安哥拉山羊Hoxc9基因克隆与序列分析     

赖双英  张燕军  李金泉  尹俊  菊林花 《江西农业大学学报》2007,29(6):884-886

根据已报道的Hoxc9基因序列保守区设计3对引物,利用PCR技术从安哥拉山羊血液基因组DNA中扩增Hoxc9基因序列。目的片段纯化后连接到PMD19-T载体上,经鉴定得到重组质粒。测序结果通过与Gen-Bank数据库中序列比对分析,确定该序列为Hoxc9基因,核苷酸序列长3224 bp,包括1个内含子和2个外显子,cDNA序列长783 bp,编码260个氨基酸。与哺乳动物氨基酸序列的同源性非常高,达到99.2%以上,同斑马鱼和日本鳉的同源性较低。  相似文献   

团头鲂(Megahbrama amblycephala) Caspase 9 基因全长cDNA的克隆及在氨氮胁迫下的表达分析          下载免费PDF全文

章琼  孙盛明  李冰  蒋高中  朱健  戈贤平 《渔业科学进展》2016,37(1):36-45

为了解团头鲂(Megahbrama amblycephala) Caspase 9基因序列特征及其在氨氮胁迫过程中的作用,应用末端快速扩增(Rapid amplification of cDNA ends,RACE)技术克隆得到团头鲂Caspase 9基因全长1613 bp的cDNA序列,包括185 bp的5 '末端非翻译区(Untranslated regions,UTR)、96 bp的3'UTR、1332 bp的开放阅读框(Open Reading Frame,ORF).氨基酸多序列比对显示,平均同源性为77.74％,表明团头鲂Caspase9基因具有较高的保守性;系统进化分析显示,该基因氨基酸序列与其他鱼类Caspase 9聚为一支,并与锦鲤(Cyprinus carpio) Caspase 9亲缘关系较近;荧光定量PCR结果显示,Caspase 9基因在团头鲂各组织中均有表达,在脑中表达量最高,在肌肉中表达量最低,同时,在胁迫和恢复过程中,该基因在肝和脑中的表达规律相似,均在胁迫期间出现表达量上调,整体呈类似波浪形表达谱.研究结果表明,氨氮胁迫下,Caspase 9基因参与团头鲂的免疫防御,并与细胞凋亡分子过程有关.本研究结果可为进一步了解团头鲂应答氨氮胁迫分子机制提供理论依据.  相似文献   

Targeted Knock-in of a Fluorescent Protein Gene into the Chicken Vasa Homolog Locus of Chicken Primordial Germ Cells using CRIS-PITCh Method     

Ryo Ezaki  Kennosuke Ichikawa  Mei Matsuzaki  Hiroyuki Horiuchi 《The Journal of Poultry Science》2022,59(2):182

In chickens, primordial germ cells (PGCs) are effective targets for advanced genome editing, including gene knock-in. Although a long-term culture system has been established for chicken PGCs, it is necessary to select a gene-editing tool that is efficient and precise for editing the PGC genome while maintaining its ability to contribute to the reproductive system. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) and CRISPR-mediated precise integration into the target chromosome (CRIS-PITCh) methods are superior as the donor vector is easier to construct, has high genome editing efficiency, and does not select target cells, compared to the homologous recombination method, which has been conventionally used to generate knock-in chickens. In this study, we engineered knock-in chicken PGCs by integrating a fluorescent protein gene cassette as a fusion protein into the chicken vasa homolog (CVH) locus of chicken PGCs using the CRIS-PITCh method. The knock-in PGCs expressed the fluorescent protein in vitro and in vivo, facilitating the tracking of PGCs. Furthermore, we characterized the efficiency of engineering double knock-in cell lines. Knock-in cell clones were obtained by limiting dilution, and the efficiency of engineering double knock-in cell lines was confirmed by genotyping. We found that 82% of the analyzed clones were successfully knocked-in into both alleles. We suggest that the production of model chicken from the knock-in PGCs can contribute to various studies, such as the elucidation of the fate of germ cells and sex determination in chicken.  相似文献   

加工萝卜蜀萝9号(038A/C9274)的选育与应用   总被引：1，自引：0，他引：1  

冉茂林  陈天清  雍晓平等 《江西农业学报》2014,(1):44-46,52

介绍了适合四川蔬菜加工的萝卜品种蜀萝9号的亲本选育经过、组合配制、品比试验、多点试验、生产试验与示范中的表现,结果表明:2年的生产示范产量可达61.02 t/hm2,比对照增产11.60%,增加经济效益显著。并对其理化品质分析的表明,蜀萝9号符合加工萝卜和企业深加工的要求,此外还提出了蜀萝9号的栽培技术与制种技术。  相似文献   

