Diseases of the genital tract of indigenous cows in Bangladesh     

M. G. S. Alam  Abdur Rahman 《Tropical animal health and production》1979,11(1):179-180

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Attenuation of 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced gap junctional intercellular communication (GJIC) inhibition in MCF-10A cells by c9,t11-conjugated linoleic acid     

Rakib MA  Kim YS  Jang WJ  Choi BD  Kim JO  Kong IK  Ha YL 《Journal of agricultural and food chemistry》2010,58(22):12022-12030

The protective effect of c9,t11-conjugated linoleic acid (CLA) on the inhibition of gap junctional intercellular communication (GJIC) was examined in a human mammary epithelial cell line (MCF-10A) treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), relative to t10,c12-CLA isomer. TPA inhibited GJIC in a dose-dependent and reversible manner and was associated with connexin 43 phosphorylation. Pretreatment of 20 μM c9,t11-CLA for 24 h prior to 60 nM TPA for 1 h prevented the inhibition of GJIC by reducing the phosphorylation of connexin 43 via suppressing extracellular signal-regulated kinases (ERK1/2) activation. Reactive oxygen species (ROS) accumulation by TPA was attenuated by c9,t11-CLA. The efficacy of c9,t11-CLA in protecting inhibition of GJIC, connexin 43 phosphorylation, and ROS production was superior to that of t10,c12-CLA. These results suggest that c9,t11-CLA, including t10,c12-CLA, prevents the carcinogenesis of MCF-10A cells by protecting down-regulation of GJIC during the cancer promotion stage, and lack of their toxicities could be an excellent indicator for the chemoprevention of breast cancer.  相似文献   

Comparative mapping and discovery of segregation distortion and linkage disequilibrium across the known fragrance chromosomal regions in a rice F2 population     

Farahnaz Sadat Golestan Hashemi  Mohd Y. Rafii  Mohd Razi Ismail  Mahmud Tengku Muda Mohamed  Harun A. Rahim  Mohamad Abd Latif  Farzad Aslani 《Euphytica》2015,204(3):557-569

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薰衣草DXS基因的克隆、表达分析及原核表达     

龚林涛  苏秀娟  尹松松  孙明辉  闫博文  阿迪莱·阿布都热依木  陈全家 《新疆农业科学》2020,57(7):1233-1242

【目的】 研究拟克隆薰衣草DXS基因,并分析其表达,为揭示该基因在调控薰衣草萜类物质合成中的分子机理提供研究基础。【方法】 以薰衣草杂花为试材,同源克隆薰衣草DXS基因,进行基因序列分析、表达量比较和原核表达。【结果】 (1)薰衣草DXS基因开放阅读框长为2 181 bp,编码由726个氨基酸组成的蛋白质序列;薰衣草DXS蛋白等电点为6.57,分子量约为78.39 KDa,具有高度的保守性,与狭叶薰衣草、冬凌草、毛喉鞘蕊花的DXS蛋白亲缘关系相近;(2)DXS基因在杂花花器官的衰败期表达量最高,在法国蓝花器官的盛开期表达量最高,DXS基因在杂花花器官五个不同发育时期的表达量均高于法国蓝;DXS基因在杂花花萼中表达量最高,在法国蓝雄蕊中表达量最高,DXS基因在杂花花器官5个不同组织表达量均高于法国蓝(雌蕊、雄蕊除外);(3)在37℃、IPTG 0.8 mM条件下诱导4 h后,DXS蛋白表达量最大。【结论】 DXS基因表达量与薰衣草精油产量存在正相关关系。  相似文献   

Genetic variation for resistance to septoria tritici blotch in Iranian tetraploid wheat landraces     

Azadeh Ghaneie  Rahim Mehrabi  Naser Safaie  Masoud Abrinbana  Abbas Saidi  Mostafa Aghaee 《European journal of plant pathology / European Foundation for Plant Pathology》2012,132(2):191-202

