卵泡刺激素受体基因作为产仔数候选基因的研究   总被引：8，自引：1，他引：8  

张淑君  熊远著  邓昌彦  郑嵘  蒋思文  肖森木  夏瑜  徐建祥  刘晓华  王春芳  阮征  宫时玉 《华中农业大学学报》2002,21(6):506-508

分析了FSHR基因2个位点（FSHRA、FSHRB）的PCR－SSCP、PCR－RFLPs多态性，利用SAS软件分析了多态性与母猪产仔数间连锁关系。找到了1个影响产仔数的新的变异位点FSHRB，该位点多态性显著地影响猪产仔数性状，即基因型BB的产仔数显著地高于AB、AA基因型。结果显示出该位点具有作为常规选育的辅助选择的有效的分子标记潜力。  相似文献   

黄颡鱼甘露糖受体基因的克隆和功能分析     

刘小玲  王虹  樊启学  兰江风  林蠡 《水产学报》2017,41(7):1036-1043

甘露糖受体(MR)隶属凝集素超家族,主要表达于巨噬细胞和未成熟的树突状细胞表面。MR不仅在先天免疫防御中发挥重要作用,还通过参与抗原呈递,激活T淋巴细胞,启动获得性免疫应答过程。本研究采用同源克隆技术获得黄颡鱼甘露糖受体(pf MR)基因,采用荧光定量PCR技术检测MR在正常黄颡鱼体内的分布情况,采用鲖爱德华菌感染黄颡鱼头肾巨噬细胞和甘露聚糖封闭MR方法研究黄颡鱼MR在抗细菌感染中的作用。结果显示,pf MR同团头鲂、草鱼、斑马鱼和尼罗罗非鱼的MR聚为一支。pf MR在所检测的12个组织中均有分布,其在肾脏、脾脏和肌肉组织中表达量较高,在血液中表达量较少。鲖爱德华菌感染黄颡鱼头肾巨噬细胞后,pf MR、IL-1β和TNF-a均被细菌诱导表达,超氧阴离子和一氧化氮的含量也上升,超氧阴离子在感染30 min后即显著上升,一氧化氮在感染12 h后才显著上升。甘露聚糖竞争结合MR,显著抑制巨噬细胞内化GFP标签的鲖爱德华菌,加入EDTA减少内化的荧光强度,加入Ca~(2+)使内化的荧光强度回升。研究表明,黄颡鱼头肾巨噬细胞MR参与鲖爱德华菌的识别和内吞过程,而且依赖Ca~(2+)。  相似文献   

海洋贝类Toll样受体及信号通路的研究进展     

乔琨  陈贝  刘智禹  方春华  潘南  许旻  吴靖娜  刘淑集 《福建水产》2016,(6):501-509

Toll样受体(Toll-like receptor,TLR)是模式识别受体家族(PRR)中重要的一员,它可以特异地识别病原体相关的分子模式(PAMP),激活信号级联、诱发固有免疫反应,在抵御病原微生物过程中发挥重要作用。TLRs最早在果蝇胚胎中发现,是一类进化高度保守的免疫受体家族,在果蝇及脊椎动物中研究得较为透彻。海洋贝类具有较高的经济价值及重要系统进化地位,近年来,大量贝类的Toll样受体及信号通路相关因子被发现。本文综述了Toll样受体的结构、信号通路和海洋贝类TLR及信号通路相关因子的研究进展,有助于丰富海洋贝类固有免疫理论研究。  相似文献   

斑节对虾Toll9受体基因免疫功能的研究与表达分析     

刘倩  江世贵  邱丽华  黄建华  周发林  杨其彬  姜松  杨丽诗 《南方水产科学》2017,(5):63-71

Toll受体蛋白(Toll receptors)是一类重要的模式识别受体,在无脊椎动物先天性免疫系统中发挥重要作用。文章对斑节对虾(Penaeus monodon)新型Toll9受体基因(Pm Toll9)进行了研究:以人源胚胎肾细胞(HEK293T)成功构建体外细胞免疫模型,通过免疫印迹方法证实Pm Toll9重组真核蛋白可在HEK293T中成功表达。双荧光素酶报告系统检测发现在200 ng转染浓度处Pm Toll9对NF-κB报告基因的激活效果显著。q RT-PCR数据证明PmToll9成功激活HEK293T细胞Toll-like receptor(TLR)信号通路,促进通路下游髓样分化因子(My D88)、肿瘤坏死因子(TNF-α)和白介素(IL-10)的上调表达,同时酶联免疫吸附测定结果证明Pm Toll9可促进TNF-α蛋白表达水平显著上调。斑节对虾体内细菌刺激实验结果显示无乳链球菌(Streptococcus agalactiae)可激活Pm Toll9在肝胰腺、肠、淋巴和鳃中的表达,哈维氏弧菌(Vibrio harveyi)可显著抑制Pm Toll9在肝胰腺中的表达。提示无乳链球菌可通过Pm Toll9激活Toll信号通路,引起机体免疫防御反应,而哈维氏弧菌对此过程具有一定的抑制作用。  相似文献   

