Application of fungicides to reduce yield loss in anthracnose-infected lupins     

G.J. Thomas  M.W. Sweetingham  K.G. Adcock 《Crop Protection》2008,27(7):1071-1077

A range of fungicides were evaluated for control of anthracnose (Colletotrichum lupini) in lupin. Glasshouse investigation identified fungicides that reduced disease severity with varying degrees of efficacy. When applied 1 d prior to infection, azoxystrobin, chlorothalonil, mancozeb and copper oxychloride fungicides were highly effective. Systemic fungicides such as tebuconazole, benomyl and carbendazim were less effective. Application timing was important; fungicides such as chlorothalonil and mancozeb were less effective when applied 5 d prior to infection compared with 1 d prior. Application after infection was ineffective for all fungicides. In field experiments application of azoxystrobin, mancozeb or chlorothalonil during flowering and podding reduced incidence of anthracnose infection on pods. Yield responses occurred in moderately susceptible, moderately resistant or resistant cultivars. Seed infection was reduced but not eradicated. Application of foliar fungicides for anthracnose control is potentially a viable management option for lupin production in high anthracnose risk areas of Western Australia.  相似文献   

5种药剂对油茶炭疽病的防治效果研究   总被引：2，自引：0，他引：2  

邓鑫州  黄连桂  邓荫伟 《安徽农业科学》2011,39(16):9653-9654

[目的]研究5种药剂对油茶炭疽病的防治效果,以筛选出能有效防治油茶炭疽病的杀菌剂。[方法]测定了1%波尔多液、50%多菌灵500倍液、75%百菌清600倍液、80%代森锰锌700倍液和70%甲基硫菌灵800倍液对油茶炭疽病的防治效果。[结果]1%波尔多液和80%代森锰锌的防治效果达75.00%,50%多菌灵和75%百菌清的防效为69.14%;70%甲基硫菌灵的防效欠佳,仅为14.81%,与其他4种药剂存在极显著差异;1%波尔多液喷施后,药剂在叶片上的附着期长,防治效果最好。[结论]1%波尔多液是防治油茶炭疽病的最佳方案。  相似文献   

切花红掌炭疽病和叶霉病的病原鉴定     

李丽  ;刘会香  ;郭先锋  ;孟凡志 《山东农业科学》2014,(6):111-115

红掌炭疽病和叶霉病是危害切花红掌生产的两种重要病害。本研究通过传统形态学结合分子生物学方法对切花红掌生产中两种主要病害进行鉴定,并确定病原菌的分类地位。从感病植株上通过常规组织分离法获得病原菌株,对其进行形态学观察和致病性测定,通过ITS序列分析和比对,对其进行分子鉴定。结果表明:红掌炭疽病的致病菌为Colletotrichum gloeosporioides,与GenBank中查到的登录号为KC172072.1的ITS序列同源性最高,同源性大于99%;红掌叶霉病系复合侵染,其致病菌为Cladosporium cladosporioides和Alternaria alternata,分别与GenBank中查到的登录号为KC880082.1和JX406531.1的ITS序列同源性最高,同源性分别为96%和97%。分子生物学鉴定与形态学鉴定结果一致。  相似文献   

白菜炭疽病苗期抗病性鉴定方法的研究   总被引：7，自引：0，他引：7  

刘爱媛 《中国蔬菜》1997,1(1):2-0

根据不同的接种方法、苗龄、温度、接种物浓度及调查时期对白菜炭疽病苗期抗病性表现的影响，并与成株期抗病性比较，筛选出一套快速、准确反映成株期抗病性的苗期抗病性鉴定方法。即在２０～３２℃温度下，用６０００－１２０００个／ｍＬ分生孢子的悬浮液于子叶期点滴接种，保湿２４～４８小时，当感病品种的病情指数达８５左右时调查各供试品种的病情指数。  相似文献   

Phenolic compounds as defence response of pepper fruits to Colletotrichum coccodes     

《Physiological and Molecular Plant Pathology》2013

Qualitative and quantitative changes of individual and total phenolics induced by Colletotrichum coccodes fungal infection have been studied in two susceptible sweet pepper cultivars ‘Soroksari’ and ‘Bagoly’, and the role of soluble phenolic compounds in plant's defence mechanism has been evaluated. Three distinct parts were analysed on pepper fruit: healthy tissue, anthracnose lesion, and bordering tissue, and individual phenolic compounds have been identified with the use of HPLC-MS system. In pepper fruit pericarp 21 phenolic compounds have been determined; the prevalent apigenin, quercetin and luteolin glycosides, chlorogenic acid and one chrysoeriol glucoside. C. coccodes infection increased the accumulation of chlorogenic acid, chrysoeriol glucoside, quercetin and luteolin glycosides in infected bordering tissue of both analysed pepper cultivars compared to healthy pepper tissue or symptomatic spot. Total apigenin derivatives did not show a significant increase in bordering tissue compared to the healthy pepper fruit in contrast to other groups of phenolics. This suggests a lesser role of apigenin glycosides in pepper plant defence against the Colletotrichum fungus. Intense phenolic synthesis was characteristic for the bordering zone between the healthy and infected plant tissue resulting in higher total phenolic content which might hinder the fungus to spread from the infected cells into the healthy tissue.  相似文献   

