Molecular mapping of a dominant genic male sterility gene Ms in rapeseed (Brassica napus)   总被引：7，自引：1，他引：7  

G. Y. Lu    G. S. Yang  T. D. Fu 《Plant Breeding》2004,123(3):262-265

Rs1046AB is a genic male sterile two‐type line in rapeseed that has great potential for hybrid seed production. The sterility of this line is conditioned by the interaction of two genes, i.e. the dominant genic male sterility gene (Ms) and the suppressor gene (Rf). The present study was undertaken to identify DNA markers for the Ms locus in a BC₁ population developed from a cross between a male‐sterile plant in Rs1046AB and the fertile canola‐type cultivar ‘Samourai’. Bulked segregant analysis was performed using the amplified fragment length polymorphism (AFLP) methodology. From the survey of 480 AFLP primer combinations, five AFLP markers (P10M13₃₅₀, P13M8₄₀₀, P6M6₄₁₀, E7M1₂₃₀ and E3M15₁₀₀) tightly linked to the target gene were identified. Two of them, E3M15₁₀₀ and P6M6₄₁₀, located the closest, at either side of Ms at a distance of 3.7 and 5.9 cM, respectively. The Ms locus was subsequently mapped on linkage group LG10 in the map developed in this laboratory, adding two additional markers weakly linked to it. This suite of markers will be valuable in designing a marker‐assisted genic male sterility three‐line breeding programme.  相似文献   

遗传连锁方法的历史,现状与发展     

宋九洲  张沅 《中国牛业科学》1995,21(1):1-3,13

本文简要介绍了遗传学当中的连锁分析方法的历史，现状及其近几十年的发展，并对这些方法的优缺点进行了评价。  相似文献   

三维碎片拼合的轮廓提取和特征点检测     

朱延娟  周来水王坚 《勤云标准版测试》2005,(1):23-29

针对三维空间碎片的拼接,提出一种轮廓提取和特征点检测的方法。首先,采用一种简单、有效的方法来构建三维碎片数据的拓扑关系,并在此基础上提取碎片的轮廓曲线。对存在缺陷、拓扑关系难以建立的三维测量数据,中给出了相应的修改、调整方法。同时,提出了一种对轮廓进行重采样的方法来计算空间轮廓上每一点的曲率和挠率。将曲率和挠率相结合得出轮廓曲线上各点的全曲率,然后由全曲率值来检测碎片轮廓的特征点。轮廓曲线的描述就由其上每一点的曲率和挠率以及特征点来表征。实验表明了该算法的有效性和稳定性。  相似文献   

以标记辅助选择改良江苏主栽粳稻品种“淮稻5号”黑条矮缩病抗性   总被引：1，自引：0，他引：1  

李爱宏  潘存红  戴正元  肖宁  余玲  李育红  张小祥  张洪熙  潘学彪 《作物学报》2012,38(10):1775-1781

水稻黑条矮缩病是由灰飞虱传播的一种病毒病,最近在江苏、浙江等省暴发给水稻生产造成严重威胁。本研究在前期对水稻黑条矮缩病抗性基因初步定位的基础上,针对第6染色体短臂上紧密连锁的两个抗性QTL,以抗性亲本“明恢63”为供体,感病亲本“淮稻5号”为轮回亲本,通过标记辅助选择,构建了携带目标抗性QTL的系列近等基因系。自然接种和人工接种鉴定表明新培育的近等基因系其发病率较对照轮回亲本下降25%左右,抗性得到显著改良。农艺性状调查结果显示大多数近等基因系其生育期、株高及产量构成性状等与轮回亲本相似,表明目标区段存在较少的累赘连锁。此外,对有关如何实现目标QTL的精细定位,以及利用标记辅助选择聚合多个抗性QTL培育抗病品种的策略也进行了探讨。  相似文献   

Development of SCAR and CAPS Markers for a Partially Dominant Yellow Seed Coat Gene in Brassica Napus L     

Zhiwen Liu  Tingdong Fu  Ying Wang  Jinxing Tu  Baoyuan Chen  Yongming Zhou  Chaozhi Ma  Lianmin Shan 《Euphytica》2006,149(3):381-385

Summary We have previously identified 2 RAPD and 8 AFLP markers that were associated with yellow seed coat gene in a stable and pure yellow-seeded DH line No. 2127-17, however, they were not suitable in large-scale MAS. In this paper, it reported our efforts in developing rapid and reliable SCAR and CAPS markers from the RAPD and AFLP markers. Based on the sequence information in the regions of the most closely linked 4 AFLP and 2 RAPD markers, one SCAR (SCS1130) and one CAPS (SCA1) maker were successfully developed. Linkage analysis on a 127 DH lines population derived from the cross Hui5148-2 × No. 2127-17 revealed that they were tightly flanked (3.2 cM, 3.8 cM respectively). The resulting SCS1130 and SCA1 were further evaluated in backcross breeding families and demonstrated more accurate and valuable in MAS breeding. The development of these new markers would facilitate and accelerate the yellow seed coat breeding in Brassica napus.  相似文献   

