皱皮香猪HAS2基因SNPs的鉴定及生物信息学分析   总被引：1，自引：1，他引：0  

吴行雕  石道宏  黄世会  牛熙  李升  王嘉福  冉雪琴 《畜牧兽医学报》2021,52(12):3390-3402

为了探究皱皮香猪全身性皮肤褶皱是否与透明质酸合成酶2（hyaluronan synthase 2,HAS2）基因变异有关,本研究以普通香猪为对照,采用特异性PCR方法克隆HAS2基因的外显子编码区,应用生物信息学方法分析测定基因编码区的碱基变异,检测变异位点在群体中的分布频率。结果显示,皱皮香猪HAS2基因中检测到4个SNPs位点：位于外显子1的T221A错义突变（导致亮氨酸替换为赖氨酸）和A228G同义突变,位于外显子2的T183C和外显子4的C537T同义突变。生物信息学分析发现,T221A和A228G位点构成4种单倍型,其中T-A为皱皮香猪群体的主要单倍型（27.5%）,且在皱皮香猪群体中紧密连锁（r²=0.253）,而且T221A和A228G突变可引起mRNA自由能和蛋白质结构发生变化,TA组合的自由能为-573.29 kJ·mol^-1,TG组合的自由能为-580.89 kJ·mol^-1,AG组合的自由能为-572.66 kJ·mol^-1;AA组合的自由能为-571.03 kJ·mol^-1;蛋白序列中亮氨酸替换为赖氨酸后,α螺旋由35.52%降为32.97%,自由卷曲由43.17%升至44.51%,同时引起蛋白序列三级结构的改变。位点T221A和A228G在香猪群体中呈现出丰富的多态性,均表现出3种基因型;关联分析结果显示,皱皮香猪T221A位点的A等位基因频率显著高于普通香猪（P<0.05）,A228G位点中皱皮香猪的G等位基因频率极显著高于普通香猪（P<0.01）。T183C位点的C等位基因频率和C537T位点的T等位基因频率在皱皮香猪和普通香猪间未达到显著性差异（P>0.05）。研究结果揭示,HAS2基因的外显子1中发生的两个碱基变异可能影响基因转录后mRNA的稳定性以及蛋白的三维结构,影响HA的合成量,并与皱皮香猪体侧皮肤不均匀增厚且发生褶皱有关。  相似文献   

Ecological Technology Strategy-the Typologic Analysis and Research of Double-Skin Facade     

WANG Xue-song 《保鲜与加工》2003,(6):5-9

As a renovation envelope, double-skin facade has the characteristics of environmental function and unique aesthetic effect. In this paper, the composition and functional characteristics of double skin facade is introduced briefly, its development history and a detailed typologic analysis combined with typical cases is reviewed. It is pointed out that the double skin facade has a bright future in 21~(st) century and the function and aesthetics are the footstone for its typologic research.  相似文献   

鹿茸发育的组织来源及其相互作用机制研究进展     

鲁晓萍 《特产研究》2014,(1):61-63

本综述介绍了鹿茸发育的组织来源及其相互作用机制的研究进展,阐明了鹿茸发育的组织细胞特性,进一步分析了鹿茸发育机制中待解决的问题。  相似文献   

Dermatophilus congolensis Infection in Goats in Tanzania     

Msami HM  Khaschabi D  Schöpf K  Kapaga AM  Shibahara T 《Tropical animal health and production》2001,33(5):367-377

When goats in Eastern Tanzania were screened for skin diseases, Dermatophilus congolensis was isolated from the skin lesions in 8 of 484 animals examined. In one severely affected case, the disease was also characterized by histological studies (Gram stain, Giemsa stain and routine HE studies) and electron microscopy. The histological picture was characterized by hyperkeratosis, parakeratosis, acanthosis, folliculitis and an inflammatory cellular reaction involving the epidermis. Gram stain and Giemsa stain revealed longitudinal and transverse branching filaments in the deeper layers of the epidermis. Ultrastructural studies demonstrated D. congolensis in various morphological forms, ranging from filamentous to tuber-shaped structures, mixed with numerous coccoid bodies of variable size. In some instances, the organisms were geometrically arranged in parallel rows of beading and were present in and among the degenerated epithelial cells. Several host cells showed degenerative changes. Ticks present on the goats were Amblyomma variegatum, Rhipicephalus evertsi, Rhipicephalus pravus and Boophilus sp. The clinical signs, pathological lesions, diagnosis, epidemiology and pathogenesis of the disease are discussed.  相似文献   

Spongiotic vesicular dermatitis as a cutaneous reaction pattern in seven horses     

Hargis AM  Clark EG  Duclos DD  Leclerc S  West K 《Veterinary dermatology》2001,12(5):291-296

