全文获取类型
收费全文 | 1981篇 |
免费 | 114篇 |
国内免费 | 195篇 |
专业分类
林业 | 101篇 |
农学 | 171篇 |
基础科学 | 90篇 |
225篇 | |
综合类 | 828篇 |
农作物 | 111篇 |
水产渔业 | 98篇 |
畜牧兽医 | 471篇 |
园艺 | 88篇 |
植物保护 | 107篇 |
出版年
2024年 | 11篇 |
2023年 | 23篇 |
2022年 | 52篇 |
2021年 | 48篇 |
2020年 | 56篇 |
2019年 | 55篇 |
2018年 | 39篇 |
2017年 | 62篇 |
2016年 | 91篇 |
2015年 | 62篇 |
2014年 | 95篇 |
2013年 | 135篇 |
2012年 | 139篇 |
2011年 | 125篇 |
2010年 | 109篇 |
2009年 | 120篇 |
2008年 | 103篇 |
2007年 | 159篇 |
2006年 | 115篇 |
2005年 | 99篇 |
2004年 | 74篇 |
2003年 | 69篇 |
2002年 | 65篇 |
2001年 | 55篇 |
2000年 | 50篇 |
1999年 | 48篇 |
1998年 | 39篇 |
1997年 | 28篇 |
1996年 | 35篇 |
1995年 | 33篇 |
1994年 | 17篇 |
1993年 | 13篇 |
1992年 | 15篇 |
1991年 | 11篇 |
1990年 | 12篇 |
1989年 | 4篇 |
1988年 | 11篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1983年 | 2篇 |
1980年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1963年 | 1篇 |
1956年 | 1篇 |
排序方式: 共有2290条查询结果,搜索用时 15 毫秒
71.
副猪嗜血杆菌外膜蛋白P5能诱导机体产生免疫保护反应,同时可以用于特异性的血清学诊断,本试验选取P5蛋白进行抗原表位鉴定。首先通过PCR扩增P5F1(1~204aa),P5F2(170~296aa)及P5F3(280~371aa)3个片段,PCR产物分别定向克隆到表达载体pET32a(+)中表达纯化。根据ELISA和Western blotting结果确定P5F3片段(280—371aa)是OmpP5的免疫优势决定区。为了进-步对该免疫优势决定区进行抗原表住鉴定,设计了-套11个部分重叠的短肽,这些短肽覆盖全部280~371aa片段。每-个短肽合成1对寡核苷酸链,退火后插入表达载体pGEX-6p-1,与GST进行融合表达。用HPS阳性血清进行ELISA和Western blotting扫描,鉴定出其表位位于”。TGNTCDAVKGRKALIT351。通过序列分析证实该抗原表位在不同的HPS菌株中高度保守。本试验确定了位于HPSOmpP5上的-个抗原表位,为建立-种方便、快捷、适用于现地大规模样品检测的鉴别诊断方法奠定了基础,同时也为HPS新型亚单位疫苗的研制,以及研究病原茵感染和机体免疫过程中P5蛋白与宿主体内相应分子之间的相互作用提供了有用信息。 相似文献
72.
36只体质量约为20kg的山羊被随机分为4个组,每组9只,试验组灌服相同量的CdCl2和不同量的[(NH4)6Mo7·O24·4H2O],对照组灌服对应量去离子水。按每千克体质量计算,低钼组(0.5mg/kg(Cd)+15mg/kg(Mo))、中钼组(0.5mg/kg(Cd)+30mg/kg(Mo))、高钼组(0.5mg/kg(Cd)+45mg/kg(Mo)),每组3个重复。于试验的第0、10、20、30、40、50天每组山羊采血,于试验的第0、25、50天每组山羊取脾脏和肝脏,测定相关指标。结果显示:(1)随着试验时间的延长,各试验组红细胞膜MDA含量呈上升趋势,GSH-Px、GST、SOD活性呈下降趋势;在第50天时,与对照组相比,各试验组红细胞膜MDA含量上升(P〈0.01),GSH-Px、GST、SOD活性降低(P〈0.01);(2)与对照组相比,高钼组脾脏的脾小体体积减小,可见散在的含铁血黄素沉积;(3)随着试验时间的延长,各试验组肝脏Bax基因表达量呈上升趋势;在第50天时,与对照组相比,低钼组和高钼组Bax表达量上升(P〈0.05)。结果表明,钼可导致镉胁迫下山羊红细胞膜自由基代谢紊乱,并能损害脾脏的免疫功能并且上调肝Bax基因的表达量,钼和镉体现为协同作用。 相似文献
73.
就气单胞菌外膜蛋白的基因工程亚单位疫苗、DNA疫苗、重组活载体疫苗等基因工程疫苗的研究现状、免疫方式以及不足之处进行了综述,以期为气单胞菌疫苗研制提供参考。 相似文献
74.
