Mapping Major Restore Genes for C-type Cytoplasmic Male Sterility in Maize with SSR Marker     

TANG Ji-hua  LIU Zong-Hua  CHEN Wei-cheng  HU Yan-min  JI Hong-qiang  JI Liang-yue  XIE Hui-ling  HUANG Xi-lin 《中国农业科学(英文版)》2002,1(3):269-273

Through observation about the restoration of male fertility of F2 and BC1 progeny, we found that the restoring line Fengke1 had two duplicating restorer genes. The restorer gene R f5 in Fengke1 background was located on chromosome 5L by SSR method; it linked with bnlg1711, bnlg1346 and phi058,the genetic distances with bnlg1711, bnlg1346, and phi058 were 7.51cM, 1.68cM, and 9.87cM respectively;the restorer gene R f4 was mapped on chromosome 8S linked with bnlg2307.  相似文献   

Metabolism of Reactive Oxygen Species in the Cytoplasmic Male-Sterile Cotton Anther   总被引：2，自引：0，他引：2  

JIANG Pei-dong ZHU Yun-guo WANG Xiao-ling ZHU Wei ZHANG Xiao-quan XIE Hai-yan WANG Xue-de 《中国农业科学(英文版)》2007,6(3):275-280

Reactive oxygen species （ROS） in plant cell, including superoxide （O2^-）, hydrogen peroxide （H2O2）, and malondialdehyde （MDA）, are thought to be important inducible factors of cell apoptosis if excessively accumulated in cells. To elucidate the metabolic mechanism of ROS production and scavenging in anthers of the cytoplasmic male-sterile （CMS） cotton, CMS line, maintainer, and hybrid F1 anthers, were employed for studying the relationship between CMS and metabolism of ROS, by comparing ROS changes in the sterile and fertile anthers at different developmental stages. The results showed that during the abortion preliminary stage （sporogenous cell division stage）, anthers of CMS line had higher contents of O2^-, H2O2, and MDA than those of maintainer or hybrid F1. Simultaneously, the higher activities of superoxide dismutase （SOD）, catalase （CAT）, and peroxidase （POD） in scavenging ROS were measured in the anthers of the CMS line, indicating that an increase of ROS in anthers of abortion preliminary stage had an inducible effect on the antioxidant enzymes. But during the abortion peak of CMS anther （pollen mother cell meiosis stage）, on the one hand, contents of O2^-, H2O2, and MDA were extraordinarily high in CMS anthers, on the other hand, the activities of SOD, CAT, and POD were excessively low, which disrupted the balance between the production and elimination of ROS and led to pollen mother cells apoptosis at this stage. In the following two stages （uninucleate microspore stage and mature pollen stage）, the contents of O2^- and H2O2 in the aborted anthers were approximated to contents in the fertile anthers of the maintainer and hybrid F1. However, MDA contents were continuously raised and enzymic activities of SOD, CAT, and POD were consistently decreased in sterile anthers, which indicated that ROS still had harmful effects on the anthers after the apoptosis of the male cells. Excessive accumulation of O2^-, H2O2, and MDA and significant reduction of ROS scavengingenzyme activities were coinstantaneous with male cells apoptosis in the anthers of the cotton CMS line. But when the restorer gene was transferred into the CMS line, excessive production of ROS could be eliminated in the anthers of hybrid F1.  相似文献   

萝卜胞质不结球白菜雄性不育系叶片黄化现象的研究   总被引：2，自引：1，他引：2  

任成伟  曹寿椿 《南京农业大学学报》1990,13(3):37-41

用2个 ogura 萝卜细胞质不结球白菜雄性不育系的衍生株系为母本,99个不结球白菜品种为父本,配组198个测交 F_1代。秋冬栽培期安排田间随机区组试验。试验发现了在 ogura 细胞质背景下不结球白菜叶片的黄化规律,其黄化程度与细胞核基因型、植株生长时期和环境温度有密切关系。方差分析表明,测交 F_1代间、父本间和母本间的平均黄化指数存在极显著差异。试验虽未筛选到叶片完全不黄化的测交组合,但发现有一些组合的黄化很轻微,有进一步研究的价值。试验还发现了几个对 Ogura 胞质黄化敏感性弱的父本好材料,其中绝大多数为青梗品种。文章讨论了进一步改良这类不育材料黄化现象的可行途径。  相似文献   

