激光扫描共聚焦显微镜在园艺植物研究中的应用     

王健  杨青  孟庆杰 《安徽农业科学》2016,(3)

综述了激光共聚焦显微镜的原理以及激光扫描共聚焦显微镜技术在园艺植物细胞学、植物荧光方面、发育生物学和遗传学的应用研究进展,并对其在园艺植物研究中的应用前景进行了展望。  相似文献   

Prospects and results for optical systems for site-specific on-the-go control of nitrogen-top-dressing in Germany     

H. J. Heege  S. Reusch  E. Thiessen 《Precision Agriculture》2008,9(3):115-131

Signals for site-specific nitrogen-top-dressing can be obtained by a sensor mounted on a tractor. The plant appearance can serve as a criterion. The question is which plant criteria provide pertinent information and how this can be indicated. Increasing the nitrogen-supply changes leaf colour from yellow-green to blue-green via the chlorophyll-concentration in the leaves and leads to growth of plants. Present sensing systems measure either chlorophyll concentration in the leaves, total area of the leaves or crop resistance against bending. The aim and purpose of this study is to outline prospects for application. Therefore, the emphasis is on results and not on experimental methods, to which references are given. Mainly optical sensing systems relying on reflectance or fluorescence are dealt with. Good signals of the nitrogen-supply can be obtained from the red edge plus the near infrared range of the reflectance. The results with some new spectral indices were better than those with standard spectral indices. Fluorescence sensing instead of reflectance sensing eliminates erroneous signals from bare soil. However, only low supply rates were clearly indicated. The biomass of the crop or the total area of its leaves is a very important criterion. Reflectance indices can take this into account. Fluorescence signals are barely influenced by this parameter.  相似文献   

Identification of recombinant baculovirus and determination of virus titer with fluorescence quantitative PCR assay     

TONG Xia-sheng  MENG Zhe-feng△ 《园艺学报》2007,23(8):1623-1626

AIM: To develop a real-time PCR assay based on TaqMan technology for the identification of recombinant baculovirus and determination of virus physical titers in Bac-to-Bac system.METHODS: The recombinant baculovirus containing human IL-18 gene was produced using Bac-to-Bac system.A 10-fold serially diluted primary viral stock was used for plaque assay and DNA extraction.Bacmid (baculovirus plasmid) was 10-fold serially diluted and served as standards.Real-time PCR amplification of the IL-18 gene was performed in triplicate for each diluted recombinant virus.At the same time,plaque assays were performed using overlay agarose method.RESULTS: The standard linear range (101 to 108 copies) for quantitation was achieved with the standard curve.We also find that the“vg/mL”titer value is generally about 10 times than“pfu/mL”titer of the same recombinant virus stock.CONCLUSION: A TaqMan real-time PCR method is established to identify the recombinant baculovirus and determine the“vg/mL”titer of virus.The method is rapid and quantitative over a wide range of virus titers.  相似文献   

The construction of bicistronic eukaryotic vector carrying green fluorescent protein and hytk gene and its expression in bladder carcinoma     

YE Chuan-zhong  LIN Zhen  CHEN Shi-ping  ZHANG Fang-lin  PEI Xue-tao  LI Liang  FENG Kai 《园艺学报》2001,17(9):825-829

AIM: To facilitate the suicide gene delivery into neoplasm, a chimeric gene of HSV-tk and green fluorescent protein (gfp) was constructed. METHODS: Molecular cloning technique was used to construct this kind of eukaryotic vector. The internal ribosome entry site (IRES) of encephalomyocarditis virus (EMCV), which could coordinate expression of two genes in a single vector, was optioned. By using liposome-mediated transfection, eukaryotic expression vector tgCMV/hytk-IRES-gfp was transfected into human bladder carcinoma cells EJ. RESULTS: A bicistronic eukaryotic vector carrying gfp and hygromycin phosphotransferase-thymidine kinase fusion (hytk) gene was constructed. The results of PCR and microscopy detection show that the hytk-IRES-gfp gene was successfully transferred into EJ cells. There were no differences in the growth pattern or the morphology between EJ and EJ/hytk-GFP cells. In vitro experiments demonstrated dose- and time-dependent cell killing by transduction of the hytk-IRES-gfp gene followed by GCV treatment. The IC50 (the concentration required to elicit 50% growth inhibition) was 2.16 mg/L in treatment with GCV for 72 hours. CONCLUSION: These results suggest that this new kind of eukaryotic vector could serves as a new tool and method for neoplasm gene therapy.  相似文献   

Differential Gene Expression and Analysis in Venturia nashicola-inoculated and Uninoculated Leaves of the New Highly Resistant Germplasm 03-04-034     

Haie ZHANG  Lijuan GAO  Longfei LI  Jintao XU  Baofeng HAO  Yang YANG  Yonghong LI 《农业科学与技术》2017,18(3)

