水稻香豆酸辅酶A连接酶基因的分子克隆及一级结构分析   总被引：2，自引：1，他引：2  

沈桂芳  赵衍 《中国农业科学》1991,24(4):1-7

 香豆酸：辅酶A连接酶（4CL）是类黄酮生物合成过程中的关键酶之一。本文以水稻为材料分离、克隆了4CL基因，并测定了该基因的一级结构。水稻4CL基因含有5个外显子，共编码563个氨基酸。外显子/内含子交界顺序和多数已报道的其他植物基因相似，边界剪切位点符合GT-AG规则。编码区中同义密码有明显的偏态使用现象，导致整个编码顺序GC含量升高。在非编码区，除了有5'端真核生物基因启动转录所必需的TATA盒以及3'-端mRNA多A加尾信号之外，在TATA盒的上游有一个在碱基顺序和位置上都十分类似于“光盒”的结构。  相似文献   

氮肥水平对杂交稻汕优63剑叶光合速率和RuBP羧化酶活性的影响   总被引：18，自引：0，他引：18  

王仁雷  李霞  陈国祥  华春  魏锦城 《作物学报》2001,27(6):930-934

用低氮(LN)、中氮(MN)、高氮(HN)盆栽土培的杂交水稻汕优63为材料,测定其剑叶的光合速率、RuBP羧化酶含量及活性、可溶性蛋白和叶绿素含量的变化.结果表明:随着氮水平的提高,叶面积增大,气孔密度减少,RuBP羧化酶活性和光合速率增加.随着叶龄的增加,三个不同氮水平的叶片中RuBP羧化酶蛋白下降均先于可溶性蛋白的下降;HN组的光  相似文献   

大蒜提取物对B16F10细胞内黑色素生成的抑制作用     

姚欣  曾洁琳  沈衡平  刘朕  范丽霞  杨安平  刘辉 《湖南农业大学学报(自然科学版)》2022,48(6)

采用MTT法测定大蒜提取物、二胜肽和六胜肽3种物质对B16F10细胞活力的影响,且在非毒剂量下,分别评价此3种物质对B16F10细胞黑色素生成和细胞形态的影响;在单因素试验基础上,采用正交试验设计大蒜提取物、二胜肽和六胜肽组合物中各组分的用量,检测组合物对B16F10细胞黑色素生成抑制率的影响,并利用Western blot检测最佳组合物对黑色素合成相关蛋白TYR、TRP–1和TRP–2表达的影响。结果表明：0.00~2.50 μg/mL的大蒜提取物、0.00~4.00 μg/mL的二胜肽或六胜肽对B16F10细胞的细胞活力和细胞形态均没有显著影响;大蒜提取物在0.00~2.50 μg/mL、二胜肽和六胜肽在0.00~4.00 μg/mL内呈剂量依赖性的抑制黑色素的生成;当大蒜提取物、二胜肽、六胜肽质量浓度分别为2.50、1.00、0.50 μg/mL时的组合物抑制黑色素的生成作用效果最佳;该最佳组合物对黑色素合成相关蛋白TYR、TRP–1和TRP–2的表达具有明显的抑制作用,表明组合物抑制黑色素的生成与TYR、TRP–1和TRP–2蛋白水平的降低有关。  相似文献   

普安银鲫成熟卵及受精后FAS和ACC的表达     

姚俊杰  朱俊华  朱忠胜  田超  王秀龙 《贵州农业科学》2015,(2):107-109,114

为探明普安银鲫雌鱼不同发育时期与脂代谢相关的脂肪酸合成酶(FAS)、乙酰辅酶A羧化酶(ACC)浓度及相对表达量的变化,以了解胚胎早期在脂质合成代谢方面的特点和规律性,采用酶联免疫分析法和实时荧光定量PCR法,测定成熟卵、受精卵、囊胚期(卵裂期胚胎)的FAS和ACC代谢水平及基因表达情况。结果表明:普安银鲫雌鱼成熟卵、受精卵和囊胚期均存在FAS和ACC,FAS含量在囊胚期显著升高(P0.05),达到(2.48±0.31)nmol/L;ACC含量上升但差异不显著(P0.05)。普安银鲫成熟卵、受精卵和囊胚期中均存在FAS和ACC基因表达,相对表达量逐渐增高,但差异均不显著(P0.05)。  相似文献   

黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响     

郭芳  谭峥  刘兴旺  刘丽英  任华中 《华北农学报》2011,(5):21-24

为了解黄瓜中ppc基因在各组织的转录水平及不同光强对叶片中该家族基因转录水平的影响,本研究对已克隆得到的Cs-ppc1、Cs-ppc2和Cs -ppc3基因的转录水平进行荧光定量PCR分析.Cs-ppc1和Cs-ppc3在多个组织中的表达量均较高,Cs-ppc2基因的组织表达差异性较大,花中的表达量高而在其他组织中的表...  相似文献   

Metabolomics - A robust bioanalytical approach for the discovery of the modes-of-action of pesticides: A review   总被引：1，自引：0，他引：1  

Konstantinos A. Aliferis  Suha Jabaji 《Pesticide biochemistry and physiology》2011,100(2):105-117

