SLC24A5基因影响脊椎动物色素沉积的研究进展   总被引：2，自引：1，他引：1  

曹绣娟  孙守荣  王鑫玉  谭玉文  赵珊  毛华明  邓卫东 《中国畜牧兽医》2008,35(5):52-6

黑色素广泛分布于细菌、真菌、植物和动物体内并扮演了重要的作用。人类浅色皮肤的色素沉积是由于黑色素细胞内黑色素小体数量、大小和密度的减少造成的,而黑色素小体变化也导致斑马鱼由灰色突变为金色,这个金色突变位点编码一个推定的阳离子交换体SLC24A5基因,该基因位于黑色素小体或其前体的细胞膜上。人类和斑马鱼在这个基因的序列及功能上有很高的同源性。该基因的多态性主要出现在非洲和东亚人身上。而等位基因固定在欧洲人的身上,这与局部杂和性大量减少和较浅的皮肤色素沉积有关,表明SLC24A5基因在人类色素沉积方面起关键作用。作者总结了SLC24A5基因与黑色素的研究进展,并简要介绍了乌骨绵羊的发现及其分子特征。  相似文献   

Characterization of virulence properties of Listeria monocytogenes serotype 4b strains of different origins     

Werbrouck H  Botteldoorn N  Ceelen L  Decostere A  Uyttendaele M  Herman L  Van Coillie E 《Zoonoses and public health》2008,55(5):242-248

In this study, the virulence heterogeneity of Listeria monocytogenes serotype 4b strains of different origins was analysed on different levels. On one hand, the survival of L. monocytogenes strains in synthetic gastric fluid was studied. On the other hand, the pathogenic potential of strains with different inlB expression levels was analysed in an A/J mouse model for gastrointestinal listeriosis. Differences in survival capacity in gastric fluid and in in vivo virulence potential were observed between the tested strains. No clear correlation between the origin and the obtained data could be made. However, these results confirm the existence of heterogeneity in virulence potential of L. monocytogenes serotype 4b strains.  相似文献   

Decreased Clinical Severity of Strangles in Weanlings Associated with Restricted Seroconversion to Optimized Streptococcus equi ssp equi Assays          下载免费PDF全文

L. Tscheschlok  M. Venner  K. Steward  R. Böse  M. Riihimäki  J. Pringle 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):459-464

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The impact of direct‐fed microbials and enzymes on the health and performance of dairy cows with emphasis on colostrum quality and serum immunoglobulin concentrations in calves          下载免费PDF全文

S. B. Ort  K. M. Aragona  C. E. Chapman  E. Shangraw  A. F. Brito  D. J. Schauff  P. S. Erickson 《Journal of animal physiology and animal nutrition》2018,102(2):e641-e652

Thirty‐six cows were blocked by calving date and randomly assigned to one of three treatments. Cows were on treatments 3 weeks prepartum through 8 weeks post‐partum. Treatments were as follows: (i) no direct‐fed microbial (DFM) or cellulase and amylase enzymes (C), (ii) 45.4 g/day of DFM (D) or (iii) 45.4 g/day of DFM and 18.2 g/day of enzyme (DE). Total mixed ration fed and refused were measured daily to determine dry matter intake (DMI). Blood samples were taken three times weekly and analysed for β‐hydroxybutyrate, glucose and non‐esterified fatty acids. Body weight (BW) was measured weekly. Colostrum was weighed and analysed for IgA and IgG concentration. Calves were fed 4 L of colostrum within 2 hr of birth. Calf blood samples were taken at 0 and 24 hr for analysis of IgA and IgG concentrations and apparent efficiency of absorption. Milk yield was measured daily and samples collected weekly. Initial BW was different among treatments with D being lesser than C or DE treatments. Body weight, weight gain, efficiency of gain, DMI and blood parameters were unaffected. Treatment did not affect colostrum yield. Ash percentage of colostrum tended to increase with D and DE, while IgA and total solids yield decreased with D. Colostrum fat yield was decreased in D and DE. Treatments did not impact BW, serum IgA and IgG concentrations or apparent efficiency of absorption of calves. Post‐partum BW, DMI, blood parameters, milk production and composition were unaffected by treatment. However, cows on D gained more BW and tended to have greater efficiency of gain compared to those on DE, but were similar to C. Somatic cell scores were greatest for D. Results indicate that DFM and enzyme supplementation did not improve health and performance of dairy cattle during the pre‐ and post‐partum periods under conditions of this study.  相似文献   

Identification of hypoglycin A binding adsorbents as potential preventive measures in co‐grazers of atypical myopathy affected horses          下载免费PDF全文

T. Krägeloh  J. M. V. Cavalleri  J. Ziegler  J. Sander  M. Terhardt  G. Breves  A. Cehak 《Equine veterinary journal》2018,50(2):220-227

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Gene expression profiling in embryonic chicken ovary during asymmetric development          下载免费PDF全文

Amir Hossan Shaikat  Shoko Namekawa  Sadequllah Ahmadi  Misa Takeda  Takeshi Ohkubo 《Animal Science Journal》2018,89(4):688-694

