Comparison of the canine corneal epithelial cell sheets cultivated from limbal stem cells on canine amniotic membrane,atelocollagen gel,and temperature‐responsive culture dish          下载免费PDF全文

Eunryel Nam  Naoki Fujita  Maresuke Morita  Keiko Tsuzuki  Hsing Yi Lin  Cheng Shu Chung  Takayuki Nakagawa  Ryohei Nishimura 《Veterinary ophthalmology》2015,18(4):317-325

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Clinical,histopathological and immunohistochemical characterization of a novel equine ocular disorder: heterochromic iridocyclitis with secondary keratitis in adult horses          下载免费PDF全文

Nelson I. Pinto  Richard J. McMullen Jr.  Keith E. Linder  John M. Cullen  Brian C. Gilger 《Veterinary ophthalmology》2015,18(6):443-456

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大葱种子人工老化与膜脂过氧化的研究   总被引：3，自引：0，他引：3  

李颜  王倩 《种子》2007,26(3):27-30

大葱种子经过人工老化处理后,其发芽率、发芽指数、活力指数下降,活力指数较发芽率和发芽指数下降明显,种子老化6天后活力指数比对照下降70％。脱氢酶、SOD、POD活性和GSH含量也呈下降趋势,而浸出液可溶性糖、MDA、自由基、H2O2的含量增加,GSH、H2O2、自由基、可溶性糖含量与种子活力显著相关,并且较其他指标变化更为显著。说明大葱种子人工老化过程中膜脂过氧化加剧,膜结构和功能遭到破坏。因此,大葱种子人工老化与膜脂过氧化直接相关。  相似文献   

Witnessed Cardiac Arrest in a Hypothermic Avalanche Victim Completely Buried for 2 Hours     

Nicholas Daniel  Nicholas Weinberg  Frank Carus  Benjamin Church  Ken Zafren 《The Forestry Chronicle》2021,32(1):92-97

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外源Ca²⁺对模拟酸雨胁迫下不同抗性水稻根系氮吸收的影响     

张元棋  梁婵娟 《农业环境科学学报》2022,41(11):2468-2477

为减轻酸雨对植物生长的不利影响,探究Ca²⁺对植物耐酸性的调控机制,本文以五优308（抗性种）和南粳9108（敏感种）两个品种水稻为研究对象,研究外源Ca²⁺对低强度酸雨（pH 4.5,SAR1）和高强度酸雨（pH 3.0,SAR2）胁迫下水稻幼苗根系生长、氮（NO₃^-和NH₄⁺）含量及吸收速率、ATP含量、质膜H⁺-ATPase活性及其磷酸化水平的影响。结果表明： SAR1处理下两个品种水稻的H⁺-ATPase磷酸化水平和活性增加（P<0.05）,促进ATP分解,增加供能,使NH₄⁺吸收增加（P<0.05）,但NH₄⁺仅在南粳9108根中过量积累（P<0.05）,引起铵毒,造成根系生长抑制,五优308中NO₃^-和NH₄⁺含量及其生长均未受影响（P>0.05）。SAR2处理下,两个品种水稻质膜H⁺-ATPase活性和NO₃^-、NH₄⁺吸收速率及含量均降低,根系生长受到抑制（P<0.05）,其中南粳9108降幅大于五优308。Ca²⁺+SAR1处理组两个品种水稻根系质膜H⁺-ATPase磷酸化水平和活性、NO₃^-和NH₄⁺吸收和积累以及根系生长均与对照（叶喷去离子水处理）差异不显著（P>0.05）。Ca²⁺+SAR2处理下H⁺-ATPase活性、NO₃^-和NH₄⁺吸收和积累以及根系生长低于对照（P<0.05）,但显著高于单一SAR2处理（P<0.05）。研究表明,外源Ca²⁺可有效保障模拟酸雨（pH 4.5、3.0）下质膜H⁺-ATPase磷酸化水平,促进H⁺-ATPase活性升高,缓解酸雨对NO₃^-和NH₄⁺吸收的抑制,维持根系生长。其中,外源Ca²⁺对相同强度模拟酸雨胁迫下五优308的调控效果优于南粳9108,说明外源Ca²⁺对酸雨胁迫下植物氮吸收的影响不仅受酸雨强度限制,而且也会受品种影响。本实验中,外源Ca²⁺对不同强度酸雨胁迫下不同抗性水稻氮吸收均有调控效果,合理利用外源Ca²⁺将有助于调节酸雨区农作物的营养吸收,缓解酸雨对农业生产的危害。  相似文献   

