氮素对紫花苜蓿根茎叶氮含量及硝酸还原酶活性的影响   总被引：1，自引：0，他引：1  

于铁峰  刘晓静  张晓玲  郝凤 《草原与草坪》2017,(5):14-20

采用营养液砂培方法进行室内盆栽试验,以紫花苜蓿品种甘农3号(Medicago sativa cv.Gannong No.3)和陇东苜蓿(Medicago sativa cv.Longdong)为材料,比较研究了2种氮素形态(铵态氮、硝态氮)和5个氮素水平(0、105、210、315、420 mg/L)对现蕾期紫花苜蓿各部位氮含量及硝酸还原酶活性(NR)的影响。结果表明:2种氮素形态均能显著提高紫花苜蓿根茎叶中硝态氮含量,铵态氮含量、全氮含量及硝酸还原酶活性,且随着氮水平的提高呈先增大后减小的趋势,在210 mg/L处理达峰值。从氮素形态分析,铵态氮肥有利于铵态氮含量的增加,且根茎叶中铵态氮含量、全氮含量在铵态氮210 mg/L处理达到最大值,且显著高于其他处理;硝态氮肥有利于硝态氮含量的增加,且根茎叶中硝态氮含量、硝酸还原酶活性(NR)在硝态氮210 mg/L处理达到最大值,且显著高于其他处理。各部位中,相同氮素形态下,根茎叶中铵态氮含量、硝态氮含量、全氮含量及NR活性均表现为叶片根部茎部,且甘农3号表现优于陇东苜蓿。基于经济效益和生态效益考虑,铵态氮210 mg/L是培养紫花苜蓿较好的形态和水平。  相似文献   

氮素形态对黄瓜幼苗生长及氮代谢酶活性影响   总被引：4，自引：0，他引：4  

师进霖 《中国农学通报》2009,25(22):225-227

用营养液培养的方法,研究了同一氮素水平不同氮素形态比例对黄瓜幼苗生长及氮代谢关键酶：硝酸还原酶（NR）、谷氨酰胺合成酶（GS）活性的影响。结果表明：单独供给硝态氮（NO3^--N）比单独供给铵态氮（NH4^＋-N）更有利于黄瓜幼苗的生长;当NO3^--N∶NH4^＋-N为75∶25时,主根长度、根总表面积、根总体积、株高、茎粗、地上部干重的值最大。NR活性随NO3^--N比例的增加而增加,GS活性在NH4^＋-N比NO3^--N少时,随NH4^＋-N的比例增加而增大,当NO3^--N∶NH4^＋-N为50∶50时活性最大,当NH4^＋-N大于NO3^--N时活性下降。表明NO3^--N∶NH4^＋-N为75∶25时对黄瓜幼苗最适宜。  相似文献   

New isoflavonolignan with quinone reductase inducing activity from Alhagi pseudalhagi (M.B.)     

Ning Li  Guijie Zhang  Yuanjun Xiong  Bolat Makhabel  Xian Li  Xiaoguang Jia 《Fitoterapia》2010

Bioassay-directed separation of the chloroform extracts from the air-dried aerial part of Alhagi pseudalhagi (M.B.) led to the isolation of a new isoflavonolignan (1), together with five known isoflavones (2–6) (Fig. 1). Their structures were identified on the basis of spectral analysis of NMR, MS, IR, UV and CD spectral evidences. The quinone reductase (QR) inducing activities of the extracts and compound 1 were evaluated and the new isoflavonolignan (1) exhibited moderate quinone reductase (QR) inducing activity for hepa lclc7 cells.  相似文献   

植物生长调节剂对大豆功能叶片硝酸还原酶活性的影响   总被引：1，自引：1，他引：0  

洛育孙世臣  李彩凤赵东升 《中国农学通报》2010,26(19):136-139

采用盆栽试验探讨植物生长调节剂G-ABA和S-ABA对大豆品种东农163（高油品种） 和东农42（高蛋白品种）硝酸还原酶活性的影响。结果表明,同一品种不同处理间大豆功能叶片硝酸还原酶活性均表现为单峰变化趋势,其中G-ABA影响顺序为：20 mg/kg＞15 mg/kg＞25 mg/kg＞30 mg/kg＞0 mg/kg＞35 mg/kg,而S-ABA影响顺序为：35 mg/kg＞30 mg/kg＞25 mg/kg＞20 mg/kg＞0 mg/kg＞40 mg/kg;生育期内不同基因型大豆不同处理间功能叶片硝酸还原酶活性（NRA）的变化动态基本相同,表现为中期高,前期和后期低的趋势;但影响效果高蛋白品种明显好于高油品种。  相似文献   

竹子硝酸还原酶活力的研究     

吕成群  何若天 《广西农业生物科学》1990,(4)

对竹子硝酸还原酶活力的研究表明:因竹叶提取液偏酸,用 pH8.5的25mmol/L 磷酸盐缓冲液(含聚乙烯吡略啉酮(PVP)65mg/ml),提取(最终 pH为7.5)的叶片酶活力最高,酶反应达最高速率时的底物 KNO_3浓度为25mmol/L。在漫射光下,用 50mmol/LKNO_3诱导6h,叶片酶活力达高峰。光照对竹子酶活力具诱导作用,其中以光强5000lx 诱导12h、8000lx 诱导8h 最佳。中龄叶对 KNO_3和光照的诱导反应最敏感,诱导产生的酶活力比嫩叶和老叶高,尚未展开的嫩叶酶活力极低。不同叶龄叶酶活力,与叶片氨基氮和叶绿素含量呈极显著的正相关。在竹根中未能检测出硝酸还原酶活力。  相似文献   