QTL analysis for early-maturing traits in cotton using two upland cotton (Gossypium hirsutum L.) crosses     

Chengqi Li  Xiaoyun Wang  Na Dong  Haihong Zhao  Zhe Xia  Rui Wang  Richard L. Converse  Qinglian Wang 《Breeding Science》2013,63(2):154-163

Making use of the markers linked closely to QTL for early-maturing traits for MAS (Marker-assisted selection) is an effective method for the simultaneous improvement of early maturity and other properties in cotton. In this study, two F₂ populations and their F_2:3 families were generated from the two upland cotton (Gossypium hirsutum L.) crosses, Baimian2 × TM-1 and Baimian2 × CIR12. QTL for early-maturing traits were analyzed using F_2:3 families. A total of 54 QTL (31 suggestive and 23 significant) were detected. Fourteen significant QTL had the LOD scores not only > 3 but also exceeding permutation threshold. At least four common QTL, qBP-17 for bud period (BP), qGP-17a/qGP-17b (qGP-17) for growth period (GP), qYPBF-17a/qYPBF-17b (qYPBF-17) for yield percentage before frost (YPBF) and qHFFBN-17 for height of first fruiting branch node (HFFBN), were found in both populations. These common QTL should be reliable and could be used for MAS to facilitate early maturity. The common QTL, qBP-17, had a LOD score not only > 3 but also exceeding permutation threshold, explaining 12.6% of the phenotypic variation. This QTL should be considered preferentially in MAS. Early-maturing traits of cotton are primarily controlled by dominant and over-dominant effects.  相似文献   

猪育种中DNA标记辅助选择方法的研究进展   总被引：1，自引：0，他引：1  

王重龙  陶立 《中国畜牧兽医》2008,35(2):42-46

DNA标记辅助选择（简称MAS）为猪的育种提供了一个新的突破性方法,作者将从MAS的原理和实施方案、猪的基因图谱、猪育种中的若干重要DNA标记、MAS应用效果的理论研究和MAS应用实践等方面,综述其在猪育种中的应用研究进展。  相似文献   

Improvement of diagnostic markers for resistance to Globodera pallida and application for selection of resistant germplasms in potato breeding     

Kenji Asano  Etsuo Shimosaka  Yoko Yamashita  Takashi Narabu  Satoshi Aiba  Itaru Sakata  Kotaro Akai  Satoshi Okamoto  Seiji Tamiya 《Breeding Science》2021,71(3):354

Occurrence of pale potato cyst nematode, Globodera pallida (Stone) Behrens, was first recorded in Japan in 2015. Among several control measures, cultivation of resistant potato (Solanum tuberosum L.) varieties is the most effective in cost and environmental impact. As no G. pallida-resistant varieties have yet been developed in Japan, great emphasis is being placed on screening of germplasm possessing the resistance and development of the resistant varieties. In this study, we first improved previously reported DNA markers linked to the G. pallida resistance loci (GpaIV^s_adg and Gpa5) and then used these to screen more than 1,000 germplasms to select several candidate germplasms with resistance. We performed inoculation testing on the selected candidates and identified several resistant germplasms to the Japanese G. pallida population. Furthermore, we developed a simultaneous detection method combining three DNA markers linked to G. pallida and Globodera rostochiensis (Wollenweber) Behrens resistance loci. We validated the ability of C237-I marker to select resistant allele of GpaIV^s_adg and predict the presence of resistance in a Japanese breeding population. Resistant germplasms identified in this study could potentially be used to develop G. pallida-resistant varieties. The marker evaluation methods developed in this study will contribute to the efficient development of resistant varieties.  相似文献   

基于MAS的生态工业系统信息模型     

李健  魏增强 《中国农机化》2010,(4):49-52,41

根据主体特征,将生态工业系统划分为四项核主体,通过引入一种适合开放环境的Agent体系结构,针对核心主体抽象出四项主体agent。以四项主体Agent模拟的多主体系统对生态工业系统的运作建立信息模型,通过模型信息反映生态工业系统运行的内在机制与动态演化,在此基础对生态工业园的建设进行剖析,为生态工业的推广提供指导。  相似文献   

利用分子标记辅助选择技术培育抗虫水稻品种的研究     

赵越  张石来  胡建  黄立钰  张静 《中国农业科技导报》2016,18(3):25-31

Bt基因是从苏云金芽孢杆菌中克隆出的针对鳞翅目害虫的抗虫基因,经过基因工程方法人工改造已经获得Cry1Ab/Ac、Cry1C等抗虫基因。以云南主栽水稻品种楚粳28为受体,以携带Bt抗虫基因的"华恢1号"、"RJ-5"和"T1C-19"分别作为供体,利用分子标记辅助选择(MAS)技术进行回交育种,选育成携带Bt抗虫基因水稻新品系。结合农艺性状表现,获得了云抗虫稻1号、云抗虫稻2号、云抗虫稻3号Bt蛋白高表达的水稻抗虫品系。这些抗虫品系为云南省抗虫水稻的遗传改良和生产应用提供了基因资源和应用基础。  相似文献