猪链球菌病的诊断与治疗   总被引：1，自引：0，他引：1  

刘俊伟  司红英  韩天河  乔盼盼  王天有 《贵州农业科学》2009,37(1)

采用临床诊断学、病理解剖学和微生物学等技术,对临床上送诊病猪进行了全面、详细的检查.结果表明:病猪体温升高达41～42℃.耳廓、胸、腹下及四肢内侧皮肤呈紫红色,并有大量出血斑.跗关节肿大,关节囊内有黄色胶样液体渗出物.全身淋巴结肿大、出血,呈紫红色.肺充血肿胀,散在分布有大面积的肝变区.肾脏色泽较淡,表面有大量出血点和出血斑.肝、脾肿大,色泽较暗,呈黑褐色.脑膜充血、出血.在血液涂片及肝、脾和淋巴结的触印片上均见有大量链球菌.从而排除了强致病性蓝耳病,确诊为猪链球菌病.药敏试验结果表明,萘夫西林钠、苯唑西林钠为敏感,磺胺间甲氧嘧啶钠为中敏,其他抗菌药物均耐药.并为猪链球菌病正确、有效的治疗提供了科学依据.  相似文献   

Use of terminal restriction fragment length polymorphism (T-RFLP) for identification of phytoplasmas in plants   总被引：1，自引：0，他引：1  

J. Hodgetts  T. Ball  N. Boonham  R. Mumford  M. Dickinson 《Plant pathology》2007,56(3):357-365

A terminal restriction fragment analysis (T-RFLP) technique was developed for the simple and rapid detection and diagnosis of phytoplasmas in plants. The selected primers amplified part of the 23S rRNA gene to provide improved resolution between the taxonomic groups compared to conventional restriction enzyme analysis of the 16S rRNA. Using the restriction enzymes Bsh 12361 and Mse I on the PCR products, and fragment analysis in the range 68–640 bp, the technique was tested on 37 isolates from 10 of the 16Sr groups. Distinct and unambiguous T-RFLP profiles were produced for nine of the 10 taxonomic groups, such that almost all isolates within a group shared the same profile and could be distinguished from isolates in other groups. The technique also identified the presence of mixtures of phytoplasmas from different groups in samples. Furthermore, the primers were devised to amplify a terminal restriction fragment (TRF) product of a specific defined size (461 bp) from the host plant chloroplast DNA, so that there was a built-in internal control in the procedure to show that the absence of a phytoplasma peak in a sample was the result of no detectable phytoplasma being present, not the result of PCR inhibition. This method offers the possibility of simultaneously detecting and providing a taxonomic grouping for phytoplasmas in test samples using a single PCR reaction.  相似文献   

Development of a semiselective medium for isolating Xanthomonas campestris pv. musacearum from insect vectors, infected plant material and soil     

M. Mwangi  M. Mwebaze  R. Bandyopadhyay  V. Aritua  S. Eden-Green  W. Tushemereirwe  J. Smith 《Plant pathology》2007,56(3):383-390

A semiselective medium was developed for isolating Xanthomonas campestris pv. musacearum ( Xcm ) from infected banana plants, soil and insect vectors. The new medium was named cellobiose-cephalexin agar (CCA) and it contained (L⁻¹): 1 g yeast extract, 1 g glucose, 1 g peptone, 1 g NH₄Cl, 1 g MgSO₄·7H₂O, 3 g K₂HPO₄, 1 g beef extract, 10 g cellobiose, 14 g agar, 40 mg cephalexin, 10 mg 5-fluorouracil and 120 mg cycloheximide. The medium was evaluated for selectivity using 21 bacterial isolates and for plating efficiency using Xcm . The bacterial isolates included a soilborne Xanthomonas species and three pathogenic Xanthomonas strains that infect cassava, cabbage and beans. Although the plating efficiency of Xcm on CCA was lower (59%) than on non-selective yeast extract peptone glucose agar (YPGA), its selectivity was significantly higher, averaging 60 and 82%, when isolating from banana fruits and soil, respectively. CCA was also superior when isolating Xcm from insect vectors, with selectivity of 48–75%, compared with 8–17% on YPGA. Xanthomonas campestris pv. phaseoli did not grow on CCA, while X. campestris pv. campestris and X. axonopodis pv. manihotis grew, but their colonies were smaller than those of Xcm . Twenty-nine out of 33 suspected Xcm strains isolated from plants, soil and insects using CCA were pathogenic when inoculated onto banana plants, indicating that CCA can be a reliable tool in isolating Xcm populations. The medium should prove useful in studies on ecology, epidemiology and management of the banana bacterial wilt pathogen that is currently ravaging bananas in East and Central Africa.  相似文献   

