排序方式： 共有166条查询结果，搜索用时 250 毫秒
51.
Melissa A. Fitzgerald Christine J. Bergman Adoracion P. Resurreccion Jürgen Mller Rosario Jimenez Russell F. Reinke Margrit Martin Pedro Blanco Federico Molina Ming‐Hsuan Chen Victoria Kuri Marissa V. Romero Fatemeh Habibi Takayuki Umemoto Supanee Jongdee Eduardo Graterol K. Radhika Reddy Priscila Zaczuk Bassinello Rajeswari Sivakami N. Shobha Rani Sanjukta Das Ya Jane Wang Siti Dewi Indrasari Asfaliza Ramli Rauf Ahmad Sharifa S. Dipti Lihong Xie Nguyen Thi Lang Pratibha Singh Dmaso Castillo Toro Fatemeh Tavasoli Christian Mestres 《Cereal Chemistry》2009,86(5):492-498
Amylose content is a parameter that correlates with the cooking behavior of rice. It is measured at the earliest possible stages of rice improvement programs to enable breeders to build the foundations of appropriate grain quality during cultivar development. Amylose is usually quantified by absorbance of the amylose‐iodine complex. The International Network for Quality Rice (INQR) conducted a survey to determine ways that amylose is measured, reproducibility between laboratories, and sources of variation. Each laboratory measured the amylose content of a set of 17 cultivars of rice. The study shows that five different versions of the iodine binding method are in use. The data show that repeatability was high within laboratories but reproducibility between laboratories was low. The major sources of variability were the way the standard curve was constructed and the iodine binding capacity of the potato amylose used to produce the standard. Reproducibility is much lower between laboratories using a standard curve of potato amylose alone compared with those using calibrated rice cultivars. This study highlights the need to standardize the way amylose is measured, and presents research avenues for doing so. 相似文献
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Abdullah Darzi-Naftchali Ali Shahnazari Fatemeh Karandish 《Archives of Agronomy and Soil Science》2016,62(11):1554-1566
This research was conducted to quantify total phosphorus (TP) losses in poorly drained-consolidated paddy fields equipped with different surface and shallow subsurface drainage systems including drain depth of 0.9 m and drain spacing of 30 m (D0.9L30), drain depth of 0.65 m and drain spacing of 30 m (D0.65L30), drain depth of 0.65 m and drain spacing of 15 m (D0.65L15), drain spacing of 15 m and drain depths of 0.65 and 0.9 m as alternate depths (Bilevel). Typical surface drainage system of consolidated paddy fields was also considered as conventional practice of the study area (control). The subsurface drained fields were under year-round crop production of rice-canola, while the surface drained fields experienced only rice cropping once a year. During three rice-canola-rice growing seasons, TP losses through drainage and leaching in the D0.9L30, Bilevel, D0.65L30, D0.65L15 and control treatments were respectively, 1.12, 0.98, 1.44, 1.53 and 24.48 kg ha?1, equivalent to about 3.9%, 3.4%, 5.1%, 5.3% and 85.7% of applied triple superphosphate fertilizer. In the rice growing seasons, TP losses through surface runoff were higher than those through subsurface drainage effluents. Shallow subsurface drainage systems were promising for the study area compared surface drainage as phosphorus risks were reduced by 79%, 77%, 64% and 57% through D0.9L30, Bilevel, D0.65L30 and D0.65L15, respectively. These results demonstrated that, by providing suitable condition for winter cropping, subsurface drainage systems can diminish concerns related to phosphorus losses from poorly drained paddy fields in the north of Iran. 相似文献
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Fatemeh Ebrahimi Mohsen Gholizadeh Ghodrat Rahimi-Mianji Ayoub Farhadi 《Tropical animal health and production》2017,49(8):1657-1662
Genome-wide association studies (GWAS) have introduced an influential tool in the search for quantitative trait loci (QTL) influencing economically important traits in sheep. To identify QTL associated with greasy fleece weight, a GWAS with 50 K single nucleotide polymorphisms (SNPs) was performed in a Baluchi sheep population. Association with greasy fleece weights was tested using the software Plink. The results of our GWAS provided three novel SNP markers and candidate genes associated with greasy fleece weight. A total of three chromosome-wide significant associations were detected for SNP on chromosomes 17 and 20 affecting greasy fleece weight across the four shearing. One of the significant SNP markers was located within ovine known genes namely FAM101A. Further investigation of these identified regions in validation studies will facilitate the identification of strong candidate genes for wool production in sheep. 相似文献
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Malekinejad Hassan Rahmani Fatemeh Bahrampour Kobra 《Tropical animal health and production》2010,42(6):1229-1234
Zearalenone (ZEA) as a mycoestrogen is found frequently in human foods and animal feeds. Its estrogenic effects depend on
its biotransformation fate including both first- and second-phase reactions, which are predominantly governed by hydroxylation
and glucuronidation, respectively. In this study, we investigate the hepatic biotransformation of ZEA in river buffalo. To
evaluate the hepatic biotransformation of ZEA, both subcellular fractions of the liver were prepared. ZEA was incubated with
intracellular subfractions in the presence of nicotinamide dinucleotide phosphate, and the products were determined by means
of high-performance liquid chromatography. Moreover, in the same frame of experiment and in the presence of uridine diphosphate
glucuronic acid, the rate of glucuronidation for substrate and products were estimated as well. We found that α-zearalenol
(α-ZOL) is the major hydroxylated hepatic metabolite of ZEA produced by both studied subcellular fractions. The enzymatic
kinetics analyses indicated that the α-ZOL and β-ZOL production by microsomal fraction were two- and three-fold higher than
those by postmitochondrial fraction, respectively. The calculated data showed that α-ZOL is conjugated with glucuronic acid
more than ZEA and β-ZOL, especially at the lower concentrations, which seems to be more applicable. Our data suggest that
unlike other domestic ruminants including cattle and sheep, the hepatic biotransformation of ZEA in river buffalo results
in bioactivation and formation of potent estrogenic metabolite. Moreover, at the relevant concentrations, the produced potent
estrogenic metabolite is entirely conjugated with glucuronic acid and, consequently, may cause the prolongation of presence
of the compound in the body due to enterohepatic cycle. 相似文献
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Fatemeh Davami Farnaz Eghbalpour Leila Nematollahi Farzaneh Barkhordari Fereidoun Mahboudi 《Iranian Biomedical Journal》2015,19(4):194-205
Background:
The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood.Methods:
In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes.Results:
In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium.Conclusion:
The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.Key Words: CHO cells, Culture media, Peptones, Recombinant proteins 相似文献58.
