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1.
M Murakami NWK Karja P Wongsrikeao B Agung M Taniguchi H Naoi T Otoi 《Reproduction in domestic animals》2005,40(6):511-515
This study was conducted to examine whether cat spermatozoa stored in ethanol for 1 month was capable of developing into pronuclei and of supporting normal embryonic development. In vitro matured oocytes were injected with frozen-thawed spermatozoa and ethanol-stored spermatozoa. The status of oocytes and sperm nuclei was examined at 4 and 18 h after injection of spermatozoa, and the presumptive zygotes were cultured for 7 days to assess the development of oocytes injected with each storage sperm. The percentage of enlarged sperm head at 4 h after injection was higher in ethanol-stored spermatozoa than in frozen-thawed spermatozoa, but there was no significant difference in the development of oocytes and sperm nuclei at 18 h after injection between the two groups. The development of oocytes to the blastocyst stage after injection of spermatozoa was observed only in oocytes with frozen-thawed spermatozoa. Of oocytes injected with ethanol-stored spermatozoa, two (2.8%) oocytes developed to the 16-cell stage. These results indicate that cat spermatozoa stored in ethanol can decondense and form male pronuclei after intracytoplasmic injection. However, the oocytes injected with ethanol-stored spermatozoa did not have the ability to develop to the blastocyst stage. 相似文献
2.
Zainal A. Muchlisin Putri I. Sarah Dhea F. Aldila Kartini Eriani Iwan Hasri Agung S. Batubara Firman M. Nur Mustaqim Mustaqim Cut Ruhul Muthmainnah Abinawanto Abinawanto Martin Wilkes 《Aquaculture Research》2020,51(4):1700-1705
Cryoprotectant is the crucial factor in the cryopreservation process. In general, there are two types of cryoprotectant, permeating and non‐permeating cryoprotectants. Dimethyl sulfoxide (DMSO) and egg yolk are common permeating and non‐permeating cryoprotectants respectively. Hence, the objective of the present study was to determine the best proportion of DMSO and egg yolk for the cryopreservation of Rasbora tawarensis sperm. A completely randomized experimental design was used in this study which involves two types of cryoprotectant and their combination at different concentrations, namely 5% DMSO, 5% egg yolk, 5% DMSO + 5% egg yolk and 2.5% DMSO + 2.5% egg yolk. Every treatment was conducted in three replicates. Combination of 5% DMSO + 5% egg yolk gave the best results cryoprotectant treatment had significant effects on sperm motility, fertilization and hatching rate of the R. tawarensis eggs (p < .05). It is concluded that the best proportion of cryoprotectants for sperm cryopreservation in this species is 5% DMSO + 5% egg yolk. 相似文献
3.
One of the first steps in estimating the potential for reducing emissions from deforestation and forest degradation (REDD) initiatives is the proper estimation of the carbon components. There are still considerable uncertainties about carbon stocks in tropical rain forest, coming essentially from poor knowledge of the quantity and spatial distribution of forest biomass at the landscape level. 相似文献
4.
Tesfaye Tesso Gebisa Ejeta Arun Chandrashekar Chia‐Ping Huang Agung Tandjung Mamadou Lewamy John D. Axtell Bruce R. Hamaker 《Cereal Chemistry》2006,83(2):194-201
Development of high‐protein digestibility (HPD)/high‐lysine (hl) sorghum mutant germplasm with good grain quality (i.e., hard endosperm texture) has been a major research objective at Purdue University. Progress toward achieving this objective, however, has been slow due to challenges posed by a combination of genetic and environmental factors. In this article, we report on the identification of a sorghum grain phenotype with a unique modified endosperm texture that has near‐normal hardness and possesses superior nutritional quality traits of high digestibility and enhanced lysine content. These modified endosperm lines were identified among F6 families developed from crosses between hard endosperm, normal nutritional quality sorghum lines, and improved HPD/hl sorghum mutant P721Q‐derived lines. A novel vitreous endosperm formation originated in the central portion of the kernel endosperm with opaque portions appearing both centrally and peripherally surrounding the vitreous portion. Kernels exhibiting modification showed a range of vitreous content from a slight interior section to one that filled out to the kernel periphery. Microstructure of the vitreous endosperm fraction was dramatically different from that of vitreous normal kernels in sorghum and in other cereals, in that polygonal starch granules were densely packed but without the typically associated continuous protein matrix. We speculate that, due to the lack of protein matrix, such vitreous endosperm may have more available starch for animal nutrition, and possibly have improved wet‐milling and dry‐grind ethanol processing properties. The new modified endosperm selections produce a range that approaches the density of the vitreous parent, and have lysine content and protein digestibility comparable to the HPD/hl opaque mutant parent. 相似文献
5.
