浅谈环境因子对绒山羊产绒性能的影响     

穆秀梅  马启军  王树华  淡江华 《畜牧兽医杂志》2009,28(1):47-49

文章综述了主要环境因子光照、环境温度、气候、营养等对绒山羊产绒性能的影响,为改善绒山羊生产的环境条件,研究新的绒山羊饲养工艺提供理论基础和现实方法。  相似文献   

内蒙古绒山羊皮肤干细胞定位的初步研究   总被引：1，自引：0，他引：1  

席海燕  郑琰  周欢敏 《湖北农业科学》2009,48(2)

为了确定干细胞在内蒙古绒山羊初级毛囊、次级毛囊及表皮中的位置,通过对绒山羊颈部静脉注射BrdU,每周取皮肤样本,用4%多聚甲醛固定,常规石蜡包埋,切片,免疫组织化学检测.结果发现,免疫组织化学检测在前10周内BrdU结果呈阳性,说明皮肤中存在干细胞,BrdU可用于大动物短时期的体内标记;BrdU用于大动物标记时在绒山羊体内的存留时间大约为10周;初步判断初级毛囊和次级毛囊及表皮中均存在干细胞,而且干细胞的niche可能就位于毛乳头处;用Ⅳ型胶原粘附培养初级和次级毛囊的毛乳头,其呈圆形或多边形,核大,核仁明显,细胞器少,细胞呈克隆生长,慢周期性,具有无限的增殖能力.经流式细胞术检测,初步证明为毛乳头处存在毛囊干细胞.  相似文献   

辽宁绒山羊Cytb基因的克隆和序列测定     

王春强  马巍  马宁 《安徽农业科学》2009,35(19)

[目的]分析测定辽宁绒山羊Cytb基因序列,为我国山羊品种系统分类的进一步研究提供有益的探索。[方法]根据GenBank发表的山羊的m tDNA的核酸序列,结合软件设计一对引物,对辽宁绒山羊Cytb基因进行了克隆测序。[结果]序列长度为1 140 bp,A、T、G、C 4种核苷酸的平均含量分别为31.8%、26.8%、13.2%、28.3%,其中,A+T含量(58.6%)明显高于G+C含量(41.5%)。[结论]在系统发育研究中,Cytb基因是一个十分有效的标记。  相似文献   

辽宁绒山羊2品系皮肤毛囊结构及其活性变化规律的比较   总被引：4，自引：0，他引：4  

赵艳丽  张世伟  姜怀志 《吉林农业大学学报》2009,31(6)

对辽宁绒山羊常年长绒型和季节长绒型2品系进行皮肤采样,利用Sacpic染色法制备组织切片,在显微镜下观察其皮肤毛囊结构及其活性变化。结果发现:2品系毛囊结构一致。毛囊各性状呈周期性变化,初、次级毛囊深度值均在8月份最高,次年2月份下降到最低。季节型次级毛囊宽度值在4月份最低,而常年型的在2月份最低。常年型次级毛囊密度和活性均高于季节型,尤其是2~4月份差异明显。次级毛囊活性在8月份达到最高,12月份毛囊进入退行期,2~4月份进入休止期,4~8月份毛囊开始重建。常年型S/P值稍高于季节型,但只是在2月份二者差异明显。  相似文献   

河北本地绒山羊的杂交选育研究     

王振来  赵德明 《安徽农业科学》2009,37(20):9489-9490

［目的］改进河北省本地绒山羊的生产性能,从而促进羊绒产业的发展。［方法］以辽宁绒山羊为父本,以本地绒山羊为母本,级进杂交到F2代,进行横交固定。测定选育后绒山羊的体尺指标、体重、产绒量,评价羊绒品质,并统计产羔率和净绒率。［结果］与本地绒山羊相比,选育后的绒山羊体格健壮,体尺指标良好,体重和产绒量显著增加,羊绒显著变长,产羔率和净绒率得到了进一步提高。［结论］选育后的绒山羊优质高产,生产性能显著提高。  相似文献   

内蒙古绒山羊CRABP I基因的克隆及表达     

李华  苏立宁  刘东军  李雪峰  旭日干 《中国农业科学》2012,45(13):2743-2750

【目的】克隆内蒙古绒山羊视黄酸结合蛋白Ⅰ（cellular retinoic acid binding proteinⅠ,CRABPⅠ）基因的cDNA,进行蛋白结构的预测和表达分析,为绒山羊毛和绒形成的分子机理奠定基础。【方法】利用RT-PCR方法克隆内蒙古绒山羊CRABPⅠ基因序列,利用生物信息学方法预测其蛋白结构,采用实时荧光定量PCR探讨该基因在绒山羊4个胎儿日龄皮肤中的表达。【结果】内蒙古绒山羊CRABP I基因cDNA长679 bp（JN936490）,其开放阅读框为414 bp,氨基酸序列与其它物种相比具有较高的序列相似性。CRABP Ⅰ蛋白无明显的信号肽和跨膜区域,不存在N糖基化位点和O糖基化位点;蛋白二级结构主要由α 螺旋、β折叠和少量的转角及无规则卷曲构成。胎儿皮肤中CRABPⅠ基因在 90 d 的表达量明显高于100、120和130 d（P<0.05）。【结论】绒山羊的CRABPⅠ基因cDNA中的开放阅读框（open reading frame,ORF）在不同物种间较为保守,但种属特异性集中表现在第33和123氨基酸残基处,该基因在胎儿期的90 d表达量最大。  相似文献   

Genome Array on Differentially Expressed Genes of Skin Tissue in Cashmere Goat at Early Anagen of Cashmere Growth Cycle Using DNA Microarray          下载免费PDF全文

