干旱对大豆膜脂过氧化,组织抗氧化的影响     

孙会兰  佟代言 《河北农业大学学报》1991,14(4):37-41

在水分胁迫下,大豆幼苗叶片相对含水量、随胁迫加强而降低,质膜透性,膜脂过氧化水平,组织自动氧化速率,随胁迫加强而增加。上述指标与大豆品种抗旱能力有关,对干旱敏感的品种变化幅度大。保护酶活性随胁迫加强而增加。抗旱性越强,增加幅度越大,在水分胁迫下,膜透性、膜质过氧化水平与组织自动氧化速率,保护酶活性之间存在着一定相关性。  相似文献   

水分胁迫下苹果属植物叶片叶绿素降解的膜脂过氧化损伤作用   总被引：18，自引：0，他引：18  

曹慧  韩振海  许雪峰 《中国农业科学》2003,36(10):1191-1195

 苹果属植物幼苗在水分胁迫下,随着胁迫时间的延长,叶片叶绿素(Chl)降解加剧,活性氧(如O_2~、H_2O_2)、膜脂过氧化产物(如MDA)含量明显增加,细胞质膜相对透性(PMP)明显增大。其中,Chl、O_2~、H_2O_2、MDA、PMP的变化幅度表现为平邑甜茶大于新疆野苹果。经相关分析表明,Chl含量与O_2~、H_2O_2和MDA的含量,及PMP之间均呈显著负相关。V_E、抗坏血酸(ASA)和甘露醇预处理可延缓水分胁迫诱导的MDA含量的产生和减慢Chl降解;而Fe~(2+)、H_2O_2及Fenton反应则可刺激MDA含量的增加,加速Chl降解。  相似文献   

多元醇对油菜衰老的生理调控及增产作用探讨   总被引：5，自引：0，他引：5  

周伟军  奚海福 《中国农业科学》1995,28(3):8-13

 研究了不同时期喷施多元醇对甘蓝型油菜的生理效应及增产效果。结果表明：初花期喷施多元醇能显著提高油菜叶片叶绿素含量和光合速率，减少脂质过氧化产物丙二醛积累和乙烯释放量，使叶片超氧物歧化酶与过氧化氢酶活性下降变慢；多元醇能增强油菜根系活力，使植株稳健生长；多元醇还能改善油菜的经济性状，增加有效分枝数和每株角果数，初花期和盛花期喷施多元醇分别增产10.4%和7.2%，对油菜籽的品质无不良影响，对油菜生育期影响不大。  相似文献   

机械伤害和茉莉酸对豌豆幼苗膜脂过氧化的影响   总被引：10，自引：0，他引：10  

刘艳  郝燕燕  刘艳艳  黄卫东 《中国农业科学》2005,38(2):388-393

 切割后离体芽苗极易受病菌侵染而发生腐烂变质，严重影响着当前芽菜采后运输和销售。为此,本试验以豌豆芽苗为试材,研究了机械伤害和外施不同浓度（±）茉莉酸（JA）处理对豌豆幼苗膜脂过氧化的影响。结果表明，伤害和1～100 μmol·L-1 JA处理可以显著提高苯丙氨酸解氨酶（PAL）和多酚氧化酶（PPO）活性，同时使活性氧代谢加强，O2-·产生速率和H2O2含量迅速增加，相应的抗氧化酶（SOD、CAT、APX、POD）活性提高。适宜浓度JA（1～10 μmol·L-1）处理可有效诱导防御反应产生，同时，通过提高抗氧化酶活性使膜脂过氧化程度减轻；高浓度JA（100 μmol·L-1）处理造成植物活性氧代谢失调，膜脂过氧化加剧。综合分析认为，10 μmol·L-1JA是诱导豌豆幼苗防御反应产生的适宜处理浓度。  相似文献   

油桃裂果与膜脂过氧化的关系   总被引：5，自引：0，他引：5  

丁勤  韩明玉  田玉命 《西北农业学报》2004,13(4):200-202,206

以华光油桃为试材，研究了油桃裂果与果实生长发育过程中超氧物自由基积累和膜脂过氧化的关系。结果表明：果实着色期，果实内的超氧物自由基积累，SOD活性因受抑制而下降，MDA含量上升，细胞膜受伤害而透性增大，此时为裂果最严重时期；随后裂果率下降，SOD活性上升，MDA含量下降，膜透性降低，细胞逐渐修复；在接近采收时SOD活性下降，MDA含量再次上升。这除和成熟有关外还与因降雨、灌溉等引起的裂果有关。果实发育过程中SOD活性与MDA含量、细胞膜透性呈负相关，SOD活性高的果实不易裂果。  相似文献   

茶多酚和L-EGCG的抗烟致脂质过氧化作用   总被引：2，自引：0，他引：2  

沈生荣  杨贤强  杨法军  忻文娟 《浙江大学学报(农业与生命科学版)》1992,(Z1)

