Engineering of a Single Chain Variable Fragment Antibody Specific for the Citrus tristeza virus and its Expression in Escherichia coli and Nicotiana tabacum     

P. Galeffi  G. Giunta  S. Guida  C. Cantale 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(5):479-483

Citrus tristeza virus (CTV) is one of the most destructive citrus virus diseases in the world. The construction of an engineered antibody, EMBL accession number AJ278109, able to specifically recognize its antigen, i.e. the coat protein of CTV, directly on infected plant material without any purification or manipulation of the entire woody plant. The potential uses of this engineered antibody are discussed.  相似文献   

Isolation of Blast Fungal Cerebroside Elicitor-responsive Genes in Rice Plants     

Kazutoshi SAWADA  Michiaki IWATA 《Journal of General Plant Pathology》2002,68(2):128-133

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Tobacco Plants Transformed with an Untranslatable Form of the Coat Protein Gene of the Potato Virus Y are Resistant to Viral Infection     

Khaled Masmoudi  Ines Yacoubi  Afif Hassairi  Lobna Naeit Elarbi  Radhouane Ellouz 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(4):285-292

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Alternative Route of Infection for Bacterial Seedling Blight of Rice Caused by Burkholderia plantarii     

Hisayoshi MIYAGAWA  Hiroyoshi INOUE 《Journal of General Plant Pathology》2002,68(4):356-362

Burkholderia plantarii , the pathogen of bacterial seedling blight of rice, was detected in paddy water. Its concentration rose in July and August. The bacterial concentration in the paddy water was always higher along levees than at distances more than 5 m from levees. Confirmed to be released into water when graminaceous weeds were immersed, B. plantarii survived for at least 4 days at 30°C. B. plantarii was splashed at least 30 cm upward by rain splash in the field. Harvested seeds, which had been sprayed with B. plantarii released from graminaceous weeds at the flowering stage, retained the bacteria. Bacterial seedling blight occurred when the seeds were then sown in nursery boxes. These results indicated that graminaceous weeds growing on levees of paddy fields are a source of infection of the disease and that rice seeds are infected through the paddy water. Received 23 May 2002/ Accepted in revised form 1 September 2002  相似文献   

Growth and development of Cuscuta chinensis Lam. and its impact on selected crops     

Buddhi Marambe  Siril Wijesundara  Kushan Tennakoon  Dhammika Pindeniya  Chandana Jayasinghe 《Weed Biology and Management》2002,2(2):79-83

The growth and development of Cuscuta chinensis Lam., and the effects of the holoparasite on the growth and yield of tomato ( Lycopersicon esculentum ), chilli ( Capsicum annuum ) and rice ( Oryza sativa ) were investigated in pot experiments under glasshouse conditions. 'Mature' and 'immature' seeds of C. chinensis showed 15 and 47% germination, respectively. The independent life duration of seedlings from 'immature' seeds was 6 days shorter than 'mature' seeds (19 days). Cuscuta chinensis formed haustoria on chilli and tomato, but not on rice seedlings. Infection with three C. chinensis seedlings per host plant reduced leaf area, dry weight, carbon and nitrogen content by 78–84% and 27–44% in tomato and chilli plants, respectively. Dry weight of C. chinensis was 0.63 g per pot in tomato, and 0.24 g per pot in chilli. Carbon and nitrogen content of C. chinensis was 0.189 and 0.028 g per pot, respectively, when grown with tomato. When it was grown with chilli, the carbon and nitrogen content of C. chinensis was 0.001 and 0.007 g per pot, respectively. Cuscuta chinensis reduced the fruit yield of tomato and chilli by 72 and 29%, respectively. The results indicated that tomato is a preferred host of C. chinensis compared with chilli, and rice is not preferred. In the host–parasite associations, C. chinensis suppressed growth and yield of chilli and tomato by diverting the elaborated products of metabolism of the hosts.  相似文献   

Effect of submergence and naproanilide application on the growth of hemp sesbania and rice     

