Pseudomonas sp.20#-5防治烟草黑胫病田间试验初报   总被引：2，自引：0，他引：2       下载免费PDF全文

杨廷  张伏军  袁志辉  胡军华  肖杰  蓝希钳  周泽扬 《植物保护》2007,33(3):130-132

20^#-5菌株是本实验室从土壤中分离保存的1株假单胞菌,2006年对其发酵液防治烟草黑胫病进行了田间防效试验。结果显示,不同发病时期20^#-5菌发酵液的防治效果在64.39%～79.70%,高于对照药剂,防治效果较好。不同施药时期调查结果表明,发病前施用20^#-5菌发酵液的防治效果要好于发病后施用,说明20^#-5菌发酵液对烟草黑胫病具有有效的预防作用。  相似文献   

Genetic characterization by fluorescent AFLP of Pseudomonas savastanoi pv. savastanoi strains isolated from different host species     

A. Sisto  M. G. Cipriani  S. Tegli  M. Cerboneschi  G. Stea  E. Santilli 《Plant pathology》2007,56(3):366-372

The genetic diversity of 71 Pseudomonas savastanoi pv. savastanoi strains isolated from different host species and from diverse geographical regions was determined by fluorescent amplified fragment length polymorphism (f-AFLP) analysis. The study was carried out using three different selective primer combinations. Strains of P. syringae pv. syringae , P. syringae pv. phaseolicola , P. syringae pv. glycinea , P. syringae pv. tagetis and P. amygdali were also included as outgroups. Based on cluster analysis of f-AFLP data, all P. savastanoi pv. savastanoi strains showed a high degree of similarity, grouping in a cluster and forming a taxon clearly separate from outgroup strains. AFLP analyses failed to support placing strains of P. savastanoi pv. savastanoi , P. syringae pv. phaseolicola and P. syringae pv. glycinea in the same species. Strains of P. savastanoi pv. savastanoi formed subclusters that correlated with the host species. Strains identified within these subclusters were related to the geographical region where the strains were isolated. Strains of P. savastanoi pv. savastanoi from olive were divided into two subclusters. Strains from oleander were differentiated from those from ash and were divided into two additional subclusters, distinct from olive strains. Three strains isolated from jasmine showed a high level of similarity among them but, at a lower Dice similarity coefficient, were linked to a subcluster including olive strains. Finally, two strains isolated from privet were similar to strains from olive and were included in the same subcluster.  相似文献   

Phenotypic variability in different strains of Pseudomonas syringae subsp. savastanoi isolated from different hosts     

F. Alvarez  J.E. García de los Ríos  P. Jimenez  A. Rojas  P. Reche  M.T. Troya 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):603-609

One hundred and sixty strains of Pseudomonas syringae subsp. savastanoi from Olea europaea, Olea europaea var. sylvestris, Nerium oleander, Fraxinus angustifolia and Retama sphaerocarpa, and four type strains of other pathovars were studied, investigating 102 phenotypic traits, among which we include biochemical characteristics, assimilation of different carbon sources, sensitivity or resistance to antibiotics and indoleacetic acid (IAA) production. Results were analysed with an affinity dendrogram via the Jaccard coefficient. They indicate an influence of environmental factors on the formation of the 15 phenons obtained, since isolated (knot) strains from the same species but different geographical areas are segregated. Segregation, also detected in strains from different hosts within the same area, added to the pathogenicity test helps to characterise these strains as different pathovars.  相似文献   

Leaf necrosis and twig dieback of sweet persimmon (Dyospiros kaki L.) caused by Pseudomonas syringae pv. syringae in Italy     

M. Scortichini  D. D'Ascenzo  Daniela Di Silvestro 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(6):625-627

In spring 1996, extensive leaf necrosis and twig dieback were observed on young sweet persimmon (Dyospiros kaki L.) trees, cultivars O'Gosho, Hachija, Mercatelli and Kaki-tipo planted in the Abruzzo region (central Italy). Many trees were killed. When the dieback reached the trunk, in many cases, new vegetation was noticed above the graft point. The cultivar Jiro-C was not affected by the disease. During 1997, no symptoms were observed on any plant. The orchard was planted in a clay soil with a very low content of organic matter. Biochemical, nutritional and pathogenicity tests indicated Pseudomonas syringae pv. syringae van Hall as the causal agent of the disease. This is the first report of this bacterium as a pathogen of sweet persimmon in Europe.  相似文献   

