不同留芽数量对藏红花生长的影响     

李云飞  张正伟  吕志刚 《北方园艺》2010,(2):38-39

研究了不同留芽数量对藏红花生长的影响。结果表明:在整个生长期,不同留芽数量下的藏红花各生长阶段发生的时间差异不大。藏红花种球萌动以后,留3个侧芽的植株生长最快,留2个侧芽的植株生长最慢。在开花期,不同处理的花梗长度、花朵直径、叶片数及叶长差异不显著。随着留芽数量的增多,藏红花的柱头、雄蕊的干重增加,但各处理间没有显著差异。建议在生产中保留2～3个侧芽,以便提高柱头产量。  相似文献   

三个萝卜主产区软腐病病原菌的分离与鉴定   总被引：1，自引：0，他引：1  

邹婧泽  严准  程毅  陈佳  曾粮斌  李智敏  余永廷  高春生 《湖北农业科学》2016,(23):6130-6133

为有效防治萝卜(Raphanus sativus L.)软腐病,提高萝卜产量和质量,从江苏徐州、浙江萧山和湖北利川3个萝卜主产区分别采集萝卜软腐病病样并进行病原菌的分离鉴定及致病力测定。通过结合菌落形态、生理生化指标测定和16S r DNA基因序列分析,确定3株病原菌属于胡萝卜果胶杆菌(Pectobacterium carotovorum)的2个亚种,其中2株为Pectobacterium carotovorum subsp.brasiliense,另1株为Pectobacterium carotovorum subsp.carotovorum。致病力测定分析表明,3株病原菌的致病力存在显著差异,随温度的升高均呈现出增强的趋势。  相似文献   

黄瓜叶片衰老过程中脂滴的原位降解和液泡内降解     

葛姝君  赵丹  周雪  孙晴  刘林 《园艺学报》2016,43(10):2021-2028

对黄瓜不同发育阶段叶片中的脂滴进行超微观察,以期了解衰老叶内脂滴降解的场所。结果表明,衰老叶片中部分质体小球在叶绿体内降解,降解先从小球一侧开始,向另一侧推进。衰老叶片含有胞质脂滴,有些胞质脂滴紧贴叶绿体,显示其发生与叶绿体密切相关;有些胞质脂滴与液泡密切接触,并向液泡内转运,进入液泡后发生降解。显然,黄瓜叶衰老不仅引起脂滴在液泡内降解,也引起脂滴在叶绿体内原位降解,证明液泡并不是脂滴降解的唯一场所。  相似文献   

山黧豆CASase基因的克隆及RNAi载体的构建     

张明科  刘凤娟  陶英杰  胡鑫  李荣硕  徐全乐 《草地学报》2016,24(5):1146-1149

β-腈基丙氨酸合成酶（CASase）是调控山黧豆（Lathyrus sativus）内源毒素β-ODAP合成的关键酶。本研究以山黧豆幼根RNA为模板,利用RT-PCR扩增了505 bp的山黧豆CASase基因序列;通过Gateway BP反应将扩增片段连接到入门载体构建pENTR-CASase。经测序验证后,将目的片段插入到植物表达载体构建pSGRNAi-CASase,并将其转化到农杆菌GV3101中;为进一步的遗传转化奠定了基础。  相似文献   

Phytotoxicity of Cadmium in Radish and Its Effects on Growth,Yield, and Cadmium Uptake     

《Communications in Soil Science and Plant Analysis》2012,43(9):1444-1456

A greenhouse experiment was conducted to study the effects of cadmium (Cd) on growth, biomass yield, and Cd uptake in three radish (Raphanus sativus L.) varieties at the Indian Institute of Horticultural Research, Bangalore, India, during 2008–2009. Plants were subjected to different Cd levels by application of cadmium nitrate [Cd (NO₃)₂] at the rates of 0, 50, 100, and 200 mg Cd kg^?1 soil. Length and fresh and dry biomass yields of shoots and roots decreased because of the phytotoxic effect of Cd. Among three varieties, Japanese White Long showed the greatest sensitivity to Cd toxicity. The accumulation of Cd in shoots and roots was greatest in Japanese White Long, which had greater bioconcentration factor values. Variety Arka Nishanth recorded lower bioconcentration factor values and greater transfer coefficient values, indicating lower Cd accumulation in root tubers in this variety. Hence, variety Arka Nishanth can be preferred in Cd-contaminated soils.  相似文献   

Stimulating and toxicity effects of nickel on growth,yield, and fruit quality of cucumber supplied with different nitrogen sources     