Effect of the Removal of Cumulus Cells on the Nuclear Maturation, Fertilization and Development of Porcine Oocytes 总被引：7，自引：0，他引：7
P Wongsrikeao Y Kaneshige R Ooki M Taniguchi B Agung M Nii T Otoi 《Reproduction in domestic animals》2005,40(2):166-170
The present study was conducted to investigate the effects of attachment of cumulus cells to porcine oocytes during the process of maturation and fertilization on the nuclear maturation, fertilization and subsequent development after in vitro fertilization (IVF). In the first experiment, the cumulus cells were removed from cumulus-oocyte complexes (COCs) at 0, 24 and 42 h after the onset of maturation culture and were then cultured until reaching 42 h of cultivation. In the second experiment, COCs were denuded as described in the first experiment, then fertilized and cultured for 7 days. As a control, cumulus cells were allowed to maintain attachment to the oocytes until the end of IVF. The proportion of oocytes reaching metaphase II significantly increased with the delay in the removal treatment of cumulus cells. The proportion of normal fertilization gradually increased with delay in the removal treatment of cumulus cells from COCs until the end of IVF. However, no significant difference in the proportion of normal fertilization was found between the 42-h and control groups. The removal treatment of cumulus cells in the 0- and 24-h group significantly (p < 0.05) decreased the proportion of cleaved embryos when compared with the control, and none of them developed to the blastocyst stage. The proportion of development to the blastocyst stage was significantly higher (p < 0.05) in the control group than in the 42-h group (18.1% vs 12.4%; p < 0.05). The present study indicates that the attachment of cumulus cells to the oocyte during maturation and fertilization is important to support oocyte nuclear maturation, fertilization and subsequent embryo development. Particularly, the attachment of cumulus cells to the oocyte during IVF promotes embryonic development. 相似文献
6.
F Rajaei NWK Karja B Agung P Wongsrikeao M Taniguchi M Murakami R Sambuu M Nii T Otoi 《Reproduction in domestic animals》2005,40(5):429-432
The chemical toxicity of cryoprotectants to porcine embryos was examined by the evaluation of survival and DNA damage after exposure to cryoprotectants. Porcine blastocysts were exposed to 10% of ethylene glycol (EG), 1,2-propanediol (PD) or glycerol (GLY) for 1 h at room temperature (23-25 degrees C) and then cultured in vitro for 24 h. The survival rates of blastocysts exposed to PD and GLY were significantly lower than those of control blastocysts in which the embryos were exposed to carrier solution without cryoprotectants. Significantly more DNA-fragmented nuclei occurred in the cryoprotectant-exposed blastocysts, compared with the control blastocysts. Moreover, the indices of DNA-fragmented nuclei in the blastocysts without blastocoele re-formation after culture were significantly higher than those with blastocoele re-formation, irrespective of the exposure treatment. These results indicate that the exposure of porcine blastocysts to cryoprotectant decreases the survival rates and increases the DNA-fragmented nuclei in embryos. 相似文献
7.
Heritabilities (?2) and genetic correlations (rG) were estimated by regression of offspring on sire in two replicate, unselected lines of mice. Traits were associated with growth, feed efficiency, fat deposition and lean tissue. The ?2 for growth traits ranged from .34 to .42, except for 3-wk body weight, which was only .05. The ?2 for feed efficiency was .28. Ranges in ?2 were .45 to .50 for fat deposition traits and .36 to .42 for lean tissue traits. The rG involving 3-wk to 6-wk feed efficiency with hind carcass and fat measurements at 12 wk were small. Antagonisms were found between the sign of rG and the direction of usual breeding goals for pairs of traits (e.g., rG greater than 0 between fat deposition and hind carcass weight and rG less than 0 between hind carcass as a percentage of body weight and body weight). Selection indexes were developed to counteract these antagonisms. Modified selection indexes were compared where responses in individual traits rather than the aggregate breeding value were of major importance. The aggregate breeding values and selection indexes included: 1) epididymal fat pad weight and body weight, 2) hind carcass weight and body weight, or 3) all three traits. Economic weights in retrospect were calculated for the modified selection indexes. In some cases, expected correlated responses in component traits were not influenced greatly over a wide range of ratios of economic weights, but in other cases the component traits changed sharply over a narrow range of ratios. 相似文献
8.