甬优9号在三明市的种植表现及关键栽培技术     

郑红卫 《福建农业科技》2012,(7):11-12

甬优9号在三明市试种表现高产稳产、产量潜力大。该文总结了甬优9号在三明市的种植表现,并提出高优关键栽培技术。  相似文献   

双低三系杂交油菜蜀杂9号选育研究   总被引：1，自引：0，他引：1  

王茂林  赵云 《中国油料作物学报》2001,23(4):73-74

蜀杂9号为四川大学生命科学学院育成的甘蓝型油菜细胞雄性不育三系中熟杂交种,参加四川省区试产量为1917．0－2256．0kg/hm^2,比对照种中油821增产33．82％,生产示范最高产量2541．0kg/hm^2。品质优良,芥酸含量0．02％,硫甙（含吲哚硫代葡萄糖甙）含量23．03μmol/g,含油量（干基）41．47％,2001年通过四川省农作物品种审定委员会审定。  相似文献   

Studies on the mechanism of vaccinal immunity to Marek''s disease   总被引：1，自引：0，他引：1  

L.N Payne  P.C Powell  M.C Rennie  L.J.N Ross 《Comparative immunology, microbiology and infectious diseases》1978,1(1-2):31-36

Current knowledge of the nature of the antigens and of the host immune responses in vaccinal immunity to Marek's disease is reviewed. It is suggested that a two-step mechanism of resistance operates. The first step involves humoral and cell-mediated responses directed against viral antigens; the second step occurs after challenge with Marek's disease virus and consists of cellmediated responses directed against tumour cells.  相似文献   

Milk Protein Polymorphism in Kangayam Cattle     

Jeichitra V  Kandasamy N  Panneerselvam S 《Tropical animal health and production》2003,35(2):147-153

This paper reports the milk protein polymorphism, the allele frequencies of variants and the possible linkages among various combinations of milk protein phenotypes in the Kangayam cattle of south India. Milk samples from 156 Kangayam cows were typed by starch gel and polyacrylamide gel electrophoresis for caseins and whey proteins, respectively. All the four milk protein components studied, 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">_s1-casein, 9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-casein, 9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-lactoglobulin and 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin, exhibited polymorphism with high allele frequencies of 0.9231±0.0151 for 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">_s1-casein C, 0.9263±0.0148 for 9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-casein A, 0.9135±0.0159 for 9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-lactoglobulin B and a relatively high frequency of 0.6218±0.0275 for 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin A. The mean heterozygosity estimated over all the four milk protein loci was 0.2420. Genetic equilibrium was observed among all the loci studied, except 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin. Linkage analysis confirmed the non-independence between 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">_s1- and 9w4550174220k7w/xxlarge946.gif" alt="beta" align="MIDDLE" BORDER="0">-caseins and between caseins and 9w4550174220k7w/xxlarge945.gif" alt="agr" align="BASELINE" BORDER="0">-lactalbumin phenotypes.  相似文献   

New approaches in vaccine development   总被引：4，自引：0，他引：4  

Leclerc C 《Comparative immunology, microbiology and infectious diseases》2003,26(5-6):329-341

In the last century, vaccines have been one of the most powerful tools for preventing infectious diseases. Smallpox has been eradicated and other diseases such as poliomyelitis or measles have been reduced to very low levels in many regions of the world. However, infectious diseases remain the leading cause of death worldwide. Thus, the development of vaccines to prevent diseases for which no vaccine currently exists such as AIDS or malaria as well as the improvement of efficacy and safety of existing vaccines remains a high priority. Achieving such ambitious goals in a near future will certainly require a strong modification of the methods that have been used so far to identify vaccine candidates. In particular, modern vaccinology could strongly benefit of the latest developments of molecular biology and immunology. Here, we will discuss some potential applications of the increasing knowledge of pathogen genomes as well as the immune system for the discovery of new antigenic targets and the development of new strategies of vaccination.  相似文献   

Medical management of biological warfare and bioterrorism: place of the immunoprevention and the immunotherapy     

Binder P  Attre O  Boutin JP  Cavallo JD  Debord T  Jouan A  Vidal D 《Comparative immunology, microbiology and infectious diseases》2003,26(5-6):401-421

Biological weapons are considered as mass destruction and terror weapons. Terrorism including bioterrorism is the major threat in the future conflicts for our nations. The aim of bioterrorism is more related to the potential disorganisation of the society than to the lethal effects of the agents used. The dramatic consequences cannot be discarded, especially if contagious agents such viral are used. The preparation of specific defence measures is a major challenge for our countries.

The knowledge acquired from the struggle against natural infectious diseases and recent events are essential to improve behaviours to face the biological weapon threats. The defence attitude is based on the anticipation of the threat, the management of the victims, and the restoration of the operational capabilities. This global defence attitude implies six important functions: (i) alert, (ii) detection and diagnosis, (iii) availability of pharmaceutical countermeasures such as vaccine, sera and anti-infectious medicine and products, (iv) medical management of victims, (v) training and information, (vi) research and development.

Passive and active immunoprevention and immuntherapy belong to the approaches discussed in the context of bioterrorism countermeasures. Further researches might be focused on these topics.  相似文献