Wnt signaling pathway is involved in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells     

FU Shu-ping  YANG Li  HONG Hao  OU Chen  ZHANG Rong-hua 《园艺学报》2014,30(9):1656-1660

AIM：To investigate the changes of Wnt signaling pathway in catalpol-induced proliferation of rat bone marrow mesenchymal stem cells (BMSCs). METHODS：The BMSCs were isolated from SD rats, purified by differential time adherent method and divided into control group and catalpol (1.0 mg/L) group. Flow cytometry was used to detect the proliferation index of BMSCs. The mRNA levels of Wnt3a, Wnt5a, Wnt11 and β-catenin was evaluated by real-time PCR. In addition, the protein expression level of β-catenin was determined by Western blotting. RESULTS：Prolife-ration index was increased from 8.90%±0.46% to 17.93%±1.68% after treatment with catalpol (P<0.01). Compared with control group, the mRNA expression of Wnt5a, Wnt11 and β-catenin was all increased with catalpol treatment. No difference of Wnt3a mRNA expression between control group and catalpol group was observed. Meanwhile, the protein expression of β-catenin was increased in catalpol group compared with control group. CONCLUSION：Catalpol promotes BMSCs going into the cell cycle. Classical and non-classical Wnt signaling pathways are activated in this process.  相似文献   

Regulation of Tenomodulin Expression Via Wnt/β-catenin Signaling in Equine Bone Marrow-derived Mesenchymal Stem Cells     

Shihori MIYABARA  Yohei YUDA  Yoshinori KASASHIMA  Atsutoshi KUWANO  Katsuhiko ARAI 《Journal of Equine Science》2014,25(1):7-13

  相似文献   

Calcium Signaling is Involved in Negative Phototropism ofRice Seminal Roots简     

CHEN Juan  岛 Yi-wei  XU Hua-wei 《水稻科学》2014,21(1):39-46

Calcium ions(Ca2+) act as an intracellular second messenger and affect nearly all aspects of cellular life. They are functioned by interacting with polar auxin transport, and the negative phototropism of plant roots is caused by the transport of auxin from the irradiated side to the shaded side of the roots. To clarify the role of calcium signaling in the modulation of rice root negative phototropism, as well as the relationship between polar auxin transport and calcium signaling, calcium signaling reagents were used to treat rice seminal roots which were cultivated in hydroculture and unilaterally illuminated at an intensity of 100–200 μmol/(m2·s) for 24 h. Negative phototropism curvature and growth rate of rice roots were both promoted by exogenous CaCl2 lower than 100 μmol/L, but inhibited by calcium channel blockers(verapamil and LaCl3), calcineurin inhibitor(chlorpromazine, CPZ), and polar auxin transport inhibitor(N-1-naphthylphthalamic acid, NPA). Roots stopped growing and negative phototropism disappeared when the concentrations increased to 100 μmol/L verapamil, 12.500 μmol/L LaCl3, 60 μmol/L CPZ, and 6 μmol/L NPA. Moreover, 100 μmol/L CaCl2 could relieve the inhibition of LaCl3, verapamil and NPA. The enhanced negative phototropism curvature was caused by the transportation of more auxin from the irradiated side to the shaded side in the presence of exogenous Ca2+. Calcium signaling plays a key role as a second messenger in the process of light signal regulation of rice root growth and negative phototropism.  相似文献   

外源乙烯处理对黄冠梨果皮褐变和货架期品质的影响     

秦畅  李丹  赵韩栋  陈庆敏  傅茂润 《农业工程学报》2022,38(6):312-318

为了避免黄冠梨果实在低温贮藏（0 ℃左右）条件下果皮褐变的发生,该研究采用5、20和100 μL/L外源乙烯对黄冠梨进行贮前处理,以0 ℃贮藏下无外源乙烯处理作为对照组,以采后恒温至30 ℃、逐步降温至0 ℃处理作为程序降温组。通过外源乙烯处理组与对照及程序降温组的对比,探讨采后外源乙烯处理对黄冠梨果皮褐变与果实品质的影响。结果表明,不同浓度的外源乙烯处理均能完全抑制褐变的发生,且表皮无伤害,而程序降温组的发病指数为10%,对照组则高达37.6%。货架期结束时（6 d）外源乙烯处理组的果实硬度与对照组差异显著（P < 0.05）。外源乙烯各处理组果实含水率均高于对照及程序降温组（P < 0.05）,利用低场核磁共振技术检测发现对照组自由水含量最高,5和100 μL/L外源乙烯处理组自由水含量较低。与对照组和程序降温组比较,外源乙烯处理对黄冠梨果实中可溶性固形物、可滴定酸、可溶性糖和抗坏血酸等果实品质指标都能起到提高或者维持的效果,其中5 μL/L处理浓度的效果较优。研究结果表明,贮前外源乙烯处理是一种既能抑制黄冠梨果皮褐变又能保持果实货架期品质的高效、简便的技术。  相似文献   

