74种鸟类线粒体基因组碱基组成及特征分析   总被引：3，自引：0，他引：3  

高英凯  苗永旺  苏小茜  池振奋  俞赟  姜枫 《云南农业大学学报(自然科学版)》2009,24(1):51-58

 在鸟类线粒体基因组中,不同物种的线粒体碱基组成和特性存在明显的差异。截至2008年5月,GenBank细胞器基因组资源数据库共公布了74种鸟类线粒体全基因组数据。本研究利用已公布的鸟类线粒体基因组全序列分析其碱基组成特征。结果表明：(1)鸟类线粒体基因组密码子第二位的GC含量值波动范围十分狭窄,而密码子第三位的GC含量值波动范围很大。(2) 密码子第三位碱基中C的含量波动范围较大,为32.60%-50.70%。(3)线粒体基因组GC含量主要由密码子第三位的碱基C和T的变化引起。(4)密码子第三位碱基的GC含量与线粒体基因组的GC含量的变化存在相关性。这些结果,为今后鸟类线粒体基因组的深入研究提供借鉴和参考资料。  相似文献   

白羽肉鸭高致病性禽流感疫苗免疫效果研究     

卫龙兴  俞财荣  王福泉  戴莲群 《现代农业科技》2009,(21):263-264

对白羽肉鸭高致病性禽流感疫苗免疫效果进行了试验研究,结果表明,白羽肉鸭如能按照免疫程序规范实施免疫操作,能够获得较好的免疫效果,首免剂量以0．5-0．8mL为宜。  相似文献   

野鸭源H3N8亚型禽流感病毒NS基因的分子克隆及序列分析     

郭亮  柴洪亮  张兰兰  张进  侯志军  孙颖  魏韬  华育平 《东北林业大学学报》2009,37(7)

根据GeneBank上已发表的H3N8亚型禽流感的非结构蛋白基因(NS)序列,设计了一对特异性引物,成功地从A/mallard/SanJiang/167/2006(H3N8)中克隆到NS基因序列,该克隆基因全长860 bp,编码NS1和NS2两种非结构蛋白.与其他H3N8亚型的NS基因进行了同源性比较,核苷酸同源性为65.5%～98.3%,推导的氨基酸序列同源性为65.4%～97.8%.NS基因遗传进化树形成两个分支,该分离株处于等位基因B组.另外,此毒株NS基因在263～277位没有发生15个碱基的缺失.A/mallard/SanJiang/167/2006(H3N8)株149位为丙氨酸,其不能感染哺乳动物.  相似文献   

湖南省岳阳市鸡源性大肠杆菌血清型鉴定   总被引：2，自引：0，他引：2  

李先磊  吕点点  刘立超  何计峰 《安徽农业科学》2009,37(26):12574-12574

从湖南省岳阳市周边8个养鸡场收集病料,从中分离出169株大肠埃希氏菌;血清型鉴定结果表明,岳阳市周边鸡源性大肠杆菌的主要血清型为：O78（41株）、O1（35株）、O2（22株）、O55（16株）,共占总待检菌株的67．4％,占定型菌株的80．9％。  相似文献   

禽流感扑杀补偿政策的市价补偿标准问题探析   总被引：2，自引：0，他引：2  

梅付春 《河南农业科学》2011,40(4):30-33

扑杀是政府应对禽流感疫情的有效应急措施,对扑杀的禽只给以补偿是对禽主因扑杀而遭受损失的必要补救.补偿标准的高低直接影响着禽主配合政府扑杀行动的意愿和积极性.在剖析现行补偿标准缺陷的基础上,分析了市价补偿标准的必要性和可行性,并提出了相应的对策和建议.  相似文献   

禽流感（AI）的研究     

陈磊 《商界．城乡致富》2011,(1):53-57

禽流感（Avian Influenza,AI）是由甲型流感病毒的一种亚型引起的禽类传染病,是危害世界养禽业的主要疫病之一。介绍了禽流感的发病症状、流行趋势及病原特征,并就禽流感的诊断技术、防治手段、免疫等方面的研究进展进行了综述。  相似文献   

Turnover of Village Chickens Undermines Vaccine Coverage to Control HPAI H5N1          下载免费PDF全文

J. P. Villanueva‐Cabezas  P. T. Campbell  J. M. McCaw  P. A. Durr  J. McVernon 《Zoonoses and public health》2017,64(1):53-62

