家庭猪场防控非洲猪瘟的关键点分析     

刘涛 《猪业科学》2021,38(10):38-41

非洲猪瘟对家庭猪场的生产稳定性带来极大的挑战。文章分析了家庭猪场防控非洲猪瘟存在的问题及对关键点针对性的防控措施。  相似文献   

切断传染源与传染途径对防治猪病重要性     

由皓月  姜娓娓 《畜牧兽医科学(电子版)》2021,(4):154-155

近年,生猪养殖产业不断向着集约化规模化方向发展,养殖密度增加的同时,各种传染性疾病呈现高发趋势。猪传染性疾病发生后,会造成生长发育不良,行动迟缓,生理指标失衡,甚至引发死亡,带来的经济损失不可估量,某些人畜共患病还会威胁人类的身体健康。在疫病防控过程中,需要从传染源、传播途径和易感群体等角度出发,减少养殖环境病原数量,切断病原传播途径,保护易感群体,确保生猪健康生长。该文主要结合一个乡镇的疫病流行特点,分析切断传染源和传播途径对防治猪病发生流行的重要性。  相似文献   

规模化猪场非洲猪瘟防控     

范良志 《畜牧兽医科学(电子版)》2021,(5):159-160

非洲猪瘟疫情已经成为制约养猪业发展的头号杀手,生猪稳产保供任务引起高度重视,因此,防控非洲猪瘟疫情是各级政府和养猪企业的重点工作之一,该文主要论述规模化猪场非洲猪瘟防控措施。  相似文献   

非洲猪瘟疫情防控难点与对策     

喻明辉 《畜牧兽医科学(电子版)》2021,(5):169-170

目前肉制品的健康情况备受人们关注,而猪肉是人们餐桌上必不可少的肉制品之一,猪只的安全饲养对人类的生命健康有重要的影响。在饲养猪只中,可发生多种传染病,其中最主要的一种是非洲猪瘟,该传染病不仅具有高热性的特点,还具有高度接触性的特点,再加上目前对于该种疾病还没有特效的疫苗,以及有效的治疗措施,因此,做好该种疾病的预防工作至关重要。该文主要论述非洲猪瘟进行论述,并制定相应的防控措施。为减少猪只发病几率奠定良好的基础。  相似文献   

猪粪好氧堆肥条件的研究   总被引：2，自引：2，他引：2  

吴银宝  廖新  刘胜安  汪植三  梁敏  吴启堂  黄焕忠  周立祥 《农业工程学报》2001,17(7):153-157

本试验研究了在不同的调理剂、通气方式、碳氮比和添加剂等条件下猪粪堆肥的腐熟进程。结果表明：稻草比稻壳更有利于猪粪的堆肥腐熟；机械通风同人工翻堆相比，各项指标无显著差异(P＜0.05)，而堆制结束后物理性状要比人工翻堆试验组差；高C/N有利于堆肥的升温及腐殖化过程，对堆肥进程无影响，但稻草加入量大会增加堆肥的成本；除臭剂及菌剂的加入对堆肥的大多数化学性状影响不大，但可明显改善堆肥腐熟后的物理性状并减少臭味。除稻壳试验组外，其它各组在第五周即可达到腐熟的要求。  相似文献   

Establishment and Application of Nested RT-PCR Assay for Detection of Classical Swine Fever Virus     

LIU Mei-fen  WANG Shu-juan  YAN Ruo-qian  WANG Dong-fang  CAO Wei-wei  ZHAO Xue-li  LI Qin-nan  LI Ning 《中国畜牧兽医》2016,43(9):2285-2290

  相似文献   

猪瘟病毒石门株在ST细胞连续传代后E0基因变异分析     

孙永科  胡超英  林明星  陆欣然  杨玉艾 《动物医学进展》2016,(6):13-17

为了解猪瘟病毒(CSFV)石门(Shimen)株连续传代后变异情况,根据已发表的CSFV E0基因序列(AF092448.2),设计合成引物,以在ST细胞中连续传代培养的CSFV石门株细胞毒总RNA为模板,每5代通过RT-PCR方法扩增病毒的E0基因,测定其核苷酸序列和氨基酸序列,用DNA Star软件分析比较原代、5代、10代、15代、20代、25代接毒细胞中CSFV E0基因的变异性。结果显示,接种病毒第10代的病毒E0基因在630核苷酸位点出现变异,第15代病毒在632核苷酸位点出现变异。本研究通过分子生物学方法发现CSFV Shimen株在ST细胞连续传代过程中E0基因能保持遗传的稳定性。  相似文献   

Demonstrating freedom from disease using multiple complex data sources 2: case study--classical swine fever in Denmark     

Martin PA  Cameron AR  Barfod K  Sergeant ES  Greiner M 《Preventive veterinary medicine》2007,79(2-4):98-115

