切花月季无土容器芽接快繁的定量研究     

汪有良  赵永艳 《江苏林业科技》1998,25(1):17-21

研究了切花月季无土容器芽接快繁种苗的新方法。研究表明,采用本方法生产切花月季种苗,不仅可以大幅度提高芽接成活率和成苗质量,杜绝根癌病危害,而且可以减轻劳动强度,提高生产效率,延长种苗生产和种苗销售的时间。无土容器芽接快繁的优化定量程序是：１．以直径０．５～０．７ｃｍ的粉团蔷薇新梢节段于盆内集中扦插以繁殖砧木;２．生根砧木双株分盆定植于无土基质中,并使其单个分枝朝向为南向;３．选５小叶腋芽并用大开门芽接法将其接在砧木分枝正下方;４．悉数保留砧木枝叶,并作扭曲折伏处理,摘除其顶芽和腋芽;５．接芽长至２～３ｃｍ时施用高纯度速效全成分营养液。  相似文献   

Evidence of Migration and Endophytic Presence of Agrobacterium tumefaciens in Rose Plants   总被引：2，自引：0，他引：2  

Rubén Martí  Jaime Cubero  Antonio Daza  Jaime Piquer  Carmen I. Salcedo  Clara Morente  María M. López 《European journal of plant pathology / European Foundation for Plant Pathology》1999,105(1):39-50

Agrobacterium tumefaciens was isolated from stem tumors of several rose cultivars showing that the bacterium is the causal agent of aerial galls in rose plants. No differences were observed in the characteristics of the Agrobacterium isolates from crown or aerial galls. Stem inoculation of ten rose cultivars showed that all of them were susceptible to A. tumefaciens but differences in the size of the resulting tumors were observed. The movement of A. tumefaciens in rose plants was demonstrated using two wild type strains and two antibiotic resistant mutants. Three months after inoculation, the inoculated strains were recovered in the roots, crown and below and above the inoculation site but low numbers of pathogenic Agrobacterium cells were isolated. New tumors appeared in 5% of the noninoculated wounds. A. tumefaciens was isolated from the stem at different distances from the tumor in naturally infected plants. In symptomless commercial plants, the isolation from the roots, crown and at different stem levels demonstrated the existence of systemic and latent infections in rose. Direct isolation using a nonselective and selective media with or without a previous enrichment step were efficient methods for isolating tumorigenic Agrobacterium from the different parts of rose plants.  相似文献   

Evaluation of different types of compost tea to control rose powdery mildew (Sphaerotheca pannosa var. rosae)     

Samin Seddigh  Leila Kiani 《国际虫害防治杂志》2018,64(2):178-184

Powdery mildew (Sphaerotheca pannosa var. rosae) is an important foliar disease which is found in all rose-producing greenhouses. An experiment was carried out in a commercial greenhouse in Hashtgerd, Iran, in 2014 to investigate whether compost teas can be used to control this disease. Taguchi's design of experiment (Taguchi's DOE) method was used to plan a minimum number of experiments and optimization to control rose powdery mildew. The test included three factors, namely the different types of compost tea (aerated and non-aerated compost tea, aerated and non-aerated vermicompost tea), the dosage (1:8 and 1:16, compost:water), and the number of spray applications (once and twice per week). The experiment was set up as an orthogonal array L₈ with three replicates. Based on the experimental results, the optimum treatment combination for controlling rose powdery mildew was found to be aerated compost tea with 1:8 dilution with an application of twice per week.  相似文献   

有机溶剂萃取与SPME提取的玫瑰水挥发性成分对比分析     

宋婉瑶  刘玉亮  姚雷 《上海交通大学学报(农业科学版)》2016,(4):57-64

玫瑰水(rose water)是玫瑰精油生产过程中的一种副产品,因其成分天然、香气怡人,以及补充皮肤水分和缓解皮肤衰老的功效,而被广泛地应用于食品、化妆品和护肤品中。为了探索玫瑰水挥发性成分分析的科学方法,采用有机溶剂萃取和固相微萃取(SPME)2种方法萃取2种国产玫瑰水中的挥发性成分。气相色谱质谱仪(GC/MS)分析表明,固相微萃取法萃取得到的2种玫瑰水中的香气成分种类(分别为31种和28种)明显多于有机溶剂萃取法萃取出的香气成分种类(分别为23种和21种),前者可以更全面地反应玫瑰水的香气成分构成,能有效地萃取玫瑰水香气成分中沸点较低、易挥发或是化学性质活泼的萜烯类、醚类和硫醚类化合物;且与有机溶剂萃取法相比,操作简单,省时。  相似文献   

The potential of pet‐grade poultry by‐product meal to replace fish meal in the diet of the juvenile spotted rose snapper Lutjanus guttatus (Steindachner, 1869)     

C. Hernández  Y. Sanchez‐Gutierrez  R. W. Hardy  A. Benitez‐Hernández  P. Domínguez‐Jimenez  B. González‐Rodríguez  L. Osuna‐Osuna  O. Tortoledo 《Aquaculture Nutrition》2014,20(6):623-631

