathogens in animal agriculture     

《The Journal of Applied Poultry Research》2009,18(2):367-378

Consumer demand for natural and organic foods has risen steadily. Correspondingly, the demand for technologies to enhance animal performance through natural and organic solutions continues to increase. One such technology receiving considerable attention is the feedstuff application of live, beneficial bacteria, commonly called probiotics, but properly referred to as direct-fed microbials (DFM). The use of DFM in animal agriculture has grown continually over the last decade, yet the commercial success of DFM in poultry production to date has been limited. Overviewed here are the history, commercial applications, and research trials of DFM technology in animal agriculture as a means to gain some foresight into the future commercial use of DFM in poultry. We discuss successful probiotic trials in animal agriculture and emphasize those in poultry production that demonstrate 1) improved BW gain, 2) decreased morbidity, 3) decreased incidence of bird and human pathogens, and 4) increased economic profitability. Last, we present current and future obstacles and basic microbiological concepts that are essential for functional probiotic technologies.  相似文献   

不同酶制剂在生长猪小麦型日粮中应用效果的研究   总被引：2，自引：2，他引：0  

王前光  刘秋  高惠林  贺建华 《中国畜牧兽医》2009,36(10):16-20

本研究选用4种不同的酶制剂（E1、E2、欧蒂酶(OT)、木聚糖酶）,以小麦基础日粮为试验材料,探讨不同酶制剂在生长猪小麦日粮中的应用效果。采用单因素试验设计,选用112头体重19.34 kg左右的二元（长×大）杂交猪,随机分为7个处理组,每组4个重复,每个重复4头猪,公母各半。试验期8周,分为0～2周、2～4周和4～8周3个阶段,饲喂日粮分别为玉米型日粮、小麦基础日粮、小麦基础日粮+不同酶制剂（E1,E2,欧蒂酶,木聚糖酶）和低能小麦型日粮+欧蒂酶,考察酶制剂对生长猪生产性能的影响;同时,另选6头体重在20 kg左右的二元（长×大）杂交阉公猪于回肠末端安装T型瘘管,采用6×6拉丁方试验设计进行消化试验,利用内源指示剂法(AIA)测定酶制剂对营养物质的表观回肠和粪消化率。试验结果表明,与小麦基础组相比,小麦日粮中添加酶制剂能提高生长猪的平均日增重,2～4周时有提高趋势（P=0.10）;与小麦对照组相比较,E1组全期平均日增重差异不显著（P>0.05）, 木聚糖酶组和E2组差异显著（P<0.05）,欧蒂酶组差异极显著（P<0.01）。小麦日粮中添加酶制剂均能显著降低生长猪全期料重比（P<0.05）;同时,酶制剂能显著降低生长猪4～8周及全期的腹泻率（P<0.05）,E2组、欧蒂酶组、木聚糖酶组达极显著水平（P<0.01）;消化试验结果表明,酶制剂有提高日粮中蛋白质和能量的粪表观消化率,但差异不显著（P>0.05）;酶制剂的添加有提高能量的回肠表观消化率的趋势（P=0.10）;E2、欧蒂酶、木聚糖酶的添加能显著提高粗蛋白质的回肠表观消化率（P<0.05）,E1组差异不显著（P>0.05）。  相似文献   

饲用益生素在畜禽健康养殖中的应用   总被引：2，自引：0，他引：2  

吕玉丽  徐子伟  吴建良  李永明  邓波 《浙江农业学报》2012,24(1):180-186

益生素是一种新型无毒、绿色的微生物类饲料添加剂,具有调节动物机体微生态平衡、预防疾病、提高饲料转化率、促进动物健康和保护生态环境等多方面功能.因其在使用过程中无毒副作用、无药物残留、不产生耐药性,而成为抗生素的理想替代品,广泛应用于畜禽生产中.文章综述了饲用益生素的起源、种类及作用机理,探讨了益生素在畜禽健康养殖中的应用前景及发展趋势.  相似文献   

Influence of Zinc Bacitracin and Bacillus licheniformis on Microbial Intestinal Functions in Weaned Piglets     

Collinder E  Cardona ME  Berge GN  Norin E  Stern S  Midtvedt T 《Veterinary research communications》2003,27(7):513-526

The influence of zinc bacitracin (ZB) and of Bacillus licheniformis on host microbial-related functions in young piglets was investigated by applying the concept of microflora-associated characteristics. Six biochemical parameters were determined before and after weaning in faecal samples from piglets in four litters having access to a diet containing ZB, to a diet containing B. licheniformis, to a diet with both additives, or to a diet with no additives, from 3 weeks of age. Statistically significant differences were found in three of the intestinal functions investigated: formation of short-chain fatty acids (at 7 and 10 weeks of age), degradation of mucin (at 7 and 10 weeks of age) and conversion of bilirubin to urobilins (at 7 weeks of age). We also found age-dependent influences on the formation of short-chain fatty acids, on conversion of cholesterol to coprostanol and on conversion of bilirubin to urobilins. We conclude that a functional approach is appropriate for measuring exogenous influence(s) on the microbial intestinal metabolisms in weaned piglets.  相似文献   

非淀粉多糖酶制剂对雏鸡生长、免疫功能和肠道微生物区系的影响   总被引：12，自引：0，他引：12  

高峰  江芸  周光宏  韩正康 《中国兽医学报》2004,24(5):501-503

2次重复试验研究了小麦米糠基础日粮添加非淀粉多糖酶制剂对雏鸡生长、免疫功能和肠道微生物区系的影响。每次试验 4 8只 7日龄鸡随机分为 2组 ,即基础日粮组、基础日粮添加 0 .1%酶组。结果表明 ,酶制剂能显著提高雏鸡增重和饲料转化率 (P<0 .0 5 ) ,使雏鸡的免疫器官相对重量增加 (P<0 .0 5 ) ,T淋巴细胞对 PHA的反应性、NK细胞活力、血清新城疫疫苗抗体效价显著提高 (P<0 .0 5 )。盲肠乳酸杆菌数略有增加 ,大肠杆菌略有下降 ,但差异不显著(P>0 .0 5 )  相似文献   

载菌蒙托石改善鲫养殖水质和生长性能的研究   总被引：1，自引：0，他引：1  

温军辉  王彦波  韩剑众 《水产科学》2008,27(11)

以附载益生菌-沼泽红假单胞菌的蒙托石为研究对象,通过对鲫养殖水质指标和生长性能的测定,研究了其作用效果.试验分为1个对照组和3个试验组(T1、T2、T3分别添加一定量的沼泽红假单胞菌、蒙托石和载菌蒙托石).试验结果表明,与对照组相比,试验组T3显著降低了(P < 0.01)养殖水体后期氨氮、亚硝酸盐氮、硝酸盐氮、化学需氧量和硫化物的含量,提高了鲫的质量日增长和质量相对增长率(P < 0.01),改善效果优于T1和T2.试验组T1和T2在生长指标上与对照组比较,虽有不同程度的提高,但差异不显著(P > 0.01).  相似文献