Co-operational PCR coupled with dot blot hybridization for detection and 16SrX grouping of phytoplasmas   总被引：1，自引：0，他引：1  

E. Bertolini  E. Torres  A. Olmos  M. P. Martín  A. Bertaccini  M. Cambra 《Plant pathology》2007,56(4):677-682

A protocol based on Co-operational PCR has been successfully applied to the detection of phytoplasmas. A triprimer reaction coupled with hybridization using general and specific probes permitted detection of ' Candidatus Phytoplasma mali', ' Ca . Phytoplasma prunorum' and ' Ca . Phytoplasma pyri', and their identification as members of 16S ribosomal quarantine group X. The sensitivity of this method was at least one hundred times greater than conventional PCR and similar to that achieved by nested PCR and real-time PCR. The method was validated by testing field samples collected from Malus , Prunus and Pyrus spp. and Olea europaea and compared with seven phytoplasmas maintained in Catharanthus roseus .  相似文献   

Characterisation of <Emphasis Type="Italic">Phoma tracheiphila</Emphasis> by RAPD-PCR,microsatellite-primed PCR and ITS rDNA sequencing and development of specific primers for <Emphasis Type="Italic">in planta</Emphasis> PCR detection     

Virgilio?Balmas  Barbara?Scherm  Stefano?Ghignone  Ali?Ould?Mohamed?Salem  Santa?Olga?Cacciola  Quirico?MigheliEmail author 《European journal of plant pathology / European Foundation for Plant Pathology》2005,111(3):235-247

Thirty six isolates of Phoma tracheiphila from Italy, the causal agent of the mal secco disease on Citrus species, were characterised by different molecular tools in comparison with representative isolates of other phytopathogenic Phoma species. These included analysis of the distribution of RAPD and microsatellite markers and sequencing of the internal transcribed spacer (ITS) region of the nuclear rRNA genes. The results obtained with 12 RAPD primers (92 markers) and 7 microsatellite primers (56 markers) suggest that Italian isolates of P. tracheiphila are genetically homogeneous, leading to identical patterns upon amplification with all the tested primers. Accordingly, ITSI-5.8S-ITS2 sequences were highly conserved (98–100% identity along a 544-characters alignment) among all the isolates of P. tracheiphila. A neighbor-joining analysis of ITS sequences of P. tracheiphila in comparison with those of other Phoma species, as well as with alignable sequences from anamorphic and teleomorphic taxa retrieved in BLAST searches, revealed a close relationship between P. tracheiphila and Leptosphaeria congesta. A pair of P. tracheiphila-specific primers was designed on the consensus sequence (555 residues) obtained from the alignment of the newly generated P. tracheiphila ITS sequences. A PCR-based specific assay coupled to electrophoretic separation of amplicons made it possible to detect P. tracheiphila in naturally infected Citrus wood tissue collected from both symptomatic and symptomless plants. The limit of detection was 10 pg of genomic DNA and 5 fg of the ITS target sequence.  相似文献   

Reliable detection of the fungal pathogenfusarium oxysporum f.sp.albedinis, causal agent of bayoud disease of date palm, using molecular techniques     

