反向高效液相色谱法检测双峰驼血浆中对乙酰氨基酚的含量     

散仁  巴拉  岳伟东  哈斯苏荣 《中国畜牧兽医》2017,44(8):2517-2522

试验旨在建立测定双峰驼血浆中对乙酰氨基酚浓度的反相高效液相色谱法（reversed phase high-performance liquid chromatography,RP-HPLC）。选用Supelco Discovery C18色谱柱（150 mm×4.6 mm,5 μm）,以甲醇：水=20：80为流动相,流速为0.5 mL/min,紫外检测波长为248 nm,采用甲醇直接沉淀蛋白方法处理血浆样品。结果表明,对乙酰氨基酚的保留时间为6.5 min,分离度良好,无血浆杂峰干扰,且在0.08~10.24 μg/mL浓度范围内线性关系良好（R²=0.9997,n=8）,检测限为0.10 μg/mL,日内和日间精密度RSD均小于7.0%,绝对回收率为80.39%~93.56%,相对回收率为94.66%~104.73%。因此,本试验所建立的RP-HPLC法操作简便、准确,重复性好,专属性强,适用于检测双峰驼血浆中对乙酰氨基酚浓度,为后续的借助特异性探针药物--对乙酰氨基酚研究双峰驼CYP1A酶的体内活性提供了可靠而准确的定量方法。  相似文献   

Relationships among cultivated <Emphasis Type="Italic">Opuntia ficus</Emphasis>-<Emphasis Type="Italic">indica</Emphasis> genotypes and related species assessed by cytoplasmic markers     

Giuseppina Las Casas  Gaetano Distefano  Marco Caruso  Elisabetta Nicolosi  Alessandra Gentile  Stefano La Malfa 《Genetic Resources and Crop Evolution》2018,65(3):759-773

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The Effect of Drought Stress on Nutraceutical Properties of <Emphasis Type="Italic">Zea mays</Emphasis> Bran     

E. Lugo-Cruz  F. Zavala-García  H. Rodríguez-Fuentes  V. Urías-Orona  J. A. Vidales-Contreras  R. Carranza-De La Rosa  G. Niño-Medina 《Gesunde Pflanzen》2018,70(4):179-184

The effect of drought stress on total phenols, hydroxycinnamic acids, and antioxidant capacity in Cebú, DK2027, and DK2034 maize hybrids was evaluated. Compared to irrigation treatment, total phenols decreased in drought stress treatment by 35.34%, 5.59%, and 31.57% in Cebú, DK2027, and DK2034, respectively. In addition, the levels of t-ferulic, c-ferulic and p-coumaric acids decreased by 17.74%, 23.93%, and 29.83% in Cebú, by 8.92%, 8.62%, and 24.03% in DK2027, and by 13.66%, 11.03%, and 10.38% in DK2034, respectively. The predominant hydroxycinnamic acid from bran extracts was t-ferulic (67% to 72%), followed by c-ferulic (20% to 21%), and p-coumaric (7% to 11%) for both irrigation and drought stress treatments, while caffeic and sinapic acids were found in traces or were not detected. The antioxidant capacity decreased by 8%, 3%, and 9% in trolox equivalents and 7%, 17%, and 14% in percentage of radical scavenging activity for Cebú, DK2027, and DK2034 genotypes, respectively. The reduction of total phenols, hydroxycinnamic acids, and antioxidant capacity is due to the radical scavenging and photoprotective actions of hydroxycinnamic acids against highly reactive oxygen species generated under drought stress treatment.  相似文献   

偏心轮推杆行星传动的传动原理研究   总被引：5，自引：2，他引：3  

陶栋材  高英武  全腊珍  卢月娥 《湖南农业大学学报(自然科学版)》2000,26(4):314-317

提出并分析了偏心轮推杆行星传动的传动原理,建立了理论齿廓和实际齿廓方程：证明了定传动比采用不同的安装方式,可得到６种不同的传动比。  相似文献   

HMW—GS指印法速测杂交小麦种子纯度的适用性   总被引：2，自引：1，他引：1  

许钢垣  潘幸来 《麦类作物学报》2000,20(4):86-89

以１０％ＳＤＳＤ－ＰＡＧＥ法测试了１２个小麦杂交组合的２７粒Ｆ１种子,并对照测定了２０粒对应双亲种子,重点比较了其ＨＭＷ－ＧＳ（高分子量麦谷蛋白亚基）的显带情况。结果表明,⑴父母本ＨＭＷ－ＧＳ基因在Ｆ１籽粒中共显;⑵双亲至少有一条互异ＨＭＷ－ＧＳ带的组合,其Ｆ１是否为真杂种依此法可以鉴别;⑶父母本ＨＭＷ－ＧＳ带型完全相同的组合,其Ｆ１的真伪不能依此法判定。  相似文献   

Can pH and Water-to-Protein Ratio be Good Instruments to Evaluate the Abusive Water Added in Seafood by Phosphate Addition?     

