用二次正交旋转组合设计优化马占相思增殖培养基   总被引：5，自引：0，他引：5  

徐位力  苏开君  王伟平  王光  李杰 《广东林业科技》2003,19(4):13-16

在马占相思组织培养初步取得成功的基础上，采用二次正交旋转组合设计对马占相思增殖培养基进行优化，建立增殖率(Y)对Ca^2 浓度(X1)、6-BA浓度(X2)及NAA浓度(X3)3个试验因子的正交回归模型：Y=2．280-0．168X1-0．259X2 0．185X1^2-0．210X2^2 0．167X3^2 0．326X1X2。从模型推知，当Ca^2 浓度为0．58倍常规MS培养基浓度(255g／L)，6BA为0．76mg／L，NAA为0．16mg／L时，增殖率达最大值为4．32，实验结果与预测值相符。  相似文献   

粉枝莓的组织培养与植株再生   总被引：1，自引：0，他引：1  

罗建 《林业科技》2003,28(1):46-48

利用粉枝莓的茎段和幼嫩叶片作为外植体，研究了不同激素水平对诱导产生愈伤组织、芽和根及完成植株再生的影响。试验结果表明：MS 6-BA0.5 NAA0.2培养基对诱导愈伤组织和芽的分化效果最佳，1/2MS IBA0.3对促进生根效果较好，在两步移栽法中，移栽存活率分别为94％和98％。  相似文献   

生态公益型国有林场生态公益林现状及培育策略的探讨     

冯随起 《福建林业科技》2002,29(Z1):43-46

分析了南平葫芦山国有林场生态公益林建设现状、存在问题,从政策保障、资金支持、培育措施、发展个体经济、提供资金补偿等方面,对生态公益林培育策略进行探讨。  相似文献   

番木瓜组织培养概况   总被引：9，自引：0，他引：9  

吕平  韦丽君  苏文潘  叶启腾 《热带农业科技》2006,29(2):31-33

概述了近年来国内外番木瓜组织培养技术研究进展,并对番木瓜组织培养中影响因素、存在问题及今后发展进行了探讨。  相似文献   

草菇床栽料面保湿对菌丝生长和生物效率的影响   总被引：1，自引：0，他引：1  

纪开萍  何明霞  王文兵 《热带农业科技》2006,29(4):10-12

在西双版纳6～7月,对草菇菇房空间及菇床料面的喷雾温水保湿试验结果:喷30～45℃温水可保持料面湿润,促进培菌期草菇菌丝生长,促进出菇,不影响幼菇蕾生长,喷温水后菇产量为对照(菇床料面不喷温水)的280%～332%。  相似文献   

驱蚊香草的组织培养技术     

李际红  周庆和  谢会成 《中南林业科技大学学报(自然科学版)》2005,25(5):92-96

利用驱蚊香草茎段进行组织培养,研究了添加在培养基中不同激素组成、浓度、培养条件等因素对不定芽诱导、继代增殖、防止玻璃化以及生根的影响.结果表明:适于驱蚊香草不定芽诱导的培养基为MS 6-BA1.5 mg/L NAA0.05 mg/L,适于继代增值的培养基为MS 6-BA1.5 mg/L NAA0.05mg/L,适于生根的培养基为配方为1/2MS IBA0.5mg/L NAA0.1 mg/L;用透气设施的封口材料明显地降低驱蚊香草的玻璃化;基质培养基生根优于琼脂培养基.  相似文献   

Effects site conditions on industrial fiber plantations of Lar/x olgensis     

WANG Shu-li WEN Jing YANG Zhen MA Feng-ming 《林业研究》2006,17(3):255-258

Plot sampling was conducted in the second cultivation areas ofLarix olgensis in Heilongjiang Province, China. By analyzing plot investigation data, wood properties, and kraft pulps of 840 plots and 248 sample trees in industrial plantations of L. olgensis on different sites, we examined the growth process of L. olgensis industrial plantation with suitable structure, the wood fiber feature, chemical composition, physical performance and pulp characteristics. It is suggested that site conditions have major effects on plantation growth, fiber contains, fiber length, rate between fiber length and fiber width, pulping rate and pulp physics intensity. The best site for L. olgensis industrial plantation growth is site class Ⅰ and site class Ⅱ, which are on lower locations. Site condition has an obvious influence on the wood characteristics. Within the range of site conditions and stand densities studied, the worse the site condition, the less the fiber contains, the shorter the fiber length, and the more the 1% NaOH extraction. This kind of relationship becomes more obvious as stand age increases. However, the influence of site condition on pulping rate and pulp physics intensity is not obvious. The result provides theoretical base for cultivation of industrial fiber plantation of L. olgensis.  相似文献   

