Map- vs. homology-based cloning for the recessive gene ol-2 conferring resistance to tomato powdery mildew     

Stefano Pavan  Zheng Zheng  Mariya Borisova  Petra van den Berg  Concetta Lotti  Claudio De Giovanni  Pim Lindhout  Hans de Jong  Luigi Ricciardi  Richard G. F. Visser  Yuling Bai 《Euphytica》2008,162(1):91-98

The recessive gene ol-2 confers papilla-associated and race-non-specific resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identified two simple sequence repeat (SSR) markers and one cleaved amplified polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F₂ population to merge these markers with all the ol-2 linked amplified fragment length polymorphism (AFLP^®) markers previously identified in an integrated genetic map. By screening a tomato bacterial artificial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation.  相似文献   

Detection of citrus canker in citrus plants using laser induced fluorescence spectroscopy   总被引：1，自引：0，他引：1  

Emery C. Lins  José Belasque Jr.  Luis G. Marcassa 《Precision Agriculture》2009,10(4):319-330

Citrus canker is a serious disease caused by Xanthomonas citri subsp. citri bacteria, which infects citrus plants (Citrus spp.) leading to a large economic loss in citrus production worldwide. In Brazil citrus canker control is done by an official eradication campaign, therefore early detection of such disease is important to prevent greater economic losses. However, detection is difficult and so far it has been done by visual inspection of each tree. Suspicious leaves from citrus plants in the field are sent to the laboratory to confirm the infection by laboratory analysis, which is a time consuming. Our goal was to develop a new optical technique to detect and diagnose citrus canker in citrus plants with a portable field spectrometer unit. In this paper, we review two experiments on laser induced fluorescence spectroscopy (LIF) applied to detect citrus canker. We also present new data to show that the length of time a leaf has been detached is an important variable in our studies. Our results show that LIF has the potential to be applied to citrus plants.  相似文献   

茶多酚绿色荧光特性研究   总被引：2，自引：0，他引：2  

张丽霞  贾明  夏文娟  向勤锃  黄晓琴 《茶叶科学》2004,24(4):288-294

以茶多酚为材料,研究了茶多酚浓度、溶液pH、溶剂、金属离子（Ca²⁺、Al³⁺）和热处理对茶多酚绿色荧光特性的影响。结果表明：以上各因素对荧光强度的影响都极为显著,当溶液pH=7.4±0.2、茶多酚浓度为3βmg/ml或溶剂采用50%乙醇水溶液时,荧光强度达到最大值;金属离子（Ca²⁺、Al³⁺）的直接影响不明显,主要通过影响游离茶多酚浓度而降低其荧光强度;加热处理能显著增大荧光强度。当茶多酚浓度过高（>3βmg/ml）或pH>8时,能导致最大发射峰位置显著红移,其它因素对其影响较小;此外,溶剂极性显著地影响激发峰的形状和荧光强度,但对最大发射峰位置影响较小。  相似文献   

第7同源染色体组对小麦气体交换及荧光参数的影响     

魏爱丽  畅志坚  王会琴  詹海仙 《安徽农业科学》2010,38(4):2200-2201,2208

[目的]研究7号同源染色体（7A、7B、7D）对小麦光合作用的影响,为今后采用遗传和生理生化手段进行小麦的高光效遗传改良提供一定理论依据。[方法]以山西农科院温室大棚中种植的小麦中国春及其7A、7B、7D染色体缺失植株N7A、N7B、N7D（美国堪萨斯州立大学B.S.Gill教授提供）为材料,于灌浆初期进行相关指标测定,研究小麦7A、7B、7D染色体对小麦光合特性的影响。[结果]N7A、N7B在灌浆初期的光合速率、气孔导度、原初光化学效率（Fv/Fm）、PSⅡ的实际光化学效率（ФPSⅡ）、PSⅡ的表观电子传递速率（ETR）都明显低于中国春,且差异达显著水平（P〈0.05）;N7D的光合速率、气孔导度都明显高于普通小麦中国春,且差异达显著水平（P〈0.05）;Fv/Fm、ФPSⅡ、ETR也高于中国春,但差异不显著。[结论]7A、7B染色体对光合速率有正效应,与气孔导度和光化学反应（荧光参数）均有关系;7D染色体对光合速率具有负效应,而这种负效应主要与气孔导度有关,与光化学反应（荧光参数）无关。  相似文献   

Detection of mast cell degranulation by fluorescent molecular diffusion dynamic measurement     

SUN Yi-ning  SUN Ya-nan  WANG Yu-nong  ZHANG Jing-hai  MA Shu-hua  WANG Yi 《园艺学报》2014,30(9):1717-1723

AIM：To establish a rapid in vitro method for mast cell degranulation tracing by raster image correlation spectroscopy (RICS). METHODS：RBL-2H3, a basophilic granulocyte mast cell line transfected with CD63-GFP plasmid, was used for evaluating the methods, including β-hexosaminidase (HEX) colorimetric assay, scanning electron microscopy (SEM) and RICS in the detection of mast cell degranulation. The sensibilities of these methods were compared. RESULTS：The sensitivities of β-HEX colorimetric assay and SEM were 5 mg/L and 3.9×10-2 mg/L, respectively. RICS detection showed obvious decrease in the diffusion coefficient at dose of 3.9×10-2 mg/L. CONCLUSION：Fluorescent molecular diffusion dynamic measurement can be used for rapid tracing of allergic substances in vitro. According to the results, RICS can achieve nearly the same extent of sensitivity as the SEM does and is far more sensitive than β-HEX colorimetric assay. Compared with SEM, RICS has several advantages: it is faster, simpler and cheaper; it can be used in living cells; it is more suitable for rapid in vitro allergenic compounds tracing. Therefore, RICS is applicable in clinic allergic antigen screening and may also be used in pharmaceutical quality control.  相似文献   

