马铃薯群体B品系对晚疫病水平抗性遗传稳定性试验     

罗文富  杨艳丽  王军  宋伯符  王毅  赵庆云 《云南农业大学学报(自然科学版)》2000,15(3):205-207

 1997～1999年,在昆明地区对马铃薯中心提供的马铃薯群体B育种材料进行了晚疫病水平抗性遗传稳定性的田间检验。实验采用完全随机区组（RCBD）的方法,在当地晚疫病菌生理小种存在的条件下,经过3年的田间小区试验筛选,获得了Pn-01、Pn-06等表现水平抗性遗传稳定性与优良农艺性状相结合的品系,为进—步进行试验示范和大面积推广打下了基础。  相似文献   

番茄芽枯病现状调查及其防治     

唐树发  崔立群  陆丽莹  王金国  唐昊  邵桂珍 《杂粮作物》2012,(12)

针对番茄芽枯病发生现状，在阐述该病害表现症状的基础上，分析了其发病特点及原因，包括水分供应不足、高温引起水分失衡、高温烫伤及微量元素不足等，并从培养无病壮苗、定植管理、水分管理、蹲苗及中午通风遮阳等方面论述了番茄芽枯病的防治方法.  相似文献   

Development and Identification of Introgression Lines from Cross of Oryza sativa and Oryza minuta     

GUO Si-bin  WEI Yu  LI Xiao-qiong  LIU Kai-qiang  HUANG Feng-kuan  CHEN Cai-hong  GAO Guo-qing 《水稻科学》2013,20(2):95-102

Introgression line population is effectively used in mapping quantitative trait loci(QTLs),identifying favorable genes,discovering hidden genetic variation,evaluating the action or interaction of QTLs in multiple conditions and providing the favorable experimental materials for plant breeding and genetic research.In this study,an advanced backcross and consecutive selfing strategy was used to develop introgression lines(ILs),which derived from an accession of Oryza minuta(accession No.101133) with BBCC genome,as the donor,and an elite indica cultivar IR24(O.sativa),as the recipient.Introgression segments from O.minuta were screened using 164 polymorphic simple sequence repeat(SSR) markers in the genome of each IL.Introgressed segments carried by 131 ILs covered the whole O.sativa genome.The average number of homozygous O.minuta segments per introgression line was about 9.99.The average length of introgressed segments was approximate 14.78 cM,and about 79.64% of these segments had sizes less than 20 cM.In the genome of each introgression line,the O.minuta chromosomal segments harbored chromosomal fragments of O.sativa ranging from 1.15% to 27.6%,with an overall average of 8.57%.At each locus,the ratio of substitution of O.minuta alleles had a range of 1.5% 25.2%,with an average of 8.3%.Based on the evaluation of the phenotype of these ILs,a wide range of alterations in morphological and yield-related traits were found.After inoculation,ILs 41,11 and 7 showed high resistance to bacterial blight,brown planthopper and whitebacked planthopper,respectively.These O.minuta-O.sativa ILs will serve as genetic materials for identifying and using favorable genes from O.minuta.  相似文献   

Gene Expression of Enzymes for Starch and Sucrose Metabolism and Transport in Leaf Sheaths of Rice (Oryza sativa L.) during the Heading Period in Relation to the Sink to Source Transition     

《Plant Production Science》2013,16(3):178-183

Abstract

Leaf sheaths of rice plants are known to temporarily accumulate starch prior to heading, which is subsequently remobilized and transported into the panicle after heading. We investigated the time course for both carbohydrate content and steady state mRNA levels of enzymes related to starch and sucrose metabolism in the rice leaf sheath (Oryza sativa L. cv. Nipponbare). Leaf sheaths from the second leaf below the flag leaf accumulated high levels of starch before heading but they rapidly decreased after heading. In contrast, the flag leaf sheath did not accumulate as much starch. In the second leaf sheath, the mRNA levels of enzymes involved in starch synthesis, ADP glucose pyrophosphorylase (EC 2. 7. 7. 27), soluble starch synthase (EC 2. 4. 1.21) and branching enzyme (EC 2. 4. 1. 18) were high before heading, which coincided with rapid accumulation of starch. The mRNA levels of sucrose synthesis enzymes, cytosolic FBPase (EC 3. 1. 3. 11) and sucrose phosphate synthase (EC 2. 4. 1. 14), and the sucrose transporter (OsSUTI) increased at the time of heading, which was largely coincident with a decrease in the mRNA levels of starch synthesis enzymes. In the flag leaf sheaths, changes in mRNA levels of starch synthesis enzymes were not pronounced, however mRNA levels of sucrose synthesis enzymes and the sucrose transporter showed a clear increase throughout the heading period. The different characteristics observed between the two leaf sheaths will be discussed in relation to the sink to source transition.  相似文献   