果树组织培养中褐化现象的研究进展   总被引：9，自引：0，他引：9  

庞勇 《甘肃林业科技》2004,29(1):16-18

介绍了果树植物组织培养褐变的发生机理，发生褐变的主要果树种类，综述了近年来果树植物组织培养中参与褐变现象的酚类物质、酚氧化酶以及多种影响因素的研究进展。  相似文献   

Comparative study on antioxidative system in normal and vitrified shoots of Populus suaveolens in tissue culture     

Lin Shanzhi  Zhang Zhiyi  Lin Yuanzhen  Liu Wenfeng  Guo Huan  Zhang Wei  Zhang Chong 《中国林学(英文版)》2004,6(3):1-8

To explore the physiological and biochemical mechanism of the occurrence of vitrified shoots of Populus suaveolens in tissue culture, the changes in water, chlorphyll, lignin, H₂O₂, phenylalanine ammonialyase (PAL), malonaldehyde (MDA), protective enzymatic systems, and some key enzymes involved in the ascorbate- glutathione cycle were comparatively studied in both normal and vitrified shoots of P. suaveolens. The results show that the lower activities of peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), glutathione reductase (GR) and PAL, and the less contents of chlorphyll, lignin, ascorbate (ASA) and reduced glutathione (GSH) as well as the lower ratios of ASA / DHA and GSH / GSSG are observed in vitrified shoots than in normal ones during the whole culture period. While in comparison with normal shoots, the higher activity of superoxide dismutase (SOD) and the more concentrations of water, H₂O₂, MDA, dehydroascorbate (DHA) and oxidized glutathione (GSSG) are found in vitrified shoots. Statistical analysis indicates that the enhanced activity of SOD and the decreased activities of CAT and POD as well as some enzymes involved in the ascorbate-glutathione cycle might be closely correlated to the accumulation of H₂O₂. The less regeneration of ASA and GSH and the lower capacity of the ascorbate-glutathione cycle observed in vitrified shoots might be due to a significant decrease in APX, MDAR, DHAR and GR activities and a decline in redox status of ASA and GSH. The decreases in chlorphyll content might result in a decline in photosynthesis. The lower activities of POD and PAL could result in the decrease of lignin synthesis and cell wall ligination, which might be the key factor leading to the increase in water content. It is concluded that the deficiency of detoxification capacity caused by the lower capacity of the ascorbate-glutathione pathway and the decreased activity of protective enzymatic system might lead to the large accumulation of H₂O₂ and the enhancement of membrane lipid peroxidation, which might be the main cause leading to the occurrence of vitrifying shoots of P. suaveolens in tissue culture. [Supported by National Natural Science Foundation of China (Grant No. 30271093) and the Foundation of State-designated Base for Biology Researching and Teaching in Beijing Forestry University]  相似文献   

红叶石楠组培苗瓶外生根技术研究     

吴丽君  江淑萍  卓年福  郑丽萍  黄建华  李朝晖  沈继南 《福建林业科技》2007,34(4):4-7,12

以苗龄为40 d的红叶石楠组培继代芽苗为瓶外生根试验材料,采用多因素正交试验设计研究不同栽培基质、不同激素、不同激素浓度处理及处理时间对红叶石楠继代芽苗瓶外生根诱导的影响,继代芽苗扦插前的不同炼苗方式及扦插后施肥方式对芽苗瓶外生根率的影响。结果表明:A3B1C3D2组合(珍珠岩、生根粉ABT 200 mg.L-1浸泡处理30 min)获得59%瓶外生根率,而A3B1C1D2(珍珠岩、ABT 50 mg.L-1浸泡处理30 min)为最理想的组合;芽苗在自然散射光下炼苗15~20 d,生根率可提高到81%;栽后适时追施营养液有利于芽苗的高生长。继代芽苗瓶外生根技术的应用减少组培苗培养工序,促进红叶石楠的组培快繁效率,降低组培苗的生产成本。  相似文献