Characterization of the horseweed (Conyza canadensis) transcriptome using GS‐FLX 454 pyrosequencing and its application for expression analysis of candidate non‐target herbicide resistance genes     

Yanhui Peng  Laura LG Abercrombie  Joshua S Yuan  Chance W Riggins  R Douglas Sammons  Patrick J Tranel  C Neal Stewart Jr 《Pest management science》2010,66(10):1053-1062

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家蚕糖转运蛋白基因BmST3的克隆及序列分析与表达研究   总被引：1，自引：0，他引：1  

孔卫青  杨金宏 《蚕业科学》2010,36(4):604-609

糖转运蛋白在家蚕体内糖类化合物的转运与代谢过程中发挥重要作用。在电子克隆的基础上,通过RT-PCR方法克隆了家蚕糖转运蛋白基因BmST3(GenBank登录号:GQ871756)。生物信息学分析结果显示,该基因位于家蚕27号染色体,开放阅读框(ORF)长1434bp,编码477个氨基酸,预测蛋白有典型的Sugar_tr结构域和12个疏水的跨膜结构域,与埃及伊蚊、致倦库蚊、冈比亚按蚊、赤拟谷盗登录号分别为EAT47626、EDS35465、EAA11457、EFA05337的同源蛋白相似性均在60%以上。RT-PCR检测和基因芯片信号值分析结果显示,BmST3基因的表达具有组织特异性,在家蚕5龄第3天幼虫的9种组织中,主要在马氏管中大量表达,推测其可能在马氏管细胞膜内外物质的跨膜转运中发挥作用。  相似文献   

植物吸收和转运磷素的分子机理研究进展     

孙传范  肖凯  韩胜芳  赵芳华  鲍金香 《中国农业科技导报》2011,13(2):17-24

磷素是植物必需的大量营养元素,植物对磷素的吸收和植物体内磷素的再度转运是位于细胞原生质膜上的磷转运蛋白介导的需能主动运输过程。概述了植物吸收和转运磷素的生理特征,植物磷转运蛋白(PT)的结构、功能和PT及磷调节子Pho基因的表达调控机制,以及突变体技术在植物磷吸收、转运中的研究进展和应用前景。旨在为今后在分子水平上进一步揭示植物对磷素吸收和转运的生物学机制提供理论依据。  相似文献   

稻瘟病菌ABC转运蛋白基因中 SSR的分布及其功能预测     

刘林  李成云  杨静  苏源  吴晗  徐玲  胡燕玲 《植物病理学报》2011,41(4):371-378

 ABC转运蛋白在真菌的致病性方面起着重要的作用。本研究查找和分析了稻瘟病菌基因组中47个ABC转运蛋白基因的外显子区、内含子区、5′-UTR和3′-UTR区中的SSR,并对编码区中SSR的扩张或收缩对蛋白结构的影响进行了预测。结果表明,SSR在这些基因的调控区和编码区分布不均一,且在基因的外显子区、内含子区、5′-UTR和3′-UTR区中SSR的组成和分布均不相同;基因的编码区中三碱基和六碱基SSR相对较多,SSR的基序大都表现为GC含量较高,编码的亲水性氨基酸出现频率远远高于疏水性氨基酸。根据稻瘟病菌自然群体中SSR的变化幅度,预测了SSR的扩张或收缩对蛋白二级结构的影响,发现SSR的扩张或收缩都可能对蛋白的结构产生影响。暗示着SSR的变异在致病相关基因的变异中可能扮演着重要角色。  相似文献   

A molecular dynamics simulation on the self-assembly of ABC triblock copolymers. 3. Effects of block composition in asymmetric triblock copolymers     

Min?Jae?Ko  Seung?Hyun?Kim  Won?Ho?JoEmail author 《Fibers and Polymers》2003,4(1):15-19

The self-assembly of asymmetric ABC triblock copolymers in the ordered structure is investigated using an isothermal-isobaric molecular dynamics simulation. Unlike symmetric ABC triblock copolymers, more fascinating morphologies are observed in asymmetric ones because of a larger difference of incompatibility between the components. Various modes of self-assembly in assymmetric ABC triblock copolymers are also observed depending on the block composition. When the composition of block A is changed from 0.125 to 0.25 at the samef _B =0.25, the morphological transition from the “cylinder in cylinder” to “cylinders at cylinder” structure is observed in the simulation. In the case of ABC triblocks withf _B =0.5, a lamellar-type structure is changed to a cylinder-type structure with increasing the length of block A. When the mid-block length increases further tof _B =0.625, the “spheres on cylinder” structure is observed in both the A₁₀B₅₀C₂₀ and A₂₀B₅₀C₁₀ triblocks. From these results, the phase diagram of ABC triblock copolymers can be constructed. Dedicated to Professor Won-Jei Cho on the occasion of his retirement.  相似文献   

