An investigation on the gamete quality of Black Sea trout (Salmo trutta labrax) broodstock fed with mealworm (Tenebrio molitor)     

&#x;rem Gelinek  Güne Yamaner 《Aquaculture Research》2020,51(6):2379-2388

In this study, the effect of combined feeding Black Sea trout (Salmo trutta labrax) broodstock with commercial feed and mealworm larvae (Tenebrio molitor) for 50 days before the breeding period in order to evaluate the gamete quality. While the control group was fed with commercial feed, mealworm larvae were given as an additional protein source 2 days a week and 3 days a week to the experimental groups formed from female and male individuals. In addition to the growth parameters, the number of eggs (number/individual) and the egg diameter (mm), sperm volume (ml), density of spermatozoa (×10⁹ cell/ml), total motility (%), progressive motility (%) and average curvilinear velocity (VCL, µm/sec) values were determined. At the end of the study, the highest live weight gain was found as 46.2 g in the control group of male. The egg diameters were 4.3 ± 1.8 mm and 4.5 ± 1.4 mm in the worm treatment groups fed twice and three times with mealworm respectively. As a result, it was determined that feeding fish with mealworm larvae as an addition to the commercial diet in female individuals did not affect the amount of eggs (p > .05), but the egg diameters were significantly smaller in the control group than the mealworm groups (p < .05). In spermatological characteristics, only the amount of sperm was different between the groups (p < .05); all other parameters were found to be similar to each other with no statistical differences (p > .05).  相似文献   

利用DAD1反义片段转化创建菜薹可调控雄性不育材料   总被引：1，自引：0，他引：1  

陈国菊  徐飞  雷建军  曹必好 《园艺学报》2009,36(5):685-692

 菜薹因为没有好的雄性不育材料或自交不亲和系,至今尚无一代杂种用于生产,根据拟南芥及白菜型油菜的花药不开裂基因DAD1的保守序列设计引物,扩增菜薹的DAD1基因片段（DAD1F）,构建反义DAD1F植物表达载体,用农杆菌介导法转化菜薹,对转基因植株进行分子检测,鉴定其雄性不育性并进行育性恢复试验.克隆得到的菜薹的DAD1基因片段大小为678 bp,命名为BrcpDAD1F,其序列与拟南芥和白菜型油菜的DAD1高度同源,同源率分别为88％和99％;共得到了12株转基因植株,有6株在转录水平上得到表达,表现为雄性不育,花器官畸形,花粉活力低,萌发率不到10％,且开花后不能结角果或结空角果,或者得到极少种子但种子不萌发;用对照的花粉给转基因植株授粉可使其正常结实.以500 μmol·L^-1茉莉酸甲酯处理可使其雄性不育得到恢复,花粉可以在柱头和培养基上萌发,具有受精能力。T₁代可育株与不育株的比例都呈1:3分离, T₂代不同株系的育性分离比例不同,有些株系继续呈1:3的分离,有些株系全是可育株或全是不育株,说明反义抑制呈单基因稳定遗传。  相似文献   

洋葱细胞质雄性不育系选育研究进展   总被引：7，自引：1，他引：6  

吴海涛  马蓉丽  刘洪炯  焦彦生 《园艺学报》2009,36(2):297-302

 介绍了国内外洋葱细胞质雄性不育（CMS）系的选育及利用概况;对洋葱细胞质雄性不育机理和相关分子标记进行了综述;在此基础上对洋葱雄性不育的研究提出了几点建议。  相似文献   

甜菜单胚雄性不育系及保持系雌蕊形态学及细胞学研究     

吴则东  ;王华忠  ;韩英  ;王丛玲  ;肖春山 《中国甜菜》2009,(3):13-15

以甜菜单胚雄不育系和保持系为材料,用光学显微镜对其雌性器官各部发育进行了比较解剖学观察．结果如下：甜菜雌蕊由3—5枚心皮合生而成,外形可分为柱头、花柱和子房三部分。各部解剖学特征：（1）柱头3—5枚离生稍向外生长,柱头正面由大量乳突细胞组成;（2）花柱短小,半开放形,开口较小,其内充满腺毛状结构;（3）半下住子房,心皮合生一室,在外侧愈合交界处,有发达的盘状蜜腺;胚珠1枚基底胎座,曲生形胚珠,双珠被厚珠心．蓼形胚囊,并有盲囊结构产生。研究表明,甜菜雄不育和可育的雌蕊、胚珠、胚囊形态结构相似,均可正常发育,两者均具有受精与结实能力．  相似文献   

散生指天小辣椒“满天星”的选育     

陈炳金  杨朝进  唐成书 《辣椒杂志》2009,(2):13-15

满天星是以辣椒胞质雄性不育系E4A为母本,恢复系E28C为父本配制的中熟辣椒一代杂种。果实羊角形,纵径6～8cm,横径1．0-1．2cm,平均单果质量4．5g。青熟果绿色,老熟果鲜红色,辣味香浓。每667m。产鲜红椒1800～2400kg,干椒300～400kg。抗CMV和疫病,中抗TMV,适宜全国各地早春露地和保护地栽培。  相似文献   

