烟台威海沿海菲律宾蛤仔线粒体遗传多样性分析     

毕晓欣  黄玲  靖美东  韩立亚  郝月 《安徽农业科学》2012,40(2):673-674,677

[目的]了解我国沿海野生菲律宾蛤仔群体遗传多样性和遗传分化情况。[方法]采用PCR技术对烟台威海2个群体的线粒体COⅠ基因片段进行扩增和序列分析。[结果]50个样本均得到684 bp的COⅠ基因片段序列,共定义了42个单倍型,检测到62个变异位点,2个群体单倍型多样性都较高,而核苷酸多样性相对偏低。用最大似然法(Maximum Likelihood,ML)构建的单倍型系统进化树显示2个地理种群的样本呈现散在分布,无明显的种群分化。[结论]反映了海洋动物的基因交流较容易,不同海域隔离较弱。  相似文献   

人工林落叶松髓心方材的高频真空干燥(英文)     

李晓玲  高瑞清  构涛  张立宾  陈章景 《林业科学》2012,48(9):127-131

对截面尺寸为100mm×100mm的人工林落叶松髓心方材进行系列高频真空干燥试验,分析研究不同高频真空干燥条件下干燥质量和干燥速度的变化规律,获得合理的高频真空干燥工艺基准;为了进一步减少试材表裂,干燥前对试材进行切口和过热蒸汽预处理。结果表明:沿试材长度方向的中心位置切口和过热蒸汽预处理都能有效减少试材表裂,提高干燥质量。  相似文献   

Consumption of Bark and Seeds by Voles in Relation to Habitat and Landscape Structure     

《Scandinavian Journal of Forest Research》2012,27(1):28-34

Voles cause damage in forestry by eating the bark of seedlings and the seeds of conifers. Folivorous field voles ( Microtus agrestis ), restricted to various types of grassland, are mainly responsible for damage to bark, and granivorous bank voles ( Clethrionomys glareolus ), supported by most forest environments, for the consumption of seeds. Densities of bank and field voles, consumption of bark on indigenous and experimental woody plants, and consumption of experimentally supplied seeds were measured during the vole cycle 1997-2000 in relation to three habitat and three landscape variables. Landscape variables explained field vole densities and consumption of bark to a considerable extent, while habitat variables were more adequate for densities of bank vole and consumption of seeds. Field vole populations may demonstrate a ''mass effect'', where the success of early development and dispersal from subpopulations will decide peak numbers over entire landscapes. Numbers of field voles may affect numbers of the inferior bank vole. Thus, predicting the exact location of vole damage is principally difficult.  相似文献   

益康XP替代抗生素对早期断奶藏羔羊应激和免疫的影响     

李敏  吕凯  侯生珍  郭远玉  刘阳梅 《饲料工业》2012,33(21)

选取早期断奶([55±2)日龄,体重(7.91±0.83)kg]藏羔羊42只,随机分为3组(每组14只,公母各半),饲喂基础日粮为粗蛋白和能量水平分别为18.02%和12.95 MJ/kg开口料。试验Ⅰ组为基础日粮,试验Ⅱ组为基础日粮+1%益康XP,试验Ⅲ组为基础日粮+0.03%莫能菌素。对早期断奶藏羔羊血清中反应应激的酶类指标(Cor、CK、LDH、AST、ALP)和免疫指标(IgG、IgA、IgM)进行了测定。试验结果表明:各试验组的Cor、CK、AST和LDH差异不显著(P>0.05),但Cor、CK试验Ⅱ组小于其它两组,AST试验Ⅱ组小于试验Ⅲ组、LDH试验Ⅲ组小于其它两组;ALP试验Ⅱ组明显大于试验Ⅲ组(P>0.05);试验Ⅱ组IgG、IgA、IgM高于试验Ⅰ组和试验Ⅲ组(P>0.05);试验Ⅱ组藏羔羊的日增重明显高于试验Ⅰ组和试验Ⅲ组,差异显著(P<0.01);试验Ⅱ组藏羔羊的日采食量高于试验Ⅰ组和试验Ⅲ组,试验Ⅱ组对藏羔羊的腹泻率明显低于试验Ⅰ组和试验Ⅲ组(P<0.05)。综合评价表明基础日粮中添加益康XP能减小藏羔羊的早期断奶应激,提高早期断奶藏羔羊免疫机能,是抗生素的一种优良替代品。  相似文献   