Effect of Homobrassinolide Application on Metabolic Activity and Grain Yield of Wheat under Normal and Water-Stress Condition     

R. K. Sairam 《Journal of Agronomy and Crop Science》1994,173(1):11-16

Activities of nitrate reductase, glutamine synthetase along with rate of photosynthesis, chlorophyll content, soluble protein, relative water content, transpiration and diffusion resistance were estimated in wheat cv. C306 at anthesis stage under irrigated and moisture-stress condition and two levels (0.1 and 1.0 ppm) of homobrassinolide application. Yield and yield attributing parameters were recorded at harvest. Moisturestress adversely affected relative water content, transpiration, net photosynthesis, nitrate reductase and glutamine synthetase activity, chlorophyll and soluble protein content.
Homobrassinolide application increased leaf relative water content and transpiration and decreased diffusion resistance in water-stressed and recovered plants. Homobrassinolide application also had positive effect on nitrate reductase and glutamine synthetase activities, photosynthesis, chlorophyll and total soluble protein content in stressed, irrigated and revived plants. The beneficial effect was also observed on grain yield and yield attributing parameters such as grain number per ear, 1000 grain weight, ear number per plant and harvest index. It was concluded that homobrassinolide induced promotion in metabolic activity was mediated through increased enzyme protein synthesis as well as uptake of water resulting in enhanced relative water content under moisture-stress.  相似文献   

棉花合理施用氮肥及其生理指标的研究初报   总被引：10，自引：0，他引：10  

许德威  陈新德 《中国农业科学》1991,24(1):42-46

 硝酸还原酶（Nitrate reductase，简称NR，下同）是氮素同化的关键酶，与植物对无机氮的利用有着密切的关系。我们在棉株不同生育期分析了棉株顶端下第四片主茎叶的NR活力。结果表明，在蕾期NR活力最高，花铃期急剧下降。NR活力与棉株生长呈正相关。增施氮肥可以提高NR活力。在合理施用氮肥下，蕾期棉株叶片的NR活力在0.2-0.4μmol NO#-2/30分·克鲜重范围，棉株生长正常，而氮肥施用不当，叶片酶活力过高或过低，则导致棉株徒长或早衰。NR活力可作为棉花氮素营养诊断的生理指标。  相似文献   

Yield and Agronomic Traits of Norin 10-derived Spring Wheats Adapted to Northwestern Mexico     

R. L. Villareal    S. Rajaram  E. Del Toro   《Journal of Agronomy and Crop Science》1992,168(5):289-297

Activities of enzymes nitrate reductase (NR) and nitrite reductase (NiR) were determined in rice seedlings differing in salt tolerance raised under increasing levels of NaCl salinity. Salinity caused marked increase in in vivo NR activity in roots and shoots of salt tolerant cvs. CSR-1 and CSR-3 whereas in salt sensitive cvs. Ratna and Jaya a marked inhibition in in vivo NR activity was observed under salimzation. Under both controls as well as salt treatments in all cultivars roots always maintained higher level of in vivo NR activity than shoots. In vitro NR activity increased in both roots and shoots of all cultivars during early days of growth with maximum at 10–15 days and decreased thereafter. In salt tolerant cultivars salinity caused an increase in in vitro NR activity in shoots but not in roots whereas in salt sensitives activity of the enzyme was always more in salt stressed seedlings compared to controls. Salinity increased NiR activity in seedlings of sensitive cultivars whereas in tolerants suppression in root NiR activity was observed due to salinity. Like NR the activity of NiR was also higher in roots than shoots. 1 M NaCl in the enzyme assay medium suppressed in vivo NR activity in roots of 15 days old nonsalinized seedlings with more suppression in sensitive cultivars than tolerants. Results suggest possible different behaviours of nitrogen assimilatory enzymes in rice cultivars differing in salt tolerance and that salt tolerance ability is associated with high in vivo NR activity in seedlings and its further activation under salinization.  相似文献   

猪肝脏HMG-CoA还原酶基因的部分序列克隆及分析     

胡彩虹  夏枚生  江霞  孙海香 《中国畜牧杂志》2005,41(2):16-18

从杜长嘉猪的肝脏中提取基因组RNA ,进行反转录 聚合酶链式反应 (RT PCR) ,获得 1条近 175bp的片段 ,克隆于pUCm TVector后进行序列分析结果表明 ,该片段为HMG CoA还原酶催化域cDNA的部分序列 ,与GenBank登录的序列基本一致  相似文献   

Nitric oxide-overproducing transformants of Pseudomonas fluorescens with enhanced biocontrol of tomato bacterial wilt     

Yanqing Wang  Qian Yang  Yukio Tosa  Hitoshi Nakayashiki  Shigeyuki Mayama 《Journal of General Plant Pathology》2005,71(1):33-38

The relation between nitric oxide (NO) production and the protective ability of Pseudomonas fluorescens T5 against bacterial wilt disease in tomato was examined. The endogenous nitric oxide reductase gene of T5 was disrupted by homologous recombination using a suicide plasmid. Three disruptants were obtained, and all had higher levels of NO production. Infection with Ralstonia solanacearum was reduced in tomato plants treated with the NO-overproducing transformants compared with the wild type. These results suggest that the modification of pseudomonads to increase their level of NO production is a new approach to enhancing their biocontrol efficacy.  相似文献