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11.
The cross resistance of four resistant strains of Liposcelis bostrychophila Badonnel, an important stored-product insect pest, was investigated. These four strains included the HCO2-R (hypercarbia-resistant strain), HCLO-R (hypoxia and hypercarbia resistant strain),DDVP-R (DDVP-resistant strain) and PH3-R (PH3-resistant strain). The results indicated that there were cross-resistances between CA and insecticides, and the quantities of the cross-resistance were different. The cross-resistance factor (RF) of HCO2-R to hypoxia and hypercarbia was 3.2458, whilst, that of HCLO-R to hypercarbia was 1.8280. The RF of DDVP-R to PH3 was 3.9614, whilst, that of PH3-R to DDVP was 2.7852. The RF values of DDVPR and PH3-R to hypercarbia were 1.3550 and 1.1816, respectively. However, the RF of HCO2-R to DDVP was 2.1372. There also was a low cross-resistance between DDVP-R and HCO2-R. The RF of HCO2-R to PH3 was 3.3698. This sugqested that the insects resistant to high CO2concentration atmosphere would develop significant resistance to PH3. However, the insects resistant to PH3 remain sensitive to hypercarbia atmosphere. Both DDVP-R and PH3-R developed resistance to high CO2 treatment. There was a low cross-resistance between DDVP-R and HCLO-R. The insects resistant to hypoxia and hypercarbia were very sensitive to DDVP. There also was cross-resistance between HCLO-R and PH3-R, but the resistance of PH3-R to hypoxia and hypercarbia was lower than that of HCLO-R to PH3. The difference of the overlapping and separate values indicated that there were differences in the intercross-resistance of four resistant strains. 相似文献
12.
[目的]首都机场检疫局从入境旅客携带物上截获一种啮虫目昆虫,经中国农业大学植物检疫与入侵生物学实验室鉴定为嗜卷书虱Li poscelis bostrychophila Badonnel,1931;[方法]对该虫的有关文献的翻译和整理,对该虫进行了全面的介绍;[结果]详细介绍了其分类地位、地理分布、生物学特性、传播情况和潜在危害等,并提供了10个近缘种的检索表;基于以往截获记录、口岸鉴定及实际防治中存在的困难,讨论了仓储啮虫的检疫意义;[结论]鉴于仓储啮虫危害特点及其检疫意义,口岸应引起足够的重视,加强检疫工作,严防该类害虫的传入. 相似文献
13.
Wei Dou 《Pesticide biochemistry and physiology》2009,94(1):10-14
Glutathione S-transferases (GSTs) catalyzing the conjugation of reduced glutathione (GSH) to a vast range of xenobiotics including insecticides were investigated in the psocid Liposcelis bostrychophila Badonnel. GSTs from susceptible and two resistant strains (DDVP-R for dichlorvos-resistant strain and PH3-R for phosphine-resistant strain) of L. bostrychophila were purified by glutathione-agarose affinity chromatography and characterized by their Michaelis-Menten kinetics towards artificial substrates, i.e., 1-chloro-2,4-dinitrobenzene (CDNB), in a photometric microplate assay. The specific activities of GSTs purified from two resistant strains were significantly higher than their susceptible counterpart. For the resistant strains, GSTs both showed a significantly higher affinity to the substrate GSH while a declined affinity to CDNB than those of susceptible strain. The inhibitory potential of ethacrynic acid was very effective with highest I50 value (the concentration required to inhibit 50% of GSTs activity) of 1.21 μM recorded in DDVP-R. Carbosulfan also exhibited excellent inhibitory effects on purified GSTs. The N-terminus of the purified enzyme was sequenced by Edman degradation, and the alignment of first 13 amino acids of the N-terminal sequence with other insect GSTs suggested the purified protein was similar to those of Sigma class GSTs. 相似文献
14.
测定了磷化氢、敌敌畏、大蒜油和二氧化碳及其联用对嗜卷书虱(Liposcelis bostrychophila Badonnel)成虫的熏蒸活性。4种药剂对试虫的致死作用随剂量的增加而增强。4种药剂对试虫的熏蒸活性从高到低依次为:磷化氢敌敌畏大蒜油二氧化碳,其LC50分别为0.33mg·L-1、0.54mg·L-1、1.60mg·L-1和259.08mg·L-1。4种药剂联用时,正交试验结果显示4种药剂对试虫死亡率的影响从大到小依次为:大蒜油、敌敌畏、二氧化碳和磷化氢。1.32mg·L-1大蒜油0.52 mg·L-1敌敌畏14%二氧化碳0.20mL·L-1磷化氢联用时具有最佳的杀虫效果。结果表明联用药剂的效果优于单一药剂,且在嗜卷书虱的防治中有较强的应用价值。 相似文献
15.
