Acute‐Phase Proteins,Oxidative Stress and Enzyme Activities of Blood Serum and Peritoneal Fluid in Cattle with Abomasal Displacement     

M. Maden  A.S. Ozturk  A. Bulbul  G.E. Avci  E. Yazar 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2012,26(6):1470-1475

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Status of Lipid Peroxidation, Antioxidants, and Oxidation Products of Nitric Oxide in Equine Babesiosis: Status of Antioxidant and Oxidant in Equine Babesiosis     

Serdar Deger PhD  VMD  Prof. Dr.  Yeter Deger PhD  VMD  Assoc. Prof. Dr.  Kamile Bicek PhD  VMD  Assoc. Prof. Dr.  Nalan Ozdal PhD  VMD  Asst. Prof. Dr.  Abdurrahman Gul PhD  VMD  Assoc. Prof. Dr. 《Journal of Equine Veterinary Science》2009,29(10):743-747

Equine babesiosis is a tick-borne protozoal disease of horses caused by Theileria equi and Babesia caballi. The disease is endemic in most tropical and subtropical areas. The aim of this paper is to assess the antioxidant status, lipid peroxidation, and oxidation products of nitric oxide (NO) in horses and mules naturally infected with T. equi and B. caballi. East and Southeast Anatolian horses and mules living in rural region of the Eastern border of Turkey were used as the material for this study. These animals are used as pack animal (3–7 years of age). Infected animals were in acute or subacute infection period. In the current study, malondialdehyde (MDA), oxidation products of NO (nitrate and nitrite), serum glutathione (GSH), vitamin E, and retinol levels were analyzed in 58 equids (horse and mule) infected with T. equi and B. caballi as well as in 44 healthy equids. Compared with controls, the level of MDA and nitrate increased significantly (P < .01, P < .05, respectively), whereas GSH concentration and levels of vitamin E decreased significantly (P < .01). There was no significant change in the level of nitrite and retinol between two groups. The results of the current study suggest that in equids infected with T. equi and B. caballi, this alteration in the lipid peroxidation, oxidants, and antioxidants may be related to the host's defenses against parasitic infection and may play a central role in the pathologic conditions associated with babesiosis.  相似文献   

The effects of organophosphate insecticide diazinon on malondialdehyde levels and myocardial cells in rat heart tissue and protective role of vitamin E     

Ayse Ogutcu  Suna Kalender  Fatma Bayrakdar 《Pesticide biochemistry and physiology》2006,86(2):93-98

Diazinon is an organophosphate insecticide has been used in agriculture and domestic for several years. Vitamin E (200 mg/kg, twice a week), diazinon (10 mg/kg, per day), and vitamin E (200 mg/kg, twice a week)+diazinon (10 mg/kg, per day) combination was given to rats orally via gavage for 7 weeks. Body and heart weights, malondialdehyde (MDA) level in heart tissue and ultrastructural changes in myocardial cells were investigated at the end of the 1st, 4th, and 7th weeks comparatively with control group. When diazinon-treated group was compared to control group body and heart weights were decreased significantly at the end of the 4th and 7th weeks. It was observed that, at the end of 1st, 4th, and 7th weeks there was a statistically significant increase in MDA levels when diazinon- and vitamin E +diazinon-treated groups were compared to control group. While at the end of the 1st week statistically significant changes were not being observed, at the end of 4th and 7th weeks statistically significant decrease was detected in MDA levels when vitamin E+diazinon-treated group was compared to diazinon-treated group. In our electron microscopic investigations, while vacuolization and swelling of mitochondria myocardial cells of diazinon-treated rats were being observed, swelling of several mitochondria were observed in vitamin E+diazinon-treated rats. We conclude that vitamin E reduces diazinon cardiotoxicity, but vitamin E does not protect completely.  相似文献   

Effect of tongxinluo capsule on endothelial function in stable angina pectoris patients     

QIAN Xiao-xian  CHEN Yan-ming  LIU Yong  ZHOU Bin  CHEN Lin  WU Wei-kang 《园艺学报》2006,22(9):1698-1701

AIM:To investigate clinical effect of tongxinluo capsule in treating stable angina pectoris patients,and its influence on endothelial function,superoxide dismutase (SOD) and malondialdehyde (MDA).METHODS:One hundred and twenty-four stable angina pectoris patients were divided into three groups,isosorbide treatment group (41 cases),tongxinluo capsule treatment group (40 cases),tongxinluo and isosorbide treatment group (combined treatment group,43 cases).The serum concentrations of nitric oxide (NO),endothelin-1 (ET-1),SOD and MDA were determined before and after treatment.The data in traetment groups were compared with that in normal control.RESULTS:The symptoms of 3 groups were significantly improved,and the total effective rate of tongxinluo capsule group and combined treatment groups were better than that in isororbide treatment group (85.00% and 88.37% vs 73.17%,P<0.05).Before treatment,the levels of serum NO and activity of SOD in angina patients were lower than that in control group.The serum MDA and ET-1 levels were higher than those in control.The levels of serum NO and SOD activity were increased remarkably after tongxinluo capsule or tongxinluo combined treatment.However,besides the concentration of NO increased after isosorbide treatment,the levels of serum ET-1 and MDA and SOD activity were not changed.CONCLUSIONS:The results suggest that tongxinluo capsule could effectively improve the symptoms of stable angina pectoris,and it is important for tongxinluo capsule to increase NO level and decrease ET-1 product,scavenge free radical and prevent lipid peroxidation.  相似文献   