Assessment of Genetic Diversity of Drought Tolerant and Susceptible Rice Genotypes Using Microsatellite Markers     

Ravindra DONDE  Jitendra KUMAR  Gayatri GOUDA  Manoj Kumar GUPTA  Mitadru MUKHERJEE  Sk Yasin BAKSH  Pradosh MAHADANI  Khirod Kumar SAHOO  Lambodar BEHERA  Sushanta Kumar DASH 《水稻科学》2019,26(4):239-247

The introgression of wild chromosomal segments into popular rice varieties is one of the potential approaches for developing varieties for drought stress condition. Sixteen genotypes, including nine indica, two tropical japonica and five chromosome segments substitution lines(CSSLs) with different levels of tolerance/susceptibility to drought stress, were selected for diversity study. Sixty-three microsatellite markers were utilized for assessing genetic diversity. A total of 95 alleles were amplified, and out of them, 60 were polymorphic. Six unique alleles, amplified by the microsatellite loci RM276, RM472,RM488, RM537, RM541 and RM28089, were identified in six genotypes, namely FR13A, Brahamanakhi,RUF44, Swarna-sub1, Brahamanakhi and Satyabhama. The highest genetic similarity was found among CSSLs. Polymorphism information content(PIC) value varied from 0 to 1.00 with an average of 0.66 per locus. Twenty-eight microsatellites were found to be polymorphic, which could be used in marker-assisted selection programme. All the sixteen genotypes were grouped into two major clusters at genetic similarity of 0.64. In the cluster I, five CSSLs identified as diverse genotypes had wild ancestor segments responsible for drought tolerance, and hence they could be utilized as potential donors. The popular Indian varieties, Swarna-sub1 and IR64-sub1, could be used as recurrent parents in the future breeding program for developing varieties for abiotic stresses such as submergence and drought.  相似文献   

Genetic variations and interrelationships in Vanilla planifolia and few related species as expressed by RAPD polymorphism     

D. Minoo  V. N. Jayakumar  S. S. Veena  J. Vimala  A. Basha  K. V. Saji  K. Nirmal Babu  K. V. Peter 《Genetic Resources and Crop Evolution》2008,55(3):459-470

Vanilla is naturally distributed in Mexico and parts of Central America and the history of origin of cultivated vanilla suggests that the entire stock outside Mexico may be from a single genetic source. In the present study, RAPD polymorphism was used to estimate the level of genetic diversity and interrelationships among different collections of Vanilla planifolia Andr., and few related species, including both leafy and leafless types such as V. tahitensis J.W.Moore, V. andamanica, Rolfe, V. pilifera Holtt., and V. aphylla Blume. Studies revealed that there are very limited variation within collections of V. planifolia, indicative of its narrow genetic base, and of the related species we tested, V. tahitensis is nearest to V. planifolia. The species studied are diverse and have a similarity ranging from 1.2 to 57.3 %. Of the sampled taxa, V. andamanica is the most divergent and there is also reasonable variability within its collections, indicating the possibility of natural seed set. A total of 82 polymorphic bands expressed in the RAPD profiles were used to generate a genetic distance matrix, which was then used in cluster analysis. Specific groupings were revealed by the cluster analysis whereby the leafless forms (V. aphylla, V. pilifera and the new species) and V. andamanica formed separate clusters. This is the first report of species interrelationship studies, including both cultivated and wild vanilla species.  相似文献   

Plant Species Composition and Forage Production 14 Yr After Biosolids Application and Grazing Exclusion     

E. Avery  M. Krzic  B.M. Wallace  R.F. Newman  G.E. Bradfield  S.M. Smukler 《Strength and Conditioning Journal》2019,72(6):996-1004

