李果实发育过程中果皮色素、糖和总酚含量及多酚氧化酶活性的变化   总被引：10，自引：2，他引：10  

张元慧  关军锋  杨建民  赵树堂 《果树学报》2004,21(1):17-20

大石早生、龙园秋李、黑宝石、安哥诺4个李品种在果实发育过程中,果皮叶绿素、类胡萝卜素、总酚以及类黄酮含量呈下降趋势,可溶性总糖、蔗糖、果糖、葡萄糖、花青苷含量及多酚氧化酶(PPO)活性逐渐增加。除安哥诺的蔗糖、葡萄糖外,果皮花青苷与不同种类糖含量之间呈显著正相关或极显著正相关,大石早生蔗糖含量与花青苷含量呈极显著正相关,相关系数为0.9688,龙园秋李、黑宝石则呈显著正相关,相关系数分别为0.9481、0.9447;黑宝石、安哥诺果糖与花青苷含量呈显著正相关,相关系数分别为0.9358、0.8925,大石早生、龙园秋李则呈极显著正相关,相关系数分别为0.9438、0.9763;大石早生、龙园秋李、黑宝石果皮葡萄糖与花青苷含量呈显著正相关,相关系数分别为0.8940、0.9022、0.9424。龙园秋李、黑宝石这2个品种果皮总酚含量与PPO活性呈极显著负相关,相关系数分别为-0.9896、-0.9664。  相似文献   

桃、李种间杂交花粉萌发及花粉管行为的观察   总被引：2，自引：1，他引：2  

曹后男  朴日子  宗成文  赵成日  朱波 《果树学报》2004,21(4):302-307

选用3个桃[Prunus persica(L)Batsch]品种和4个李(Prunus salicina Lindl.)品种观察了种间杂交时花粉萌发及花粉管的生长状态。结果表明,李的花粉在桃柱头上的萌发及花粉管的生长都受到很大的阻碍,很少有到达花柱基部或子房上端的花粉管;而桃花粉在李柱头上的萌发及花粉管的生长都很正常,与桃品种间杂交没有差异。即桃和李间存在着单侧种间不亲和现象,而这种单侧种间不亲和现象的原因可能是花柱长度及花粉粒体积的差异。  相似文献   

Variation in total phenolics and antioxidant capacity among European plum genotypes     

H.P. Vasantha Rupasinghe  S. Jayasankar  W. Lay 《Scientia Horticulturae》2006

Our objective was to determine the amount of variation in total phenolics and antioxidants present in European plum fruits, so that it can be utilized in breeding programs to enhance the health benefits. Total antioxidant capacity and total phenolic content of the fruit, and the fruit skin color were determined in 20 genotypes, comprising of released varieties and advanced selections of European plums. Among the 20 genotypes, the total antioxidant capacity ranged from 105 to 424 mg ascorbic acid equivalents (AAE)/100 g fresh weight (FW) while the total phenolic content was 86–413 mg gallic acid equivalents (GAE)/100 g FW. The two parameters had a strong correlation of r² = 0.96. A direct correlation between skin color intensity and total phenolic content could also be observed. This study demonstrates that there is adequate variation in total phenolic compounds and antioxidants within European plums and hence there is potential for improvement towards enhancing these health-promoting phytochemicals in this fruit.  相似文献   

李引选栽培技术研究     

覃守俊  张红艳 《湖北林业科技》1997,(3):13-16

在对国内外李子生产现状调研的基础上，引种优良品种20个，经8a栽培观察，以品种果实经济性状和栽培适应性为目标，选出适宜生产发展的优良早熟品种早黄李和中熟品种芙蓉李，并对栽培技术措施进行了探索。  相似文献   

Production of transgenic cloned pigs expressing the far-red fluorescent protein monomeric Plum     

Masahito WATANABE  Mirina KOBAYASHI  Masaki NAGAYA  Hitomi MATSUNARI  Kazuaki NAKANO  Miki MAEHARA  Gota HAYASHIDA  Shuko TAKAYANAGI  Rieko SAKAI  Kazuhiro UMEYAMA  Nobuyuki WATANABE  Masafumi ONODERA  Hiroshi NAGASHIMA 《The Journal of reproduction and development》2015,61(3):169-177

Monomeric Plum (Plum), a far-red fluorescent protein with photostability and photopermeability, is potentially suitable for in vivo imaging and detection of fluorescence in body tissues. The aim of this study was to generate transgenic cloned pigs exhibiting systemic expression of Plum using somatic cell nuclear transfer (SCNT) technology. Nuclear donor cells for SCNT were obtained by introducing a Plum-expression vector driven by a combination of the cytomegalovirus early enhancer and chicken beta-actin promoter into porcine fetal fibroblasts (PFFs). The cleavage and blastocyst formation rates of reconstructed SCNT embryos were 81.0% (34/42) and 78.6% (33/42), respectively. At 36–37 days of gestation, three fetuses systemically expressing Plum were obtained from one recipient to which 103 SCNT embryos were transferred (3/103, 2.9%). For generation of offspring expressing Plum, rejuvenated PFFs were established from one cloned fetus and used as nuclear donor cells. Four cloned offspring and one stillborn cloned offspring were produced from one recipient to which 117 SCNT embryos were transferred (5/117, 4.3%). All offspring exhibited high levels of Plum fluorescence in blood cells, such as lymphocytes, monocytes and granulocytes. In addition, the skin, heart, kidney, pancreas, liver and spleen also exhibited Plum expression. These observations demonstrated that transfer of the Plum gene did not interfere with the development of porcine SCNT embryos and resulted in the successful generation of transgenic cloned pigs that systemically expressed Plum. This is the first report of the generation and characterization of transgenic cloned pigs expressing the far-red fluorescent protein Plum.  相似文献   