木麻黄青枯菌的根表吸附及根内增殖与其致病性关系   总被引：3，自引：0，他引：3       下载免费PDF全文

罗焕亮  王军  邵志芳  张景宁 《林业科学研究》2002,15(1):13-20

对 3个不同致病的青枯菌菌株和 7个不同抗病性的木麻黄无性系苗的研究得出 ,各菌株对寄主根表吸附量具有显著差异 ,吸附量与致病性呈负相关 :各菌株在寄主根内的增殖量差别明显 ,增殖量与致病性呈正相关。病菌脂多糖 (LPS)对幼苗根的预处理 ,可使其供体菌的吸附量减少5 8.5 %　 97.3% ,但胞外多糖 (EPS)对供体菌的吸附量无明显影响。洗去EPS和LPS的菌体较完整菌体对根表的吸附量减少 6 3.3%　 74 .2 %。而仅洗去EPS的菌体对根表的吸附有所增加 ,最高达19.1%。各菌体SDS PAGE电泳图谱显示出一定差异 ,其中强毒菌株 6 0 1B具有Rf=0 .35的特异谱带。结果表明青枯菌对木麻黄的致病性与其在寄主根表的吸附情况和在根内的快速增殖具有密切关系。LPS在此病理系统中 ,起着细菌识别因子的作用 ,EPS对LPS进行掩盖 ,二者都是病菌致病性的重要因子  相似文献   

两种抗生细菌拌种飞播造林试验   总被引：1，自引：0，他引：1       下载免费PDF全文

周显明  余金勇  胡炳福  罗中康  黄以黔  刘明灿  李心悦  邬海鸥  杨世平  毛济华 《林业科学研究》1992,5(4):474-477

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杉木细菌性叶枯病的发生及防治措施     

郑金凤 《福建林业科技》2007,34(2):41-43,47

杉木细菌性叶枯病是杉木的主要病害之一,由丁香假单胞杆菌杉木致病变种(Pseudomonas syringae pv. cunning-hamiae)引起。本文综合阐述了其发病原因和发病规律,并针对其发病特点提出了综合防治措施。  相似文献   

检测水貂出血性肺炎绿脓杆菌的比色LAMP法的建立与应用     

韩明明  ;刘旭平  ;母连志  ;赵静  ;刘慧  ;刘晓飞 《兽医大学学报》2014,(4):583-587

为了快速有效检测水貂出血性肺炎病原绿脓杆菌,本研究结合金属指示剂HNB特性建立了快速检测绿脓杆菌比色LAMP法。根据绿脓杆菌外毒素A基因设计引物,建立了检测LAMP法;并且对该方法进行了特异性、灵敏性分析;同时进行了初步应用。结果显示,该方法特异性强,灵敏性好,可检测167CFU/mL的菌体,对病貂肺脏检出率高。结果表明,该比色LAMP法可以有效地检测水貂出血性肺炎绿脓杆菌。  相似文献   

白鲢出血病病原菌的鉴定   总被引：7，自引：0，他引：7  

何顺华  傅锦楠 《水产科技情报》1993,20(2):75-77

从患出血病的白鲢体内分离出两株细菌(编号为水_1、水_2),分别感染健康白鲢,人工复制白鲢出血病获得成功。对这两株细菌进行形态、营养需求、生长特性、以及生化反应等观察,作者认为,水_1菌为产碱假单胞菌(Pseudomonas alcaligenes);水_2菌为嗜水气单胞菌(Aeromonas hydrophila)。前者隶属于假单胞菌科(Pseudomonaceae),后者隶属于孤菌科(Vibrionaceae)。  相似文献   

Pseudomonas anguilliseptica infection in farmed cod, Gadus morhua L   总被引：1，自引：0，他引：1  

Ferguson HW  Collins RO  Moore M  Coles M  MacPhee DD 《Journal of fish diseases》2004,27(4):249-253

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