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Kanzok SM Fechner A Bauer H Ulschmid JK Müller HM Botella-Munoz J Schneuwly S Schirmer R Becker K 《Science (New York, N.Y.)》2001,291(5504):643-646
The disulfide reducing enzymes glutathione reductase and thioredoxin reductase are highly conserved among bacteria, fungi, worms, and mammals. These proteins maintain intracellular redox homeostasis to protect the organism from oxidative damage. Here we demonstrate the absence of glutathione reductase in Drosophila melanogaster, identify a new type of thioredoxin reductase, and provide evidence that a thioredoxin system supports GSSG reduction. Our data suggest that antioxidant defense in Drosophila, and probably in related insects, differs fundamentally from that in other organisms. 相似文献
56.
Colbourne JK Pfrender ME Gilbert D Thomas WK Tucker A Oakley TH Tokishita S Aerts A Arnold GJ Basu MK Bauer DJ Cáceres CE Carmel L Casola C Choi JH Detter JC Dong Q Dusheyko S Eads BD Fröhlich T Geiler-Samerotte KA Gerlach D Hatcher P Jogdeo S Krijgsveld J Kriventseva EV Kültz D Laforsch C Lindquist E Lopez J Manak JR Muller J Pangilinan J Patwardhan RP Pitluck S Pritham EJ Rechtsteiner A Rho M Rogozin IB Sakarya O Salamov A Schaack S Shapiro H Shiga Y Skalitzky C Smith Z Souvorov A Sung W 《Science (New York, N.Y.)》2011,331(6017):555-561
We describe the draft genome of the microcrustacean Daphnia pulex, which is only 200 megabases and contains at least 30,907 genes. The high gene count is a consequence of an elevated rate of gene duplication resulting in tandem gene clusters. More than a third of Daphnia's genes have no detectable homologs in any other available proteome, and the most amplified gene families are specific to the Daphnia lineage. The coexpansion of gene families interacting within metabolic pathways suggests that the maintenance of duplicated genes is not random, and the analysis of gene expression under different environmental conditions reveals that numerous paralogs acquire divergent expression patterns soon after duplication. Daphnia-specific genes, including many additional loci within sequenced regions that are otherwise devoid of annotations, are the most responsive genes to ecological challenges. 相似文献
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Axonal proteins of presynaptic neurons during synaptogenesis 总被引:5,自引:0,他引:5
P Sonderegger M C Fishman M Bokoum H C Bauer P G Nelson 《Science (New York, N.Y.)》1983,221(4617):1294-1297
Changes occur in the synthesis and axonal transport of neuronal proteins in dorsal-root ganglia axons as a result of contact with cells from the spinal cord during synapse formation. Dorsal-root ganglia cells were cultured in a compartmental cel culture system that allows separate access to neuronal cell bodies and their axons. When cells from the ventral spinal cord were cultured with the dorsal-root ganglia axons, synapses were established within a few days. Metabolic labeling and two-dimensional electrophoresis revealed that four of more than 300 axonal proteins had changed in their expression by the time synapses were established. The highly selective nature of these changes suggests that the proteins involved may be important in the processes of axon growth and synapse formation and their regulation by the regional environment. 相似文献
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Alexander Bruckner John Wright Christian Kampichler Roswitha Bauer Ellen Kandeler 《Biology and Fertility of Soils》1995,19(2-3):257-262
Equipment and handling methods for the preparation of soil mesocosms were developed. The mesocosms were used to investigate the interrelationships between mesofauna and microflora in a coniferous forest soil. Soil monoliths were taken from the ground, defaunated by deep-freezing, wrapped in nets to control reimmigration of different faunal size-classes, and replanted in the field for 8 months. in a practical test the technique described here proved to be an inexpensive field method for producing a replicated series of mesocosm in a short time. Deep-freezing is appropriate for defaunating soil monoliths. The fine nets effectively exluded meso-and macrofauna. No significant differences were found in the abundance of Enchytraeids and Collembola between recolonized mesocosms and the undisturbed control at the end of the study period. In contrast, oribatid mite abundace was still greatly reduced in the recolonized esocosms. Dominance structure and species composition of the more dominant oribatid species in the different treatments were apparently similar. To compensate for the low colonization ability of oribatids, a reintroduction of selected animal size-classes to defaunated monoliths is recommended. 相似文献
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Cotton management in a compacted subsurface microirrigated coastal plain soil of the southeastern US
A loamy sand Acrisol (Aquic Hapludult) that had been microirrigated for 6 years became so severely compacted that it had root limiting values of soil cone index in the Ap horizon and a genetic hardpan below it. Deep and surface tillage systems were evaluated for their ability to alleviate compaction. Deep tillage included subsoiling or none. Both deep tillage treatments were also surface tilled by disking, chiseling, or not tilling. Subsoiling was located in row or between rows to avoid microirrigation tubes (laterals) that were buried under every other mid row or every row. Cotton (Gossypium hirsutum) was planted in 0.96-m wide rows. Cotton yield was improved by irrigation from 485 to 1022 kg ha−1 because both 2001 and 2002 were dry years. Tillage loosened the soil by an average of 0.5–1.3 MPa; but compacted zones remained outside tilled areas. Subsoiling improved yield by 131 kg ha−1 when performed in row where laterals were placed in the mid rows; but subsoiling did not improve yield when it was performed in mid rows. For subsurface irrigation management in these soils, the treatment with laterals buried under every other mid row was able to accommodate in-row subsoiling which improved yield; and this treatment was just as productive as and had been shown to be less expensive to install than burying laterals under every row. 相似文献
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