Effect of cholecystokinin-octapeptide on focal cerebral ischemia/reperfusion injury in rats     

YANG Shi-fang  LING Yi-ling  LING Yi-qun  DUAN Guo-chen  WANG Jian-qin 《园艺学报》2003,19(4):472-476

AIM:To examine the effect of cholecystokinin-octapeptide (CCK-8) on focal cerebral ischemia/reperfusion injury and its underlying mechanisms.METHODS:By using the suture model of focal cerebral ischemia and reperfusion, the effects of intracerebroventricular (icv) injection of CCK-8 and proglumide, nonselective CCK receptors antagonist, on the infarct size, regional cerebral blood flow (rCBF), and the levels of nitric oxide (NO), malondialdehyde (MDA) were observed in different brain regions of rats subjected to 1 h focal cerebral ischemia followed by 24 h reperfusion.RESULTS:(1) pretreatment with different doses of CCK-8 (0.3 μg,1.0 μg,2.0 μg or 4.0 μg) could attenuate the infarct size, but the statistically significant effects of CCK-8 were obtained only at the doses of 1.0 μg and 2.0 μg(P＜0.05). The neuroprotective effects of CCK-8 were blocked by pretreatment with proglumide. Administration of proglumide alone could worsen the ischemia/reperfusion injury. (2) CCK-8 (1.0 μg) inhibited the increase in NO, MDA levels in the ischemic core, and also inhibited the increase in NO level in the ischemic penumbra. The rCBF in the CCK-8 group was significantly higher than the normal value at 24 h after reperfusion (P＜0.05).CONCLUSIONS:These results suggest that both endogenous and exogenous CCK-8 alleviate focal cerebral ischemia/reperfusion injury. Such an action may be associated with inhibition of free radical-induced injuries and the improvement in rCBF.  相似文献   

干旱及复水处理对抗旱性不同小麦品种/系苗期生理生化指标的影响     

张平  张慧  刘俊娜  环秀菊  王倩朝  李莉  刘永江  覃鹏 《西北农业学报》2020,30(12):1795-1802

为明确干旱胁迫及复水处理对抗旱性不同小麦材料生理指标的影响,以抗旱型品种‘靖麦12号’与干旱敏感型品系‘皮麦’为材料,以正常水分管理作为对照,待小麦长至三叶期,采用干旱胁迫-复水法,取幼苗叶片测量过氧化氢酶、过氧化物酶、超氧化物歧化酶、丙二醛、脯氨酸、脱落酸、总抗氧化能力、可溶性蛋白、可溶性糖以及叶绿素等生理指标的变化情况。结果表明：抗旱型品种‘靖麦12号’与干旱敏感型品系‘皮麦’的保护酶活性、总抗氧化能力、可溶性糖及叶绿素含量均存在显著差异及极显著差异;干旱胁迫下,保护酶活性、脯氨酸、丙二醛、脱落酸、总抗氧化能力均呈上升趋势,可溶性糖、可溶性蛋白及叶绿素含量却明显降低;复水以后,保护酶活性、脯氨酸、总抗氧化能力、可溶性蛋白、可溶性糖含量明显降低,叶绿素、脱落酸、丙二醛含量明显上升;两品种/系的处理与对照间脱落酸、丙二醛含量不存在明显差异。在干旱胁迫和复水情况下,‘靖麦12号’表现出较高的酶活性和较高渗透调节物质且叶绿素含量下降缓慢,因此‘靖麦12号’具有较高的抵御干旱胁迫以及复水后的恢复能力,可作为云南地区重要的抗旱种质资源。  相似文献   

Anti-oxidant status in embryonic,post-hatch and larval stages of Asian seabass (<Emphasis Type="Italic">Lates calcarifer</Emphasis>)   总被引：1，自引：0，他引：1  

Kalaimani N  Chakravarthy N  Shanmugham R  Thirunavukkarasu AR  Alavandi SV  Santiago TC 《Fish physiology and biochemistry》2008,34(2):151-158