烟草钾转运体基因NtHAK1的克隆及表达模式分析   总被引：3，自引：0，他引：3  

鲁黎明  杨铁钊 《核农学报》2011,25(3):469-476

采用RT-PCR的方法,结合3'RACE与5'RACE,从普通烟草(Nicotiana tabacum)品种K326中克隆出烟草K+转运体基因NtHAK1的全长cDNA序列.该序列长度为2016bp,编码378个氨基酸,其分子量为42.27kDa,等电点7.2.生物信息学分析表明,该蛋白具有6个跨膜区,含有K+转运蛋白...  相似文献   

基于AMESim的农用车辆悬架自整定PID调节     

吴飞  刘存香 《农机化研究》2011,33(12)

为了提高农用运输车行驶的平顺性,利用AMESim软件设计了农用车辆前后轮不同悬架弹簧与减振器连接形式、采用或未采用悬架参数自整定PID调节的模型,并根据模型得到了悬架振动位移图。从仿真结果可以看出:采用参数自整定PID调节的农用车辆悬架,无论是乘坐的舒适性,还是对路面的动载,都得到了很好的改善,控制效果显著。  相似文献   

Molecular cloning,tissue distribution and the expression of cystine/glutamate exchanger (xCT,SLC7A11) in different tissues during development in broiler chickens     

《动物营养（英文）》2020,6(1):107-114

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A selectively suppressing amino acid transporter: Sodium-coupled neutral amino acid transporter 2 inhibits cell growth and mammalian target of rapamycin complex 1 pathway in skeletal muscle cells     

Lingyu Zhang  Yehui Duan  Qiuping Guo  Wenlong Wang  Fengna Li 《动物营养（英文）》2020,6(4):513

Sodium-coupled neutral amino acid transporter 2 (SNAT2), also known as solute carrier family 38 member 2 (SLC38A2), is expressed in the skeletal muscle. Our research previously indicated that SNAT2 mRNA expression level in the skeletal muscle was modulated by genotype and dietary protein. The aim of this study was to investigate the key role of the amino acid transporter SNAT2 in muscle cell growth, differentiation, and related signaling pathways via SNAT2 suppression using the inhibitor α-methylaminoisobutyric acid (MeAIB). The results showed that SNAT2 suppression down-regulated both the mRNA and protein expression levels of SNAT2 in C2C12 cells, inhibited cell viability and differentiation of the cell, and regulated the cell distribution in G0/G1 and S phases (P < 0.05). Meanwhile, most of the intercellular amino acid content of the cells after MeAIB co-culturing was significantly lower (P < 0.05). Furthermore, the mRNA expression levels of system L amino acid transporter 1 (LAT1), silent information regulator 1, and peroxisome proliferator-activated receptor-gamma co-activator 1 alpha, as well as the protein expression levels of amino acid transporters LAT1 and vacuolar protein sorting 34, were all down-regulated. The phosphorylated protein expression levels of mammalian target of rapamycin (mTOR), regulatory-associated protein of mTOR, 4E binding protein 1, and ribosomal protein S6 kinase 1 after MeAIB treatment were also significantly down-regulated (P < 0.05), which could contribute to the importance of SNAT2 in amino acid transportation and skeletal muscle cell sensing. In conclusion, SNAT2 suppression inhibited C2C12 cell growth and differentiation, as well as the availability of free amino acids. Although the mTOR complex 1 signaling pathway was found to be involved, its response to different nutrients requires further study.  相似文献   

Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron     

Khurram Bashir  Yasuhiro Ishimaru  Hugo Shimo  Yusuke Kakei  Takeshi Senoura  Ryuichi Takahashi 《Soil Science and Plant Nutrition》2013,59(6):803-812

Iron (Fe) is an essential micronutrient for plants; however, despite its abundance in soils it is not readily available in neutral to alkaline soils. Plants secrete phenolics, such as protocatechuic acid (PCA) and caffeic acid (CA), to absorb and utilize precipitated apoplasmic Fe from root surfaces. However, the synthesis and secretion of phenolics have not been well characterized in plants. We have identified and characterized a rice (Oryza sativa L.) mutant with reduced amounts of PCA and CA in xylem sap and root exudates; hence we named it phenolics efflux zero 2 (pez2). PEZ2 localized to the plasma membrane in onion (Allium cepa L.) epidermal cells and transported PCA when expressed in Xenopus laevis oocytes. PEZ2 expression was observed in whole root near root tips. Similarly, strong expression was observed in leaves. In line with reduced amounts of PCA and CA in xylem sap, the xylem Fe concentration was also low in pez2 plants. These results suggest that PEZ2 is involved in solubilization of apoplasmic Fe in rice.  相似文献