农田残膜机械回收膜土分离装置设计与试验     

胡广发  全腊珍  邹运梅  沈徽  匡新鹏  黄从辉 《中国农业科技导报》2021,23(7):82-92

在我国南方农田残膜回收作业过程中,因土壤粘性强导致残膜夹带土壤多,残膜极易缠绕在机具上,影响机具正常工作。针对该问题设计了一种膜土分离装置,该装置主要由挑膜弹齿、滚筒和凸轮压板机构组成,可在挑膜过程中去除膜上表层土壤并在送膜过程中去除膜下土壤,通过试验分析得到影响膜土分离的主要因素为机具前进速度、挑膜转速和滚筒安装倾角。确定以上述三个因素为试验因素,以含土率和缠膜率为响应值,进行三因素三水平响应面试验。利用Desige-Expert软件建立各因素与缠膜率、含土率的回归模型,分析各因素对回收效率的影响,结果表明,各因素对含土率的显著性顺序为：机具前进速度＞挑膜转速＞滚筒安装倾角;对缠膜率的显著性顺序为：滚筒安装倾角＞挑膜转速＞机具前进速度;同时,对试验因素进行优化,得到因素的最佳组合为：机具前进速度为1.12 m·s^-1 ,挑膜转速为92.00 r·min-1,滚筒安装倾角为17.00°,此时的含土率为13.00%,缠膜率为1.70%。通过土槽模拟试验,设置上述最佳工作参数组合,得到含土率为13.45%,缠膜率为1.78%,验证结果与模型优化结果相对误差都小于5%。该膜土分离装置可以解决南方粘性土壤膜土分离困难、残膜率高的问题,为后续膜土分离装置及残膜回收机的设计与优化提供参考。  相似文献   

鳗弧菌、溶藻胶弧菌外膜蛋白的分离及特性   总被引：16，自引：3，他引：16       下载免费PDF全文

周丽  刘洪明  战文斌  宋微波 《中国水产科学》2003,10(1):31-35

分别用SDS、Sarkosyl及PMSF法分离制备鳗弧菌（Vibrio anguillarum）、溶藻胶弧菌（Vibrio alginolyticus）主要外膜蛋白（MOMP），通过SDS-PAGE分析比较2种弧菌主要外膜蛋白的组成结构。结果表明，SDS、Sarkosyl和PMSF法分离提取的鳗弧菌和溶藻胶弧菌外膜蛋白中，SDS-PAGE电泳图谱不同，鳗弧菌外膜蛋白分子量为27-138kD；溶藻胶弧菌外膜蛋白分子量为27-104kD，其中，45kD、30kD和27kD外膜蛋白为鳗弧菌和溶藻胶弧菌所共有。经比较，Sarkosyl法对2种弧菌外膜蛋白的提取效果较好。对3种方法提取的2种弧菌的主要外膜蛋白进行SDS PAGE后，分别与吸附后的兔抗鳗弧菌、抗溶藻胶弧菌血清的Western-blotting印迹显示，兔抗鳗弧菌血清与其菌株的外膜蛋白主要有3条免疫反应带，其分子量分别为97kD、51kD和30kD，说明其可能与鳗弧菌抗原的特异性有关；兔抗溶藻胶弧菌血清与其菌株的外膜蛋白主要有2条免疫反应带，其分子量分别为51kD和27kD，说明可能与溶藻胶弧菌抗原的特异性有关，而51kD外膜蛋白可能是2种弧菌共有的特异性抗原。  相似文献   