Highly pathogenic avian influenza (HPAI) subtype H5N1 remains an enzootic disease of village chickens in Indonesia, posing ongoing risk at the animal–human interface. Previous modelling showed that the fast natural turnover of chicken populations might undermine herd immunity after vaccination, although actual details of how this effect applies to Indonesia's village chicken population have not been determined. We explored the turnover effect in Indonesia's scavenging and mixed populations of village chickens using an extended Leslie matrix model parameterized with data collected from village chicken flocks in Java region, Indonesia. Population dynamics were simulated for 208 weeks; the turnover effect was simulated for 16 weeks after vaccination in two ‘best case’ scenarios, where the whole population (scenario 1), or birds aged over 14 days (scenario 2), were vaccinated. We found that the scavenging and mixed populations have different productive traits. When steady‐state dynamics are reached, both populations are dominated by females (54.5%), and ‘growers’ and ‘chicks’ represent the most abundant age stages with 39% and 38% in the scavenging, and 60% and 25% in the mixed population, respectively. Simulations showed that the population turnover might reduce the herd immunity below the critical threshold that prevents the re‐emergence of HPAI H5N1 4–8 weeks (scavenging) and 6–9 weeks (mixed population) after vaccination in scenario 1, and 2–6 weeks (scavenging) and 4–7 weeks (mixed population) after vaccination in scenario 2. In conclusion, we found that Indonesia's village chicken population does not have a unique underlying population dynamic and therefore, different turnover effects on herd immunity may be expected after vaccination; nonetheless, our simulations carried out in best case scenarios highlight the limitations of current vaccine technologies to control HPAI H5N1. This suggests that the improvements and complementary strategies are necessary and must be explored.  相似文献   

Pathogenecity of Avian Influenza Virus H5N2： A Report from Western Iran     

A. M. Bahrami  A. Delpisheh  H. Mirzaei  A. Haeri 《农业科学与技术》2011,(2):211-216

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骆岗机场鸟类多样性及鸟击风险分析   总被引：2，自引：0，他引：2  

王丽君  周立志  万政云  张保卫  许仁鑫  张有瑜  薛委委 《野生动物》2010,31(3):127-130

2007年10月～2008年9月,采用样线调查法对骆岗机场及周边地区不同生境的鸟类进行了调查,根据鸟类相对数量、遇见频率、生境利用率和飞行高度,对机场鸟类鸟击风险进行评价。调查共记录到鸟类84种,其中留鸟26种,夏候鸟26种（留鸟和夏候鸟有3种重叠）,冬候鸟20种,旅鸟15种。繁殖鸟中东洋型27种,古北型20种,广布种2种。在机场外围,农田-鱼塘生境鸟类密度最高。机场围栏附近各生境鸟类密度均高于内部草坪。全年中,机场鸟类密度在秋季达到最高。春季优势种为白头鹎,夏季优势种包括麻雀、八哥、灰椋鸟和家燕,秋季优势种为麻雀和云雀,冬季优势种为黑水鸡。鸟击风险评价结果表明,白头鹎、麻雀、小白鹭、云雀、家燕等11种鸟类鸟击风险最高。建议采取生态环境治理与人工干扰相结合的方法防范鸟击。  相似文献   

DNA vaccine encoding avian influenza virus H5 and Esat-6 of Mycobacterium tuberculosis improved antibody responses against AIV in chickens     

Sara Oveissi  Abdul Rahman Omar  Khatijah Yusoff  Fatemeh Jahanshiri  Sharifah Syed Hassan 《Comparative immunology, microbiology and infectious diseases》2010,33(6):491-503

The H5 gene of avian influenza virus (AIV) strain A/chicken/Malaysia/5744/2004(H5N1) was cloned into pcDNA3.1 vector, and Esat-6 gene of Mycobacterium tuberculosis was fused into downstream of the H5 gene as a genetic adjuvant for DNA vaccine candidates. The antibody level against AIV was measured using enzyme-linked immunosorbent assay (ELISA) and haemagglutination inhibition (HI) test. Sera obtained from specific-pathogen-free chickens immunized with pcDNA3.1/H5 and pcDNA3.1/H5/Esat-6 demonstrated antibody responses as early as 2 weeks after the first immunization. Furthermore, the overall HI antibody titer in chickens immunized with pcDNA3.1/H5/Esat-6 was higher compared to the chickens immunized with pcDNA3.1/H5 (p < 0.05). The results suggested that Esat-6 gene of M. tuberculosis is a potential genetic adjuvant for the development of effective H5 DNA vaccine in chickens.  相似文献