利用RAPD标记鉴定和区分梅花42个宫粉型品种   总被引：12，自引：0，他引：12  

张俊卫  柴玉荣  包满珠 《园艺学报》2004,31(4):487-490

 利用RAPD标记，对形态特征相似的42个宫粉型梅花品种的基因组DNA进行随机引物PCR扩增。从120个引物中筛选出21个引物，共扩增出96条谱带，其中多态性谱带68条，占扩增谱带数的70．8％ ，品种问相似系数范围为0．737～0．961。在21个引物中，7个引物扩增的谱带可区分27个品种，7个引物相互组合可区分其余的l5个品种。梅花品种RAPD指纹图谱的建立可以为品种知识产权保护提供依据。  相似文献   

利用分子生物学技术鉴别真、假杂交稻的研究   总被引：7，自引：2，他引：5  

SCIENTIA AGRICULTURA SINCA 《中国农业科学》1999,32(2):8-97

对由湖北省某公证处封存的两份杂交稻材料进行DNA、蛋白质指纹鉴定,首先采用业已建立的可特异鉴定以明恢63为父本的杂交稻真、假的P18引物进行RAPD分析,发现两份材料的叶片及种子DNA在0.8kb扩增带上存在着有和无的区别;而所用的42个水稻微卫星标记,发现5个水稻微卫星标记具有多态性;在此基础上,进一步对两样品的种子水溶性蛋白质毛细管电泳图谱进行比较,二者在出峰时间及峰值上存在差异。上述结果表明,两份材料并非同一杂交组合,但在遗传背景上存在较大的相似性。  相似文献   

42个杂交粳稻亲本SSR指纹图谱的构建及遗传多样性分析     

刘海珍  李莉  滕斌  从夕汉  陆徐忠  倪大虎  宋丰顺  汪秀峰  杨剑波 《安徽农业科学》2009,37(10):4420-4423

[目的]研究利用SSR技术构建以杂交粳稻亲本为主的SSR指纹图谱。[方法]用48对SSR引物扩增了42个杂交粳稻亲本的基因组DNA,从中筛选出12对具有稳定多态性的引物,推荐为核心引物。建立了42个杂交粳稻亲本×12个核心引物的DNA指纹数据库。[结果]采用类平均法聚类分析计算42个基因型的相似系数,在相似系数0.73处,可将42个杂交粳稻亲本分为5大类。[结论]聚类分析的结果基本反映了品种间的亲缘关系。  相似文献   

甜樱桃品种SSR指纹图谱数据库的建立   总被引：6，自引：0，他引：6  

艾呈祥  张力思  魏海蓉  苑克俊  金松南  刘庆忠 《中国农学通报》2007,23(5):55-55

为了建立甜樱桃品种指纹图谱数据库,从而对不同甜樱桃品种进行分子鉴定,以“红灯”、“萨米脱”、“吉塞拉5号”、“吉塞拉6号”等24个甜樱桃主要栽培品种为试材,利用2.5%琼脂糖凝胶电泳进行SSR引物的筛选,选择差异明显且等位基因数目少（2个）的SSR标记,从38对SSR引物中筛选出能够扩增出稳定带型且不同材料之间差异明显的SSR引物,并从中选出10对SSR引物进行甜樱桃分子指纹分析,根据各甜樱桃DNA样品的电泳条带结果进行赋值,利用Visual Basic和Access软件进行数据库编程,创建一个数据库,入选的10对引物对样品的扩增结果赋值排列起来,即成为甜樱桃的“基因身份证”号码,从而根据分子身份证对不同甜樱桃种质进行鉴别。  相似文献   

Source apportionment of lake bed sediments to watersheds in an Upper Mississippi basin using a chemical mass balance method   总被引：1，自引：0，他引：1  

D. W. Kelley  E. A. Nater 《CATENA》2000,41(4):393

Sediment samples were collected from three known sources contributing to a depositional basin of the Upper Mississippi River, USA. The heavy silt fraction (2–63 μm, density >2.95) isolated from the sediments was analyzed for 42 elements by inductively coupled plasma-mass spectrophotometry (ICP-MS) to give signature profiles characteristic of each source catchment. Known proportions of heavy silt fractions from each source were mixed and the composite samples analyzed in the same way. The proportions of components in the composite samples were compared to their apportionments calculated statistically using the US Environmental Protection Agency (USEPA) chemical mass balance (CMB) air quality receptor model. This showed that the CMB technique can apportion sediments to their catchment sources reliably. The technique was then applied to dated sediments of Lake Pepin, in the Upper Mississippi basin, to apportion them to their contributing catchments. The results show that almost all the recent sediments deposited in the basin originated in the Minnesota River catchment, and that the sediment load increased over time as anthropogenic impacts increased. This technique could be used elsewhere to determine sediment provenance and identify changes in sediment sources over time.  相似文献   