Over a 6-year period seven adult horses of different breeds and genders developed multifocal, exudative, oozing dermatitis characterized histologically by epidermal spongiotic vesicles and perivascular eosinophilic, neutrophilic and mixed mononuclear inflammation. Three horses were pruritic. Systemic disease was not noted. Two horses had a history of recurrent urticaria (hives) and one horse had nodules or welt-type lesions that progressed to exudative, oozing lesions. Interepithelial immunoglobulin (Ig)G was detected by avidin-biotin complex-peroxidase staining, but the pattern of staining was more consistent with epithelial oedema than specific IgG deposition associated with pemphigus. The exudative oozing lesions developed under circumstances suggesting that dermal oedema progressed to intracellular and intercellular epidermal oedema, which in turn progressed to the spongiotic vesicular epidermal lesions.  相似文献   

The histological lesions of Trichophyton mentagrophytes var erinacei infection in dogs     

Fairley RA 《Veterinary dermatology》2001,12(2):119-122

A retrospective study of the histological features of four cases of canine Trichophyton menta-grophytes var erinacei infection is reported. In all four dogs the initial lesions affected the dorsal muzzle and in two dogs the lesions spread to more distant sites on the body. Clinically, the lesions were characterized by scaling, crusting and hair loss. Histologically, the main lesions were characterized by acanthosis, epidermal, ostial and infundibular hyperkeratosis, serocellular crusting, mural folliculitis and furunculosis. Fungal hyphae were usually sparse and often difficult to see in haematoxylin and eosin stained sections. When visible they were seen in the epidermal, ostial and infundibular scale and, less frequently, within hair shafts.  相似文献   

Sulphido-leukotriene production from peripheral leukocytes and skin in clinically normal dogs and house dust mite positive atopic dogs   总被引：1，自引：0，他引：1  

Marsella R  Nicklin CF 《Veterinary dermatology》2001,12(1):3-12

Pathogenesis of canine atopy has not been completely elucidated. In humans, sulphido-leukotrienes (s-LT) play a role in atopy, and increased production of s-LT occurs in the skin and peripheral leukocytes after allergen challenge. The study population included 16 clinically normal and 13 atopic dogs. All atopic dogs had in common a positive reaction (4+) to the intradermal injection of house dust mite (allergen of reference). Blood samples and skin biopsies were collected. Sulphido-LT synthesis by peripheral leukocytes after stimulation was measured, and no statistically significant difference was found between clinically normal and atopic dogs. Sulphido-LT concentrations in skin samples from stimulated and unstimulated sites were measured, and no statistically significant difference was detected between clinically normal and atopic dogs or between lesional and nonlesional skin within the atopic group. Clinical signs of atopic dogs were graded by owners and no correlation was found between their severity and cutaneous concentrations of s-LT. In this study there was no increase in s-LT synthesis in atopic dogs.  相似文献   

Novel Canine Tumour Suppressor Gene p53 Mutations in Cases of Skin and Mammary Neoplasms     

Mayr  B.  Reifinger  M.  Alton  K. 《Veterinary research communications》1999,23(5):285-291

Exons 4 to 8 of the tumour suppressor gene p53 were analysed in 25 skin and 25 mammary tumours of 50 dogs. A 1 bp deletion (ACCAC) was detected in codon 89 in exon 4 in a squamous cell carcinoma. A missense mutation CGCCAC (argininehistidine) was present in codon 162 in exon 5 in a mammary adenocarcinoma. Moreover, a silent mutation occurred in codon 103 (serine) of exon 4 in a mammary adenoma. The somatic nature of the three mutations was demonstrated.  相似文献   

The influence of the hemoglobin concentration in growing mink kits on certain skin characteristics     

Anne Nveri  Jouni Kangas  Jaakko Mkel 《Acta veterinaria Scandinavica》1984,25(1):50-56

The relationship between some skin characteristics and hemoglobin values during the growth period were statistically examined in standard male minks. Significant correlations) (P < 0.05–0.001) were found between the hemoglobin concentrations determined at 3 points during the growth period (in July, August and November) and the density and colour of underfur, and skin length, respectively. According to the study even the early hemoglobin values in July and August have a significant influence on the size and quality of the pelt. Mink kits with low hemoglobin values are liable to develop a winter fur coat with defective pigmentation and hair structure of the underfur. Anemic minks develop the most extreme stage of the pigmentation defect and these so-called cotton fur skins have little value in the fur garment industry. When these abnormal hemoglobin values are recognized at an early stage, cotton fur skins may be avoided, and larger skins of better quality may be produced.  相似文献   

Cyclosporin: applications in small animal dermatology     

Robson DC  Burton GG 《Veterinary dermatology》2003,14(1):1-9

Abstract Cyclosporin has been increasingly used for the treatment of skin diseases in small animals. Reported uses include the treatment of atopy, cutaneous lupus erythematosus, feline acquired alopecia resembling pseudopelade of humans, pemphigus erythematosus, pemphigus foliaceus, perianal fistulae and sebaceous adenitis. In addition, cyclosporin has been used anecdotally for several other skin diseases. Few side effects have been noted at doses therapeutic for dermatologic diseases. Current suggestions for monitoring, and the value of trough cyclosporin serum concentrations for prediction of toxicity and efficacy are discussed.  相似文献