稻瘟菌侵染诱导的水稻过氧羟基脂肪酸羟化环氧化酶的抗血清制备     

苏生昌  张淑平  范军 《植物病理学报》2000,30(3):207-212

 通过选择性抽提、盐析、阴阳离子交换柱层析等步骤从非亲和性稻瘟菌侵染的120g稻叶中获得了诱导性过氧羟基脂肪酸羟化环氧化酶(POG)的电泳纯蛋白250μg。利用这些纯化蛋白,制备了POG的抗血清。点免疫杂交结果表明,该抗血清效价大于1:5000,其抗原检测灵敏度可达0.05ng,并具有较好的特异性。蛋白印迹结果显示,稻瘟菌侵染可诱导POG在水稻细胞膜上的累积。  相似文献   

水稻白叶枯病菌转录调控因子OxyRxoo对细菌抗氧化系统的调控作用     

刘田田 《安徽农业科学》2011,39(24):14542-14544

病原细菌对活性氧的降解是抗氧化和侵染中的重要毒性机制之一。阐述了水稻白叶枯病菌（Xanthomonas oryzaepv.oryzae）的转录调控因子OxyRxoo在病菌抗氧化系统中的调控作用,以及目前对OxyRxoo的研究进展。  相似文献   

稻瘟病菌浅黄色素突变体对稻株诱导抗性的探讨（英文）     

沈瑛  黄大年 《中国水稻科学》1990,4(3):139-142

经用紫外光诱变菲律宾的一个具有广谱毒性的菌株PO6-6中获得2个浅黄色素突变株A和B以及从浙江省分离的弱致病性菌株C,在25/16℃、RH70%的人工气候箱内,先在4个感病品种上接种48小时后再接种强致病性菌株D和E,浅黄色素突变株和弱菌株对强菌株在水稻感病植株上呈现出交叉保护现象。6批重复试验一致表明,浅黄色素突变株和弱致病性菌株均可明显地诱导水稻植株对稻瘟病的抗病性。本文并就利用稻瘟病菌的交叉保护作用,为开辟防治新途径进行了讨论。  相似文献   

Identification of a major blast resistance gene in the rice cultivar 'Tetep'   总被引：1，自引：0，他引：1  

S. R. Barman    M. Gowda    R. C. Venu  B. B. Chattoo 《Plant Breeding》2004,123(3):300-302

Analysis of near‐isogenic lines (NILs) indicated the presence of a novel resistance gene in the indica rice cultivar ‘Tetep’ which was highly resistant to the rice blast fungus Magnaporthe grisea.‘Tetep’ was crossed to the widely used susceptible cultivar ‘CO39’ to generate the mapping population. A Mendelian segregation ratio of 3 : 1 for resistant to susceptible F₂ plants further confirmed the presence of a major dominant locus, in ‘Tetep’, conferring resistance to the blast fungal isolate B157, corresponding to the international race IC9. Simple sequence length polymorphism (SSLP) was used for molecular genetic analysis. The analysis revealed that the SSLP marker RM 246 was linked to a novel blast resistance gene designated Pi‐tp(t) in ‘Tetep’.  相似文献   

杂交稻野败胞质对稻瘟病菌、白叶枯病菌感病性的研究   总被引：9，自引：0，他引：9  

杨仁崔  卢浩然 《作物学报》1989,15(4):310-318

选用3个水稻野败胞质不育系及相应的保持系,3个恢复系及它们相应的野败胞质代换系,这些不育系、保持系同恢复系配组的18个 F_1,分别在秧苗期接种属我国7个稻瘟病生理小种群的21个菌株;在成株期接种15个白叶枯病菌株。结果表明,野败胞质较之正常胞质表现对这两种病菌感病稍轻;这种差异在多数保持系的基因型背景下达到显著水准,  相似文献   

Partial resistance to neck blast influenced by stage of panicle development and rice genotype     

