全文获取类型
收费全文 | 3901篇 |
免费 | 258篇 |
国内免费 | 293篇 |
专业分类
林业 | 217篇 |
农学 | 249篇 |
基础科学 | 94篇 |
847篇 | |
综合类 | 1449篇 |
农作物 | 166篇 |
水产渔业 | 408篇 |
畜牧兽医 | 774篇 |
园艺 | 109篇 |
植物保护 | 139篇 |
出版年
2024年 | 22篇 |
2023年 | 60篇 |
2022年 | 105篇 |
2021年 | 133篇 |
2020年 | 155篇 |
2019年 | 168篇 |
2018年 | 119篇 |
2017年 | 174篇 |
2016年 | 196篇 |
2015年 | 164篇 |
2014年 | 180篇 |
2013年 | 218篇 |
2012年 | 251篇 |
2011年 | 236篇 |
2010年 | 230篇 |
2009年 | 256篇 |
2008年 | 177篇 |
2007年 | 178篇 |
2006年 | 184篇 |
2005年 | 147篇 |
2004年 | 134篇 |
2003年 | 117篇 |
2002年 | 116篇 |
2001年 | 114篇 |
2000年 | 107篇 |
1999年 | 79篇 |
1998年 | 56篇 |
1997年 | 80篇 |
1996年 | 45篇 |
1995年 | 37篇 |
1994年 | 51篇 |
1993年 | 31篇 |
1992年 | 30篇 |
1991年 | 18篇 |
1990年 | 18篇 |
1989年 | 21篇 |
1988年 | 16篇 |
1987年 | 9篇 |
1986年 | 2篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 1篇 |
1982年 | 2篇 |
1980年 | 1篇 |
1979年 | 2篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1962年 | 1篇 |
排序方式: 共有4452条查询结果,搜索用时 15 毫秒
71.
在南朝鲜于1999年的2月至12月间,根据有关文献,调查问卷和对原麝的种类,粪便,及其在有雪和无雪的季节里的野外踪迹的调查,作者概述了50年中原麝的数量和分布区的变化。结果表明:从50年代至1999间,原麝(Moschus moschiferus parvipes)的分布区呈明显缩减之势。由于在原麝分布区中大规模地进行公路及林道的建设,导致了其生境片断化和丢失。而且牟取利润的非法猎捕也是造成原麝的种群数量和分布区缩减的主要原因,在高海拔地区的林道也常被盗猎者所利用。为保护韩国原麝资源,急待严格禁止非法猎捕行为。原麝生活习性方面的研究,为合理的保护和管理原麝提供了重要的基础信息。图1参9。 相似文献
72.
73.
White-tailed deer (Ododcoileus virginiana) can substantially affect the structure and species composition of a forest. The tolerance of a forest community to browsing may vary by type as a result of varying biotic and abiotic factors of the environment. To date, no studies have compared the effects of browsing among forest communities within a physiographic region. We investigated the effects of browsing on vegetation structure and woody seedling composition in three forest types (oak–hickory, Virginia pine–eastern red cedar, bottomland hardwood) in Manassas National Battlefield Park (MNBP), Virginia, USA. We compared forb cover, vertical plant cover (0–1.5 m tall), and survival of tagged seedlings in 10 exclosed (2 m × 6 m) and 10 unexclosed plots in each forest type during a 5-year period. No differential effects of browsing were found among forest types. In all forest types, deer (67 deer/km2) suppressed forb and vertical plant cover to levels less than would be expected in the absence of deer. Seedling survival rates of most species were significantly reduced by browsing. By the 4th year of the study, box elder (Acer negundo), hickory (Carya spp.), and red maple (Acer rubrum) had been eliminated from unexclosed plots, and red and white oaks (Quercus spp.) dramatically reduced. Ash (Fraxinus spp.), black cherry (Prunus serotina), and hackberry (Celtis occidentalis), although significantly impacted, remained the most abundant species throughout the study. These findings suggest that white-tailed deer may be modifying the structure of the forest interior to the extent that it adversely affects wildlife species dependent on a dense understory to thrive. We predict that the future composition of forests in MNBP will shift towards stands with fewer species and a greater dominance of ash, black cherry, and hackberry, particularly in the oak–hickory and bottomland hardwood forests, where the majority of current dominants are most affected. 相似文献
74.
Dormancy induction in container-grown Abies seedlings: Effects of environmental cues and seedling age 总被引:1,自引:0,他引:1
Twelve-week-old container-grown seedlings of noble fir (Abies procera Rehd.) and Shasta red fir (A. magnifica A. Murr. var. shastensis Lemm.), both high-elevation species, were grown under controlled environments in a study of induction of terminal-bud dormancy. Eight treatment combinations of long (15 h) or short (11 h) photoperiods, warm (25°/20°C) or cool (18°/12°C) thermoperiods, and dry (–1.2 MPa) or wet (–0.6 MPa) moisture regimes were imposed upon seedlings for 12 weeks. Treatment factors significantly affecting the induction of dormancy in terminal buds of seedlings were identified over time. The results suggest that seedlings responded dynamically to dormancy cues. The warm/dry and cool/wet combinations induced dormancy in the first 2 weeks in noble fir and in the first week in Shasta red fir. Short-day/warm and long-day/cool combinations enhanced dormancy induction in weeks 3 to 4 in noble fir and in weeks 1 to 7 in Shasta red fir. Short days and cool thermoperiods independently hastened dormancy induction in noble fir in weeks 5 through 12. 相似文献
75.