Septoria tritici blotch (STB), caused by Mycosphaerella graminicola has recently become one of the devastating diseases in Iran causing significant yield losses on most commercial bread and durum wheat cultivars. Iran is located in the Fertile Crescent, a region where wheat was historically domesticated; and, thus, landraces derived from this region are of considerable global interest for identification of new sources of resistance to various stresses. Here, we report on the resistance responses of 45 tetraploid wheat landraces collected from different provinces of Iran to eight M. graminicola isolates. In total 138 isolate-specific resistances were found among all interactions (n = 360). The highest number of specific resistances (30 out of 32 interactions) was found in wheat landraces collected from West Azarbaijan. In contrast, all landraces from Kordestan were highly susceptible to M. graminicola isolates and only one isolate-specific resistance was identified among 106 isolate-wheat interactions. Kermanshah landraces showed the highest resistance variation against different isolates. About 57 isolate-specific resistances were identified among 104 interactions. Ilam landraces were highly resistant to STB as 28 specific resistances were observed among 32 interactions. Markazi (n = 2) and Sistan-Baluchestan (n = 1) were susceptible to all isolates tested. Landraces from Lorestan were generally susceptible to isolates tested as 26 susceptible responses were observed out of 32 interactions. Our results indicate that landraces collected from the Fertile Crescent region may possess diverse effective resistance genes or valuable broad spectrum resistance genes, and that their identification is of interest and can be exploited in breeding programs.  相似文献   

First report of <Emphasis Type="Italic">Neospora caninum</Emphasis> infection in cattle in Sudan     

Ibrahim AM  Elfahal AM  El Hussein AR 《Tropical animal health and production》2012,44(4):769-772

A cross-sectional survey was conducted in Sudan to determine sero-prevalence and risk factors associated with Neospora caninum infection in non-vaccinated dairy herds and to assess importance of the disease. Blood samples were collected from a total of 262 animals from 25 herds. Sera were tested for antibodies against N. caninum using ELISA test. The prevalence rates of N. caninum antibodies in cattle were high both at herd level (44%) and at individual animal level (10.7%). Herd level infection rates were similar in Khartoum State (43.7%) and at Gazira States (44.4%). The overall prevalence rates were higher (16.1%) in Gazira State than in Khartoum State (9%) but with no significant variation. The sero-prevalence at individual animal level was significantly higher (p < 0.05) in animals with history of abortion (12.8%) than in apparently healthy animal (11.3%), animal with history of infertility (8.1%), or neonatal death of calves (4.3%). In addition, significantly higher (P < 0.05) sero-prevalence was observed in samples collected during the rainy season (6.87%) than winter (3.05%) or summer (0.76%). However, no significant differences in sero-prevalence due to locality, animal breed, sex, and age were observed (p > 0.05). This preliminary study reveals for the first time the existence of natural N. caninum infection in Sudan. Also, the findings of the present study indicated that this disease is highly prevalent in two major areas of dairy production in the country, and this calls for control strategy to be implemented.  相似文献   

Natural durability of wood of ten native species from northeastern Mexico     

Artemio CARRILLO  Rahim FOROUGHBACHK  Verónica BUSTAMANTE  Christian WEHENKEL  Humberto GONZáLEZ 《中国林学(英文版)》2013,15(2):160-166

Thorn scrub vegetation in Mexico is distributed over 50 million ha, where native tree species are the source of forage, timber, firewood and charcoal. Research describing wood durability of species from this vegetation type has not been fully determined, nor classified according to international standards. Thus, the aim of this study was to determine and classify the natural durability of ten woody species. Their natural durability was determined according to the European Pre-Norm 807, the loss of dynamic modulus of elasticity (MOEdyn) (MPa) was determined and wood mass loss (g) after being exposed to Trametes versicolor and Coniophora puteana fungi. Wood durability was classified according to the European Norm 350-1. Highly significant differences (p<0.001) were found between the durability of woody species. The more durable species with lower MOEdyn lost were Condalia hookeri (57.5%±0.6%), Havardia pallens (58.2%±0.4%) and Acacia schaffneri (58.9%±6.3%). Species with lower mass loss after exposed to Coniophora puteana were Ebenopsis ebano (6.3%±1.9%), Condalia hookeri (8.6%±2.3%) and Cordia boissieri (11.8%±2.3%). E. ebano (7.1%±2.4%), Condalia hookeri (8.2%±2.5%) and Cordia boissieri (11.5%±3.1%) showed the lower mass lost after exposed to T. versicolor. According to European Norm 350-1, three woody species were classified as very durable and durable species.  相似文献   

