北碚榕扦插繁殖技术研究北碚榕扦插繁殖技术研究   总被引：3，自引：0，他引：3  

齐亮  李先源  李名杨 《安徽农业科学》2010,38(6):2906-2907

[目的]得出北培榕扦插繁殖技术最优的生根处理方式。[方法]研究了激素种类、处理浓度、处理时间、插穗长度以及基质种类对北培榕扦插生根的影响。[结果]插穗的最优长度为10 cm,用1 000 mg/L的IBA溶液处理15 s生根率可达96.7%。总体来看,IBA处理插穗的效果比NAA要好,根系较发达;扦插基质以珍珠岩：腐殖土为2∶1最好。[结论]扦插成活的难易程度除了和植物本身特性有关,还与处理插穗的激素种类、浓度、时间等有关。插穗的长度、扦插季节以及枝条的粗细对插穗生根也有很大影响。  相似文献   

3种生长素对蓝叶忍冬枝条扦插生根的影响   总被引：3，自引：0，他引：3  

朱永超  李彬  廖伟彪 《草业科学》2016,33(1):61-66

以蓝叶忍冬(Lonicera korolkowi‘Zabclii’)为材料,研究了2种不同浓度(50、100 mg·L-1)的生长素吲哚乙酸(IAA)、吲哚丁酸(IBA)和萘乙酸(NAA)在不同处理时间(30、60 min),对蓝叶忍冬生根部位、根数和根长的影响。结果表明,3种生长素对蓝叶忍冬枝条扦插生根都产生了一定的影响,IAA处理增加蓝叶忍冬的生根率和根数,但抑制根的伸长。NAA处理对蓝叶忍冬生根效果不明显,仅对根长有一定的促进作用。与对照相比,50 mg·L-1IBA处理60min显著增加了蓝叶忍冬的生根率和根数(P0.05)。另外,3种生长素对蓝叶忍冬枝条生根部位也有影响,3种生长素处理都可以增加皮层生根数,对切口愈伤组织的生根有一定的抑制作用。其中IBA处理对生根部位影响的效果最明显,100 mg·L-1处理30 min可以显著提高皮层生根数并抑制愈伤组织生根(P0.05)。综合比较3种生长素促进生根的效果,IBA最好,IAA和NAA次之。  相似文献   

Micropropagation of gooseberry, Ribes grossularia     

Margareta Welander 《Scientia Horticulturae》1985,26(3):267-272

A method for rapid micropropagation of gooseberry (Ribes grossularia cultivar ‘Hinnonmäki Yellow’) was established. The optimum concentration for shoot production was obtained at 2.2 μM BAP and 0.05 μM IBA with MS nutrient medium. The Lepoivre salt medium with no hormones was used for elongation of shoot clusters, and in this medium about 50% of the shoots formed roots. For both rooted and unrooted shoots, 100% survival was obtained after transfer to soil. It was possible to store shoots in vitro for 130 days without any sub-culture and with 100% survival if the temperature was increased successively after cold storage.  相似文献   

Embryogenesis and plant regeneration from anther culture in loquat (Eriobotrya japonica L.)     

Junqiang Li  Yongqing Wang  Lihua Lin  Lijun Zhou  Nan Luo  Qunxian Deng  Junren Xian  Chunxia Hou  Yuan Qiu 《Scientia Horticulturae》2008

The object of this study was to induce embryogenesis and establish plant regeneration system for anther culture in loquat (Eriobotrya japonica L.). Cold pretreatment was a key factor, and supplement of 2,4-D in the media was absolutely necessary for induction of calluses from cultured loquat anthers. The best response of anthers to in vitro culture was obtained when a 48-h cold pretreatment was employed to flower buds at 4 °C in darkness. Genotype was a decisive factor for embryo differentiation. When anther-derived calluses of three loquat cultivars, i.e., cv. ‘Longquan1’, ‘Dawuxing’ and ‘Zaozhong6’, were transferred to embryo differentiation medium, embryos were induced only for cv. ‘Dawuxing’ on MS medium containing 3% sucrose, 0.23 μM ZT in combination with 0.05 μM NAA + 0.05 μM IBA or 0.11 μM NAA + 0.10 μM IBA, and the differentiation rates were 3.33% and 10.00%, respectively. The results of histological studies showed that embryos developed through typical globular, heart, torpedo and cotyledon stages after 4 weeks of culture. The treatment designed to mature the embryos on medium containing 3% of sucrose at 4 °C under darkness for 4 weeks was effective for subsequent embryo germination and plant conversion, which gave rise to 72.5% plant recovery. Cytological studies showed that 26 plantlets were haploids (n = 17) and the remaining 4 plantlets were diploids for the 30 regenerants tested.  相似文献   