高锰酸钾防治柑桔疮痂病、炭疽病的应用试验     

林健全 《福建林业科技》2001,(Z1)

柑桔疮痂病、炭疽病不仅影响果树的正常生长 ,而且影响果实的外观、产量、品质 ,降低经济效益。应用不同药品对柑桔疮痂病、炭疽病防治效果试验表明 ,高锰酸钾 6 0 0倍溶液防治效果理想 ,并能减少果实农药的残留量 ,可值得推广  相似文献   

Isolation of resistance gene analogs from grapevine resistant and susceptible to downy mildew and anthracnose   总被引：1，自引：0，他引：1  

W. Seehalak  S. Moonsom  P. Metheenukul  P. Tantasawat 《Scientia Horticulturae》2011

Downy mildew and anthracnose are major diseases of the grapevine (Vitis spp.) cultivars grown in Thailand. Due to the deleterious effects of fungicides frequently used for disease management in grapevine, disease resistance genes have been sought after with the ultimate goal of developing new cultivars with improved disease resistance levels. In this study, nucleotide-binding site (NBS)-leucine rich repeat (LRR) class of resistance gene analogs (RGAs) were cloned by PCR amplification using degenerate primers specific to P-loop and GLPL, conserved regions of NBS. Ninety-one clones containing putative RGA sequences were obtained from a downy mildew and anthracnose resistant hybrid ‘NY88.0507.01’ and a susceptible cultivar ‘Black Queen’. These cloned sequences were subdivided into 14 groups based on their nucleotide sequence similarity of 90% or greater. BLASTx of fourteen selected clones showed the highest amino acid sequence similarity with known NBS-LRR proteins or putative resistance (R) protein candidates. Multiple alignments of these representative RGAs with 5 known R proteins revealed conserved P-loop, kinase-2, RNBS and GLPL motifs which are typical components of the NBS-LRR proteins. Four RGAs had at least 40% identity with known R proteins. Phylogenetic analysis demonstrated that the representative RGAs from both resistant and susceptible grapevines dispersed along the phylogram on the two major branches of either TIR (Drosophila Toll and mammalian Interleukin-1 Receptor) or non-TIR type of the NBS-LRR proteins.  相似文献   

藏红花球茎腐烂病的调查及初步分析     

张国辉  龙安辉  贺定翔 《勤云标准版测试》2007,(6)

藏红花在贵州省种植历史很短,关于病害的发生危害和防治的研究也较少。目前,藏红花球茎腐烂病造成了较大的危害,成为种植方面的首要病害。该文通过对藏红花进行实地病害调查和组织分离法分离病原菌,并进行培养和鉴定,实验结果表明,藏红花球茎腐烂病是由炭疽菌和镰刀菌交互侵染导致的结果。  相似文献   

利用分子标记定位普通菜豆抗炭疽病基因     

陈明丽  王兰芬  王晓鸣  张晓艳  王述民 《作物学报》2011,37(12):2130-2135

为了定位中国普通菜豆的抗炭疽病基因, 选取抗炭疽病地方品种红芸豆(国家库编号F2322)与高感菜豆品种京豆(国家库编号F0777)配制杂交组合, 构建F₂抗感分离群体和F_2:3家系, 用菜豆炭疽菌81号生理小种鉴定抗病性并分析遗传性。结果表明, 红芸豆对菜豆炭疽菌81号小种的抗性是由一显性单基因控制的, 暂将该基因命名为Co-F2322。用分离群体分组分析法(BSA)和SSR、CAPs分子标记技术, 将该基因定位在B1连锁群上, 利用软件Mapmaker 3.0和Mapchart 3.0计算标记与目的基因间的遗传距离, 检测到3个SSR标记BMc32、C871、Pvm98和2个CAPs标记g1224、g683与抗炭疽病基因连锁, 遗传距离分别为26.06、3.58、13.56、3.81和12.75 cM。  相似文献   

Identification of Specific RAPD Markers Linked to Anthracnose Resistant Gene in Native Wild Grapes of China     

WANG Xi-ping  WANG Yue-jin  ZHOU Peng  ZHENG Xue-qin 《中国农业科学(英文版)》2001,(1):35-39

Randomly amplified polymorphic DNA (RAPD) was employed to detect molecular markers linked to anthracnose ( Spheceloma ampelinum de Bary) resistant gene in the native wild grapes ( Vitis L. ) of China. RAPD marker OPJ13-300 was linked to anthracnose resistant gene using 90-3 cross F1 V. quinquangularis Rehd (shang-24) × V. vinifera (Longyan). The marker was verified in 90-3 cross F1, Chinese wild grapes and V. riparia and European grape cuitivars. This work has provided a solid basis for molecular marker-assisted selection (MAS) to disease resistance and cloning of disease resistant genes.  相似文献