  相似文献   

基于叶绿素荧光光谱分析的光能利用效率研究   总被引：1，自引：0，他引：1  

周丽娜  于海业  于连军  张蕾  隋媛媛  任顺 《农业机械学报》2014,45(7):255-259

以水稻为研究对象,利用叶绿素荧光光谱对水稻叶片的光能利用效率进行分析。研究中,采集水稻叶片的叶绿素荧光光谱,同步测定净光合速率和光合有效辐射,并进行光能利用效率的计算。以红光区F685和远红光区F732为光谱特征参数的分析显示,F685、F732及参数组合F685/F732与光能利用效率显著线性相关,因此分别以F685、F732和以F685/F732为模型输入建立了光能利用效率预测模型并进行对比,发现基于F685/F732的预测模型精度更高。由于CO2的同化过程受气孔导度的影响,而叶片温度是气孔导度的信号,且光能利用效率受叶片温度影响显著,为提高模型预测精度,也将叶片温度作为模型输入,建立了基于F685/F732和叶片温度的光能利用效率预测模型,决定系数为0.885。  相似文献   

NaCl胁迫对普陀樟叶绿素荧光参数的影响   总被引：3，自引：0，他引：3  

李影丽  汪奎宏  杜国坚  许利群  杨华  肖纪年 《安徽农业科学》2008,36(22)

[目的]探讨NaCl胁迫对普陀樟叶绿素荧光参数的影响。[方法]以1年生普陀樟叶片为试材,采用田间试验的方法,设置3种处理(NaCl溶液浓度分别为3、5、7 g/L),以清水为对照(CK),研究盐处理对苗木生长量、叶绿素含量及叶绿素荧光参数的影响。[结果]NaCl胁迫下,普陀樟苗高生长量下降,叶片的叶绿素含量下降趋于平稳;PSⅡ潜在活性(Fv/Fo)、原初光能转化率(Fv/Fm)变化一致,整体呈上升趋势;随NaCl胁迫的加剧,Fo变化最明显,叶绿素荧光参数间相关性先增强后减弱。[结论]普陀樟具有较好的抗盐性和广泛适应性。  相似文献   

超级稻P88S/0389光合特性初探     

张吉贞  孟卫东  曹赛  邓晓峰 《安徽农业科学》2008,36(29)

[目的]通过比较超级稻P88S/0389与其亲本P88S和R0389剑叶的光合特性,探索P88S/0389高产的光合机理。[方法]采用W ellburn和Lichenthaler的方法测定剑叶叶绿素及类胡萝卜素含量,并使用便携式光合测定仪测定光合速率及叶绿素荧光参数。[结果]杂交种的叶绿素含量居于双亲之间,生育期前期偏母本而后期偏父本;同时,杂交种有较强的光能吸收、转化和碳固定能力,其剑叶的净光合速率(Pn)、PSⅡ活性(FV/FO)和PSⅡ原初光能转化效率(FV/Fm)、叶绿素荧光光化学猝灭系数(qP)等参数均高于亲本,且下降速率都比亲本慢,非光化学猝灭(qN)较亲本低,上升速率也比亲本慢,表现出不易早衰等超亲优势。[结论]杂交种P88S/0389吸收的光能主要用于光化学反应,热耗散较少。在水稻生产中,选择同时具有较高光合速率和较长的光合速率高值持续期的品种对水稻的高产尤为重要。  相似文献   

播期对不同穗型小麦品种群体动态及部分光合性能的影响     

谷冬艳  尹钧  刘建国  杨宗渠  李金才  屈会娟 《安徽农学通报》2007,13(7):123-127

以两种不同穗型小麦品种为试验材料,研究了播期对小麦群体动态及生育后期群体光合速率、叶绿素荧光动力学参数和产量的影响.结果表明:不同播期对不同穗型小麦品种群体光合、旗叶叶绿素荧光动力学参数及产量的影响存在差异,多穗型小麦品种周麦18在10月上中旬播种,其群体结构、群体光合速率(CAP)、PSⅡ潜在活性FV/Fo、PSⅡ最大光化学效率Fv/Fm、非光化学猝灭系数qN等叶绿素荧光动力学参数较优,最终产量最高;而大穗型品种兰考矮早8在10月19号播种的条件下,上述各性状最优,产量较高.  相似文献   

番茄凝集素荧光光谱学研究     

刘超  汪淑芳  杨瑶君  梁梓  万东海 《安徽农业科学》2008,36(2):401-403

用内源荧光测定和不带电荷的极性淬灭剂丙烯酰胺以及带电荷的I-离子、Cs+对番茄凝集素内源荧光进行淬灭研究,以揭示凝集素与小分子物质相互作用以及凝集素荧光生色基团的分布情况。结果表明,天然番茄凝集素在280nm和295nm激发时均显示λmax为332nm的荧光发射光谱,表明凝集素荧光生色基团位于相对疏水的环境中;中性淬灭剂丙烯酰胺对凝集素分子的荧光生色基团Trp残基的可接近程度为93.41%,而对阳离子淬灭剂Cs+离子的可接近程度为57.14%,阴离子淬灭剂不能淬灭凝集素的内源荧光,表明番茄凝集素荧光氨基酸残基约43%位于分子内部的疏水核心,57%位于分子近表面的疏水袋中,且氨基酸残基周围有大量的碱性氨基酸分布。  相似文献