PPARγ mediates effects of diosgenin on proliferation and apoptosis in human glioblastoma U87MG cells     

ZHANG Tie-shan  SHANG Shu-yu  WANG Cong-ying 《园艺学报》2019,35(7):1176-1182

AIM:To investigate the effect of diosgenin (Dio) on the proliferation, apoptosis and expression of peroxisome proliferator-activated receptor γ (PPARγ) in human glioblastoma U87MG cells and its possible mechanism. METHODS:Human astrocytes (HA) and U87MG cells were cultured in vitro and treated with Dio (0, 10, 20, 30, 40 and 50 μmol/L) and GW9662 (5 μmol/L) for 48 h, and then the cell viability was detected by CCK-8 assay. Cell colony formation assay was used to assess the proliferation potential. Flow cytometry was used to analyze the cell cycle distribution and apoptosis. The mRNA expression level of PPARγ was measured by RT-PCR. Western blot was used to determine the protein levels of PPARγ, cyclin D1, cyclin E1, Bcl-2 and Bax. RESULTS:Dio had no significant influence on the viabi-lity of HA (P>0.05). However, Dio remarkably reduced the viability of U87MG cells in a dose-dependent manner (P<0.05) with IC₅₀ of 24.31 μmol/L. Meanwhile, Dio remarkably diminished colony formation ability (P<0.05), induced G₀/G₁ phase arrest of the cell cycle and apoptosis (P<0.05), up-regulated the expression of PPARγ at mRNA and protein levels, increased the protein level of Bax (P<0.05), and down-regulated the protein levels of cyclin D1, cyclin E1 and Bcl-2 (P<0.05) in a dose-dependent manner. However, these effects induced by Dio were inhibited by GW9662 (P<0.05), a specific inhibitor of PPARγ. CONCLUSION:Dio may inhibit proliferation and induce apoptosis in human glioblastoma U87MG cells most likely via up-regulating the expression of PPARγ, and then down-regulating the protein levels of cyclin D1, cyclin E1 and Bcl-2, and up-regulating the protein level of Bax.  相似文献   

冻融循环下红色砒砂岩水泥土力学性能试验研究     

耿凯强  李晓丽 《排灌机械工程学报》2021,39(6):643-648

为实现砒砂岩作为一种自然资源的潜在利用价值,提高砒砂岩有效利用,将鄂尔多斯市准格尔旗的红色砒砂岩加入不同掺量水泥制成砒砂岩水泥土,进行不同围压、不同冻融循环次数条件下的不固结不排水三轴试验,得到了砒砂岩水泥土的各力学参数的变化规律,并通过超景深三维显微镜对砒砂岩水泥土的细观结构进行观测.结果表明:砒砂岩水泥土的应力-应变曲线呈应变软化趋势,随水泥掺量的增加其黏聚力和内摩擦角呈线性增长的趋势.砒砂岩水泥土的内摩擦角受冻融循环影响较小,而黏聚力受冻融循环影响较大.水泥掺量越多,砒砂岩水泥土的抗冻性越好,但其脆性特征越明显.对细观结构的观测进一步验证了冻融使得低掺量水泥的固化土细观结构发生较大改变,导致内部空隙增大,而水泥土随水泥掺量的增加其内部结构更加致密,从而提高了水泥土的抗冻性.  相似文献   

紫云英与双季稻秸秆协同利用影响稻田土壤钾循环与平衡   总被引：2，自引：0，他引：2  

张帆  杨茜 《草业学报》2021,30(1):72-80

稻草还田和冬种绿肥是维持稻田地力和化肥替代的重要方式之一.本研究以在湖南酸性红黄泥和碱性紫潮泥两种典型稻田土壤上进行了2年的紫云英-双季稻定位试验为对象,分析紫云英和双季稻秸秆协同利用对稻田土壤K循环的影响.两土壤试验处理一致,包括稻田冬闲(FRR)、冬种紫云英(MvRR)及冬种紫云英与水稻秸秆协同利用(MvRR+St...  相似文献   

基于单螺杆膨胀机的发动机排气余热回收系统   总被引：4，自引：0，他引：4  

张红光  刘彬  陈研  杨凯  张健  王东芳 《农业机械学报》2012,43(5):27-31

针对发动机排气余热的特点,设计了有机朗肯循环(ORC)排气余热回收系统,采用单螺杆膨胀机作为动力输出装置,采用R245fa作为工质,提出了发动机排气余热利用率的概念和计算方法。结合发动机的试验数据,分析了ORC工质蒸发压力和发动机转速对ORC系统性能的影响,确定了适用于ORC系统的工质蒸发压力的最佳值。研究结果表明,当工质蒸发压力为3.0 MPa时,ORC系统能够在发动机全转速范围内正常工作,并且ORC系统的净输出功率最高可达12.1 kW,热力学第一定律效率最高可达11.27%,热力学第二定律效率最高可达25.8%,发动机排气余热利用率最高可达8.9%,发动机排气余热回收效果明显。  相似文献   