In vitro tetraploid induction and generation of tetraploids from mixoploids in Astragalus membranaceus     

Lan-Lan Chen  Shan-Lin Gao 《Scientia Horticulturae》2007

This paper describes an efficient colchicine-mediated technique for the in vitro induction of tetraploids in Astragalus membranaceus and its confirmation by flow cytometry. Buds immersed in 0.2% colchicine solution for 36 h prior to culture induced as high as 35.3% tetraploid plants. Colchicine-induced tetraploids remained stable after 6 months in soil. Leaf characteristics of diploids and tetraploids in A. membranaceus were compared. It was determined that leaf sizes of glasshouse-grown plants and stomatal sizes of both in vitro and glasshouse-grown plants were suitable parameters for identifying putative tetraploids in A. membranaceus. As well as generating tetraploids, this technique generated mixoploids in A. membranaceus. Calli derived from mixoploid leaves, were induced to form buds and shoots. Individual shoots were classed as diploid, mixoploid and tetraploid by flow cytometry. This callus-based technique can be employed when a genome-doubling agent generates mixoploids but no tetraploid.  相似文献   

6-BA和IBA浓度对不同基因型大豆胚尖诱导丛生芽的影响     

许诺  张君  王丕武 《大豆科学》2012,31(4):678-679,684

以吉林17,吉林27,吉林28和吉林29共4种大豆基因型胚尖为外植体诱导丛生芽,研究了不同浓度的六苄基嘌呤(6-BA)和吲哚丁酸(IBA)对胚尖再生率的影响.结果表明:不同基因型大豆在不同激素浓度配比下再生率存在明显差异.吉林28和吉林29更适合大豆胚尖再生系统,其最佳激素配比均为2.0 mg·L-16-BA +0.2 mg·L-1IBA,再生率为分别达78.17％和80.04％.  相似文献   

Auxin-impregnated hygroscopic gel: effects on ponderosa pine and common hackberry seedlings     

Gerald A. Tuskani  Patricia L. Ellis 《New Forests》1991,5(4):359-367

Greenhouse and field studies were conducted to determine the effects of indole3-butyric acid (IBA) and 2,4-dichlorophenoxyacetic acid (2,4-D) on root development and survival of 3+0 bareroot Pinus ponderosa (Dougl. ex Laws.) and 2+0 bareroot Celtis occidentalis (L) seedlings. In the greenhouse, 100 and 200 ppm. IBA and 10 and 50 ppm 2,4-D were applied to seedlings through a root dip in an auxin hygroscopic gel mix. A randomized complete block analysis of variance indicated that IBA gel treatments increased root volume and root dry weight in hackberry and had no effect on ponderosa pine; 2,4-D gel treatments had detrimental effects on both species. In the field, IBA gel treated ponderosa pine seedlings exhibited improved height and survival relative to the non-treated control seedlings. There was no detectable improvement in survival in the field for hackberry. However, diameter and stem dry weight were lower for hackberry seedlings treated with the gel dip alone.  相似文献   

吲哚丁酸对一串红种子萌发及幼苗生长的影响     

孙睿  邵红  王仲  李修平 《林业科技》2012,37(3):6-7,30

不同浓度吲哚丁酸(IBA)对一串红种子萌发及幼苗生长影响的研究结果表明:10-6 mg/L的IBA可以增加一串红种子的发芽率、侧根数、叶绿素含量和根系活力。  相似文献   

MS培养基和IBA对糯米香茶水培生根的影响   总被引：2，自引：0，他引：2  

张翠玲  谭乐和  陈鹏  刘向阳  孙燕  庄辉发  林兴军 《热带农业科学》2012,32(12):23-26,32

采用不同浓度MS培养基与吲哚丁酸(IBA)的组合对糯米香茶进行水培,通过测定糯米香茶的生根能力,探讨MS培养基与IBA对水培苗的影响。结果表明:低浓度的MS培养基和IBA组合A1B1(1/4 MS和0.1 mg/LIBA)对糯米香茶水培苗生根数、根长、根鲜重和生根率的促进作用明显优于对照,有利于糯米香茶的水培生根,而高浓度的MS培养基和IBA组合则对糯米香茶的水培生根具有抑制作用。本研究为缩短水培糯米香茶的育苗周期、提高糯米香茶无性系生根能力提供理论依据。  相似文献   

Efficient Micropropagation of Lilium Oriental Hybrid 'Starfighter' through Secondary Multiplication and Improvement of Survival Rate     

JIA Caihong  LIU Juhua  ZHANG Jing  XU Biyu  TAN Guanglan  Jin Zhiqiang 《热带作物学报》2008,29(6)

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