A 12‐week feeding trial was conducted to examine the replacement of fish meal with pet‐grade poultry by‐product meal (PBM‐PG) in the spotted rose snapper Lutjanus guttatus diet. Five experimental diets were formulated to contain graded levels of PBM‐PG at proportion of 250, 500, 75 or 900 g kg^?1. The control diet contained sardine fish meal as the main protein source. Four groups of 15 randomly assigned L. guttatus juveniles were fed to satiation 3 times day^?1. Except for the fish fed the PBM‐PG90 diet, the growth performance, survival and feed utilization efficiency of the experimental fish were not significantly lower than those of the control fish. The dietary level of PBM‐PG did significantly affect the haematological characteristics (P < 0.05). The dietary dry matter and protein apparent digestibility coefficients (ADCs) decreased with increasing dietary PBM‐PG. High values for lipid ADCs were observed in all diets, with significant differences among the dietary treatments. The fish whole‐body protein, moisture, lipid and ash contents were not affected by the inclusion of dietary PBM. These results indicate that high‐quality terrestrial PBM can successfully replace more than half of the marine fish meal protein in the L. guttatus diet.  相似文献   

茶香月季新品种‘美人香’     

王勋曜  俞红强游 捷 刘青林 李 虹吴春莹  马宏宇谷士国 《园艺学报》2014,41(4):809-810

 茶香月季新品种‘美人香’是由‘香欢喜’ב艾丽’杂交育成。花朵淡粉色,重瓣,香气浓郁,平均花径9.9 cm,连续开花性状好。生长势强,不太耐高温高湿,抗病性较强,适宜栽培于以北京气候特点为主的华北地区。  相似文献   

野生玫瑰抗旱性地理变异的初步研究   总被引：2，自引：0，他引：2  

朱红梅  丰震  杨科家  莫镇华  尹海燕 《中国农学通报》2009,25(12):172-176

以吉林珲春,山东牟平,山东荣成3个地理种源的野生玫瑰为材料进行盆栽试验,研究了干旱胁迫下玫瑰的生理生化变化与抗旱性强弱。结果表明,干旱胁迫条件下, 3个种源野生玫瑰的5个抗旱生理生化指标的变化趋势基本一致,但各指标的变化程度因种源不同而异。通过“坐标综合评定方法”对5个生理生化指标进行综合评定,得到3个种源野生玫瑰的抗旱性强弱等级依次为吉林珲春,山东牟平,山东荣成。  相似文献   

16个中国传统月季品种的核型分析   总被引：7，自引：2，他引：5  

罗乐  张启翔  白锦荣  潘会堂  李卉  王琼 《北京林业大学学报》2009,31(5):90-95

采用常规压片法,对16个特有的中国传统月季品种进行核型分析。结果表明:①16个品种的倍性分为3类,‘月月红’、‘月月粉’、‘绿萼’、‘匍匐红’4个品种为二倍体,2n=2x=14;‘春水绿波’、‘金瓯泛绿’、‘桔囊’、‘青莲学士’、‘四面镜’、‘一季粉’、‘一品朱衣’、‘羽仕妆’8个品种为三倍体,2n=3x=21;‘大富贵’、‘玉玲珑’、‘紫香绒’3个品种为四倍体,2n=4x=28;‘牡丹月季’品种为混倍体,包含三倍体、四倍体两种稳定核型。品种的倍性演化与研究过的野生蔷薇、现代月季品种的演化基本一致,倍性与表型呈一定的正相关性。②核型不对称系数为56.70%～63.70%。③核型类型包括1A、2A、1B、2B 4种类型,所有品种核型都由其中部或近中部的着丝点染色体组成。   相似文献   

月季ITS反应体系的优化     

张慧  唐开学  邱显钦  蹇洪英  王其刚  张颢 《华中农业大学学报》2009,28(6)

以古老月季品种‘玉玲珑'为试材,利用CTAB法对月季进行总DNA提取,对其PCR扩增条件中的主要因素进行了多梯度的优化,总反应体系为25μL,其中引物浓度为0.4μmol/L、Mg~(2+)浓度为2.0 mmol/L、dNTP为0.2 mmol/L、Taq酶用量为1.0 U、DNA模板用量为70 ng.利用该优化体系对部分月季品种和野生种进行PCR扩增和电泳检测,扩增条带清晰,结果稳定,测序结果理想,表明该体系适用于月季的ITS序列分析.  相似文献   

浅析玫瑰隐喻的嬗变     

蒲筱梅  孔桂英 《云南农业大学学报(自然科学版)》2009,3(4):99-102

玫瑰是西方文化中最著名的文化符号之一,它所承载的隐喻意义在汉民族文化中并不为人熟悉。从符号学理论的能指、所指、符号三者之间的关系出发,考察玫瑰如何从作为园艺实体的花卉（能指）,成为充满丰富文化内涵的隐喻（所指）,并相互关联,成为一个符号。同时,解读玫瑰的神话文本渊源与其后相关的重要文本之间的“文本相互作用性”,探讨玫瑰隐喻作为文化符号的嬗变。  相似文献