Life-history variation in relation to nest site abundance in males of the freshwater goby Tridentiger brevispinis     

Daisuke Takahashi 《Ecology of Freshwater Fish》2008,17(1):71-77

Abstract –  Age at maturity in males of the freshwater goby Tridentiger brevispinis , a species with exclusive paternal care, was investigated in two populations in Lake Biwa, Japan, that differed markedly in nest site abundance. At Ohmi-maiko, where nest sites were scarce, most males matured at age 3, and mean body size of males guarding eggs in nests was larger than that of males sampled randomly (including both guarding and nonguarding males) in the population. Conversely, at Minamihama, where nest sites were abundant, many males matured at age 1, and there was no difference in body size between guarding males and males collected randomly. The slope of regression lines between body size of the guarding male and the number of eggs in his nest was greater in Ohmi-maiko than in Minamihama. These results suggest that the shortage of nest sites enhances reproductive success in larger males, probably through male–male competition for nest sites and female mate choice for larger males. I conclude that in nest spawners, the availability of nest sites should strongly affect life-history traits of males through sexual selection.  相似文献   

栽培稻种间近等基因系杂种育性研究   总被引：1，自引：2，他引：1  

陶大云  徐鹏  胡凤益  杨友琼  李静  周家武  Monty P.JONES 《中国水稻科学》2002,16(2):106-110

 为了研究亚洲栽培稻与非洲栽培稻种间杂种不育的遗传基础，以亚洲栽培稻品种WAB56-104为测验种与来自种间回交组合WAB56-104/CG14//WAB56-104///WAB56-104的14个种间近等基因系测交，对亲本及杂种的花粉及小穗育性研究表明，这14个近等基因系带有来自非洲栽培稻品种CG14的3个互不等位的配子消除位点和2个互不等位的花粉不育基因位点, 其中至少有2个配子消除位点未曾报道过。  相似文献   

爪哇稻Wanilava细胞质雄性不育系的创建及AFLP指纹图谱分析   总被引：6，自引：0，他引：6  

陈永军  谢崇华  张景荣  郑文静  胡尚连  魏炘  王琦琳  杞从婷  刘永胜 《中国水稻科学》2008,22(1):28-32

初步鉴定了爪哇稻Wanilava细胞质雄性不育系的特征特性,并以2套水稻（Oryza sativa）同核异质材料273JW（爪哇型胞质不育系）、273D（D型胞质不育系）及其对应的保持系273B, 803JW（爪哇型胞质不育系）、803WA（野败型胞质不育系）及其对应的保持系803B为研究对象,利用AFLP 分子标记技术构建了JW型不育胞质的指纹图谱,分离了JW型胞质与野败型胞质及保持系的差异片段。  相似文献   

水稻雌雄不育突变体Osfma2的细胞学观察及基因图位克隆     

张涛荟  王海宇  万华  张莉萍  谢振威  陈可毅  何晓栋  赵志刚  万建民 《中国水稻科学》2022,36(1):13-26

【目的】通过构建分离群体定位并克隆水稻雌雄不育基因OsFMA2,并对其在水稻育性调控中的功能进行探究。【方法】通过EMS诱变水稻宁粳4号得到稳定的不育突变体,对突变体进行表型及细胞学观察,利用精细定位和Mut-map相结合的方法克隆了水稻雌雄不育基因OsFMA2,通过实时荧光定量PCR检测其在各组织表达水平差异,利用水稻原生质体表达系统进行OsFMA2蛋白的亚细胞定位。【结果】细胞学观察发现突变体为雌雄不育。利用极端个体将其精细定位于水稻第6染色体长臂448 kb的区间内。结合全基因组重测序结果,最终确定OsFMA2为目标基因。该基因编码一个复制蛋白,在单子叶植物中高度保守。OsFMA2具有组织特异性,在幼穗中高表达。亚细胞定位结果显示OsFMA2蛋白定位于细胞核中。【结论】OsFMA2在幼穗中高表达,可能参与了水稻雄配子第一次减数分裂前期同源重组过程,同时,OsFMA2的表达对雌配子发育也产生了不利影响。  相似文献   

烯效唑干粉拌种降低小麦不育系株高的效应   总被引：1，自引：0，他引：1  

黄康龙  赵颖  莫俊杰  梁杰恩  黄华彬  刘丽霞  周鸿凯  何觉民 《麦类作物学报》2010,30(4):634-637

为了加大杂交小麦制种时的父母本株高差异,获得良好的制种效果,用不同剂量的烯效唑干粉和待播不育系种子均匀混合,以探索烯效唑干粉拌种的适宜剂量和不同不育系对烯效唑的敏感性.结果表明,烯效哇干粉拌种可以显著降低小麦不育系的株高,30 mg·kg-1是多数供试不育系的适宜拌种剂量;不同不育系对烯效唑的敏感性有一定差异,在烯效唑用量为20 mg·kg-1时,各不育系的株高降低率差异不显著;在烯效唑用量为30 mg·kg-1和40 mg·kg-1时,各不育系的株高降低率以268S为最高,极显著高于海大S-1和海大S-2,而与2110-2-5-2-5S无显著差异.  相似文献