Effect of hydrogen sulfide on myocardial fibrosis and PPARγ and NF-κB expression in rat model of diabetes     

XIAO Ting  LUO Jian  WU Zhi-xiong  LI Fang  ZHANG Jing-jing  YANG Jun 《园艺学报》2015,31(4):635-639

AIM: To explore the effects of hydrogen sulfide (H₂S) on the myocardial fibrosis in a rat model of diabetes and its mechanism.METHODS: Single intraperitoneal injection of streptozotocin (STZ) was utilized to establish a rat model of diabetes. Sodium hydrosulfide was used as an exogenous donor of hydrogen sulfide. Male SD rats were randomly divided into control group, STZ group, STZ+H₂S group and H₂S group. Eight weeks later, HE and VG staining methods were used to observe the collagen distribution and collagen volume fraction was measured by image analysis. The expression levels of type I collagen, PPARγ and NF-κB in the cardiac tissues were determined by Western blotting.RESULTS: Compared with control group, collagen distribution and the expression levels of type I collagen and NF-κB in the cardiac tissues were markedly increased (P<0.05), while PPARγ was significantly decreased in STZ group (P<0.05), but these indexes were reversed significantly in STZ+H₂S group (P<0.05). The expression levels of type I collagen, PPARγ and NF-κB had no significant difference between H₂S group and control group.CONCLUSION: Hydrogen sulfide attenuates cardiac fibrosis in diabetic rats, and its mechanism may be related to PPARγ-NF-κB signaling pathway.  相似文献   

赤点石斑鱼Toll样受体(TLRs)基因家族的鉴定、进化与表达     

陈桂森  曹贞洁  要欣平  魏曹莹  孙云  周永灿 《水产学报》2022,46(5):760-773

为研究赤点石斑鱼Toll样受体(TLRs)的进化、分布及表达模式,本实验基于已公开的赤点石斑鱼基因组和转录组数据,利用Blast、系统进化与共线性等生物信息学方法,分析赤点石斑鱼TLRs基因家族的系统进化、染色体分布及在各组织中的表达模式。结果显示,在赤点石斑鱼中共鉴定出17个TLR基因,这些基因分属于5个亚家族(TLR1、TLR3、TLR5、TLR7和TLR11),分别分布在24条染色体中的11条染色体上;17个TLR基因在赤点石斑鱼不同组织中具有不同的表达模式,然而,位于相同染色体上的TLR基因的不同拷贝则存在相似的表达模式。其中EaTLR18-1与EaTLR18-2均位于9号染色体上,二者的表达模式高度相似,均在心脏中高表达;同位于20号染色体上的EaTLR2-1a与EaTLR2-1b的表达模式也高度相似,均在脑中高表达;EaTLR5M与EaTLR5S同位于14号染色体上,二者不仅具有高度相似的LRR结构域,且在不同组织中的表达水平也高度相似;而位于18号染色体的EaTLR7与EaTLR8和位于4号染色体的EaTLR2-2与EaTLR3,其表达模式却存在明显差异。研究结果可为鱼类...  相似文献   

饲料中添加酵母提取物对凡纳滨对虾免疫相关基因表达及抗菌机能的影响   总被引：2，自引：2，他引：2  

黄旭雄  罗词兴  危立坤  陈春燕  赵利斌  李桑  刘林林  曾蓓蓓 《水产学报》2014,38(12):2049-2058

为了评估饲料中添加酵母提取物对凡纳滨对虾免疫灵敏性和平衡性的相关基因表达的影响,以鱼粉含量为24.5%的基础饲料(对照组)及在基础饲料中添加2.5%酵母提取物的实验饲料,分别饲喂凡纳滨对虾56 d,检测两种饲料投喂的对虾在急性感染溶藻弧菌前后鳃组织Toll受体、IM D和溶菌酶mRNA表达量变化及感染后的对虾死亡情况。结果表明:摄食添加酵母提取物饲料的凡纳滨对虾鳃组织中Toll受体mRNA和溶菌酶mRNA表达量均显著高于对照组对虾(P0.05),IMD mRNA表达量与对照组无显著性差异(P0.05)。急性感染溶藻弧菌后,两组对虾鳃组织Toll受体、IMD和溶菌酶mRNA表达量随感染进程均出现显著变化,Toll受体、IMD和溶菌酶mRNA表达量峰值出现的时间分别为24、42和36 h,且摄食添加酵母提取物组对虾各基因的表达量峰值分别为对照组峰值的201.06%、481.46%和276.77%,均显著高于对照组对虾(P0.05)。摄食添加酵母提取物饲料组对虾鳃组织中Toll受体和IM D mRNA表达量在感染溶藻弧菌后12和24 h分别出现显著上调,而对照组对虾鳃组织中Toll受体和IMD mRNA表达量分别在感染弧菌后24和42 h才出现显著上调。两组对虾经溶藻弧菌人工急性感染后72 h内的累积死亡率无显著差异(P0.05)。实验表明,饲料中添加酵母提取物上调了溶藻弧菌感染前凡纳滨对虾Toll受体和溶菌酶mRNA的表达量,提早了溶藻弧菌感染后对虾Toll受体和IMD mRNA表达量上调时间,且增加了对虾Toll受体、IM D和溶菌酶mRNA表达量的峰值,在一定程度上提高了凡纳滨对虾免疫相关基因表达的灵敏性。  相似文献   