E. C. Roumen 《Euphytica》1992,64(3):173-182

Summary Neck nodes of eight rice genotypes were inoculated with a virulent isolate of the blast pathogen at four slightly different, increasingly older stages of panicle development shortly after flowering. Resistance to infection as well as resistance to growth of the pathogen after infection was assessed. Significant differences between genotypes were found for both components of resistance. Resistance to growth of the pathogen sharply increased with aging of the neck node as was indicated by a large reduction of the length of the lesions on the culm, but resistance to infection did not change much. The lesion length was closely related to yield loss. A small delay in time of infection can lead to a large decrease in yield reduction. Meaningful comparison of partial resistance to neck blast between genotypes therefore requires infection at an identical stage of panicle development. In the field, where panicles are in different stages of development, selection of genotypes that do show the symptoms of neck blast together with relatively little yield loss in infected panicles is recommended.  相似文献   

Genetic Analysis of Resistance to Xanthomonas campestris pv. oryzae (Ishiyama) Dye in a Rice Breeding Line ARC 10464     

R. P. Singh    A. K. Gupta  R. G. Saini 《Plant Breeding》1992,108(4):335-337

The genetics of resistance to three Indian pathotypes of Xanthomonas campestris pv. oryzae, namely, IX01, IX08 and IX09, was studied in a landrace of Indica rice ‘ARC 10464’. Resistance to each of the two pathotypes IX01 and IX09 was governed by two independently-inherited dominant genes while a single dominant gene was operative against patho-type 1X08. The joint segregation tests conducted on F₂ plant progenies (F₃ families) using pathotypes IX01, IX08 and IX09 suggested that the gene/(s) effective against each of the pathotypes are different.  相似文献   

A high-resolution map of the rice blast resistance gene Pi15 constructed by sequence-ready markers     

F. Lin    Y. Liu    L. Wang    X. Liu    Q. Pan 《Plant Breeding》2007,126(3):287-290

The gene Pi15 for resistance of rice to Magnaporthe grisea was previously mapped to a ≈0.7-cM region on chromosome 9. To further define the chromosomal region of the Pi15 locus, a contig spanning the locus was constructed, in silico , through bioinformatics analysis using a reference sequence of the cultivar 'Nipponbare'. One simple sequence repeat marker adopted from the International Rice Microsatellite Initiative and six candidate resistance gene (CRG) markers, developed from gene annotation of the reference sequence of the contig, were used for linkage analysis in a mapping population consisting of 504 extremely susceptible F₂ plants. The Pi15 locus was delimited to a ≈0.5-cM region flanked by the markers CRG5 and CRG2 and co-segregated with the markers BAPi15₇₈₂, CRG3 and CRG4, which was physically converted to a 44-kb interval.  相似文献   

用不同类型的水稻鉴别品种鉴定江苏稻瘟病菌的致病性   总被引：11，自引：0，他引：11  

周益军  程兆榜  范永坚  王金生  陈毓苓  张文荟 《作物学报》2003,29(2):268-273

根据DNA指纹图谱和系谱, 从1999年采集的96个江苏稻瘟菌株中选择44个菌株进行致病性测定. 它们被鉴定为7个生理小种. 用日本清泽系列单基因鉴别品种和LTH近等基因系(NILs)鉴别品种分析, 分别产生27和10个致病类型, CO39 NILs对江苏菌株几乎没有鉴别作用. 分析结果可见供试菌株之间致病性差异极大. 研究表明, 发展包含较多抗病  相似文献   

2007-2008年江苏省稻瘟病菌遗传多样性   总被引：1，自引：0，他引：1  

杨豪  任春梅  陈毓苓  范永坚  程兆榜  周益军 《华北农学报》2011,26(4):135-140

利用致病性测定及Pot2-rep-PCR技术对2007、2008年采集自江苏省五大稻区的稻瘟病菌株进行多样性分析,这2年的菌株被划分为10个小种和14个系谱,其中ZG1为优势小种,JSL5为优势系谱,研究结果表明:江苏稻瘟病菌具有丰富的多样性,但与1999 - 2002年的群体结构比较时发现,群体结构趋于简单;对病原菌...  相似文献