The agrochemical industry is facing great undertaking that includes increasing demand for the development of new crop protection agents that are safe for the environment and the consumers, and at the same time combat the issue of the emergence of resistance pest strains. The mode-of-action (MoA) is among the features of a bioactive compound that largely determine whether the abovementioned issues are addressed or not, and subsequently whether its commercial development will be addressed. The early discovery of the MoA of bioactive compounds could accelerate pesticide research and development by reducing the required time and costs. Based on advances in synthetic and natural product chemistry, scientists have access to a vast number of compounds that could potentially be developed as crop protection agents. The screening of such compounds with respect to their MoA requires accurate and robust bioanalytical tools. Metabolomics is a powerful bioanalytical tool that will likely play a significant role in the acceleration of the discovery of MoA of bioactive compounds. In the present review, the capabilities and principles and applications of metabolomics for the study of the MoA of herbicides, insecticides, acaricides, fungicides, and antibiotics are discussed.  相似文献   

大肠杆菌乙酰辅酶A羧化酶accD亚基表达载体的构建及遗传转化研究     

王伏林  郎春秀  刘仁虎  吴关庭  冯都华  陈锦清  石春海 《核农学报》2011,25(6):1129-1134

为探索大肠杆菌异质型乙酰辅酶A羧化酶（ACCase）的β-CT亚基accD基因对油料作物含油量的影响,将目的基因E.coli accD（eaccD）与拟南芥Rubisco小亚基（rbcS）转运肽基因融合,构建以油菜Napin启动子驱动的种子特异表达载体。本试验应用农杆菌介导法,将该表达载体转入油菜,通过PCR检测获得了...  相似文献   

Relationship between adipose maturity and fatty acid composition in various adipose tissues of Japanese Black,Holstein and Crossbred (F1) steers     

Ju‐Hwan LEE  Ichiro YAMAMOTO  Jin‐Suk JEONG  Toshihiro NADE  Toshiro ARAI  Nobuhiro KIMURA 《Animal Science Journal》2011,82(5):689-697

The amount of monounsaturated fatty acid (MUFA) is intimately related to adipose softness, melting point (MP) and flavor in beef. Stearoyl‐CoA desaturase (SCD) is a main gene involved in MUFA synthesis. Mature adipose tends to be highly saturated, whereas immature or maturing adipose is highly unsaturated when chronologically based, so the degree of non‐saturation can be an index of adipose maturity. In this study, three different adipose tissues (coelomic (CL), perirenal (PR), and subcutaneous (SC)) from three beef breeds with differing slaughter ages (Japanese Black (29.5 months), Holstein (20.1 month), and F1 crossbreed (25.6 months)) were examined to: (i) determine adipose maturity level as indexed by MUFA %; and (ii) determine SCD and other lipogenic gene messenger RNA (mRNA) expression levels in relation to unsaturated fatty acid content. Fatty acid composition was significantly different between adipose tissues (P < 0.05). MUFA amount was high in the following order: SC > CL > PR. This pattern corresponded to SCD mRNA expression profile showing higher expression in SC than CL and PR. However, Japanese black cattle are an exception with CL adipose containing similar UFA % as SC adipose, yet having the lowest SCD mRNA expression level among all adipose tissues tested. Therefore, SCD mRNA expression and MUFA % appear to be directly related; however, differences in SCD mRNA expression among three adipose tissues may reflect differences in the fat development characteristics affected by chronological age of the cattle breeds.  相似文献   

乙酰乙酸乙酯从盐酸介质中萃取铁的研究   总被引：1，自引：0，他引：1  

周革菲  张曼平  温传河 《青岛农业大学学报(自然科学版)》2000,17(2):128-131

用萃取法从盐酸溶液中分离Fe(Ⅲ ) ,采用常用的有机中间体乙酰乙酸乙酯为萃取剂 ,研究酸度、温度以及盐析剂等条件对萃取分配系数的影响 ,并对铁的反萃取进行讨论 ,从而确定最优萃取条件 ,为植物体内铁的萃取富集分离提供实验依据。  相似文献   

Seasonal Patterns of Photosynthetic Traits in Early Maturing Maize     

P. Stamp 《Journal of Agronomy and Crop Science》1988,160(3):183-190

A biochemical approach to maize breeding must be based on an exact knowledge about changes of relevant traits with ontogenetic stage. In 1983 seasonal patterns of net assimilation rate (NAR), chlorophyll and carotenoids contents, and of activities of RuBP carboxylase, PEP carboxylase and NADP malate dehydrogenase were recorded for two early maturing maize cultivars. For all traits the general trends were similar for both cultivars throughout the growing season but short term fluctuations were less synchronized after anthesis. About 75% of the maximum green leaf area was expanded within 20 days around middle of July. Specific seasonal patterns were observed for all traits. Before anthesis values were maximum for NAR in late June and in early July, for enzyme activities at the end of June and for pigment contents at the beginning of July. First decreases occurred for all traits before anthesis, further decreases were observed for PEP carboxylase in August and for chlorophyll content in September. Marked temporary declines coincided for NAR and pigment contents with a cold spell end of June and for these traits and RuBP carboxylase activity with anthesis. Evidence was scarce that any of the photosynthetic traits reflected the photosynthetic efficience of the two cultivars for a long stretch of time.  相似文献