3个玉米杂交种和亲本SSR指纹图谱的构建   总被引：47，自引：0，他引：47  

吴渝生  杨文鹏  郑用琏 《作物学报》2003,29(4):496-500

以3个云南省大面积推广的玉米杂交种及其亲本为试材,从96对SSR引物中筛选出24对引物,这些引物分布在玉米基因组的10条染色体上,每对引物可以检测到1～6个数目不等的多态性片段(allele),共97个多态性片段,平均为4.04个,片段大小介于85～300 bp之间.在比较分析各试材图谱的基础上,探讨了构建品种指纹图谱的统计学方法,建立了适  相似文献   

20个花生品种的SSR标记指纹图谱构建     

刘冠明  郑奕雄  黎国良 《勤云标准版测试》2006,(6)

为了保护农民以及育种者的利益,发展稳定可靠的品种鉴定方法具有重要意义。对南方花生主产区20个品种进行了SSR标记指纹图谱研究,从64对SSR标记引物中筛选出4对构建了20个花生品种的指纹图谱,该图谱能使19个品种相互区分。  相似文献   

Genetic diversity among rattan genotypes from India based on RAPD-marker analysis     

Prabalee Sarmah  P. K. Barua  R. N. Sarma  P. Sen  P. C. Deka 《Genetic Resources and Crop Evolution》2007,54(3):593-600

Southeast Asia hosts a great diversity of different rattan genotypes. There are 5 genera and 60 different species of rattan in India and the Northeastern region is a natural sanctuary for 4 different genera and 16 different species. The natural reserves of this species have come under the threat of genetic erosion due to overexploitation. This investigation was directed at characterizing 15 rattan genotypes of the genera Calamus, Plectocomia and Daemonorops which yield rattans of commercial importance, based on RAPD fingerprints. From 20 different random decamer primers, 12 primers gave reproducible amplification profiles and 104 polymorphic bands. A considerable degree of polymorphism (98.1%) was detected among the genotypes. Jaccard’s coefficient of similarity ranged from 0.146 to 0.632 with a mean of 0.320±0.082, signifying extensive genetic divergence among the genotypes studied. UPGMA cluster analysis clearly distinguished P. assamica Griff. and C. erectus Roxb. The other 13 genotypes were grouped into two distinct clusters, one cluster involving two C. tenuis genotypes along with C. inermis T.Anders. and C. acanthospathus Griff. and the other cluster involving the rest of the Calamus genotypes along with D. jenkinsianus (Griff.) Mart. Unique fingerprints for 7 Calamus and 1 Daemonorops genotypes were detected. The results presented in this paper demonstrated the utility of RAPD markers in elucidating patterns of genetic variation among genotypes of the three main rattan genera of Northeast India and in identifying individual genotypes, which may serve as potential sources of unique genetic material for genetic improvement and conservation.  相似文献   

玉米SSR指纹技术反应体系的优化   总被引：9，自引：0，他引：9       下载免费PDF全文

孔祥彬  张春庆 《玉米科学》2006,14(2):027-029

SSR指纹技术是一种非常有效的遗传分析技术,在生物遗传多样性分析、遗传图谱构建、品种鉴定和品种保护等方面有着广泛的用途。SSR技术简单、重复性高、采用银染显色技术等优点,为SSR的广泛应用奠定了良好的基础和可行性。在SSR扩增过程中,影响因素很多,如Mg2 、dNTPs、Taqpolymeras、引物等。本实验通过对各种影响因素不同浓度梯度的比较,得出优化后的SSR扩增的反应体系为:20μL的反应体积中包括2.0mmol/LMg2 ,0.1mmol/LdNTPs,0.25μmol/L引物,0.5UTaqDNA聚合酶,10×PCRbuffer2.0μL和40ng模板DNA。扩增条件为94℃预变性5min,94℃1min,60℃2min,72℃2min,35个循环,最后72℃5min。  相似文献   

Denaturing Gradient Gel Electrophoresis (DGGE) as a tool for the characterisation of Brachionus sp. strains     

Stefania Dooms  Spiros Papakostas  Stefan Hoffman  Daan Delbare  Alexander Triantafyllidis  Olav Vadstein  Patrick Sorgeloos 《Aquaculture (Amsterdam, Netherlands)》2007,262(1):29-40

Many zooplanktonic organisms, like the cyclic parthenogenetic rotifer Brachionus plicatilis (Rotifera: Monogononta), are actually a complex of species and biotypes with a high degree of morphological similarity (i.e. cryptic species). Various phylogenetic studies with molecular markers (e.g. ITS1 and COI) on wild Brachionus populations described the presence of at least nine genetically divergent Brachionus species and biotypes. Because different studies found evidence that these cryptic species and biotypes differ significantly in ecological preferences and thus probably behave differently in response to rearing conditions in the hatchery, questions rise on the actual identity of the rotifer strains used in aquaculture, where Brachionus discrimination is still based on morphology. This study is a part of an investigation of the genetic make-up of strains used in hatcheries, aquaculture research institutes and laboratories, and describes the rapid and sensitive PCR-DGGE method for the detection of Brachionus species and biotypes based on nucleotide sequence variation within the mitochondrial 16S rRNA gene. Considerable genetic diversity was found, albeit smaller within hatcheries than within laboratories and aquaculture research institutes. All 16S haplotypes produced an unambiguous DGGE fingerprint out of which a database was constructed.  相似文献