Characterization of Genomic Integration and Transgene Organization in Six Transgenic Rapeseed Events     

WU Yu-hua  ;ZHANG Li  ;WU Gang  ;NIE Shu-jing  ;LU Chang-ming 《中国农业科学(英文版)》2014,(9):1865-1876

To characterize the DNA rearrangement of both the T-DNA region and the genomic insertion site during T-DNA insertion, the Genomewalker strategy was used to isolate the junctions between the inserted DNA and the plant genomic DNA in six rapeseed events as well as the genomic DNA at the sites before integration. During transformation in each of the six events, portions of both the right border（RB） and left border（LB） regions of the T-DNA were deleted, ranging from a 7 nucleotide deletion of the LB repeats in event RF1 to a 207 bp deletion of the LB region in event RF2. For the six events, T-DNA integration resulted in a deletion at the target site spanning less than 100 bp. Sequence analysis indicated that the T-DNA was integrated into the coding region of various native rapeseed genes in events RF1 and RF2. Duplications of the genomic DNA target site were observed in events RF2, RF3 and Topas 19/2. And multimerization of transgenes was found in event Topas 19/2, in which, the T-DNA was integrated as a head-to-head（RB-to-RB） concatemer into the recipient genome. In event MS1, chromosomal translocation or a large target-site deletion may have occurred during T-DNA integration, which was identified due to a failure to amplify the presumptive insertion site based on the flanking rapeseed DNA sequences. Our results provide comprehensive data concerning transgene organization and the genomic context of the T-DNA in six rapeseed events, which can aid in the developing of insert fingerprinting and the monitoring of long-term genetic stability and potential unintended effects of transgenic events.  相似文献   

Effect of One-off Application of Poly Urea-Formaldehyde Fertilizer Under Reduced N Rate on Double Cropping Rice   总被引：2，自引：0，他引：2  

QiaoYi HUANG  Mu ZHANG  Xu HUANG  ShuanHu TANG  FaBao ZHANG  YuWan PANG  Qiong YI  Ping LI  HongTing FU 《中国农业科学》2018,51(20):3996-4006

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两系优质晚稻广两优7203的特征特性及配套栽培技术     

曲姗姗  邱六根  石宠良  温和楦  周卫营  程攀  阴云伙  徐晓明  陈龙  田发春  李土明  彭炳生 《中国稻米》2016,(4):92-93

广两优7203是中国水稻研究所和中国科学院遗传与发育生物学研究所用广占63S与中恢7203组配而成的强优势两系杂交稻组合,2013年通过浙江省审定,2014年通过国家审定。本文主要介绍了该组合在江西大面积试验示范的表现及高产栽培技术。  相似文献   

抗条纹叶枯病基因STV11的功能性分子标记建立及粳型抗源筛选     

毛艇  李旭  张战  付立东  李振宇 《中国水稻科学》2016,30(6):661-667

条纹叶枯病是水稻(Oryza sativa L.)上最严重的病害之一。STV11是从籼稻Kasalath中鉴定的条纹叶枯病抗性基因,根据Kasalath型条纹叶枯病基因STV11 KAS6个碱基缺失的功能性多态性序列差异,设计基于PCR的功能性分子标记qstv11。利用此标记对299份试验材料[5份抗病品种、5份感病品种、41份辽宁省育种材料、17份日本粳型材料、142份秋光/七山占重组自交系(群体A)、85份笹锦/Habataki//笹锦回交重组自交系(群体B)、群体亲本4份]进行基因型分析并进行抗病性鉴定。结果表明,qstv11能够较好区分抗感基因型,而且在测试材料中与条纹叶枯抗性整体呈现共分离。41份辽宁材料中鉴定出含有STV11-R(抗)材料1份;日本粳型材料该位点均为STV11-S(感);群体A和B中分别筛选出粳稻背景STV11-R型材料24份和6份。  相似文献   

Effects of Different Nitrogen Treatments on N use Efficiency and Grain Yield of Mechanically-transplanted Hybrid Rice     

Chang YE  Ke LIU  Shaowen LIU  Jian LU  Mengtao GAO  Bilin LU  Yunbo ZHANG 《农业科学与技术》2017,18(2)

In order to investigate effects of topdressing on grain yield and N use efficiency of mechanically-transplanted hybrid rice,this experiment was conducted with 2-line hybrid rice cultivars,Quanliangyou 1 and Quanliangyou 681 with 3 application levels of N fertilizer,No,N1,N2,respectively.The results showed that there were no significant differences in grain yields of the 2 varieties between treatments N1 and N2.It was indicated that N is not the factor which could limit grain yield within limited application levels of N fertilizer.High N fertilizer treatment could contribute to the formation of effective panicles and spikelets per panicle,but it could also lead to the decline in grain filling rate.It was suggested that lowering the amount of Nfertilizer could facilitate improvement of N-use efficiency.  相似文献   

水稻小粒密穗突变体sep1的遗传分析、基因定位与育种利用     

王跃星  吴昆  方云霞  陈红旗  倪深  付向东  朱旭东 《中国水稻科学》2014,28(3):229-234

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江苏省杂草稻Rc基因的单体型分析   总被引：1，自引：0，他引：1  

李潇艳  强胜  宋小玲  蔡堃  戴伟民 《中国水稻科学》2014,28(3):304-313

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云南籼稻小苗机插技术研究     

龙瑞平  冷升璨  赵丽娟  尹娇  杨久  李贵勇  夏琼梅  朱海平  张玉屏  杨从党 《中国稻米》2021,27(5):134-137

为了降低机插稻育秧、运秧劳动强度,在云南籼稻地区探索籼稻小苗机插技术。以吉优716为材料,设置160 g/盘（T1 ）和180 g/盘（T2）2个播种量处理,秧龄9 d,每667 m²大田用秧6盘。以常规播种量70 g/盘,15 d秧龄,每667 m²用秧15盘为对照,比较分析了不同处理间秧苗素质、栽插质量、茎蘖动态和产量的差异。结果表明,虽然密播小苗处理秧苗素质不如对照,并且缺丛率较高,但每丛基本苗数、各时期的茎蘖数和有效穗数均显著高于对照,最终产量均高于对照。较高的有效穗数是机插密播小苗技术增产的主要原因。其中,T1处理用种量比对照减少了8.57%,秧盘数比对照减少60%,但产量比对照增加了9.0%,节本增效的效果明显,具有较大的推广潜力。  相似文献   

The Effect of Phytase on Production Performance,Egg Quality,Calcium and Phosphorus Excretion,and Fatty Acids and Cholesterol Concentration in Hy-Line Layers Fed Diets Containing Rice Bran     

《The Journal of Applied Poultry Research》2019,28(3):688-698

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近期我国稻谷(米)供求趋势分析及发展预测与对策   总被引：3，自引：0，他引：3  

施能浦 《中国稻米》2015,(1):1-5

本文分析了20年来我国水稻生产、消费和贸易的变化,认为只有"保红线(即保种植面积3 000万hm2,稻谷总产2亿t)攻单产"才能保证我国稻谷95%以上的自给率。并预测了2030年我国稻谷的供求趋势,认为只要确保种植面积年增长率达0.4%、总产年增长率达1.0%,供求关系将趋平衡,并略有节余。为确保我国中期稻谷生产与消费趋于合理区间,提出了"六保"发展措施。  相似文献