笔花豆炭疽病大田防治试验研究   总被引：1，自引：0，他引：1  

冯淑芬  林坚毅 《热带作物学报》1994,15(2):103-108

格拉姆笔花豆炭疽病在海南危害严重。大田化学防治试验表明,应用20％多菌灵乳剂,有效成分450g/hm~2·次,在作物生长期和花期,连续喷药3次,可获得满意的防治效果,产量增加36％～60.8％。  相似文献   

Sensitivity of Cercospora beticola isolates from Serbia to carbendazim and flutriafol     

《Crop Protection》2014

Cercospora beticola, causal agent of Cercospora leaf spot (CLS) of sugar beet, is primarily controlled by fungicides. Benzimidazole and demethylation inhibiting fungicides, including carbendazim and flutriafol, have been widely used in Serbia. Since these fungicide groups have a site-specific mode of action, there is a high risk for developing resistance in target organisms, which is the most important limiting factor in Cercospora leaf spot chemical control. A rapid identification of flutriafol and carbendazim resistance can help researchers in examining the potential of different fungicide resistance management practices, as well as in selection of fungicides for use in the areas where resistance has occurred. One hundred single-conidia isolates were collected from 70 representative locations of the sugar beet production region in Serbia. Evaluation of the isolates' sensitivity was based on the reduction of mycelial growth on medium amended with 1.25 μg mL⁻¹ flutriafol and 5 μg mL⁻¹ carbendazim. Resistance to flutriafol and carbendazim was detected in 16% and 96% of the tested isolates, respectively. All isolates resistant to flutriafol were resistant to carbendazim as well, which is the first report of a double resistance to fungicides in C. beticola. Detection of the isolates resistant to flutriafol and carbendazim using Cleaved Amplified Polymorphic Sequence (CAPS) markers confirmed the results of the in vitro tests. The efficacy of carbendazim, flutriafol, azoxystrobin, and tetraconazole at commercially recommended doses was evaluated in field trials where sugar beet plants in plots were inoculated with a mixture of isolates either sensitive and/or resistant to flutriafol and carbendazim. Carbendazim and flutriafol efficacy was very low in plots inoculated with isolates resistant to these fungicides. Presented results will contribute to development of a pathogen population sensitivity monitoring strategy that could be used for an effective CLS management in the region.  相似文献   

高效降解多菌灵芽孢杆菌菌株D-A-2的筛选及鉴定     

马晨  王全 《安徽农业科学》2017,45(36):135-136,220

[目的]筛选能高效降解多菌灵的菌株。[方法]对土壤中能高效降解多菌灵菌株D-A-2进行分离筛选及鉴定。[结果]采用管碟法从土壤中初筛得到24株具有活性的菌株,光谱法复筛得到6株降解能力较强的菌株,并对D-A-2菌株进行形态鉴定、生理生化特性鉴定和16S r DNA全序列分析。[结论]D-A-2菌株降解能力最强,初步鉴定为枯草芽孢杆菌。  相似文献   

四种杀菌剂对苜蓿根和根颈腐烂病菌的室内毒力测定   总被引：7，自引：1，他引：7  

李敏权  杨宝生  侯军  张自和 《草地学报》2002,10(4):270-273

采用室内平皿生长速率法,研究四种杀菌剂对苜蓿根和根颈腐烂病的病原、尖孢镰刀菌、锐顶镰刀菌和半裸镰刀菌的毒力测定。结果表明,四种药剂对三种病原菌都有显著的抑制作用。其中多菌灵、甲基硫菌灵、枯腐宁和枯萎绝四种药剂对锐顶镰刀菌的有效中浓度(EC₅₀)分别为84.93、221.00、53.74和7.90μg/mL,对尖孢镰刀菌EC50分别为70.12、125.76、35.75和5.87μg/mL,对半裸镰刀菌分别为49.55、126.61和221μg/mL。以枯萎绝的抑菌作用最为突出,对三种镰刀菌的EC₅₀都在10μg/mL以下,枯腐宁次之,甲基硫菌灵最差。四种药剂的相关系数均在0.96以上,药剂浓度与抑制作用呈正相关。  相似文献   

液相色谱法检测柑橘中多菌灵残留的方法研究   总被引：1，自引：0，他引：1  

高国文  刘育清  朱家香 《农药科学与管理》2006,27(5):10-11,24

研究了用HPLC-紫外检测器检测柑橘中多菌灵残留的方法,通过改变检测多菌灵农药质量行业标准中的流动相配比,使柑橘样品中杂质与多菌灵实现基线分离,通过用甲醇加冰醋酸取代国标检测多菌灵残留的前处理的二氯甲烷,方法的最低检出限0．2mg／kg, 添加回收率为110％,变异系数为5．2％。  相似文献