Chun Xue You Hai Yan Jiang Wen Juan Zhang Shan Shan Guo Kai Yang Ning Lei Ping Ma Zhu Feng Geng Shu Shan Du 《Journal of insect science (Online)》2015,15(1)
The essential oil of Clausena anisum-olens (Blanco) Merr. showed strong contact toxicity and repellency against Lasioderma serricorne and Liposcelis bostrychophila adults. The components of the essential oil obtained by hydrodistillation were determined by gas chromatography-mass spectrometry. It was found that the main components were myristicin (36.87%), terpinolene (13.26%), p-cymene-8-ol (12.38%), and 3-carene (3.88%). Myristicin and p-cymene-8-ol were separated by silica gel column chromatography, and their molecular structures were confirmed by means of physicochemical and spectrometric analysis. Myristicin and p-cymene-8-ol showed strong contact toxicity against L. serricorne (LD50 = 18.96 and 39.68 μg per adult) and Li. bostrychophila (LD50 = 20.41 and 35.66 μg per adult). The essential oil acting against the two grain storage insects showed LD50 values of 12.44 and 74.46 μg per adult, respectively. Myristicin and p-cymene-8-ol have strong repellent toxicity to Li. bostrychophila. 相似文献
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17.
为防控暗褐书虱Liposcelis brunnea入侵和扩散,以mtDNA COI基因为靶标设计环介导等温扩增(loop-mediated isothermal amplification,LAMP)特异性内外引物,测定引物的特异性和灵敏度,并对暗褐书虱DNA粗提液进行LAMP可视化鉴定检测。结果显示,所设计的暗褐书虱LAMP引物特异性良好,DNA浓度的检测灵敏度为1.0×10-2ng/μL;在65℃下恒温反应45 min后,暗褐书虱LAMP反应体系呈黄色(阳性反应),而其他啮虫昆虫的LAMP反应体系均呈紫红色(阴性反应),以DNA粗提液和以试剂盒法提取的DNA的LAMP可视化鉴定检测结果相同,但鉴定时间缩短到了1 h内,表明所建立的LAMP可视化鉴定技术高效、灵敏、操作简单,可快速准确地鉴定暗褐书虱。 相似文献
18.
嗜虫书虱的有效积温及气调致死作用研究 总被引：3，自引：0，他引：3
嗜虫书虱以全面粉及酵母粉饲养时，各虫态发育速率与温度呈逻辑斯蒂曲线关系，高浓度的ＣＯ２是导致该虫死亡的主要因子，其次是温度和Ｏ２浓度。在不影响储粮品质下，适当升高仓内温度，并配以高浓度的ＣＯ２和低浓度的Ｏ２是控制该虫的最佳组合因子。 相似文献
19.
Numerous strains of the psocid pest, Liposcelis decolor (Pearman) were collected from farms and central storages and interbred to form three representative strains from three major grain-growing states of Australia: Queensland, South Australia and New South Wales. These were tested against the grain protectants and structural treatments currently registered for use in Australia. Recently, L decolor has become an important pest of stored grain in Australia, particularly in the eastern and southern parts. There is no published information available on management of this pest and the current pest-management strategy, based predominantly on phosphine fumigation, has failed to control infestations of this pest in numerous grain storages in Australia. Alternative methods of control such as use of contact insecticides were explored in the present work to supplement phosphine fumigation to manage this new pest. From eight grain protectants tested as admixtures, only chlorpyrifos-methyl, bioresmethrin plus piperonyl butoxide, and fenitrothion were found to provide long term (3-9 months) protection against all three strains of L decolor. Chlorpyrifos-methyl gave the best protection, providing a minimum of 7.5 to a maximum of 9 months protection, depending on the strains tested. Three structural treatments, azamethiphos, azamethiphos plus carbaryl and permethrin provided long-term control (8-9 months) of all three strains of L decolor on galvanised steel surfaces, with permethrin delivering 9 months protection against all strains. However, all of these treatments failed to provide long-term control of any strains on concrete surfaces. We conclude that chlorpyrifos-methyl as a grain admixture can be incorporated into fumigation strategies to optimise the control of L decolor infestations. Structural treatments, such as permethrin can be used to support a fumigation strategy in storages made of galvanised steel. 相似文献
20.
采用饲料混毒法系统测定了吡丙醚和烯虫酯两种昆虫生长调节剂(IGRs)对重要的储藏物害虫嗜卷书虱Liposcelis bostrychophila Badonnel若虫和成虫的致死作用,对若虫发育情况、成虫繁殖力和卵孵化率的影响。结果表明,以吡丙醚和烯虫酯10 mg/kg的混毒饲料饲喂嗜卷书虱2龄幼虫49 d后,若虫的死亡率分别为80.00%和31.11%;取食20、10和5 mg/kg吡丙醚的若虫最长存活天数分别为71.0、98.2和116.4 d,而对照仅11.3 d即全部羽化为成虫;取食20 mg/kg以下烯虫酯混毒饲料的若虫一部分仍可羽化成成虫,而且成虫仍可繁殖后代;取食吡丙醚混毒饲料的若虫,60 d后存活下来的若虫大多以超龄若虫的形式存在,而取食烯虫酯的若虫则很少出现超龄若虫。成虫取食烯虫酯(200 mg/kg)和吡丙醚(40 mg/kg)混毒饲料后7 d,死亡率分别为26.67%和23.33%,成虫产卵量分别是对照的39.77%和26.13%,产卵后15 d,卵的孵化率分别是65.71%和8.69%,而对照仅为93.18%。该项研究表明,采用40 mg/kg吡丙醚和200 mg/kg的烯虫酯对嗜卷书虱虫口数量有明显的控制作用。 相似文献