Anthony S. Davis Douglass F. Jacobs Kevyn E. Wightman Zonda K. D. Birge 《New Forests》2006,31(2):293-303
Bareroot hardwood seedling production involves intensive soil management. To increase soil organic matter (OM), nurseries
commonly grow a cover crop for 1 year after every 1–2 year of seedling production. Raising soil OM levels can also be achieved
through addition of soil amendments. We studied the influence of chicken manure (CM) and composted leaf, tree, and lawn trimmings
(Cp) on soil properties and morphology of green ash (Fraxinus pennsylvanica Marsh.) and northern red oak (Quercus rubra L.) seedlings. CM was applied at 725, 1450, or 2900 kg ha−1 (CM725, CM1450, and CM2900, respectively) and Cp was applied at 200 m3 ha−1. Addition of CM and Cp significantly raised soil OM levels and altered soil chemical properties compared to the control (Ctrl).
Root-collar diameter increased with addition of CM1450, CM2900, or Cp compared to CM725 or Ctrl plots for northern red oak,
but was largest in soils amended with CM2900 for green ash. Conversely, height was greatest with addition of CM725 for northern
red oak, but green ash seedlings were shorter in Ctrl plots than in all amendments except for CM725. Root volume of green
ash and northern red oak seedlings was positively influenced by addition of CM or Cp. Seedling responses to nursery soil amendments
vary with different forms and amounts of OM. Benefits to seedling growth through application of appropriate materials in the
proper balance can improve seedling morphological quality and positively influence soil chemical properties. 相似文献
76.
本试验旨在研究不同断母乳日龄对犊牛营养物质消化和血清生化指标影响。试验选用新生红安格斯与西门塔尔杂交肉犊牛60头,根据日龄和体重随机分成5个处理,每个处理12头犊牛,公母各占1/2,试验组分别在28、42、56和70日龄进行断母乳(分别为28A、42B、56C、70D组),随后人工哺乳代乳品至90日龄;对照组( CK组)随母哺乳至150日龄。试验时间为出生至150日龄,期间测定体重(0、70、90、150日龄)和血清生化指标(70、90、150日龄)的变化。每个处理选取6头犊牛分别在断奶前(85~90日龄)和断奶后(145~150日龄)进行2期消化代谢试验。每期6 d,其中2 d预试期,4 d正试期。结果显示:1)试验全期体重各组间无显著差异(P>0.05)。2)断奶前各组犊牛营养物质消化率差异不显著(P>0.05),断奶后28A、42B组犊牛干物质消化率显著高于56C、70D组( P<0.05),有机物消化率显著高于70D组( P<0.05),其中42B组消化率略高。3)150日龄试验结束时,28A和42B组血清葡萄糖、总蛋白、甘油三酯、尿素氮、胆固醇、结合珠蛋白、白细胞介素-2、胰岛素样生长因子-1、免疫球蛋白M含量与CK组相比差异不显著( P>0.05),血清免疫球蛋白G、免疫球蛋白A含量分别显著低于、高于CK组( P<0.05),其中42B组略高。由此可见,红安格斯与西门塔尔杂交肉犊牛的断母乳时间以42日龄较为适宜。 相似文献
77.
Patrizia CASAGRANDE PROIETTI Valentina STEFANETTI Doreene Rose HYATT Maria Luisa MARENZONI Stefano CAPOMACCIO Mauro COLETTI Annalisa BIETTA Maria Pia FRANCIOSINI Fabrizio PASSAMONTI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(8):945-951
Biofilm-forming ability is increasingly being recognized as an important virulence factor in several Staphylococcus species. This study evaluated the biofilm-forming ability of sixty canine derived clinical isolates of S. pseudintermedius, using three phenotypic methods, microtiter plate test (MtP), Congo red agar method (CRA) and tube adherence test, and the presence and impact of biofilm-associated genes (icaA and icaD). The results showed that icaA and icaD genes were detected concomitantly in 55 (91.7%) of 60 isolates. A majority (88.3%) of the strains screened had matching results by the tube adherence test, MtP and PCR analysis. Better agreement (95%) was found between the PCR-based analysis and the CRA. Results of the icaA and icaD gene PCRs showed good agreement with CRA results, with a kappa of 0.7. Comparing the phenotypic methods,
the statistical analysis showed that the agreement among the phenotypical tests using categorical data was generally good. Considering two classes (biofilm producer and biofilm non-producer), the percentage of matching results between the CRA method and the tube adherence test and between the CRA method and the MtP was 93.3%. A concordance of 100% was revealed between the MtP and the tube adherence test. The results indicate a high prevalence of the ica genes within S. pseudintermedius isolates, and their presence is associated with in vitro formation of a biofilm. A combination of phenotypic and genotypic tests is recommended for investigating biofilm formation in S. pseudintermedius. 相似文献
78.