Kangetsu Hirase  William T. Molin 《Weed Biology and Management》2002,2(2):116-119

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应用生物源农药防治水稻病害   总被引：2，自引：0，他引：2  

薛元海 《江西植保》2002,25(3):68-71

在稻瘟病始见期天始，喷施4％春雷霉素二次可以控制病害；用3％克菌康1：400倍液喷雾，可以有效控制水稻白叶枯病的危害，在孕穗期初现病时开始，喷链霉素1000万单位二次，也能防治白叶枯病；在纹枯病始现期喷40％纹霉星，病指防效可达67－81％。  相似文献   

水稻不同品种或组合对甲磺隆耐药性差异的机制研究   总被引：2，自引：2，他引：2  

谭小平  柏连阳  肖启明  彭丽莎  周小毛 《农药学学报》2002,4(2):45-49

以水稻乙酰乳酸合成酶(ALS)比活力为研究对象,通过在离体和活体条件下比较其对甲磺隆的敏感性水平,探讨了水稻不同品种或组合的耐药性差异机制。结果表明,离体条件下不同水稻品种或组合ALS对甲磺隆均较敏感,当甲磺隆浓度为100 μg/L时,对ALS比活力抑制率达60%以上,且活力百分比与甲磺隆浓度对数成直线负相关关系,相关程度显著或极显著;活体条件下ALS活力均受到甲磺隆不同程度的抑制,但同一品种ALS活力不随甲磺隆浓度的变化而呈现规律性变化。在甲磺隆浓度相同时,活体条件下所有供试水稻品种ALS活力均显著高于离体条件下的ALS活力,影响程度与植株耐药性差异基本一致,说明甲磺隆在水稻体内存在较强代谢失活作用,代谢失活差异是水稻不同品种或组合对甲磺隆的耐药性差异机制。  相似文献   

新疆棉区转Bt基因棉对棉田主要害虫及其天敌种群数量的影响   总被引：19，自引：0，他引：19  

孙长贵  徐静  张青文  封红兵  汪飞  宋荣 《中国生物防治》2002,18(3):106-110

20 0 0～ 2 0 0 1年 ,在新疆棉区系统地研究了转Bt基因棉田主要害虫及其天敌的发生规律。结果表明 :转Bt基因棉对棉铃虫有良好的控制作用 ,发生高峰期百株落卵量和百株幼虫数量都显著低于常规对照 ,转Bt自控田和转Bt化防田棉蚜和棉盲蝽发生数量则显著高于常规棉对照田。在天敌方面 ,转Bt自控田和转Bt化防田较常规棉对照田 ,丽草蛉种群数量有所增加 ,而小花蝽和黑食蚜盲蝽等 5种捕食性和几种寄生性天敌数量都有所下降。转Bt基因棉田棉蚜和棉盲蝽等刺吸性害虫发生数量加重而多种天敌数量减少的趋势值得引起重视。鉴于新疆地区棉铃虫发生数量较低目前无需专门防治的特点 ,作者建议 :可以考虑推广农艺性状较好的常规棉品种并保护天敌 ,以达到控制害虫、丰产丰收的目的。  相似文献   

晚稻稻瘟病BP神经网络分区预报   总被引：2，自引：0，他引：2  

唐启义  程家安  蒋学辉  钱东兰 《植物保护学报》2002,29(3):205-209

应用相关分析方法分析了浙江省19个县1988～1999年晚稻稻瘟病发病与有关环境因子的关系,筛选了8个气象因子用于晚稻稻瘟病发生程度长期预报。根据各预报因子与稻瘟病发病程度相关性,采用邻接二维图论聚类分析法,将19个点(县)划分为4个生态区。每个生态区内运用BP神经网络技术建立模型,并进行拟合和试报。1997～1999年试报验证,在划分稻瘟病生态区的基础上,应用BP神经网络模型对稻瘟病进行长期预测预报是可行的,3年试报成功率分别是78.95％、84.21％和78.95％。文中还对该方法与过去常用的预报方法的试报结果作了比较。  相似文献