标定工况柴油机燃用小油桐油料的循环波动研究     

梁昱  王子玉  程秀围 《农机化研究》2012,34(7):219-223

在单缸水冷四冲程柴油机上针对不同的小油桐油料进行了标定工况试验,测录了多循环的瞬时气缸压力与高压油管燃油压力,对比分析了喷油与燃烧过程中各参数的循环波动。结果发现,小油桐油的喷油始点比柴油迟1°CA,喷油持续期相当,最高喷油压力要高出近5 MPa;滞燃期比柴油缩短约3°CA,燃烧始点早于柴油约2°CA,燃烧压力升高率明显比燃用柴油时低,最高燃烧压力也低于柴油。在循环波动上,柴油的滞燃期、燃烧始点均较稳定。燃用加热后的小油桐油平均指示压力的波动率较低,其燃烧过程,特别是后燃期较为稳定。  相似文献   

混合悬架半主动控制器设计与试验     

汪若尘  焦宇  钱金刚  丁仁凯  陈龙 《农业机械学报》2017,48(6):334-340

为改善传统被动悬架的动力学性能,回收悬架振动能量,设计了一种半主动混合悬架系统。建立1/4车动力学方程,分别研究馈能回路处于Boost模式和Buck模式时馈能回路内电流的变化情况,并分析MOS管占空比对直线电动机电磁阻尼力的影响。在此基础上,引入基于天棚和地棚混合控制的半主动控制策略。提出半主动控制参考力的概念,并运用粒子群算法确定其最优控制参数。通过对不同工作模式下电路电流的追踪,达到对电动机电磁阻尼力实时控制的目的。接着运用Simulink仿真搭建混合悬架系统模型,分别进行动力学性能、馈能性能以及电流跟踪控制效果对比。仿真结果表明,半主动混合悬架能够在改善车辆动力学性能的同时回收部分振动能量,所设计的半主动控制器对电流有较好的控制效果。最后,进行台架试验,通过对比试验结果验证了仿真结果的正确性。  相似文献   

Organic carbon content under different types of land use and soil in peninsular Spain   总被引：16，自引：0，他引：16  

Juan Carlos Rodríguez-Murillo 《Biology and Fertility of Soils》2001,33(1):53-61

  相似文献   

Amino acids in grassland soils: Climatic effects on concentrations and chirality   总被引：6，自引：0，他引：6  

W. Amelung  X. Zhang  K.W. Flach   《Geoderma》2006,130(3-4):207-217

The response of soil organic nitrogen (SON) dynamics to climate may partly be deduced from changes in the concentration and origin of the major N constituents in soil, such as amino acids. In this study, we determined the enantiomers of bound amino acids in 18 native grassland soils (0–10 cm) that were sampled along a transect from central Saskatchewan, Canada, to Southern Texas, USA. Mean annual temperature (MAT) ranged from 0.9 to 23.4 °C and mean annual precipitation (MAP) from 300 to 1308 mm. d-alanine and d-glutamic acid served as markers for the bacterial origin of SON. The d-content of lysine, phenylalanine, and aspartic acid indicated an ageing of the respective SON forms. Deuterium labeling was applied to account for hydrolysis-induced racemization reactions. We found that the concentration of the bacterial biomarkers was weakly but significantly parabolically related to MAT, as previously reported for microbial-derived amino sugars. The age markers d-lysine, d-phenylalanine, and d-aspartic acid comprised 2–15% of the respective l-form. The presence of these compounds demonstrated that the structures that contained these d-enantiomers had survived microbial attack, i.e., these hydrolyzable SON forms were conserved in soil despite a living environment. First estimates indicate that the mean residence time of the lysine-containing organic matter forms extend beyond a century. Within this time-scale we did not find that climate significantly affects the degree of ageing of SON constituents in the studied topsoils.  相似文献   

磷在富营养化湖泊沉积物-水界面的循环   总被引：22，自引：4，他引：22  

高丽  周健民 《土壤通报》2004,35(4):512-515

沉积物中部分固定的磷可通过分解或溶解作用而释放磷酸盐到沉积物间隙水中;再生的磷可能释放到上覆水体中,或在沉积物中作为一种自生相而再沉淀,或被沉积物的其它组分所吸附。本文描述了磷在沉积物-水界面的迁移过程,并对影响磷循环的因子及其磷自沉积物释放的机制作一简要概述。  相似文献   

饲粮锌、硒和维生素E水平对后备母猪生长发育及生殖激素的影响     

梁明振  梁贤威  卢克焕  杨炳壮  文崇利  黄海岭  梁坤  林宝东 《广西农业生物科学》2004,23(3):222-226

选择日龄、体况较一致的二元杂(长白×约克)后备母猪27头,采用L9(34)正交表安排试验,共9个试验组,每组3个重复。饲粮锌、硒和维生素E均设置3个水平,分别为:锌(45.0mg/kg,85.0mg/kg,125mg/kg);硒(0.10mg/kg,0.25mg/kg,0.40mg/kg);维生素E(15IU/kg,30IU/kg,45IU/kg),目的是研究饲粮微量元素锌、硒和维生素E对后备母猪生长发育和血清生殖激素参数的影响规律。结果表明在喂给含锌量85mg/kg,含硒量0.25mg/kg,含维生素E量45IU/kg饲粮时:(1)后备母猪的初次发情时间最早,分别为(187.4±6.6)d,(185.9±8.4)d,(189.1±7.3)d(P<0.05);(2)血清雌激素及孕激素浓度在不同日龄情况下均显示最高水平(P<0.05或P<0.10)。综合所考察的参数,后备母猪饲粮中含锌85mg/kg,含硒0.25mg/kg,含维生素E45IU/kg是适当的组合用量。  相似文献