Amir Hossein Khoshgoftarmanesh  Hoda Bahmanziari 《植物养料与土壤学杂志》2012,175(3):474-481

Limited information is available on biological effects of various levels of nickel (Ni) (deficiency to toxicity levels) on growth and yield of certain crops, particularly vegetables. In this sand‐culture study, we investigated the effects of four levels of Ni (0, 50, 100, and 200 μM) on growth, yield, and fruit‐quality attributes of two cucumber cultivars (Cucumis sativus L. cvs. Super Dominus and Negin) supplied with urea or NH₄NO₃ as nitrogen source. Addition of 50 μM Ni to the nutrient solution resulted in a significant increase of shoot and root dry‐matter yield of cv. Negin although this increase was greater in the urea‐fed plants than those fed with NH₄NO₃. In both cultivars, addition of 50 μM Ni increased urease activity and thereby decreased the urea concentration in the urea treatment. Addition of 100 and 200 μM Ni caused a significant decrease in root and shoot growth of cucumber although this decrease was insignificant for cv. Super Dominus in the 100 μM treatment. The highest fruit yield, total soluble solids (TSS), and fruit firmness were achieved at the 50 μM Ni treatment. Regardless of nitrogen source, Ni addition proportional to the concentration used increased leaf Ni concentration and fruit acid ascorbic concentration. The concentration of Ni required for optimum growth and yield of cucumber varied with cultivars. The level of 50 μM was sufficient for optimum growth of cv. Negin in nutrient‐solution culture while lower concentration of Ni was required for cv. Super Dominus. While the beneficial effects of sufficient levels of Ni on growth and yield of urea‐fed plants was greater than with NH₄NO₃‐fed plants, the toxic effects of Ni in these plants were also greater.  相似文献   

The oil content and fatty acid composition of various genotypes of cauliflower,turnip and radish     

K. L. Ahuja  Hari Singh  R. K. Raheja  K. S. Labana 《Plant foods for human nutrition (Dordrecht, Netherlands)》1987,37(1):33-40

Seeds of thirteen genotypes of cauliflower, four of turnip and seven of radish were analysed for oil content and fatty acid composition. Turnip and radish had higher oil content as compared to cauliflower. Oleic, linoleic + eicosenoic and erucic acids were the major fatty acids in the oils of these seeds. Levels of erucic acid were in the order of radish < turnip < cauliflower. Compared to cauliflower and turnip seeds, radish seeds contained higher amounts of palmitic, oleic, and linolenic + eicosenoic acids and lower amounts of linoleic and erucic acids.  相似文献   

黄瓜发芽期耐冷性与赖氨酸脱羧酶基因表达   总被引：5，自引：0，他引：5  

逯明辉  李晓明  陈劲枫  陈龙正  钱春桃 《中国农业科学》2005,38(12):2492-2495

 经15℃低温诱导，采用cDNA-AFLP技术从耐冷性强的黄瓜品种长春密刺中分离到一条特异片段，该片段在耐冷性弱的北京截头中不能被诱导，命名为cctr132。将cctr132回收测序并翻译为氨基酸序列，用blastx和blsatp程序在NCBI GenBank数据库中进行同源性检索和相似性比对，结果发现cctr132与水稻推测的类赖氨酸脱羧酶蛋白同源性为88.37%，相似性为100%，并且在CCTR132中检测到了赖氨酸脱羧酶家族推测的保守结构域PGGXGTXXE，说明黄瓜发芽期耐冷性与赖氨酸脱羧酶基因表达有关。  相似文献   

Effect of micronutrients on B‐N‐Oxalyl l‐α, β‐diamino propionic acid level and its biosynthesis in Lathyrus Sativus     

B.K. Misra  G.K. Barat 《Journal of plant nutrition》2013,36(6):997-1003

The detoxification of L. sativus grains by spraying of 0.5 ppm cobalt (nitrate) and 20 ppm molybdehum (ammonium molybdate) salts at the maximun flowering stage ‐ a suggestion based on preliminary findings ‐ has been confirmed in this investigation. Regulatory mechanisms of these micronutrients at the enzymatic level were also studied. On the basis of these observations, the involvement of a hitherto unknown biosynthetic pathway of BOAA cannot be ruled out.  相似文献   

Iron and zinc absorption characteristics and copper inhibitions in cucurbitaceae     

John M. Swiader 《Journal of plant nutrition》2013,36(10):921-931

Iron and Zn absorption, interactions, and Cu inhibitions were characterized in cucumber (Cucumis sativus L.), watermelon (Citrullus lanatus Thunb.), and pumpkin (Cucurbita moschata Poir.) by kinetic parameters V_max and K_m. Influx and V_max values for Fe and Zn absorption decreased in each species as plant age increased. For the Michaelis constant, K_m, Fe values in cucumber and watermelon and Zn values in watermelon and pumpkin were relatively unchanged with increased plant age. K_m values for Zn absorption in cucumber and Fe absorption in pumpkin decreased as plant age increased. Among species, watermelon appeared to have a particularly effective uptake mechanism for Zn at low solution concentrations. Non‐competitive inhibition of Zn absorption by Fe (20, 50 uM) was indicated in each species. Iron uptake in pumpkin was inhibited non‐competitively by Zn (5, 10 uM), however no significant effects of Zn on Fe absorption were evident in either watermelon or cucumber. Copper (0.5, 1, 5 uM) inhibited uptake of Fe non‐competitively and Zn competitively in each species.  相似文献