动物体型大小调控分子机制研究进展   总被引：1，自引：0，他引：1  

任红艳 《湖北农业科学》2014,53(20):4783-4787

体型大小的差异是不同动物之间最明显的表型差异,了解动物体型大小的调控机制对于揭示生命的奥秘和保障人类的生命健康安全都具有重要的意义.随着分子生物学、发育生物学和功能基因组学等技术的发展,科学家们以果蝇、线虫等模式生物为研究材料,对调控细胞、器官和个体尺寸大小的机理进行了大量研究,结果发现,除了环境、营养等外在因素对体型大小的影响外,内在的微观因素(包括细胞大小与数量、DNA含量、激素水平、生长相关信号通路、特定基因等)起到了决定性作用,这些因素通过调控细胞数量与大小进而影响器官乃至个体的大小.对影响动物体型大小的基因及其信号通路进行了综述,目的是为动物体型大小调控机制研究提供理论参考.  相似文献   

昆虫滞育机制研究进展   总被引：1，自引：0，他引：1  

梁瀚清  钟杨生  陈芳艳  严会超  林健荣 《广东农业科学》2014,41(20):84-90

滞育是绝大多数昆虫为了能够在周期性的不利于其生存的自然环境中生存下来而进行的一种生理过程,它在昆虫的生存、进化和繁殖等方面发挥着重要的作用.主要从昆虫滞育发生的原因、影响昆虫滞育的内外因素、内分泌机制、滞育相关酶类、滞育的蛋白质组学和分子生物学、以及有关调控通路研究等方面介绍其研究进展,为今后从种群到个体,从宏观到微观,从激素、酶、关联蛋白三者之间的关系等角度研究和了解昆虫的滞育行为和滞育发生的分子机制提供参考.  相似文献   

Regulation of C/N Interaction in Model Plant Species     

《Journal of Crop Improvement》2013,27(2):127-173

Summary

Carbon (C) and nitrogen (N) metabolisms are two major metabolic pathways which have been intensively studied in plants. Both N and C metabolisms are tightly linked in numerous plant biochemical pathways. Therefore the C to N ratio has to be carefully regulated to ensure proper functioning of the huge metabolic network. In order to maintain a viable C/N status under a large range of growth conditions plants have evolved complex mechanisms to regulate the delicate network of these two major assimilatory pathways. C and N metabolisms are both highly regulated. We will present the current knowledge on the regulation of N and C metabolisms by sugars and N metabolites. Players involved in these regulatory processes are just starting to be uncovered and possible signaling molecules involved in these regulations as well as known or potential candidate regulatory genes willbe discussed.  相似文献   

水分胁迫下植物乙烯,脯氨酸积累气孔反应的研究现状   总被引：18，自引：1，他引：17  

辛国荣  董美玲 《草业科学》1997,14(1):62-66

对植物在水分胁迫下乙烯，脯氨酸积累，气孔反应的研究现状以及这些指标在水胁迫下的生理特征进行了探讨，以期为植物的抗旱性品种选育，提高作物产量，发展干旱地区农业生产提供参考。  相似文献   

包裹姜根油的多种纳米颗粒的制备及质量评价     

李惠敏  郑明彬  王柯敏  何晓晓  吴萍  谭蔚泓 《湖南农业大学学报(自然科学版)》2006,33(3)

以大豆磷脂、聚乙交酯丙交酯(PLGA)和聚乙二醇(PEG)3种原料成功制备了包裹难溶于水的植物提取物姜根油(GRO)的纳米脂质体、PLGA纳米颗粒和PEG纳米颗粒,并对3种不同载体制备的纳米制剂的形貌、大小、Zeta电位,包封率及物理稳定性等进行了考察.研究结果表明3种纳米颗粒各有优缺点,可以根据不同的条件和不同的需要制备不同的颗粒,为开发新型的难溶药用植物提取物的理想药剂提供了实验依据.  相似文献   

Effect of gonadotropin-releasing hormone analogue on human breast cancer cell line in vitro     

ZHANG Xiao-juan  DONG Shi-zhong  MA Yan-qing  YANG Lu-ming 《园艺学报》2015,31(1):130-134

AIM: To investigate the effects of gonadotropin-releasing hormone (GnRH) analogue on the growth of breast cancer cell lines MCF-7 and MDA-MB-231 in vitro and to explore the related mechanisms with PI3K/Akt or ERK/MAPK pathways. METHODS: The proliferation of human breast cancer cell lines MCF-7 and MDA-MB-231 treatment with triptorelin was detected by MTT assay and the distribution of the cell cycle was determined by flow cytometry. The phosphorylation of the ERK1/2 and Akt was evaluated by Western blotting. RESULTS: Triptorelin inhibited the proliferation of MCF-7 cells at concentration of 10^-5 mol/L after treated for 192 h or at concentration of 10^-4 mol/L after treated for 168 h and 192 h. Triptorelin inhibited the proliferation of MDA-MB-231 cells at concentration of 10^-4 mol/L after treated for 192 h (P<0.05).Treatment with triptorelin for 192 h at concentration of 10^-4 mol/L had no statistical significance effect on phosphorylation of ERK1/2 and Akt(P>0.05).CONCLUSION: Inhibitory effect of GnRH analogue triptorelin on human breast cancer cells is not just the connection with the down-regulation of pituitary hormone, but also a direct inhibitory effect. The role may not be involved in the activation of ERK/MAPK and PI3K/Akt signaling pathways.  相似文献