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排序方式: 共有109条查询结果,搜索用时 15 毫秒
21.
H. Angélica Aponte R. Patricia Penilla Felipe Dzul-Manzanilla Azael Che-Mendoza Alma D. López Francisco Solis Pablo Manrique-Saide Hilary Ranson Audrey Lenhart Philip J. McCall Américo D. Rodríguez 《Pesticide biochemistry and physiology》2013
Dengue is one of the most important vector-borne diseases worldwide and is a public health problem in Mexico. Most programs in dengue endemic countries rely on insecticides for Aedes control. In Mexico, pyrethroid insecticides (mainly permethrin and deltamethrin) have been extensively used over a decade as adulticides and represented a strong selection for insecticide resistance for dengue vectors in several parts of the country. We studied the type, frequency and distribution of insecticide resistance mechanisms in Aedes aegypti from six municipalities in the state of Guerrero selected on the basis of historically intense chemical control and a high risk for dengue transmission. Ae. aegypti eggs were collected from October 2009 to January 2010 using ovitraps. F1 adults, emerged from these collections, were exposed to permethrin, deltamethrin and DDT in WHO diagnostic tests and showed high resistance levels to both pyrethroids and DDT. This was consistent with the presence of increased metabolic enzyme activities and target site insensitivity due to kdr mutations. Biochemical assays showed elevated esterase and glutathione S-transferase activities in the six municipalities. The V1016I kdr mutation on the IIS6 domain of the sodium channel gene was present in an overall frequency of 0.80. A second mutation, F1534C on the IIIS6 domain of the same gene was also detected, being the first report of this mutation in Guerrero. The multiple resistance mechanisms present in Ae. aegypti from Guerrero state represent a warning for the efficacy of the pyrethroid usage and consequently for the success of the dengue control program. 相似文献
22.
Ranil Waliwitiya Christopher J Kennedy Carl A Lowenberger 《Pest management science》2009,65(3):241-248
BACKGROUND: Aedes aegypti L. is the major vector of dengue fever and dengue hemorrhagic fever. In an effort to find effective tools for control programs to reduce mosquito populations, the authors assessed the acute toxicities of 14 monoterpenoids, trans‐anithole and the essential oil of rosemary against different larval stages of Ae. aegypti. The potential for piperonyl butoxide (PBO) to act as a synergist for these compounds to increase larvicidal activity was also examined, and the oviposition response of gravid Ae. aegypti females to substrates containing these compounds was evaluated in behavioral bioassays. RESULTS: Pulegone, thymol, eugenol, trans‐anithole, rosemary oil and citronellal showed high larvicidal activity against all larval stages of Ae. aegypti (LC50 values 10.3–40.8 mg L?1). The addition of PBO significantly increased the larvicidal activity of all test compounds (3–250‐fold). Eugenol, citronellal, thymol, pulegone, rosemary oil and cymene showed oviposition deterrent and/or repellent activities, while the presence of borneol, camphor and β‐pinene increased the number of eggs laid in test containers. CONCLUSIONS: This study quantified the lethal and sublethal effects of several phytochemical compounds against all larval stages of Aedes aegypti, providing information that ultimately may have potential in mosquito control programs through acute toxicity and/or the ability to alter reproductive behaviors. Copyright © 2008 Society of Chemical Industry 相似文献
23.
Patch and skin bioassays were used in laboratory and indoor tests to evaluate the repellency of (E)-cinnamaldehyde, identified in Cinnamomum cassia Blume bark and essential oil, and a cream containing 5% (w/w) cassia oil against Aedes aegypti (L.) females. Results were compared with those of a known C. cassia compound cinnamyl alcohol, N,N-diethyl-m-toluamide (DEET) and two commercial repellents: MeiMei cream containing citronella and geranium oils and Repellan S aerosol containing 19% DEET. In patch bioassay tests with A. aegypti females, (E)-cinnamaldehyde at 0.153 mg cm(-2) and DEET at 0.051 mg cm(-2) provided 93 and 89% protection at 40 min after exposure. In skin bioassay tests, (E)-cinnamaldehyde at 0.051 mg cm(-2) and DEET at 0.025 mg cm(-2) provided 87 and 95% protection at 30 min after application. (E)-Cinnamaldehyde was significantly more effective than cinnamyl alcohol in both bioassays. In indoor tests with four human volunteers, 5% cassia oil cream provided 94, 83 and 61% protection against A. aegypti females exposed for 30, 50 and 70 min after application respectively. Cassia oil cream was a slightly less effective repellent than MeiMei cream. Repellan S aerosol provided 91% repellency at 120 min after application. Products containing cassia oil merit further study as potential repellents for the protection of humans and domestic animals from blood-feeding vectors and the diseases they transmit. 相似文献
24.
25.