冈型及D型杂交稻的选育、利用和遗传研究   总被引：1，自引：0，他引：1  

李实蕡 《杂交水稻》1997,(Z1)

冈型及Ｄ型杂交稻是冈型及Ｄ型各同质不育系组配成的一群杂交稻。冈型及Ｄ型不育系是通过籼亚种内品种间杂交途径，育成有生产价值的雄性不育系的首例，是继野败型、ＢＴ型杂交稻之后大面积投产的非野败胞质的新型不育系。它为培育水稻雄性不育系，开发新胞质源展示了新的可行途径，开始打破杂交水稻生产上单一野败胞质的局面，经济、社会、生态效益均十分显著。在组配中，早熟杂交稻的恢复系和改良不育系异交习性的保持东选育上也获较好效果。比较系统观察了细胞质效应在不育系主要性状、小孢子败育进程、过氧化物酶同功酶谱、可恢复性、组配杂种Ｆ１性状以及杂种后代育性表达分离等的影响，表明水稻品种细胞质源的遗传多样性，并对不育系及其组配杂种Ｆ１性状形成有特定遗传效应。在杂种组配规律研究上，观察到杂种性状观察值与父母本的一般配合力和特殊配合力相对效应值之和（即总效应值），呈显著线性关系，另根据其父母本的一般配合力相对效应值之和，粗放预测杂种性状优势的可信度，也是高的，对单株产量等性状实际观察值的检验表明．杂种亲本即不育系、恢复系须选择一般配合力高或中者，才有可能获较强优势杂种。关于水稻育性遗传也进行了研究和探讨。  相似文献   

粳稻光(温)敏核质互作不育系(SA)的育性和可恢复性研究   总被引：4，自引：0，他引：4  

杜士云  王守海  李成荃  王德正  罗彦长  吴爽 《杂交水稻》2006,21(4):69-73,77

连续3a的分期播种试验表明，聚合光（温）敏核不育系S和核质互作不育系A的不育基因转育而成的粳稻光（温）敏核质互作不育系SA的育性对光温反应迟钝，在长日高温、适温、低温和短日适温、低温下其育性都稳定不育；SA花粉败育类型表现为长日下以典败为主，短日下典染共存，且随着日长的变短和温度的降低，染败花粉有增加的趋势。可恢复性试验表明，不同的恢复系对SA的恢复能力强弱不同，SA的可恢复性一般介于亲本S和A的可恢复性之间。  相似文献   

Factors Influencing the Outcrossed Seed-Setting Obstacles of Photo-thermo Sensitive Genie Male Sterile Rice     

CHEN Xiong-hui  WAN Bang-hui  PENG Hai-feng  LU Yan-peng  LIANG Ke-qin  ZHAO Jing 《水稻科学》2004,(3)

Using photo-thermo sensitive genie male rice (PTGMS) Pei' ai 64S, W7415S, W6154S, N26S, Annong S, Nongken 58S, 7001S and 5088S as female parents and conventional indica lines 8258 and U89 as male parents, the factors affecting outcrossed seed-setting were analyzed. The PTGMS had obstacles in outcrossed seed setting influenced by inheritance and environment at varying degrees. Environmental temperature was regarded as the main factor that resulted in the outcrossed seed-setting obstacles. The sensitive stage was at the early stage of grain filling for outcrossed seed setting. There existed remarkable differences at the sensitivity stage, the duration of sensitive period, the sensitive level and the effective level of outcrossed seed-setting obstacles caused by environmental temperature among different PTGMS lines. Therefore, attention should be paid to outcrossed seed-setting obstacles in selection and utilization of PTGMS lines.  相似文献   