A method for quantitative evaluation of surveillance for disease freedom has been presented in the accompanying paper (Martin et al., 2007). This paper presents an application of the methods, using as an example surveillance for classical swine fever (CSF) in Denmark in 2005. A scenario tree model is presented for the abattoir-based serology component of the Danish CSF surveillance system, in which blood samples are collected in an ad hoc abattoir sampling process, from adult pigs originating in breeding herds in Denmark. The model incorporates effects of targeting (differential risk of seropositivity) associated with age and location (county), and disease clustering within herds. A surveillance time period of one month was used in the analysis. Records for the year 2005 were analysed, representing 25,332 samples from 3528 herds; all were negative for CSF-specific antibodies. Design prevalences of 0.1-1% of herds and 5% of animals within an infected herd were used. The estimated mean surveillance system component (SSC) sensitivities (probability that the SSC would give a positive outcome given the animals processed and that the country is infected at the design prevalences) per month were 0.18, 0.63 and 0.86, for among-herd design prevalences of 0.001, 0.005 and 0.01. The probabilities that the population was free from CSF at each of these design prevalences, after a year of accumulated negative surveillance data, were 0.91, 1.00 and 1.00. Targeting adults and herds from South Jutland was estimated to give approximately 1.9, 1.6 and 1.4 times the surveillance sensitivity of a proportionally representative sampling program for these three among-herd design prevalences.  相似文献   

Hepatitis E virus in Cuba: A cross-sectional serological and virological study in pigs and people occupationally exposed to pigs     

Maria Caridad Montalvo Villalba  Chantal J. Snoeck  Licel de los Angeles Rodriguez Lay  Aurélie Sausy  Dayesi Hernández López  Marite Bello Corredor  Barbara Marrero Sanchéz  Judith M. Hübschen 《Zoonoses and public health》2023,70(1):58-68

Surveillance of hepatitis E virus (HEV) in risk groups is an important strategy to monitor its circulation pattern and to timely detect changes thereof. The aims of this cross-sectional study were to estimate the prevalence of HEV infections in pigs and humans from different regions of the country, to identify risk factors for increasing anti-HEV IgG prevalence and to characterize HEV strains. The presence of anti-HEV antibodies was assessed by commercial ELISA in serum samples from the general population, farm and slaughterhouse employees, as well as pigs sampled in the three regions of Cuba from February to September 2016. Overall, individuals with occupational exposure to swine or swine products (70/248, 28.2%) were 4 times more likely to be seropositive compared to the general population (25/285, 8.7%; OR: 4.18; p < .001). Within the risk group, risk factors included age, number of years working in a professional activity with direct exposure to swine, geographic region and distance between residence and closest professional swine setting, while wearing gloves had a protective effect. Prevalence of total anti-HEV antibodies in swine was 88.2% (165/187) and HEV RNA was detected by real-time RT-PCR in 9.2% (16/173) swine stools. All HEV strains sequenced clustered within genotype 3. Some strains clearly belonged to subtype 3a, while another group of strains was related with subtypes 3b and 3 k but partial HEV sequences did not allow unequivocal subtype assignment. These findings suggest that the high HEV exposure in Cuban individuals with swine-related occupations could be due to enzootic HEV in certain regions, direct contact with infectious animals or their products as well as environmental contamination.  相似文献   

条件性敲除D1133L基因的重组非洲猪瘟病毒的构建及增殖特性     

张婷  冯涛  杨金柯  郝雨  杨行  张大俊  史喜绢  闫文倩  陈玲玲  刘湘涛  郑海学  张克山 《畜牧兽医学报》2023,54(2):706-714

前期研究初步表明非洲猪瘟病毒(ASFV)编码的D1133L基因对ASFV复制至关重要,本研究拟进一步探究D1133L在ASFV复制中的作用。利用同源重组技术结合大肠杆菌lac阻遏操作系统实现条件性敲除D1133L基因,以pUC118为骨架重组转移载体ASFVΔi130,将重组转移载体转染骨髓源巨噬细胞(BMDMs),以ASFV CN/GS/2018为亲本毒株感染BMDM,在β-D-硫代半乳糖苷(IPTG)存在的条件下,经绿色荧光和PCR鉴定,获得条件性敲除D1133L重组毒株vD1133Li。利用荧光显微镜观察该重组毒株与亲本毒株在猪肺泡巨噬细胞(PAMs)中的复制差异,利用qPCR技术比较重组病毒与亲本毒株的复制差异,分析vD1133Li在无IPTG情况下回补D1133L蛋白后与在IPTG诱导情况下的复制差异。结果显示：本研究成功构建了条件性敲除D1133L的ASFV重组病毒vD1133Li,重组毒株不表达D1133L,在IPTG诱导下复制能力显著低于亲本毒株;在稳定表达D1133L的MA-104细胞系中,vD1133Li复制能力恢复。综上所述,D1133L基因对于ASFV复制至关重...  相似文献