家蚕荧光茧色的研究——Ⅰ.不同品种的荧光茧色   总被引：1，自引：0，他引：1  

陈克平  林昌麒 《蚕业科学》1988,(2)

在紫外光的照射下,观察了348个品种品系的荧光茧色。结果:白茧品种(1)有白荧光、黄荧光、紫荧光及一些中间色的连续光潜。按系统比较白荧光茧的比率是日系>中系>欧系,黄荧光茧的比率是中系>日系>欧系,紫荧光茧的比率是欧系>中系=日系,按化性白荧光茧的比率是日一化>欧一化>多化>中一化四眠>日二化>中二化>中一化三眠,黄荧光茧的比率是中一化三眠>中二化>日二化>多化>中一化四眠>日一化>欧一化,紫荧光茧的比率是欧一化>日一化>中一化四眠>中一化三眠>多化>日二化>中二化。(2)雌雄茧荧光有明显差异,雄黄荧光茧多且强,雌茧紫荧光多。发现了5个品种雄茧基本上为黄荧光,雌茧为紫荧光。(3)荧光茧色与解舒有关系,解舒率与黄荧光茧呈正相关,与紫荧光茧呈负相关,不同的系统,化性则有所区别。有色茧基本上是黄荧光,也有个别品种茧出现白荧光或紫荧光。  相似文献   

Screening and Positioning of Three Chromosome-specific BAC Clones in Gossypium raimondii   总被引：1，自引：1，他引：0  

QIN 　Qin  LIU 　Fang  GAN Yi-Mei  WANG Chun-Ying  WANG Yu-Hong  CAI Xiao-Yan  WANG Kun-Bo 《棉花学报》2013,25(4):323-328

We screened a bacterial artificial chromosome(BAC) library of Gossypium barbadense acc. Pima 90-53 to identify chromosome-specific BAC clones. Using BAC-fluorescence in situ hybridization technology, we obtained three BAC clones specific to chromosome D₅01, D₅02, and D₅10 of Gossypium raimondii, which could be used as cytological markers for those three chromosomes. Comparative mapping of these three BACs between G. barbadense and in G. raimondii showed that these three BAC clones could also be used to identify chromosomes D_b01, D_b02, and D_b10 in G. barbadense. The position of the BAC clone 280G06 on chromosome 10 did not show colinearity between G. barbadense and G. raimondii, possibly because of chromosomal rearrangement.  相似文献   

升高气温对旱柳光合和生长的影响   总被引：7，自引：2，他引：7  

何维明  董鸣 《林业科学》2003,39(1):160-164

工业革命以来 ,大气ＣＯ2 含量增加了 30 % ,预计到 2 1世纪中叶大气ＣＯ2 含量将增加 1倍。与此同时 ,ＣＨ4、Ｎ2 Ｏ、ＣＦＣｓ等温室气体的排放也不断增加 (Ｖｉｔｏｕｓｅｋ ,1 994)。这些温室气体含量的增加必将导致全球性气候变化 (Ｖｉｔｏｕｓｅｋ ,1 994;Ｍｃｌｅｏｄｅｔａｌ.,1 999)。根据大气环流模型 (ｇｅｎｅｒａｌｃｉｒｃｕｌａｔｉｏｎｍｏｄｅｌｓ,ＧＣＭｓ)的预测 ,2 1世纪全球气温将可能平均升高 1 5～ 4 5℃ (Ｈｏｕｇｈｔｏｎｅｔａｌ.,1 996)。在 1 95 1～ 1 989年的约 40ａ间 ,中国半干旱区的平均气温升高了 0 5…  相似文献   

Diagnostic value of FISH combined with multiplex RT-PCR for detection of MLL rearrangement     

HE Yi  LI Xu-dong  WANG Dong-ning  XIAO Ruo-zhi  WANG Wen-wen  HU Yuan  LIN Dong-jun 《园艺学报》2012,28(8):1388-1391

  相似文献   

脱硫废弃物改良盐碱地四翅滨藜荧光特性的研究     

马甜  谢寅峰  徐秀梅 《安徽农业科学》2012,40(14):8147-8149

[目的]为黄河河套地区盐碱地改良及脱硫废弃物资源化利用提供基础资料,摸索一条改良盐碱土的切实可行途径。[方法]在全盐含量12.5 g/kg的重盐碱土上,分别施脱硫废弃物、糠醛渣、改良剂30.0、6.0、1.5 t/hm2,种植四翅滨藜;利用德国WALZ公司生产便携式光合作用-荧光测量系统GFS-3000,测定四翅滨藜叶绿素荧光参数。[结果]与对照比较,施脱硫废弃物改良盐碱地上种植的四翅滨藜植物叶片初始荧光Fo、最大荧光Fm、PSⅡ原初光能转化效率Fv/Fm等均值有所提高;叶片光化学淬灭系数qP、非光化学淬灭系数qN、实际光量子产量的日变化波动幅度有所减小。[结论]施脱硫废弃物可以减弱土壤盐碱胁迫对四翅滨藜叶片光合电子传递活性的影响,增强植物抗逆性,提高光合作用效率,促进植物生长发育。  相似文献