BACKGROUND: Trichophyton tonsurans is one of the dermatophyte fungi which invades the skin and hair of human. Several properties of this fungus have been investigated so far. However a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the Squalene epoxidase gene which is related to synthesis of ergosterol in this fungus. METHODS: Pairs of 23 and 24 nucleotides primers were designed from highly conserved regions of the similar genes in other fungi. Mentioned primers were utilized in PCR by using isolated genomic DNA of T. tonsurans whereas the PCR fragments were then sequenced. RESULTS AND CONCLUSION: Nucleotides (n = 558) have been sequenced from this new gene which encodes a polypeptide with 186 amino acids. Sequences comparison in gene data banks (NCBI, NIH) for this part of DNA and its deduced amino acid revealed significant homology with members of the eukaryotic Squalene epoxidase. 相似文献
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An in vitro regeneration system was developed using organogenic callus derived from in vitro grown cotyledonary explants of Gleditsia caspica Desf., an important leguminous tree. Murashige and Skoog (MS) basal medium augmented with 0.2 g L?1 myo-inositol and various concentrations of either 2,4-dichlorophenoxyacetic acid (2,4-D), naphthaleneacetic acid, or indole-3-butyric acid (IBA) alone as well as combined with cytokinins was used for callus induction. The highest frequency of organogenic yellowish-white and nodular callus (93 %) was obtained from explants grown on medium supplemented with 13.5 μM 2,4-D and 4.4 μM benzyladenine (BA). The yellowish-white and nodular callus when transferred to MS medium supplemented with BA (2.2–17.7 μM) or kinetin (KT; 2.3–18.8 μM) solely or in combination with 2.3 μM 2,4-D produced several microshoots after 5 weeks culture. The calli cultured on MS medium with 4.4 μM BA singly showed superior growth response and produced both maximum shoot regeneration (94 %) and the highest mean number (4.3) of microshoots per callus. Transfer of regenerated microshoots onto modified MS basal medium fortified with 5.8 μM gibberellic acid and 4.4 μM BA resulted in the maximum number of internodes per shoot and the highest shoot elongation after a period of 6 weeks. Optimum rooting of 90 %, an average 6.1 roots per shoot, and a mean root length of 3.6 cm was observed when half-strength MS medium was supplemented with 9.8 μM IBA and 0.92 μM KT. The regenerated healthy plants with well-developed shoots and roots showed a survival rate of 77 % after acclimatization and transplanting to garden soil for a 10-week hardening period under ex vitro conditions. 相似文献
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Farzaneh Noori Naser Agh Fatemeh Jafari Reza Jalili Enric Gisbert Mansour Torfi Mozanzadeh 《Aquaculture Nutrition》2019,25(5):1050-1062
The influence of dietary fish oil (FO) substitution with blends of vegetal oils (VO: canola, linseed, olive, sunflower, corn and coconut oils) in plant protein‐rich diets on reproductive performance and fatty acid dynamics of embryos was evaluated in female rainbow trout (Oncorhynchus mykiss) brooders (1.8 ± 0.1 kg). Four diets were formulated in which 20% (FO80/VO20), 50% (FO50/VO50), 75% (FO25/VO75) and 100% (VO100) of FO were replaced by mixture of VO. The above‐mentioned diets were administered for a short period prior to spawning (3 months). Fish fed the VO100 had the lowest fertilization rate (81.3 ± 2.3), whereas brooders fed the FO80/VO20 diet had the lowest survival rates at eyed embryo stage (83.7 ± 1.6%) and hatching rate (79.9 ± 3.1%). The fatty acid dynamics of embryos were not only affected by embryonic developmental stages, but also they were influenced by the dietary fatty acid profile. Our study confirmed that using blends of different VO sources and FM residual fat in the FO25/VO75 for the short‐term period prior to spawning seemed to be a good strategy in terms of successful reproduction for sparing high levels of FM and FO in diet of O. mykiss brooders. 相似文献