小麦BNS雄性不育基因连锁群分析和主效QTLs初步定位     

杨靖  卫笑  付庆云  曹银萍  茹振钢  李友勇 《麦类作物学报》2022,(11):1334-1342

为初步定位小麦温敏雄性不育系BNS中与不育相关的QTL及其所属连锁群,用BNS及其完全保持系郑麦366的F₂为作图群体,建立自交结实率和花粉可育率两个表型BSA池,选用均匀分布在小麦染色体上的710个SSR分子标记,在BSA池中筛选连锁标记,并进行QTL定位,同时用连锁标记引物在BNS DNA中重扩增,扩增产物序列在小麦基因组中进行比对,验证标记所属染色体并分子定位。结果表明,在2对BSA池中共筛选到12个连锁标记,涉及8个连锁群。单标记分析发现,5个连锁标记（Xwmc396、Xwmc517、Xbarc55、Xwmc332和Xwmc752）与不育QTL紧密连锁。用区间分析法分析发现,有2个主效不育QTL,分别为2B染色体上的 qBS1 （紧密连锁Xwmc332和Xbarc55）和7B染色体上的 qBS2 （紧密连锁Xwmc396和Xwmc517）。两个主效QTL的LOD值均大于5,贡献率均大于13%,且显性效应均大于加性效应。  相似文献   

Frequency of 2n pollen in diploid hybrids betweenSolanum phureja Juz. & Buk. andSolanum chacoense Bitt.     

F. Bani-Aameur  F. I. Lauer  R. E. Veilleux 《Potato Research》1992,35(2):161-172

Summary Solanum phureja (PP)xSolanum chacoense (CC) hybrid families (PC) were evaluated for pollen fertility and frequency of 2n pollen in two seasons in Morocco. PP clones produced high frequencies of 2n pollen, whereas CC clones produced none. The percentage of plants with 2n pollen ranged from 2 to 63% among 20 PC families and was consistent in some PC families over two growing seasons, whereas others varied between fall and spring growing seasons. The expected ratio of 2n to non-2n pollen-producers within a PC family based on a single recessive gene model [i.e. 1∶0 with CC homozygous dominant (PsPs) or 1∶1 with CC heterozygous (Psps)] was observed in only 5 of 20 progenies. Narrow sense heritability was 0.71, allowing gain from further selection. Only clones producing more than 10% 2n pollen exhibited more stable expressivity across seasons. Scientific Journal Series Article 17811 of the Minnesota Agricultural Experiment Station supported in part by the US Agency for International Development, contract no. 608-0160, IAV Morocco Project.  相似文献   

R-ms “临高油1号”转育及超油化生产研究     

王向东  高根来  宁慧云  甄胜虎  张风琴 《玉米科学》2006,14(5):064-065

近几年来，高油玉米种质利用已经进入实质应用阶段，高油玉米新品种层出不穷，已经通过审认定的高油玉米品种有高油115、春油1号、吉油1号等10多个品种，山西省在2003年有两个高油玉米新品种通过了审定。高油玉米种质利用技术也将随着高油玉米研究的深入，从单交种技术逐步发展为以不育行普通杂交种授以高油玉米花粉的遗传三利用技术。临高油1号的选育、不育型临高油1号转育及超油化生产技术研究，使山西省高油玉米种质利用研究达到一个更高的水平，高油玉米商品粮的含油量超过10％的育种目标成为可能。该技术在今后农业产业化调整过程中，将发挥巨大作用。  相似文献   

芥菜型油菜细胞质雄性不育系 6 - 102A的细胞学观察   总被引：7，自引：2，他引：7  

万正杰  王显军  傅廷栋  涂金星 《中国油料作物学报》2006,28(3):268-271

对芥菜型油菜细胞质雄性不育系6 - 102A的花药发育过程进行了显微观察,并与6 - 102B (保持系)和8 - 602A ( tour CMS)进行比较,结果表明:不育系6 - 102A败育彻底,败育可能发生在雄蕊原基分化时期,其主要特 点是雄蕊原基偏离正常的分化轨道,形成花瓣原基,不形成花药,着生雄蕊的位置长出小花瓣,败育时期和方式与 现有报道的油菜细胞质雄性不育不同。保持系6 - 102B的花药发育正常; 8 - 602A败育发生于孢原细胞时期至四 分体时期,主要是孢原细胞时期。  相似文献