The concentrations of anti-oxidant enzymes such as superoxide dismutase (SOD), catalase (CAT) and selenium-dependent glutathione peroxidase (SeGPx), and low molecular weight free-radical scavengers such as reduced glutathione (GSH) and ascorbic acid (vitamin C) were evaluated during the period from gastrulation (GS) to 25 days post-hatch (dph) in the larvae of Asian Seabass, Lates calcarifer. Oxidative damage due to lipid peroxidation (LPO) was also assessed, by evaluation of the formation of malondialdehyde (MDA). All the three anti-oxidant enzymes, SOD, CAT and GPx, showed high activities during gastrulation, suggesting an increased metabolic rate during the period of embryonic development. Though the SOD activity apparently decreased progressively during 3–20 dph of larval development, the difference was not significant. CAT showed high activity during gastrulation and remained constant up to 3 dph, suggesting an increased need to metabolise hydrogen peroxide (H₂O₂) and organic peroxides. In contrast, SeGPx activity increased progressively from 5 dph to 25 dph during larval development, indicating an increased need to detoxify lipid peroxides. This is evident from the observation of increased lipid peroxidation from 10 dph to 25 dph during larval development. GSH levels were low at gastrulation, indicating increased metabolic rate and formation of lipid radicals during this period, corresponding to the decrease in the level of ascorbic acid, which is consumed for regeneration of GSH.  相似文献   

APS对感染CDV犬血常规及抗氧化能力的影响     

陆桂平  张斌  贺生中  翟晓虎  李锦强 《安徽农业科学》2010,38(21):11148-11149,11172

[目的]探讨黄芪多糖（APS）对感染犬瘟热病毒（CDV）犬血常规及抗氧化能力的影响。[方法]选择45d健康、未接种犬瘟热疫苗的幼犬19只,随机分为5组,其中Ⅰ组为空白对照组,Ⅱ组为人工染毒阳性对照组,III组为APS治疗组,IV组为APS与常规治疗结合组,V组为常规治疗组。自人工染毒当日进行治疗,连续治疗5d。各组分别于人工感染前1d及感染后第3、6天于前臂头静脉采血,进行血常规、血清SOD活性和MDA含量测定。[结果]经CDV感染后,血液中RBC、WBC、GRA、Hb含量及SOD活性均降低,MDA含量增加,均显著低于对照组（P〈0.05）。经治疗的各组中,APS和常规治疗对RBC、WBC、GRA、Hb含量及SOD活性均有不同程度的改善,但以APS和常规治疗相结合效果最明显,但与空白对照组仍存在显著差异（P〈0.05）。[结论]APS和常规治疗均可提高人工感染CDV犬的抗氧化能力,但以中西药结合效果较好。  相似文献   

低温胁迫对青杨叶片O^—2,MDA,膜透性,叶水势及保护酶的影响   总被引：13，自引：0，他引：13  

孙昌祖  刘家琪 《内蒙古林学院学报》1998,20(3):32-36

对青杨叶片进行不同时间的零上低温（２℃±１℃）胁迫处理，研究其中Ｏ＝２产生速率、ＳＯＤ和ＣＡＴ活性水平和ＭＤＡ含量的相关变化，并相应测定与之相伴随发生的膜透性和叶水势的改变。结果表明，青杨叶片的Ｏ＝２产生速率随低温胁强的增强而提高，当胁强增大到一定范围时，Ｏ＝２产生速率又趋下降。ＭＤＡ的含量变化趋势相似；ＳＯＤ和ＣＡＴ活性水平也与Ｏ＝２的变化相一致；细胞质膜透性的增大和叶水势的降低则均与低温胁强的提高呈正相关。综观上述结果，证明青杨叶细胞的低温胁迫伤害系由Ｏ＝２诱发的膜脂过氧化，破坏了细胞膜系统所致。  相似文献   

Methyl parathion induced nephrotoxicity in male rats and protective role of vitamins C and E     

Suna Kalender  Yusuf Kalender  Ayse Ogutcu  Bekir Sitki Cevrimli 《Pesticide biochemistry and physiology》2007,88(2):213-218

Methyl parathion is an organophosphate insecticide that has been used in agriculture and domestic for several years. Vitamin C (200 mg/kg bw per day) + vitamin E (200 mg/kg bw per day), methyl parathion (0.28 mg/kg bw per day) and vitamin C (200 mg/kg bw per day) + vitamin E (200 mg/kg bw per day) + methyl parathion (0.28 mg/kg bw per day) combination were given to rats orally via gavage for 7 weeks. Body and kidney weights, malondialdehyde (MDA) levels and histopathological changes were investigated at the end of 4th and 7th weeks comparatively with control group. When methyl parathion-treated group and vitamins C and E + methyl parathion-treated group were compared to control group body and kidney weights decreased significantly at the end of 4th and 7th weeks. MDA levels increased in kidney tissues of the methyl parathion- and vitamins C and E + methyl parathion-treated groups compared to control group. MDA levels decreased significantly in vitamins C and E + methyl parathion treated group compared with methyl parathion treated group at the end of 4th and 7th weeks. In our light microscopic investigations, after 4 weeks of methyl parathion exposure, glomerular atrophy and vascular dilatation, and after 7 weeks, necrosis and edema were observed in the kidney tissues. After 4 weeks of vitamins C and E + methyl parathion exposure, mononuclear cell infiltrations, and after 7 weeks, calcification were detected in the kidney tissues.  相似文献