适宜马铃薯晚疫病菌生长的豆类培养基筛选   总被引：5，自引：0，他引：5  

姚裕琪  梁德霖 《华北农学报》1998,13(3):88-90

结合晚疫病菌生理小种的鉴定工作，对用于该菌生长的豆类培养基进行了比较，人中选出日本菜豆制作的培养基，在对晚疫病菌菌株的分离、培养、保存等方面均取得了满意的效果，对开展晚疫病菌的研究工作十分有利。  相似文献   

苏丹草小斑病病原鉴定   总被引：3，自引：0，他引：3  

吴大椿  张传清  吴小刚 《湖北农学院学报》2000,20(4):308-311

通过对苏丹草新病害苏丹草小斑病病原与玉米小斑病菌和苏丹草大斑病菌的比较研究，鉴定出苏丹草小斑病病原与玉米小斑病病菌是同一个种，不玉蜀黍平脐蠕孢〔Ｂｉｐｏｌａｒｉｓ ｍａｙｄｉｓ（Ｎｉｓｈｉｋａｄｏ＆Ｍｉｙａｂｅ）Ｓｈｏｅｍａｌｃｅｒ＝Ｈｅｌｍｉｎｔｈｏｓｐｏｒｉｕｍ ｍａｙｄｉｓ Ｎｉｓｈｉｋａｄｏ＆Ｍｉｙａｂｅ＝Ｃｏｃｈｌｉｏｂｏｌｕｓ ｈｅｔｅｒｏｓｔｒｏｐｈｕｓ（Ｄｒｅｃｈｓ．）Ｄｒｅｃｈｓｌｅｒ（有性世代）〕，苏丹草是一种新寄主。  相似文献   

桉树焦枯病病原菌特性的观察   总被引：6，自引：0，他引：6  

邓玉森  岑炳沾 《广东林业科技》1997,13(1):30-35

对桉树焦枯病病原菌３种典型的５隔膜、３隔膜和１隔膜分生孢子进行了研究。５隔膜病原菌致病力最强，３隔膜次之，１隔膜较弱。匀菌剂菌毒清对３种病原菌有明显的抑制作用。  相似文献   

Description of an ultrasound-guided rectus sheath block injection technique and the spread of dye in calf cadavers     

《Veterinary anaesthesia and analgesia》2022,49(2):203-209

ObjectivesTo describe an ultrasound-guided rectus sheath (RS) block technique in calves with injections of methylene blue and assess the extent of injectate spread and nerve staining in calf cadavers.Study designProspective, experimental, blinded cadaveric study.AnimalsA total of 16 calf cadavers weighing 30.7 ± 7.1 kg.MethodsUsing an ultrasound-guided, in-plane technique, each cadaver was injected with both a low (LV; 0.25 mL kg^–1) and high (HV; 0.5 mL kg^–1) volume of methylene blue dye. Volumes were randomly assigned to the left or right hemiabdomen. Ultrasound imaging was scored based on landmarks and needle visualization, and duration to perform injections were assessed. Dissections were performed immediately after injections. Staining of ventral branches of spinal nerves and the extent of dye spread were recorded by an anatomist unaware of treatment allocation.ResultsThe number of nerves stained in treatments LV and HV were 2.3 ± 1.7 and 4.4 ± 1.3, respectively (p = 0.0001). Branches of thoracic nerves T7–13 and lumbar nerves L1–2 were completely stained 6.3%, 6.3%, 31.3%, 62.5%, 56.3%, 37.5%, 31.3%, 6.3% and 0%, respectively, in treatment LV and 0%, 12.5%, 50%, 93.8%, 100%, 93.8%, 62.5%, 25% and 6.3%, respectively, in treatment HV. Ultrasound imaging was scored excellent in most cadavers and needle visualization deemed excellent in all injections. The mean duration to perform RS injections in both treatments was 2 (range, 1–6) minutes.Conclusions and clinical relevanceNerve staining results from treatment HV suggest that this RS injection technique could be clinically useful in ventral midline surgical procedures, including umbilical procedures, in calves. This study supports future clinical trials in calves.  相似文献   

油桐抗枯萎病株系的选鉴研究^*   总被引：1，自引：0，他引：1       下载免费PDF全文

花锁龙 《林业科学研究》1991,4(2):160-166

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千家坪林区油松赤枯病调查及对策     

梁宏胜  陈文普  姜勇 《陕西林业科技》2004,(3):34-37

本文调查了陕西省平利县千家坪林区油松赤枯病的发生和危害情况，在调查的818．4hm^2油松人工林中，发病611hm^2，占74．4％。同时对赤枯病的发生特点进行了调查，结果表明该病的发生与树龄、海拔、坡向有一定的关系。在高海拔地区应该采取更新树种，低海拔地区进行疏伐和化学防治的策略。  相似文献