Dengue fever is an important mosquito-borne viral disease in Taiwan. Insecticide resistance has been shown to significantly reduce the efficacy of vector control interventions. The detection of insecticide resistance is an important component in mosquito abatement programs. In this study, we used the insecticide-impregnated papers bioassay method to reveal high levels of resistance to permethrin in the LYPR and field strains of Aedes aegypti. We used the standard glass cylinder method to observe the knockdown effect of paralysis within 2 to 4 minutes after exposing mosquitoes to pyrethroid vapors. Biochemical assays showed elevated detoxification enzyme activities. Glutathione S-transferases, monooxygenases and β-esterases were the enzymes predominantly responsible for the permethrin resistance of Ae. aegypti in Taiwan. Molecular screening for common insecticide target-site mutations revealed the presence of V1023G and D1794Y mutations. Pearson’s correlation analysis was used to investigate the correlations between the allelic frequency of kdr mutation associated increase with the LC50 values of permethrin and the KT50 values of pyrethroid vaporizers. These findings will be used to assess resistance levels, estimate resistance potential, and formulate monitoring and resistance management strategies. 相似文献
26.
de Mendonça FA da Silva KF dos Santos KK Ribeiro Júnior KA Sant'Ana AE 《Fitoterapia》2005,76(7-8):629-636
The insecticidal activities of extracts and oils of seventeen medicinal plants of Brazil have been determined using an Aedes aegypti larvicidal bioassay. Oils from Anacardium occidentalis, Copaifera langsdorffii, Carapa guianensis, Cymbopogon winterianus and Ageratum conyzoides showed high activities with LC50 values of 14.5, 41, 57, 98 and 148 microg/l, respectively. The most active ethanolic extract tested was that from the stem of Annona glabra which presented an LC50 value of 27 microg/l. The potential application of cashew nut oil, an industrial by-product with low commercial value, in the control of the vector of dengue and yellow fever, may be proposed. 相似文献
27.
The larvicidal activity against Aedes aegypti larvae of a stem wood hexane extract of Cybistax antisyphilitica was evaluated. Bioassay-guided fractionation of the crude extract, monitored by larvicidal assay, led to the isolation of a natural quinone identified as 2-hydroxy-3-(3-methyl-2-butenyl)-1.4-naphthoquinone (lapachol). This compound was quite potent against A. aegypti larvae (LC50 26.3 microg/ml). 相似文献
28.
Adriana E. Flores Walter Albeldaño-Vázquez Mohammad H. Badii Gustavo Ponce Garcia William G. Brogdon Barry Beaty 《Pesticide biochemistry and physiology》2005,82(1):66-78
A discriminating dose for permethrin for Aedes aegypti from Baja California, Mexico was determined, and was used to select individuals. Mosquitoes were collected from four different municipalities located in the north and south end of the Baja California Peninsula. Individuals were chosen for further study based on their similar response to the insecticide. We exposed 10 groups of 90 Ae. aegypti females to the discriminating dose (172 μg/ml) and after producing 50% mortality, individuals were divided into two categories: killed and survivors. Each of these groups was dissected to separate the head, thorax, and abdomen. Biochemical tests were performed on the head and thorax to determine resistance-related enzyme activities including: α- and β-esterases, glutathione S-transferase, acetyl cholinesterase, insensitive acetyl cholinesterase, and mixed-function oxidases. The results were compared with those for the susceptible New Orleans strain of Ae. aegypti. All the populations studied showed the consistent presence of α-esterases, with elevated levels in permethrin-selected populations. Although β-esterases and GST levels were present in high proportions, they did not reveal a clear pattern in relation to resistance. 相似文献
29.
Choochote W Chaithong U Kamsuk K Jitpakdi A Tippawangkosol P Tuetun B Champakaew D Pitasawat B 《Fitoterapia》2007,78(5):359-364
Essential oils extracted from ten plant species were screened for repellency against Aedes aegypti mosquitoes. Three oils; Zanthoxylum piperitum, Anethum graveolens and Kaempferia galanga, exerted protection against A. aegypti, with median complete-protection times of 1, 0.5 and 0.25 h, respectively. The protection times were increased significantly by incorporating 10% vanillin. The highest potential was established from Z. piperitum oil +10% vanillin (2.5 h, range=1-2.5 h). Mixtures from pairs of the effective oils possessed slight repellency that ranged from 0-0.5 h. None of the oil combinations repelled A. aegypti for longer than their constituent oil alone. With vanillin added, however, each oil mixture provided improved protection, which was approximately equal to oil on its own. GC/MS analysis revealed that the main component of Z. piperitum fruit oil was limonene (37.99%), with minor amounts of sabinene (13.30%) and beta-myrcene (7.17%). Repellent testing of stored samples of Z. piperitum fruit oil against A. aegypti demonstrated that repellent activity of those kept at -20 degrees C or 4 degrees C was present for a period of at least 3 months. Therefore, the essential oil of Z. piperitum fruit may prove useful in the development of mosquito repellents as an effective personal protection measure against mosquito bites. 相似文献
30.