从茶叶中提取的天然多酚类化合物茶多酚和L-表没食子儿茶素没食子酸酯(L-EGCG)对由香烟气相物质诱发的大鼠肺细胞的脂质过氧化有很强的抑制作用,且复合体茶多酚及其主要单体L-EGCG抑制效果接近,同时两者对超氧阴离子自由基和羟自由基都有十分明显的清除作用,表明茶多酚与L-EGCG的抗脂质过氧化机理之一是有效地清除了脂质过氧化的启动子氧自由基.  相似文献   

Biochemical and histopathological studies to assess chronic toxicity of triazophos in blood, liver and brain tissue of rats     

Smita JainRafat S. Ahmed  Vinod Kumar AroraBasu Dev Banerjee 《Pesticide biochemistry and physiology》2011,100(2):182-186

Triazophos, O,O-diethyl-1-H-1,2,4-triazol-3-yl phosphorothioate, (TZ) is an organophosphorus pesticide which is extensively used in agriculture for controlling insect pests. Except a FAO/WHO report no study has investigated its short-term toxicity with respect to its potential to cause biochemical and histopathological alterations. The present study was designed to identify the effect of TZ at different doses (1.64, 3.2 and 8.2 mg/kg) on the oxidative stress parameters in blood as well as organs involved in xenobiotic metabolism (liver and brain) following chronic exposure for 90 days. Moreover, the study also delineates the effect of TZ on the histo-architecture of these organs. The results indicated a dose dependent induction (p < 0.001) of oxidative stress, as evident by increased malondialdehyde (MDA) level and compromised antioxidant defense including glutathione S transferase (GST) activity, glutathione (GSH) content and ferric reducing ability of plasma (FRAP) in blood, and increased MDA level with concomitantly decreased GSH content in tissues, following chronic exposure to TZ. The ratio of MDA: FRAP in blood was found to be increased following chronic exposure to TZ and may serve as a suitable indicator of severity of oxidative damage. Onset of such biochemical alterations is one of the early adaptive responses to TZ exposure which leads to histopathological alterations in terms of diffuse fatty changes expanding from mid-zonal area to whole lobule in liver. However, increased oxidative stress did not bring any morphological alteration in brain. The present study concludes that induction of oxidative stress, leading to subsequent histopathological alterations in liver, is an important mechanism underlying the TZ induced chronic toxicity.  相似文献   

Exogenously applied hexaconazole ameliorates salinity stress by inducing an antioxidant defense system in Brassica napus L. plants     

Gholam Ali Akbari  Mostafa Hojati  Seyed Ali Mohammad Modarres-Sanavy  Faezeh Ghanati 《Pesticide biochemistry and physiology》2011,100(3):244-250

The ability of hexaconazole (HEX) to ameliorate salinity stress was studied in canola plants (Brassica napus L.). Canola seedlings were subjected to sodium chloride (NaCl) treatment. A treatment with 200 mM NaCl reduced growth parameters, chlorophyll content and protein content as well as increased the proline (Pro) content in canola plants. In addition, NaCl stress increased the endogenous, nonenzymatic antioxidants and the activity of antioxidant enzymes, such as peroxidase (POX; EC 1.11.1.7), superoxide dismutase (SOD; EC 1.15.1.1) and catalase (CAT; EC 1.11.1.6). When these plants were treated with a combination of NaCl and 50 mg L⁻¹ HEX, the inhibitory effects of NaCl stress were decreased by increasing the root growth, shoot growth, dry weight (DW), chlorophyll content, protein content and antioxidant enzyme activity by ameliorating the salinity injury. These results suggested that HEX has an important role in the enhancement of plant antioxidant systems and resistance to salinity in canola plants.  相似文献   

静电场对草莓体内超氧化物歧化酶和脂质过氧化影响的研究     

李玉花  刘福全 《沈阳农业大学学报》1993,24(2):137-141

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Using lipid analysis and hyphal length to quantify AM and saprotrophic fungal abundance along a soil chronosequence     

T.C. Balser  K.K. Treseder 《Soil biology & biochemistry》2005,37(3):601-604

We evaluate the use of signature fatty acids and direct hyphal counts as tools to detect and quantify arbuscular mycorrhizal (AM) and saprotrophic fungal (SF) biomass in three Hawaiian soils along a natural soil fertility gradient. Phospholipids16:1ω5c and 18:2ω6,9c were used as an index of AM and saprotrophic fungal biomass, respectively. Both phospholipid analysis and hyphal length indicated that the biomass of AMF was greatest at the highest fertility site, and lowest where phosphorus limits plant growth. Saprotrophic fungal biomass did not vary. Hyphal length counts appeared to under-estimate SF abundance, while the phospholipid AMF:SF ratio was in line with expectations. This study indicates that phospholipids may be a valuable and reliable tool for studying the abundance, distribution, and interactions between AM and saprotrophic fungi in soil.  相似文献