Investigation on hydrostatic resistance and thermal performance of layered waterproof breathable fabrics     

Abdur Razzaque  Pavla Tesinova  Lubos Hes  Jana Salacova  Hafiz Affan Abid 《Fibers and Polymers》2017,18(10):1924-1930

Waterproof breathable layered fabrics allow water vapor passing through, but resist liquid water to pass. This ability of the fabrics to protect rain and snow water while allowing sweat vapor to evaporate from inside to outside atmosphere, leads them to be used as outdoor sportswear or protective clothing. The big challenge of enhanced hydrostatic resistance of these fabrics with proper breathability and thermal comfort has widened the research scope. This study presents an experimental investigation on hydrostatic resistance and thermal behavior of layered waterproof breathable fabrics. Six different types of hydrophobic and hydrophilic membrane laminated layered fabrics were evaluated by varying different fabric parameters in the experiment. Hydrostatic resistance and water vapor permeability of the laminated fabrics were measured by SDL ATLAS Hydrostatic Head Tester and PERMETEST respectively. Thermal properties were evaluated by ALAMBETA instrument. Moreover, FX-3300 air permeability tester was used to measure air permeability which represents the porosity of the fabrics and computer based See System software was used for water contact angle measurement on the outer fabric surface in order to determine the hydrophobic and hydrophilic properties. This experiment clearly discusses the influence of different fabric characteristics and parameters on hydrostatic resistance and thermal properties of the breathable laminated fabrics. The results show that fabric material composition, density, thickness, and hydrophobic and hydrophilic membranes have significant effects on hydrostatic resistance, breathability and thermal properties of different laminated fabrics.  相似文献   

Effects of thyroxine,cod liver oil and potassium iodide on growth and survival of juvenile seahorse,Hippocampus barbouri          下载免费PDF全文

Fatihah Abd Halid Nur  Annie Christianus  Abd Rahim Abdullah  Muta Harah Zakaria  Che Roos Saad 《Aquaculture Research》2018,49(2):867-873

Low survival at early stage is the bottleneck in seahorse aquaculture, particularly in the feeding aspect since newborn seahorses must feed immediately upon birth to sustain themselves. Seahorses are visual predator, therefore preferred live feed such as zooplankton. In aquaculture, the most common live feed used is Artemia. In this study, two experiments were conducted to determine the effect of different Artemia enrichment on the growth and survival of newborn Hippocampus barbouri. In the first experiment, six treatments using Artemia enriched with Culture Selco Plus? (SELCO), thyroxine (T4), potassium iodide (KI), cod liver oil (CLO), cod liver oil in combination with thyroxine (CLO + T4) and potassium iodide (CLO+KI) were fed to newborn H. barbouri. Newly hatched Artemia were used as control. At the end of first experiment, treatments using CLO + T4 produced juvenile H. barbouri with the best (p < .05) wet weight (0.142 ± 0.000 g), while juvenile in treatment CLO+KI recorded the highest (p < .05) standard length (3.947 ± 0.014 cm). Subsequently, a second experiment was carried out using the two best enrichment (CLO + T4 and CLO + KI) from the first experiment, but given at different frequency (daily, twice a week, once a week, once in 2 weeks). Daily enrichment using both CLO + T4 and CLO + KI showed no significant (p > .05) difference in growth performance and survival of juvenile of H. barbouri. Interestingly, juvenile fed CLO + T4 enriched Artemia at frequency of twice a week also has no significant difference (p > .05) in survival and growth performance (except for final standard length) when compared with treatment CLO + T4 at daily frequency.  相似文献   

Recurrent parent genome recovery in different populations with the introgression of Sub1 gene from a cross between MR219 and Swarna-Sub1     

Fahim Ahmed  Mohd Y. Rafii  Mohd Razi Ismail  Abdul Shukor Juraimi  Harun Abdul Rahim  Fatah Abro Tanweer  Mohammad Abdul Latif 《Euphytica》2016,207(3):605-618

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