1 MCP处理和贮藏温度对黑宝石李果肉褐变的影响     

邵毅  罗云波  陈安均  卢丞文  朱本忠 《农业机械学报》2010,41(1)

为了探明李果实褐变机理并抑制褐变,研究了1MCP处理和贮藏温度对黑宝石李果肉 冷害褐变、机械伤褐变、多酚氧化酶（PPO）活性、总酚含量和乙烯释放的影响。结果表明 ,黑宝石李果实在（0±05）℃和（3±05）℃条件下分别贮藏75d和60d会发生 果心 的冷害褐变,在（7±05）℃下不会发生冷害褐变,但贮藏期短;机械伤诱导李果实在冷 藏2d后发生近果皮处果肉的褐变症状。5μL/L 1MCP处理李果实12h后,在（ 0±05）℃贮藏时可有效抑制冷害褐变和机械伤褐变,但在（3±05）℃或（7±05） ℃ 时促进果肉褐变。PPO活性和总酚含量的变化与李果实褐变相关,但不是褐变的诱发原因。  相似文献   

青岛国华园鉴湖梅溪水景区的种植设计     

付晓渝  魏胜林  宋亚英 《安徽农业科学》2009,37(20):9761-9762

在突出全园“梅”主题的基础上,根据植物的生态习性及其观赏特点对青岛国华园鉴湖梅溪水景区进行种植设计,营造出丰富的植物空间,以水体的类型进行植物造景,突出湖、溪以及水源三大景区的特点。  相似文献   

乌梅散提取物对兔离体小肠平滑肌运动的影响及机制研究     

尚书凤  王琦  郭素芬  陈志远  王杨科 《中国畜牧兽医》2021,48(2):477-483

本研究旨在探究乌梅散提取物对兔离体小肠平滑肌收缩的作用及其机制。取乌梅30 g、柿蒂50 g、诃子12 g、黄连12 g、郁金12 g,通过煎熬制备乌梅散水提取物储液。兔小肠离体后恒温灌流,利用RM6240多道生物信号采集处理系统进行信息采集和记录。观察乌梅散提取物对离体小肠自发收缩的影响,应用乙酰胆碱（Ach）致使兔小肠平滑肌痉挛性收缩,观察中浓度乌梅散提取物（2.18 g/L）对Ach诱导的收缩的影响,并观察其对Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩的影响。试验结果表明,在药物浓度大于1.09 g/L时,可显著抑制兔离体小肠平滑肌自发收缩的振幅和频率（P<0.05）;Ach可极显著诱导小肠平滑肌收缩的振幅（P<0.01）;2.18 g/L乌梅散提取物可极显著抑制Ach引起的痉挛性收缩振幅和频率（P<0.01）;在无Ca²⁺台氏液中,乌梅散提取物可极显著抑制Ach引起的离体小肠细胞内Ca²⁺和胞外Ca²⁺收缩振幅及频率（P<0.01）。综上所述,乌梅散提取物剂量依赖性地抑制小肠平滑肌自发收缩,可抑制小肠痉挛性收缩,其机制可能与抑制细胞内Ca²⁺释放和胞外Ca²⁺内流有关。  相似文献   

利用流式细胞术鉴定杏及其部分近缘植物的倍性     

杨丽  孙浩元  张俊环  闫爱玲  姜凤超  张美玲  王玉柱 《西北农业学报》2021,(10):1504-1513

果树多倍体生长旺盛、果实大且无籽或少籽、产量高、适应性和抗性强，多倍体砧木能够诱导嫁接植株矮化、提高植株对干旱等非生物胁迫的耐受能力。基于多倍体的诸多优点，发掘、培育与利用多倍体种质资源是果树育种的重要内容。利用流式细胞术鉴定植物倍性技术简单、效率高、结果准确。为了鉴定150 份杏、7份李和9份李杏砧木种质资源的倍性，发掘多倍体资源，采集其幼嫩叶片，利用Partec试剂盒制备细胞核悬液及染色，通过流式细胞仪检测DNA相对含量。以二倍体杏品种''巴斗''为外标，其他种质资源DNA相对含量值与其比较，确定各自倍性水平，若与二倍体''巴斗''DNA相对含量比值近似1，为二倍体；比值近似2，为四倍体；比值近似3，为六倍体。结果表明：150份杏均为二倍体；7份李资源中，2份欧洲李和1份黑刺李为六倍体，4份杏李为二倍体；9份李杏砧木资源中二倍体5份，四倍体1份，六倍体3份。  相似文献   

基于隶属函数法的李涂白防寒措施筛选     

陈蕾  张艳波  崔龙  王雪松  李锋 《分子植物育种》2020,(6):2070-2076

为筛选较适宜的李涂白防寒措施,本研究以‘早红’、‘公主红’和‘吉红’3个李品种为试材,通过不同涂白剂及方法对李树体进行涂白预防冻害,测定枝条冻害级别、植株生长及果实品质等指标,利用正交设计及隶属函数法对防寒效果进行综合评价。结果表明,涂白可降低李树一年生枝冻害情况,促进萌芽及果实生长;筛选出‘公主红’李最佳涂白措施:树干涂白剂+0.5 g/kg纤维素,涂干100 cm。本研究结果可为寒冷地区李树抵御低温胁迫提供技术指导。  相似文献