This paper examines the effects of a single surface application of biosolids (at 20 dry Mg ha^{? 1}) on plant species composition, forage quality and quantity, and C stocks after 14 yr of rest in rangelands of the Central Interior of British Columbia. More than two times the aboveground biomass of grasses and the percent cover of plant litter were found in the biosolids treatment relative to the control, along with reductions in bare soil and microbiotic crust cover. Significantly greater plant uptake of all macronutrients (C, N, P, K, S, Ca, and Mg); most micronutrients (B, Cu, Mn, Mo, and Zn); and Al occurred in the biosolids treatment. P and Cu were the only two nutrients to be more concentrated in the biosolids-treated forage relative to the control forage, while N, Mg, and protein were more concentrated in the control forage. No significant difference in forage digestibility was found between biosolids and control treatments. Bluebunch wheatgrass, the late-seral native grass species, had significantly increased cover and aboveground biomass in the biosolids treatment relative to the control; however, between 2006 and 2016, non-native Kentucky bluegrass had reached > 25% cover in the biosolids plots, perhaps restricting the full recovery of bluebunch wheatgrass. Our findings indicate that biosolids application to ungrazed rangeland can increase long-term forage production and reduce bare soil. However, at our study site biosolids application also led to a long-term shift in the plant community composition away from the late-seral (i.e., bluebunch wheatgrass) trajectory, and the effects of this shift on rangeland health and productivity require further investigation.  相似文献   

ASSESSMENT OF INTRAOCULAR PRESSURE BY APPLANATION AND REBOUND TONOMETRY IN GUINEA PIGS OF DIFFERENT AGES     

Martí Cairó  María Teresa Peña  José Rios  Adrià Melero  Jaume Martorell  Marta Leiva 《Journal of Exotic Pet Medicine》2018,27(1):25-31

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Interspecific hybridization between Vigna unguiculata (L.) Walp. and V. vexillata (L.) A. Rich. through in vitro embryo culture     

P. Gomathinayagam  S. Ganesh ram  R. Rathnaswamy  N.M. Ramaswamy 《Euphytica》1998,103(2):203-209

Immature embryos resulting from the cross V. vexillata × V. unguiculata were cultured on MS medium supplemented with 2,4-D (2 mg/l) and resulted in embryogenic calli. Thirteen hybrid regenerants were obtained via organogenesis by subculturing the calli on MS medium supplemented with BAP (2 mg/l) + adenine sulphate (40 mg/l) + CH (500 mg/l) + cowpea tender pod extract (10%). The interspecific regenerants showed intermediate morphological traits between the parents for leaf shape, pod colour and seed coat colour. The hybrid plants inherited stem, leaf and pod hairiness of the wild species which could serve as a mechanical barrier against viral vectors. Electrophoretic studies of two isozyme systems, peroxidase and esterase, also confirms the hybrid nature of the regenerants as they expressed unique bands of both parents. Cytological study of the meiotic chromosomes revealed high frequency of univalent formation in the hybrids suggesting that the genomes of the parental species are structurally differentiated. The hybrid regenerants exhibited high enzyme activity for three enzymes viz., peroxidase, polyphenol oxidase and phenyl alanine ammonia lyase over the cultivated parent which may be useful in conferring resistance against viral pathogens. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

猪繁殖与呼吸综合征病毒实验感染SPF猪外周血单核细胞和肺泡巨噬细胞早期凋亡细胞的观察   总被引：7，自引：0，他引：7  

张晓梅  杨汉春  李华  任慧英  高齐瑜  陈艳红 《中国兽医杂志》1999,25(11):11-13

采用Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ／ＰＩ双染色法,用流式细胞仪检测了猪繁殖与呼吸综合征病毒（ＰＲＲＳＶ）实验感染ＳＰＦ猪不同时期外周血单核细胞和肺泡巨噬细胞感染Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群（早期凋亡细胞群）。结果显示,ＰＲＲＳＶ感染猪外周血单核细胞和肺泡巨噬细胞Ａｎｎｅｘｉｎ Ｖ－ＦＩＴＣ＾＋／ＰＩ＾－细胞群的表达率均明显高于正常对照猪,感染后２４ｈ表达率达最高值。  相似文献