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101.
从广西各地患禽巴氏杆菌病急性死亡的鸡、鸭肝脏分离出56株禽巴氏杆菌强毒株,经培养特性和生化特性试验鉴定,从中选出15株典型禽巴氏杆菌菌株。Carter荚膜抗原分型鉴定,15株菌均为荚膜A型,间接血凝滴度为1∶160~1∶640。通过毒力和免疫原性测定,从中选择毒力较强、免疫原性较好的B25、B26、B273菌株进行人工致弱,其中B26菌株在0.1%裂解全血马丁汤中,通过物理诱变方法致弱,即在传代过程中,把培养温度由37℃逐渐提高到45℃,每12h传1代而成功致弱,传至1200代时,毒力显著减弱,且仍保持其良好的免疫原性和培养特性,从而获得了禽巴氏杆菌B26-T1200弱毒菌株 相似文献
102.
HANG Tianyu ZHAO Hongzhe SONG Qianjin ZHANG Jing JI Zhi WEN Yongjun GUAN Pingyuan 《中国畜牧兽医》2007,47(11):3641-3650
In this study,the genome of Brucella Rev.1 strain was used as a template to amplify the BAB gene sequence,clone it into the prokaryotic expression vector pET-30a(+),obtain the recombinant plasmid pET-30a-BAB,and perform prokaryotic expression on the recombinant plasmid.The indirect ELISA method was constructed by using the expression products detected by Western blotting.The results showed that the BAB gene was successfully cloned and expressed in this experiment,and the purified expression product was analyzed by SDS-PAGE.This study obtained a relatively pure recombinant BAB protein;Western blotting test showed that the expressed protein could react specifically with Brucella sheep positive serum and had good reactogenicity;Using the recombinant BAB protein as the coating antigen,an indirect ELISA method for detecting BAB antibodies was established and optimized.The best determined coating conditions were as follows BAB protein coating amount was 0.25 μg/mL,serum dilution was 1:400;blocking solution was 3% pig-derived gelatin;secondary antibody dilution was 1:6 000;color development time was 10 min.The established method was used to detect 40 clinical sheep serums,and the cut-off value was calculated to be 0.607.That was,when the serum tested had P/N ≥ 1.5 and D450 nm ≥ 0.607,it was judged as positive,when D450 nm ≤ 0.561,it was judged as negative,and when 0.607<D450 nm<0.561,it was judged as a suspect value,and retest was required.Compared with the Huhong plate test and the test tube agglutination test,the positive coincidence rate was 100%,the negative coincidence rate was 71.88%,and the total coincidence rate was 77.5%. 相似文献
103.
研究以鸡传染性喉气管炎病毒(ILTV)烟台株为模板,PCIK扩增出1条约2.7的gB基因片段,并将其克隆到pMD18-T载体中。序列测定显示,gB基因长2622bp,包含完的阅读框架。序列比较分析发现,烟台株和王岗株、河北株的班基因核苷酸序列与SA2株的比均在第89位上缺失1个碱基G,而在第102位核苷酸处插入1个碱基A,从而引起氨基酸序中第29-32位5个氨基酸的移码突变。烟台株还有另外7个碱基发生突变,使得其班基因与北株、王岗株、SA2疫苗株的gB基因核苷酸同源性分别为99.8%、99.7%和99.6%,氨基酸的同性分别为99.4%、99.4%、98.9%,表明不同ILTV毒株之间gB基因是非常保守的。划型相列河源 相似文献
104.
105.
研究表明:种衣剂处理对辣椒疫霉菌的侵染过程及辣椒植株的防御酶系均有影响。包衣处理的叶片上休止胞的萌发畸形,不形成附着胞,无力侵入寄主,侵入时间也比对照明显滞后,菌丝在寄主组织内的扩展速度及孢子囊产生的数量明显受到抑制。接种后,过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)比对照均有不同程度的提高,出现活性高峰后开始下降。表明甲霜灵对寄主植物抗性的影响具有一定的时间性,且与疫霉菌的激发有关。 相似文献
106.
为了了解同一猪场不同来源具相同耐药谱的11株大肠杆菌之间的同源性,以探讨其耐药流行机制,用脉冲场凝胶电泳(PFGE)对同一猪场不同来源具相同耐药谱的大肠杆菌做染色体DNA分型.结果发现分别来自小猪和环境的2株大肠杆菌通过PFGE分型,属于同一个亚型.初步认为不同来源的大肠杆菌来自不同克隆系,同时在动物环境之间可能存在着耐药性的克隆传播.结果表明,PFGE可作为耐药性流行病学调查的一种研究方法. 相似文献
107.
Reasons for performing study: Radial strain in normal hooves has been found to vary with strain gauge location, limb posture and sample limb but reported magnitudes were considered to be low. More accurate measurement of radial strain may enhance the understanding of hoof function. Objectives: To explore in vitro radial hoof strain in relation other kinetic and kinematic variables that may be related. Methods: Five normal forelimbs were removed at the proximal articular surface of the third metacarpal bone (McIII). The limbs were loaded using a modified Instron test machine. Six calibrated infrared cameras captured movement from markers on the hoof and bone fixed markers on the second and first phalanxes and McIII, whilst radial hoof strain was measured using a calibrated instrumented plug. Change in strain, joint angle and load were found at simulated walking postures and bivariate correlations were used to compare the relationships between them. Results: Radial strain was moderately correlated with proximal interphalangeal joint (PIPJ) rotation (r =?0.519). Large reductions in radial strain were found in loading and midstance with 10° of heel lift postures. Conclusions and potential relevance: PIPJ rotation has previously been linked to the magnitude of deep digital flexor tendon (DDFT) loads and it is therefore suspected that these loads may have the greatest influence on radial strain magnitudes. Further investigation of radial strain is needed to describe the patterns fully during the stance phase in vivo. 相似文献
108.
洪永聪 《青岛农业大学学报(自然科学版)》2009,26(3):181-183
在大田甘蓝地中,以从菜园土壤和菠菜叶片分离得到的氯氰菊酯降解菌株T-1和菌株Y-3为研究对象,采用气相色谱法检测,对降解菌降解氯氰菊酯的残留动态进行研究。结果表明:与对照相比,降解菌对氯氰菊酯的降解有促进作用;菌株Y-3和菌株T-1在土壤中对氯氰菊酯的降解动态基本类似,半衰期均为10d;具有内生性的菌株Y-3在甘蓝植株体内对氯氰菊酯降解效果较好,半衰期为7d。 相似文献
109.
110.
普通油茶对炭疽病抗性的病理学研究张常青关键词普通油茶、油茶炭疽菌、抗病类型、孢子萌发与侵入由于油茶炭疽病菌(ColetotrichumcameliaeMassee)的危害,使普通油茶(CameliaoleifelaAbol)大量落花落果,这是严重影响... 相似文献