The reproductive system in female birds arises as bilateral asymmetrical anlagen, excluding the birds of prey. Earlier, histological and messenger RNA (mRNA) expression profile studies of several genes related to gonadal sex differentiation in chicken embryos tried to elucidate the query of this asymmetry in a scattered manner. To understand the matter precisely, we have focused on mRNA expression of a cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) in second half of the embryonic days (E10–E18). The established role of leptin in development of the embryo and its expression in the embryonic ovary also drove us to check leptin receptor (LEPR) expression in the ovary. Increased expression of FSHR and CYP19A1 in the left ovary compared with that in the right ovary was identified (P < 0.05), promoting preferential left ovarian development and functionality. Significant high expression (P < 0.05) of the apoptotic genes in the right ovary were also involved here. Leptin probably has no direct influence on ovarian asymmetry as no significant variation in gonadal mRNA expression of LEPR was observed within the same experimental days. We propose that asymmetric expression of this cohort of genes (FSHR, CYP19A1, caspase 3, caspase 8) leads to the development of dimorphic gonads during embryogenesis.  相似文献   

龟致病性维氏气单胞菌和鲍曼不动杆菌双重PCR检测方法的建立     

丁利  代小梅  方振华  汪继超  史海涛 《水产科学》2016,(5):587-590

应用2对特异性引物,通过对反应条件和体系的优化,成功建立了能快速检测维氏气单胞菌和鲍曼不动杆菌两种病原的双重PCR方法。研究结果表明,该方法特异性强,仅对龟鳖维氏气单胞菌和鲍曼不动杆菌有扩增,而对其他龟鳖常见病原无扩增;该方法对维氏气单胞菌最低检测核酸质量浓度为1.75×10-3 ng/μL,对鲍曼不动杆菌最低检测核酸质量浓度为3.96×10-3 ng/μL,检出率较传统检测方法高。该研究所建立的双重PCR方法灵敏度高、特异性好,可为临床龟鳖维氏气单胞菌和鲍曼不动杆菌感染的诊断和流行病学调查等提供帮助。  相似文献   

橡胶树HbFCA启动子的克隆及其在橡胶树中的表达分析     

华玉伟  孙芳  黄天带  蔡海滨  杨加伟  黄华孙 《热带作物学报》2013,34(5):800-806

FCA(Flowering control locus A)基因是自主开花调控途径中的关键调控基因之一,同时,FCA功能具有多样性.本研究中,利用TAIL-PCR方法获得2039 bp橡胶树FCA基因的启动子序列,生物信息学分析发现该启动子中具有28种顺式调控元件,其中主要为基因表达调控核心元件(TAAT-box和CAAT-box)和光响应调控元件.此外,还含有激素、胁迫信号和光周期的顺式作用元件.同时,将橡胶树FCA启动子与GUS基因融合构建植物表达载体转化橡胶树,发现在胚状体、叶片和树根中GUS基因均强烈表达,但在茎段中表达较弱,说明橡胶树FCA基因除参与开花调控外,还可能参与多种发育过程的调控.本研究获得的HbFCA启动子转化橡胶树植株为进一步分析Hb FCA基因功能及表达调控模式提供了遗传材料.  相似文献   

Association of rs2162459 polymorphism in CYP7A1 gene with effectiveness of atorvastatin in northern Chinese Han population     

WANG Guo-chang  CHEN Xiao-ming  LI Shu-yuan  ZHANG Na-na  ZHENG Li-juan  CHEN Tian-tian  ZHUANG Mao-qiang  ZHANG Lin  CHEN Huan-qin  L Ming 《园艺学报》2013,29(8):1365-1369

AIM：To investigate the association of the polymorphism of rs2162459 locus in cytochrome P-450, family 7, subfamily A, polypeptide 1 (CYP7A1) gene, encoding cholesterol 7α-hydroxylase, with the effectiveness of atorvastatin in northern Chinese Han population. METHODS：Clinical data and blood samples of 200 cases of hyperlipidemia patients were collected. The variants of rs2162459 in CYP7A1 gene were detected by multiplex SNaPshot technology. Several genetic models were constructed to analyse the association of gene polymorphism with the effectiveness of atorvastatin by logistic regression method. RESULTS：The polymorphism of rs2162459 was consistent with Hardy-Weinberg equilibrium. The baseline high-density lipoprotein cholesterol (HDL-C) concentrations in three genotypes of AA, GA and GG were (136±0.94) mmol/L, (1.16±0.38) mmol/L and (1.07±0.28) mmol/L, respectively (P<0.05). There were differences in the genotypic frequency and allelic frequency between atorvastatin effective and ineffective groups (both P<005). Significant differences in regulating HDL-C level among the three genotypes of rs2162459 were found after logistic regression. The results of additive model, generalized model and dominant model, presented as OR (95% CI), were 1.74 (1.09~2.77), 2.86 (1.13~7.25) and 2.21 (1.12~4.33), respectively. CONCLUSION: The baseline HDL-C level in the carriers of GG genotype is lower than that in the carriers of the other two genotypes. The HDL-C-elevating effect of atorvastatin on GG genotype carriers is more significant than that on AA genotype carriers.  相似文献   

早熟欧李新品种‘夏日红’的选育(英文)     

姚砚武  兰彦平  彭薇  李永利 《农业科学与技术》2017,18(1)

‘夏日红’是2006年从自然杂交实生后代中选育而成的早熟型欧李新品种。果实扁圆形,果皮红色,果肉黄色,味酸甜;平均单果质量6.74 g,半离核。果实可溶性固形物含量达10.9%,总糖含量8.20%,总酸含量1.81%,糖酸比4.53,可食率94.51%。果肉含钙219.7 mg/kg,铁4.4 mg/kg,锌1.09 mg/kg,维生素C 182 mg/kg。果实发育期96~101 d,北京地区7月下旬成熟;货架期3~5 d,冷藏20 d左右,冷冻可达1a,果实不失味。适合北京地区栽培,第二年开花结果,丰产性较强。  相似文献