电磁场对几种淡水鱼鱼苗胚胎期的出苗率、抗温变能力和后期生长速度的影响   总被引：2，自引：0，他引：2  

叶士璟  万东辉  叶永青  罗文芸  江林华  章继敏  陈家森 《水产学报》1990,14(3):251-255

对蟾蜍、鱼类胚胎的质子核磁弛豫的研究发现,在胚胎发育期中的神经胚期出现质子纵向核磁弛豫时间T_1值为一极大值。几年来,我们用各种物理手段对蟾蜍和几种淡水鱼胚胎在不同发育期进行物理刺激,发现在鱼类胚胎早期和神经期接受较强电场或磁场刺激后,不仅其后期生长速度显著加快,而且胚胎发育期抗温变能力有明显提高,孵化出苗率也有较大幅度增加。1989年5月,我们在上海嘉定县望新鱼苗场对团头鲂早期胚胎进行了中等规模(10公斤受精卵)电场刺激试验,取得了令人满意的后期结果。  相似文献   

Anemia amelioration by lactose infusion during trypanosomosis could be associated with erythrocytes membrane de-galactosylation     

E.O. Balogun  J.B. Balogun  S. Yusuf  H.M. Inuwa  I.S. Ndams  P. Sheridan  D.K. Inaoka  T. Shiba  S. Harada  K. Kita  K.A.N. Esievo  A.J. Nok 《Veterinary parasitology》2014,199(3-4):259-263

African trypanosomosis is a potentially fatal disease that is caused by extracellular parasitic protists known as African trypanosomes. These parasites inhabit the blood stream of their mammalian hosts and produce a number of pathological features, amongst which is anemia. Etiology of the anemia has been partly attributed to an autoimmunity-like mediated erythrophagocytosis of de-sialylated red blood cells (dsRBCs) by macrophages. Lactose infusion to infected animals has proven effective at delaying progression of the anemia. However, the mechanism of this anemia prevention is yet to be well characterized. Here, the hypothesis of a likely induced further modification of the dsRBCs was investigated. RBC membrane galactose (RBC m-GAL) and packed cell volume (PCV) were measured during the course of experimental trypanosomosis in mice infected with Trypanosoma congolense (stb 212). Intriguingly, while the membrane galactose on the RBCs of infected and lactose-treated mice (group D) decreased as a function of parasitemia, that of the lactose-untreated infected group (group C) remained relatively constant, as was recorded for the uninfected lactose-treated control (group B) animals. At the peak of infection, the respective cumulative percent decrease in PCV and membrane galactose were 30 and 185 for group D, and 84 and 13 for group C. From this observed inverse relationship between RBCs membrane galactose and PCV, it is logical to rationalize that the delay of anemia progression during trypanosomosis produced by lactose might have resulted from an induction of galactose depletion from dsRBCs, thereby preventing their recognition by the macrophages.  相似文献   

Relationship of Sperm Quality to Fertility after 4 Days of Cooled Storage of Equine Semen     

Allison M. Kiser  Steven P. Brinsko  Charles C. Love  Dickson D. Varner  Kendrick Sudderth  Terry L. Blanchard 《Journal of Equine Veterinary Science》2014

This study evaluated measures of sperm quality in relation to fertility achieved with fresh semen or semen cooled and stored. Semen from 1 stallion was collected and processed to provide 3 treatments: group 1 received fresh semen; group 2 received cooled semen containing 50% seminal plasma (SP) stored for 4 days; and group 3 received cooled semen containing 50% SP stored for 1 day, then centrifuged and resuspended in fresh extender containing 10% SP on days 1 to 3. Inseminates were evaluated for sperm motion characteristics and the percentage of sperm with intact membranes (SMI). Mares (n = 34) in estrus were treated with an ovulation-inducing drug and inseminated with 100 million membrane-intact sperm on the following day. Pregnancy status was determined via transrectal ultrasonography 2 weeks after ovulation. The mean percentage of SMI was higher in group 1 (81%, initial) than in group 2 (74%, day 4) or group 3 (74%, day 4) (P < .05). The median percentages of total sperm motility differed among the groups (77%, 5%, 59% for groups 1, 2, and 3 respectively; P < .05). Median values for the percentages of progressively motile sperm and curvilinear velocity for group 1 (55%, 216 μm/s) and 3 (37%, 186 μm/s) were higher than for group 2 (1%, 73 μm/s) (P < .05). Pregnancy rates did not differ among groups (5 of 11, 45% in group 1; 5 of 11, 45% in group 2; and 7 of 12, 58%, in group 3; P = .77). These data suggest that, at least for this stallion, sperm membrane integrity may be a more valuable means of assessing potential fertility of cooled-stored semen than sperm motion characteristics.  相似文献