橘色双冠丽鱼黑素皮质素受体1基因(MC1R)整胚原位杂交     

潘贤辉  宋红梅  周康奇  汪学杰  刘奕  牟希东  余梵冬  杨叶欣  刘超  郑曙明  胡隐昌 《水产学报》2019,43(7):1560-1568

为探究黑素皮质素受体1基因(MC1R)在橘色双冠丽鱼胚胎发育和体色形成过程中的表达定位及功能,本研究首先制备了MC1R基因的RNA正反义探针,T7方向转录的正义RNA探针质量浓度为447.529 ng/μL,SP6方向转录的反义RNA探针质量浓度为342.698 ng/μL。经10～20倍稀释后的探针用于原位杂交,MC1R基因探针表达定位显示,随橘色双冠丽鱼胚胎的发育杂交信号总体呈逐渐减弱趋势,原肠期和视泡期其卵黄和胚体侧卧部分有杂交信号分布;出膜期其脊柱、卵黄囊及其内容物和色素细胞内出现杂交信号;出膜期第1天,卵黄表面有信号分布。总体来看,杂交信号在脊索、卵黄囊、卵黄囊内容物质及色素细胞有特异表达,而以正义探针作为阴性对照组在5个胚胎阶段均无任何信号,杂交信号显现的MC1R表达定位说明其在橘色双冠丽鱼色素细胞的分化、迁移中起到重要调控作用,也与神经、营养、免疫功能相关,初步建立了鱼类体色相关基因功能和定位研究的整胚原位杂交方法。  相似文献   

A role for Toll-like receptor 4 in the host response to the lung infection of Yersinia pseudotuberculosis in mice     

《Comparative immunology, microbiology and infectious diseases》2016

Although a Yersinia pseudotuberculosis (Yptb) lung infection model has been developed to study Y. pestis pathogenesis, it is still necessary to establish a new animal model to mimic the pathophysiological features induced by Y. pestis infection. Here, we provide a new lung infection model using the Yptb strain, IP2777, which displayed rapid spread of bacteria to the liver, spleen, and blood. In addition, we examined whether TLR4 is involved in Yptb-induced pathogenesis in the lung infection model of mice we generated. Following lung infection of WT and TLR4-deficient mice with the Yptb strain IP2777, the survival rate, bacterial colonization, histopathology, and level of cytokines and chemokines in the lung, spleen, liver, and blood were analyzed. TLR4-deficient mice had a lower survival rate than WT mice in response to Yptb lung infection. Although the bacterial colonization and pathology of the lung were comparable between WT and TLR4-deficient mice, those of the spleen and liver were more severe in TLR4-deficient mice. In addition, the levels of TNF-α and CXCL2 in the liver and IL-6 and CXCL2 in the blood were higher in TLR4-deficient mice than in WT mice. Our results demonstrate that TLR4 is necessary for optimal host protection against Yptb lung infection and TLR4-deficient mice may serve as a better genetic model of Yptb infection for mimicking Y. pestis infection.  相似文献   

ER-α36 and miR-143 involved in invasion of gastric cancer cells     

ZOU Feng  WANG Xu-ming  LIU Li-jiang 《园艺学报》2012,28(12):2167-2171

AIM: To investigate the relationship between the expression of estrogen receptor α36 (ER-α36) and the invasion of gastric cancer cells. METHODS: SGC7901 cells were treated with 17β-estradiol at high or low concentration. The invasion of gastric cancer cells and the expression of ER-α36 were detected. The SGC7901 cells with the characteristics of stable low expression and high expression of ER-α36 were constructed. The invasion ability and microRNA sequences were determined in above-mentioned recombinant cells. RESULTS: The decreased invasion ability and ER-α36 expression were detected in SGC7901 cells treated with low concentration of 17β-estradiol. The situation was the opposite in the cells treated with high concentration of 17β-estradiol. The expression of miR-143 was significantly decreased in the SGC7901 cells with stable high expression of ER-α36 and was increased in the SGC7901 cells with stable low expression of ER-α36. CONCLUSION: The expression of ER-α36 is positively related to the invasion of gastric cancer cells. It is possible that miR-143 plays an important role in the regulation of gastric cancer invasion.  相似文献