Yojiro YANAGAWA Yukiko MATSUURA Masatsugu SUZUKI Shin-ichi SAGA Hideto OKUYAMA Daisuke FUKUI Gen BANDO Masashi NAGANO Seiji KATAGIRI Yoshiyuki TAKAHASHI Toshio TSUBOTA 《The Journal of reproduction and development》2015,61(1):61-66
Generally, sika deer conceive a single fetus, but approximately 80% of pregnant females have two corpora lutea (CLs). The function of the accessory CL (ACL) is unknown; moreover, the process of ACL formation is unclear, and understanding this is necessary to know its role. To elucidate the process of ACL formation, the ovarian dynamics of six adult Hokkaido sika deer females were examined ultrasonographically together with peripheral estradiol-17β and progesterone concentrations. ACLs formed in three females that conceived at the first estrus of the breeding season, but not in those females that conceived at the second estrus. After copulation, postconception ovulation of the dominant follicle of the first wave is induced by an increase in estradiol-17β, which leads to formation of an ACL. A relatively low concentration of progesterone after the first estrus of the breeding season is considered to be responsible for the increase in estradiol-17β after copulation. 相似文献
79.
Investigation of biofilm production and icaA and icaD genes in Staphylococcus aureus isolated from heifers and cows with mastitis
下载免费PDF全文
![点击此处可从《Animal Science Journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Lívia Castelani Lucas Eduardo Pilon Thamires Martins Claudia Rodrigues Pozzi Juliana Rodrigues Pozzi Arcaro 《Animal Science Journal》2015,86(3):340-344
Biofilm formation and antimicrobial resistance of Staphylococcus aureus are important virulence factors in cases of mastitis in dairy cows. However, few studies have investigated mastitis strains isolated from heifers. Within this context, the objective of the present study was to investigate biofilm formation on Congo red agar, the presence of the icaA and icaD genes by polymerase chain reaction (PCR), and the percentage of in vitro antimicrobial resistance of 110 S. aureus isolates from mammary gland secretions of heifers and cows with mastitis. PCR detected the icaA and icaD genes in 98% and 100% of isolates, respectively. However, only 55.5% of all isolates produced a biofilm on Congo red agar. Antimicrobial susceptibility testing revealed that 47.0% of isolates from heifers and 70.4% of isolates from cows were resistant to at least one of the antimicrobial agents tested. Resistance to penicillin and/or ampicillin was the most frequent (44.5%). These results indicate the need to implement prophylactic and control measures of mastitis for heifers. Heifers and cows can carry resistant strains with the capacity of biofilm production, a fact representing a threat to public health and animal well‐being and generating losses to dairy farmers. 相似文献
80.
Population pharmacokinetics of methadone hydrochloride after a single intramuscular administration in adult Japanese sika deer (Cervus nippon nippon)
下载免费PDF全文
![点击此处可从《Veterinary anaesthesia and analgesia》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Christopher Scala Amélie Marsot Marie‐Josée Limoges Yann Locatelli Nicolas Simon Jean‐Claude Alvarez 《Veterinary anaesthesia and analgesia》2015,42(2):165-172
ObjectiveTo assess the population pharmacokinetics of methadone in deer.Study designProspective non-randomized experimental trial.AnimalsTwelve healthy adult sika deer (nine males and three females).MethodsDeer received intramuscular administration of racemic methadone hydrochloride at 0.5 mg kg−1 or 1 mg kg−1. Plasma methadone and its metabolite 2-Ethylidene-1,5-Dimethyl-3,3-Diphenyl-Pyrolidine (EDDP) concentrations were determined by validated liquid chromatography coupled to tandem mass spectrometry methods, at times 0, 30 minutes, 1, 2, 3, 4, 5, 6, 8, 12 and 24 hours. Population pharmacokinetics analysis was undertaken using a non-linear mixed effects modelling (NONMEM).ResultsA two-compartment linear disposition model best described observed time-concentration profiles of methadone and EDDP. Population parameter estimates of methadone were elimination clearance (17.3 L hour−1), metabolic clearance (34.6 L hour−1), volume of distribution of compartment 1 (216.0 L) and volume of distribution of compartment 2 (384.0 L). Population parameter estimates of EDDP were elimination clearance (121.0 L hour−1), volume of distribution of compartment 3 (1.08 L) and volume of distribution of compartment 4 (499.5 L). The total clearance and total volume of distribution of methadone and EDDP were 51.9 L hour−1, 121.0 L hour −1, 600.0 L and 500.6 L, respectively. The methadone terminal elimination half-life was 8.19 hours. No adverse effects were observed after methadone administration.Conclusions and Clinical relevanceFollowing intramuscular injection, methadone was characterized by a large total volume of distribution, high systemic clearance and intermediate terminal half-life in sika deer. 相似文献