Stanley Freeman  Marcel Maymon 《Phytoparasitica》2000,28(4):341-348

Bayoud, caused by the soilborne fungusFusarium oxysporum f.sp.albedinis (FOA), is the most serious disease of date palm. Since the disease is located in the North African countries of Morocco and Algeria, and advancing steadily eastwards, the ultimate goal is to prevent spread of the pathogen to other date-growing areas in the region and farther afield. Molecular diagnostic techniques have been developed for detection of FOA. In view of the fact that the fungus does not exist in Israel, DNA of FOA was obtained to determine the reliability of these methods for diagnostic purposes. Random amplified polymorphic DNA was not reliable enough for differentiation between FOA and various pathogenic and saprophyticFusarium isolates. However, the polymerase chain reaction utilizing FOA-specific primers was accurate and enabled amplification of a unique band specific to FOA DNA alone, and not that of the other tested pathogenic and saprophyticFusaria. The availability of a rapid and reliable diagnostic tool for detection of FOA will enable the Plant Protection and Inspection Services of the Israel Ministry of Agriculture to test date palm tissue for the presence of the pathogen. Contribution no. 513/00 from the Inst. of Plant Protection, ARO, The Volcani Center, Bet Dagan, Israel.  相似文献   

Lack of association between p53 SNP and  FISS in a cat population from Germany     

D. Mucha  S. Laberke  S. Meyer  J. Hirschberger 《Veterinary and comparative oncology》2014,12(2):130-137

One recent study indicates a significant association between certain single nucleotide polymorphisms (SNPs) in the genomic sequence of feline p53 and feline injection‐site sarcoma (FISS). The aim of this study was to investigate the correlation between a specific nucleotide insertion in p53 gene and FISS in a German cat population. Blood samples from 150 German cats were allocated to a control group consisting of 100 healthy cats and a FISS‐group consisting of 50 cats with FISS. All blood samples were examined for the presence of the SNP in the p53 gene. Results found the T‐insertion at SNP 3 in 20.0% of the cats in the FISS‐group and 19.2% of cats in the control‐group. No statistically significant difference was observed in allelic distribution between the two groups. Further investigations are necessary to determine the association of SNPs in the feline p53 gene and the occurrence of FISS.  相似文献   

Diagnostic accuracy of the SNAP and Spec canine pancreatic lipase tests for pancreatitis in dogs presenting with clinical signs of acute abdominal disease     

Mark D. Haworth BVSc  Giselle Hosgood BVSc  MS  PhD  DACVS  Katrin L. Swindells BVSc  DACVECC  Caroline S. Mansfield BSc  BVMS  PhD  DECVIM 《Journal of Veterinary Emergency and Critical Care》2014,24(2):135-143

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Diagnostic Methods for Detecting Internal Parasites of Livestock     

《Veterinary Clinics of North America: Food Animal Practice》2020,36(1):125-143

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Ditylenchus gigas n. sp. parasitizing broad bean: a new stem nematode singled out from the Ditylenchus dipsaci species complex using a polyphasic approach with molecular phylogeny     

N. Vovlas  A. Troccoli  J. E. Palomares‐Rius  F. De Luca  G. Liébanas  B. B. Landa  S. A. Subbotin  P. Castillo 《Plant pathology》2011,60(4):762-775

Morphologial, biochemical, molecular and karyological analyses of different populations and races of the stem and bulb nematode Ditylenchus dipsaci have suggested that it represents a species complex, of which only D. dipsaci sensu stricto and its morphologically larger variant, known as the giant race of the stem and bulb nematode, are plant parasites of economic importance. The present study singles out the giant race from this complex, herein described as a new species named Ditylenchus gigas n. sp., on the basis of morphological and molecular data obtained from several populations collected from broad beans in southern Italy, southern Spain and Lebanon. The new species epithet, which refers to the large body size of the nematode with respect to the normal races, must be considered to be conspecific with the D. dipsaci‘giant race’ from Fabaceae in recent literature. Morphologically, the new species is characterized by a body size 1·5–2 times longer than the ‘normal race’, stylet delicate (11·5–13·0 μm long) with knobs distinctly sloping backwards, and long post‐vulval uterine sac (81–150 μm long). Results of molecular analysis of rDNA sequences including the ITS1‐5.8S‐ITS2 region, the D2–D3 fragment of the 28S gene, the small 18S subunit, the partial mitochondrial gene for cytochrome c oxidase I (mtCOI), and hsp90 gene sequences, support the new taxonomic species status for the former D. dipsaci giant race from Vicia faba, and clearly distinguish D. gigas n. sp. from D. dipsaci sensu stricto.  相似文献