We investigated the influence of different levels of osmotic stress on growth and development in selected wild almond species(eight Prunus spp.) grown in vitro. The study, while endorsing the efficacy of in vitro screening of auxiliary buds of wild almond for osmotic stress tolerance, showed species variability in its response to osmotic stress. Osmotic stress reduced growth and development of all the species. However, the putative tolerant Prunus spp. showed better performance than the putative susceptible genotypes. On average there was an 80% decrease in shoot dry weight at-1.2 MPa. Reduction in shoot weight was more common in osmotic stress-susceptible species in the section labeled ‘Euamygdalus'. The tolerant Prunus species produced smaller changes in biochemical responses than the sensitive cultivars for malondialdehyde content, catalase activity, relative permeability of protoplast membranes, and net photosynthetic rate. The tolerant species maintained cell integrity better than drought sensitive species. Wild almond species in the section labeled ‘Spartioides'(Prunus arabica(Olivier) Neikle, Prunus glauca (Browicz) A.E. Murray, Prunus scoparia Spach) and ‘Lycioides'(Prunus lycioides Spach, Prunus reuteri Bossi. et Bushe) were best adapted to osmotic stress. Increase in chlorophyll concentration and leaf thickness under high osmotic stress can be considered as preliminary selection parameters for osmotic stress tolerance in Prunus spp. The study confirmed the efficacy of the in vitro method for screening of large number of genotypes for osmotic stress tolerance in wild almond species. 相似文献
75.
76.
77.
Blood, freshly collected from warm- and cold-acclimated carp, Cyprinus carpio L., was cooled to 5°C for 4h or warmed to 25°C for 4h, respectively, and the fluorescence anisotropy of washed red blood cells was recorded using the fluorescent dye 3-(p-(6-phenyl-1,3,5-hexatrienyl) phenyl propionic acid [DPH-PA] (which is restricted to the outer leaflet of the plasma membrane) before and after the temperature shift. Despite individual variation, the plasma membrane of cold-exposed erythrocytes became more fluid while that of warm-exposed cells became more rigid following the temperature shift. This response was rapid and reversible. Cold-exposed cells from warm-acclimated fish became more fluid within 40–60 minutes and reverted to their original fluidity within the same time on warming, at a rate of 1°C/min; erythrocytes, from cold-adapted carp displayed an opposite change in fluidity over a similar time period. Cells from warm-acclimated, temperature down-shifted carp hyperfluidized their plasma membranes in the cold, whereas cells from cold-acclimated fish up-shifted in temperature showed no similar effect. These cells showed a complete adjustment of membrane physical state to the temperature. Total phospholipids obtained from warm-acclimated temperature down-shifted cells became more rigid than they were, when assayed at the acclimation temperature. In contrast, phospholipids obtained from cold-acclimated cells became more rigid when exposed to increasing temperatures. No significant changes occurred to the polar head groups, or to the fatty acid composition of the total phospholipids. It was concluded that the lipids play only a secondary role in the control of the physical state of plasma membrane in carp erythrocytes, and that some non-lipid components of these structures might be involved in these regulatory processes. 相似文献
78.
Dietary lipid level affects fatty acid composition and hydrolase activities of intestinal brush border membrane in seabass 总被引:2,自引:0,他引:2
C.L. Cahu J.L. Zambonino Infante G. Corraze D. Coves 《Fish physiology and biochemistry》2000,23(2):165-172
Triplicate groups of juvenile seabass (initial weight of 241 g) were fed during 13 weeks three isonitrogenous experimental diets containing different lipid levels, 12% (LL group), 21% (ML group) and 30% (HL group). At the end of the experiment, fish weight gain was similar among the 3 dietary groups. Intestinal brush border membranes were purified for each dietary group; one part of the brush border fraction was dedicated to enzyme assays, the remaining fraction being used for lipid extraction followed by fatty acid analysis. The fatty acid composition of the brush border membrane differed among the 3 groups, although the 3 experimental diets had the same fatty acid composition. The increase in dietary lipid level resulted in a lowering in (n-3) polyunsaturated fatty acid (PUFA) paralleled with an increase in monounsaturated fatty acid. A significant reduction in the brush border enzyme activities, namely alkaline phosphatase, aminopeptidase N, -glutamyl transpeptidase and maltase, was also observed with the elevation of the dietary lipid level. The change in activity of intestinal digestive enzymes, which are membrane-bound proteins, could be attributed to the modification of fatty acid composition and fluidity of the brush border membranes (BBM). Such lowering in PUFA and increase in monounsaturated fatty acid in BBM, concomitant with a decline in membrane enzymatic activity, has been described as a malnutrition indicator in mammals. It raises the question of possible disorders of gut functions in fish fed increasing lipid levels. 相似文献
79.
精子膜蛋白受精素(Fertilin)是参与精卵质膜间相互作用最有特征性的候选分子之一。受精素由α、β两个亚基组成,为精子表面的异二聚体跨膜糖蛋白。fertilin-α(Fα)、Fertilin-β(Fβ)可能在精子发生、精子成熟、精子获能及精卵结合和融合中发挥重要作用。 相似文献
80.