Lyzandra Laís De Almeida Lemos 《Journal Of Aquatic Food Product Technology》2019,28(3):298-313

The aim of the present study was to evaluate the effect of the phosphates on tuna meat pH, and water-to-protein ratio. Tuna fillets were soaked in cold (5 °C) tripolyphosphate solution (STPP) and blend phosphate (CARNAL® 961) at 3, 5 and 10% for 30, 60 and 120 min. The control group was represented by cold distilled water. After soaking, the samples were drained, weighed, frozen, stored (-35 °C, for 15 days), thawed, drained, and grilled. After each step, samples were withdrawn for the following analysis: pH, phosphate (as P2O5), moisture and protein. A gradual increase in pH and residual phosphate was observed, which remained below the limits established by national (for pH) and international (for phosphate) legislation. A moderate positive correlation between pH and phosphate values was observed. An increase in moisture content and the water-to-protein ratio were observed, and still below to the legislation limit (i.e., 6.0). We suggest a critical review and possible changes in the parameters which are used as indicators of abusive use of the phosphate additive in seafood to combat the economic fraud in the frozen seafood products. It should be emphasized that these suggestions should be the focus of the collaborative study for its validation.  相似文献   

Gene expression kinetics of the yellow head virus in experimentally infected Litopenaeus vannamei     

Yenelli Cedano‐Thomas  Jorge De La Rosa‐Vélez  Jean Robert Bonami  Francisco Vargas‐Albores 《Aquaculture Research》2010,41(10):1432-1443

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耕作方式对东北黑土坡耕地土壤物理性状及根系垂直分布的影响          下载免费PDF全文

闫雷  喇乐鹏  董天浩  刘鸣一  孙小贺  孟庆尧  张钰莹  张乃文  孟庆峰 《农业工程学报》2021,37(1):125-132

为了明确耕作方式对东北薄层黑土坡耕地土壤物理性状以及玉米根系垂直分布的影响,该研究以东北薄层黑土坡耕地土壤为研究对象,设置免耕和翻耕2种耕作方式,研究耕作方式对不同坡位土壤水稳性团聚体粒径分布与稳定性、土壤容重、土壤孔隙度、土壤贯入阻力以及玉米根系在垂直方向主要分布深度的影响,并进一步探究各指标间的相互关系。结果表明,与免耕相比,翻耕处理通过增加土壤1～2 mm粒级水稳性团聚体含量,降低0～20 cm土层土壤容重,改善0～20cm土层土壤孔隙度,降低10～20cm土层土壤贯入阻力,增加玉米根系在垂直方向主要分布深度;但同时翻耕处理条件下5mm粒级土壤团聚体含量平均减少59.1%,平均重量直径平均降低31.9%。翻耕处理下,坡位对土壤孔隙度、5 mm粒级水稳性团聚体含量以及平均重量直径的影响都呈现坡中位与坡下位优于坡上位。研究结果表明,对于东北薄层黑土坡耕地,在坡中位和坡下位采用翻耕有利于降低土壤紧实度,促进玉米根系下扎;在坡上位可采用免耕改善土壤结构及其稳定性。该研究可为促进黑土可持续发展提供理论依据与参考。  相似文献   

Temporal changes in the fish community structure of a small subtropical spring lake: Decadal and seasonal analysis     

Arely Ramírez-García  Omar Domínguez-Domínguez  Eugenia López-López  Rodrigo Moncayo-Estrada  José De La Cruz-Agüero 《Ecology of Freshwater Fish》2023,32(1):53-63

In spring lakes, which have homogeneous environmental characteristics, it is expected that there will be no significant changes in the composition and structure of communities over time, and fluctuations will generally be related to the ecological attributes of the species. We studied the fish community in a small subtropical spring lake, Lake Zacapu, for two different decades to analyse its structural characteristics. Sampling was performed every 3 months at four sites from January to October 1995 and from May 2019 to May 2020. We determined the temporal variation (seasonal and decadal) in the fish community structure with respect to composition, abundance, diversity and dominance. We evaluated the association between species and water quality. Our results showed spatial homogeneity in most physicochemical variables that remained in the same range across decades, although some nutrients (NO₃) recently increased. All species identified in the early samples were present in the recent surveys (eleven native and two nonnative species), indicating that the fish community composition had significant qualitative stability. However, the dominant littoral cyprinid in 1995 was replaced by the more limnetic atherinopsid in 2019–2020, and the diversity and abundance of sensitive fish decreased in recent samplings. Consequently, the current status of critically endangered species is supported for some restricted and microendemic fish. The changes in the community structure attributes, which show a tendency for reduction in native species, emphasise the need to implement efforts to conserve freshwater fish diversity in small spring lakes.  相似文献