Naoi H Otoi T Shimamura T Karja NW Agung B Shimizu R Taniguchi M Nagai T 《The Journal of reproduction and development》2007,53(2):271-277
We conducted two experiments to investigate the effects of storage temperature and period for cat ovaries on the meiotic and developmental competence of oocytes collected from the ovaries. In Experiment 1, ovaries were stored in physiological saline for 24 h at 4 C, 23-25 C, or 38 C (cold, room, and incubator temperature groups, respectively), and then oocytes were collected from the ovaries in each group. Morphologically intact oocytes were then selected and cultured in maturation medium for 24 h. Significantly more oocytes reached metaphase II (MII) in the cold temperature group (53.4%) than in the room and incubator temperature groups (20.0 and 2.4%, respectively). In Experiment 2, ovaries were stored in physiological saline at room temperature for 0, 6, 12 or 18 h, and then they were stored at 4 C (cold storage) until reaching a total storage period of 24 h. After storage of the ovaries, morphologically intact oocytes were matured, fertilized with frozen-thawed spermatozoa, and cultured in vitro. The rates of morphologically intact oocytes obtained from the ovaries stored at room temperature for 0, 6, 12 or 18 h were 35.3, 30.0, 26.4 and 14.7%, respectively, and the rates of intact oocytes that reached MII were 63.2, 36.4, 26.5 and 11.9%, respectively. The results suggested that the numbers of morphologically intact oocytes and intact oocytes that reached MII after in vitro maturation decrease gradually as the period of storage at room temperature before cold storage increases. Only oocytes from ovaries stored for 6 h developed to the blastocyst stage after in vitro maturation and fertilization when ovaries were stored at room temperature before cold storage. These results indicate that 24 h storage of ovaries at high temperatures (>23 C) decreases the meiotic competence of oocytes. The quality and developmental competence of oocytes are influenced by the length of storage at room temperature before cold storage. 相似文献
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10.
Pramudya Y. Onishi T. Senge M. Hiramatsu K. Nur Prasetyo M. R. 《Paddy and Water Environment》2019,17(3):331-338
In South Asia, an increasing population and frequent droughts have been significant factors deeply affecting water deficits in the region. In this study, recent drought conditions were evaluated by calculating the standardized precipitation index (SPI) for the period of 1991–2006, based on past data during the period from 1961 to 1990. In addition, the Thornthwaite equation was used to compute monthly potential evapotranspiration for the entire area of Indonesia. The APHRODITE data set was utilized for precipitation and temperature. Monthly rainfall data from April to September for 30 years (1961–1990) were used to obtain the gamma function for the computation of SPI values. Calculated probability of SPI for which values were < − 2 during the period from 1991 to 2006 was used to evaluate recent Indonesian drought conditions. Regarding potential soil water deficits, under the very simple assumption that the root zone is 30 cm, soil porosity is 0.4, and field capacity is 80% of soil porosity, the critical threshold of soil water deficit was set as − 96 mm. Frequency of potential water deficits < − 96 mm was counted during 1991–2006. The results of the SPI in Indonesia indicate that most parts of Indonesia have encountered severe and extreme drought for the period 1991–2006. Based on SPI interpretation, Borneo Island and West Papua are the islands that encountered the most extreme drought during the dry seasons. Borneo Island seems to have encountered extreme drought at the beginning and the middle of the dry seasons (April, May, and July). On the other hand, based on the Thornthwaite interpretation, Java and Bali Islands, and especially in Central Java and East Java, seem to have encountered the greatest soil water deficit at the middle and the end of the dry seasons (May, June, July, and September).
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