DI Jiang XU Xin-ming Lazate Ainiwaer ZHANG Yan-hua TIAN Ke-chuan YU Li-juan WU Wei-wei Hanikezi Tulafu FU Xue-feng Marzeya Yasen 《农业科学学报》2014,13(10):2243-2252

In order to study the molecular mechanism involved in cashmere regeneration, this study investigated the gene expression profile of skin tissue at various stages of the cashmere growth cycle and screen differentially expressed genes at proangen in 10 cashmere goats at 2 years of age using agilent sheep oligo microarray. Significance analysis of microarray （SAM） methods was used to identify the differentially expressed genes, Hierarchical clustering was performed to clarify these genes in association with different cashmere growth stages, and GO （Gene ontology） and the pathway analyses were con-ducted by a free web-based Molecular Annotation System3.0 （MAS 3.0）. Approximately 10200 probe sets were detected in skin tissue of 2-yr-old cashmere goat. After SAM analysis of the microarray data, totally 417 genes were shown to be differentially expressed at different cashmere growth stages, and 24 genes are significantly up-regulated （21） or down-regulated （3） at proangen concurrently compared to angen and telogen. Hierarchical clustering analysis clearly distinguished the differentially expressed genes of each stage. GO analysis indicated that these altered genes at proangen were predominantly involved in collagen fibril organization, integrin-mediated signaling pathway, cell-matrix adhesion, cell adhesion, transforming growth factor-β （TGF-β） receptor signaling pathway, regulation of cell growth. Kyoto encyclopedia of genes and genomes （KEGG） analysis showed that the significant pathways involved mainly included focal adhesion and extracellular matrixc （ECM）-receptor interaction. Some important genes involved in these biological processes, such as COL1A1, COL1A2, COL3A1, SPARC, CYR61 and CTGF, were related to tissue remolding and repairing and detected by more than one probe with similar expression trends at different stages of cashmere growth cycle. The different expression of these genes may contribute to understanding the molecular mechanism of cashmere regeneration.  相似文献   

Effects of Photoperiod on Stem Cells Activation in Cashmere Goat Hair Follicles   总被引：1，自引：0，他引：1  

LIU Bin  GUO Jun  Wudubala  MA Yue-jun  XIN Lei-yong  ZHAO Cun-fa  LI Yu-rong  YIN Jun 《中国畜牧兽医》2017,44(4):994-1000

In order to extend the anagen of cashmere goat hair follicles and increase the production of cashmere,this study was performed with artificially shorten the daylight time among Arbas White cashmere goats. Skin tissue sections from cashmere goats were collected to compare the morphologic changes between artificial daylight and natural daylight,and immunohistochemical method was used to study the hair follicle cell proliferation and important protein expression in related signaling pathways. The results showed that strong cell proliferation occurred in cashmere goat hair follicle cells during artificial daylight,plenty of cytokeratin 15 (K15) positive signals were distributed in the outer root sheath,β-catenin protein was actively expressed in hair matrix and root sheath, indicating that the hair follicles were in the anagen growth phase;Meanwhile,cashmere goat hair follicles under natural daylight were in telogen with weak signals. Above all prove that short photoperiod played an important role in promoting hair follicle growth,the artificial short photoperiod could change hair follicle growth cycle and make hair follicles earlier enter to the anagen growth phase,causing a variety of typical gene expressions during hair follicle growth.  相似文献   

内蒙古绒山羊皮肤KRTAPs表达谱与日照时长的相关性     

赵濛  奈日乐  谢遇春 《西北农林科技大学学报(自然科学版)》2017,45(9):17-24

【目的】研究绒山羊皮肤部分角蛋白关联蛋白(keratin-associated protein,KRTAPs)基因的表达谱及其与全年日照变化规律间的相关性。【方法】以3只周岁阿尔巴斯型内蒙古绒山羊体侧皮肤为样本,进行高通量转录组测序(RNA-Seq),对获得的部分KRTAPs基因一年内不同月份间的转录本进行表达丰度分析,并结合当地全年日照变化规律与RT-PCR试验方法,验证KRTAP24-1、KRTAP3-1、KRTAP8-1在关键节点月份的表达谱,确定全年皮肤KRTAPs表达丰度变化与日照变化规律间的相关性。【结果】Illumina solexa高通量转录组测序表明,内蒙古绒山羊全年皮肤部分KRTAPs基因的表达具有规律性差异。RT-PCR试验验证表明,部分基因表达谱与测序表达丰度具有一致性,并且基因差异变化规律与全年日照时长的变化具有相关性。【结论】全年日照时长由长变短(夏-冬)时皮肤KRTAPs表达丰度上调,日照时长由短变长(冬-夏)时皮肤KRTAPs表达丰度下调。  相似文献   

半胱胺对绒山羊瘤胃及盲肠内环境的影响     

徐军  于长江  孙运刚  刘皙洁 《中国饲料》2010,(7)

本试验选用4头6月龄、平均体重为16kg左右、安装永久性瘤胃瘘管和盲肠瘘管的辽宁绒山羊,平均分为半胱胺组和对照组,观察辽宁绒山羊瘤胃及盲肠内环境的变化。结果表明:半胱胺组瘤胃pH较对照组差异不显著(P0.05)。半胱胺组瘤胃NH3-N浓度、瘤胃蛋白浓度较对照组差异显著(P0.05);盲肠pH、蛋白浓度半胱胺组较对照组均差异不显著(P0.05)。本试验结果提示,添加半胱胺后可改变瘤胃内环境,有利于瘤胃微生物蛋白的合成。  相似文献